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The map label for this gene is sgcX [C]

Identifier: 23099787

GI number: 23099787

Start: 2387145

End: 2388203

Strand: Reverse

Name: sgcX [C]

Synonym: OB2332

Alternate gene names: 23099787

Gene position: 2388203-2387145 (Counterclockwise)

Preceding gene: 23099792

Following gene: 23099786

Centisome position: 65.78

GC content: 37.39

Gene sequence:

>1059_bases
ATGGAAAACTTACACTATGATTTTTTAATGGATTTACTAAAAACTCCATCTCCGTCTAGTATGGAAATGGATATTCAAAA
GAAGTGGATGGAATATGTGAAGACTTATGCTGATGAAGTGCGTACAGATAATGCCGGGAATGCAATTGCGGTATTAAATC
CAGAAGCTGAATTCAAAGTATTATTAGCAGGTCATTGCGATGAGATTGCATTAATAATAAATCGAATTGATGAAAATGGA
TTTCTCTACATTGATAAAATGGGGGGCATTAATCCAAAAGCAGCAGTTGGTATGAGAGTAGAAGTCCTGGGGTACCAAAA
CAAAATTACAGGGGTAATTGGTGTTAATGCTCAACATCACGGGGGATTAAAAGGTGATTTTGATTTAGAAGATCTATTTA
TTGATTGCGGGTTTACTTCTAAAAAAGAGGCAATTAAAAATGTGCAGATAGGTGATTTAGCTGTCTATAAAACAGAACCG
GAGTTACTGCAAAATAGATATATTGCGGGAAGAGGTTTAGACAATCGAACAGGAGCATTTATGGTTGCAGAAGTAATTCG
CATCTTATCAGAAAAAAATCTATCTATTGGTGTTTATGCGGTTAGCACCGTTAATGAGGAAACCAATATGGGCGGAGCGT
ATTTCGCTGCAGCCGGTATAGACCCAACGATGGCGATTGCCGTGGACGTTACGTTTGCAACAGATTATCCAAAAGTAAGT
AAAGAAAAATATGGTGAAGTATATTTAGAAGGTGGACCCGTTCTAGCAAAAGGTGCCCCAATTAATCGAAAAATAAATCT
ATGGCTGGAAAATGCAGCTAAGGAAGCAAATATAAAAATTCAATATGAACTAACACCACGTATGACAGGGACTGACGCTG
ACAGAATGCGACTTACTGGTAAAGGTGTTCCTGTATCATTAGTCTCATTACCATTACGTTATATGCATTCACCTGTGGAA
ACCGCGAGTAAACAGGATATGGAAGAAGAAGTGCAGCTAATGGTAACCATGTTAACTAATCTTTCAGGTAAGGAAAGTCT
AAATCCATTAGAAGATTAA

Upstream 100 bases:

>100_bases
TTTTACATATTTTGATATAGTTAGTGTACCCATTAATTTACGGATGGTAAACTAAAGTGCCTATAAACCGGTGATTACAG
TACTTAAGGAGGACGATTAA

Downstream 100 bases:

>100_bases
TTGGTAATAACAAAATAAAAAAATTTACTCTCTTCCGAAGTAGATGTAATACACTCTTTGGAAGGGAGTTTTTTTAATTT
CTTAATAATAATGATATATT

Product: endoglucanase

Products: D-glucose; GDP

Alternate protein names: NA

Number of amino acids: Translated: 352; Mature: 352

Protein sequence:

>352_residues
MENLHYDFLMDLLKTPSPSSMEMDIQKKWMEYVKTYADEVRTDNAGNAIAVLNPEAEFKVLLAGHCDEIALIINRIDENG
FLYIDKMGGINPKAAVGMRVEVLGYQNKITGVIGVNAQHHGGLKGDFDLEDLFIDCGFTSKKEAIKNVQIGDLAVYKTEP
ELLQNRYIAGRGLDNRTGAFMVAEVIRILSEKNLSIGVYAVSTVNEETNMGGAYFAAAGIDPTMAIAVDVTFATDYPKVS
KEKYGEVYLEGGPVLAKGAPINRKINLWLENAAKEANIKIQYELTPRMTGTDADRMRLTGKGVPVSLVSLPLRYMHSPVE
TASKQDMEEEVQLMVTMLTNLSGKESLNPLED

Sequences:

>Translated_352_residues
MENLHYDFLMDLLKTPSPSSMEMDIQKKWMEYVKTYADEVRTDNAGNAIAVLNPEAEFKVLLAGHCDEIALIINRIDENG
FLYIDKMGGINPKAAVGMRVEVLGYQNKITGVIGVNAQHHGGLKGDFDLEDLFIDCGFTSKKEAIKNVQIGDLAVYKTEP
ELLQNRYIAGRGLDNRTGAFMVAEVIRILSEKNLSIGVYAVSTVNEETNMGGAYFAAAGIDPTMAIAVDVTFATDYPKVS
KEKYGEVYLEGGPVLAKGAPINRKINLWLENAAKEANIKIQYELTPRMTGTDADRMRLTGKGVPVSLVSLPLRYMHSPVE
TASKQDMEEEVQLMVTMLTNLSGKESLNPLED
>Mature_352_residues
MENLHYDFLMDLLKTPSPSSMEMDIQKKWMEYVKTYADEVRTDNAGNAIAVLNPEAEFKVLLAGHCDEIALIINRIDENG
FLYIDKMGGINPKAAVGMRVEVLGYQNKITGVIGVNAQHHGGLKGDFDLEDLFIDCGFTSKKEAIKNVQIGDLAVYKTEP
ELLQNRYIAGRGLDNRTGAFMVAEVIRILSEKNLSIGVYAVSTVNEETNMGGAYFAAAGIDPTMAIAVDVTFATDYPKVS
KEKYGEVYLEGGPVLAKGAPINRKINLWLENAAKEANIKIQYELTPRMTGTDADRMRLTGKGVPVSLVSLPLRYMHSPVE
TASKQDMEEEVQLMVTMLTNLSGKESLNPLED

Specific function: Unknown

COG id: COG1363

COG function: function code G; Cellulase M and related proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M42 family [H]

Homologues:

Organism=Escherichia coli, GI87082417, Length=364, Percent_Identity=29.3956043956044, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI1790332, Length=350, Percent_Identity=28.5714285714286, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI1788727, Length=342, Percent_Identity=23.9766081871345, Blast_Score=106, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008007 [H]

Pfam domain/function: PF05343 Peptidase_M42 [H]

EC number: 3.2.1.4

Molecular weight: Translated: 38824; Mature: 38824

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENLHYDFLMDLLKTPSPSSMEMDIQKKWMEYVKTYADEVRTDNAGNAIAVLNPEAEFKV
CCCCCHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCEEE
LLAGHCDEIALIINRIDENGFLYIDKMGGINPKAAVGMRVEVLGYQNKITGVIGVNAQHH
EEECCCCCEEEEEEEECCCCEEEEECCCCCCCCHHCCEEEEEEECCCCEEEEEECCCCCC
GGLKGDFDLEDLFIDCGFTSKKEAIKNVQIGDLAVYKTEPELLQNRYIAGRGLDNRTGAF
CCCCCCCCHHHEEEECCCCCHHHHHHCCCCCCEEEEECCHHHHHCCEEECCCCCCCCCHH
MVAEVIRILSEKNLSIGVYAVSTVNEETNMGGAYFAAAGIDPTMAIAVDVTFATDYPKVS
HHHHHHHHHHCCCCEEEEEEEEEECCCCCCCCEEEEECCCCCEEEEEEEEEEECCCCCCC
KEKYGEVYLEGGPVLAKGAPINRKINLWLENAAKEANIKIQYELTPRMTGTDADRMRLTG
CCCCCEEEECCCEEEECCCCCCCEEEEEEECCCCCCCEEEEEEECCCCCCCCCHHEEEEC
KGVPVSLVSLPLRYMHSPVETASKQDMEEEVQLMVTMLTNLSGKESLNPLED
CCCCHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MENLHYDFLMDLLKTPSPSSMEMDIQKKWMEYVKTYADEVRTDNAGNAIAVLNPEAEFKV
CCCCCHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCEEE
LLAGHCDEIALIINRIDENGFLYIDKMGGINPKAAVGMRVEVLGYQNKITGVIGVNAQHH
EEECCCCCEEEEEEEECCCCEEEEECCCCCCCCHHCCEEEEEEECCCCEEEEEECCCCCC
GGLKGDFDLEDLFIDCGFTSKKEAIKNVQIGDLAVYKTEPELLQNRYIAGRGLDNRTGAF
CCCCCCCCHHHEEEECCCCCHHHHHHCCCCCCEEEEECCHHHHHCCEEECCCCCCCCCHH
MVAEVIRILSEKNLSIGVYAVSTVNEETNMGGAYFAAAGIDPTMAIAVDVTFATDYPKVS
HHHHHHHHHHCCCCEEEEEEEEEECCCCCCCCEEEEECCCCCEEEEEEEEEEECCCCCCC
KEKYGEVYLEGGPVLAKGAPINRKINLWLENAAKEANIKIQYELTPRMTGTDADRMRLTG
CCCCCEEEECCCEEEECCCCCCCEEEEEEECCCCCCCEEEEEEECCCCCCCCCHHEEEEC
KGVPVSLVSLPLRYMHSPVETASKQDMEEEVQLMVTMLTNLSGKESLNPLED
CCCCHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GDPglucose; H2O

Specific reaction: GDP-glucose + H2O = D-glucose + GDP

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]