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The map label for this gene is menF [H]

Identifier: 23099781

GI number: 23099781

Start: 2377017

End: 2378402

Strand: Reverse

Name: menF [H]

Synonym: OB2326

Alternate gene names: 23099781

Gene position: 2378402-2377017 (Counterclockwise)

Preceding gene: 23099784

Following gene: 23099780

Centisome position: 65.51

GC content: 35.06

Gene sequence:

>1386_bases
ATGATTGAGATAGAAGAGAAACGACTAGCGTCACTTATTGATATAACTATTAGTGAAATGGTATCGGAAGATGAAGCTAA
GTTGTTTAGTTACACAAAAAAAATTAACAAGCTAAATATATATACTTTTTTGGAATTAGCAGGAGATAGAGACCTAACAA
GAAGTTTCTGGTCCAGTAATTCAAACGACTTAACTATTCTTGGTGCTGGACAGGCTACTACCATACAAGCAAAAGGTGAA
CGTTTTCAATCAATAGAAAATCAGTGGAATGAATTGTTGAGAAAAGCTGTCATTCATAATCCTTTTGAAAAGCAAGGAAC
TGGAATTGTAGGACTAGGTGGTTTTGCATTTGACCCTAAAAAAGAATCAACACCTTTGTGGAGTAATTTTCCAGATGGTC
AATTAACTATACCTGAAATCATGGTTACGAGGATCGAAAATCAATTTTATTTAACTTTTAATATGTATCTGCGTACAGAT
GATCATGGTTTTCAATTAATGGAAGAATACCAAGAAATGGAGTCTTATTTATTAGAAGAATCGAGTTTCTTTAAAAAAGA
TGTAAATATGATCGAAAAGAAAGAAGTAGCTCCCAAACAATGGATGCAGACTATCCAAAAAGCAACTGAGGAAATGAAAA
CTTCCAGTACGAATAAGATTGTATTAGCAAGAGAGGTTCGTTTACGTTTTGATGAGCGAGCAGATATTCCATCCATTATA
AGAAAATTAATAGAAACGCAATCGAATAGTTATATTTTTGCTTATGAGATTGACGGAGATTGTTTTGTAGGGGCGACTCC
TGAACGTCTTGTTAAAGTGGAAGGAAATCAATTATTTTCTACTTGTCTGGCAGGTACAGCGCCAAGAGGAAAGACTACAA
AAGAAGATCAAAAAATTGCGGAAGAACTGTATAGAGATCCAAAGAATCGAAAAGAACATGACTATGTTGTTCAAATGATT
AGAAAAGCCGTTGAAAGGTACTGTACGGATGTAGATATTCCTAGTGAACCACAGGTGTATATGTATAAGACACTACAACA
TCTTTATACACCTGTAACGGCTAAATTAATGAATAGCCATAGTATTTTTTCTATTATAGAAGAACTTCATCCAACCCCAG
CTTTAGGTGGTACACCTAGAAAAGAATCGATGGCTTTTATTAGAGATAATGAACTGCTAGACAGAGGTTGGTACGGTGCT
CCTATTGGATGGTTAGATAGTAATAATAATGGTGAGTTTGCTGTAGCTATACGTTCTGCTATGATTCAAGGAACTGAAGC
GTCACTTTTTGCAGGTTGCGGAATTGTCGCAGATTCAGATCCAGTGAGTGAATATGAAGAAACAAATATTAAATTGATGC
CAATGCTCTCTGTTTTAGGAGGATAG

Upstream 100 bases:

>100_bases
TTTATAGAAATAATAGGCTTTTCTTGAGAAACTCGTAAAAAGAGCATAAAATAAGGAAGGATATAATTAATTTACGATAT
TATGTCGGGGGTTCATAGGA

Downstream 100 bases:

>100_bases
ACGATGGATTATACAGAAAAGTTAACGCGCTATGTCGCGAATTTTGTAGATGAATTAGCCAAAAGTGGTGTGACGGATGT
GGTGGTTTCTCCTGGATCTC

Product: menaquinone-specific isochorismate synthase

Products: NA

Alternate protein names: Isochorismate mutase [H]

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MIEIEEKRLASLIDITISEMVSEDEAKLFSYTKKINKLNIYTFLELAGDRDLTRSFWSSNSNDLTILGAGQATTIQAKGE
RFQSIENQWNELLRKAVIHNPFEKQGTGIVGLGGFAFDPKKESTPLWSNFPDGQLTIPEIMVTRIENQFYLTFNMYLRTD
DHGFQLMEEYQEMESYLLEESSFFKKDVNMIEKKEVAPKQWMQTIQKATEEMKTSSTNKIVLAREVRLRFDERADIPSII
RKLIETQSNSYIFAYEIDGDCFVGATPERLVKVEGNQLFSTCLAGTAPRGKTTKEDQKIAEELYRDPKNRKEHDYVVQMI
RKAVERYCTDVDIPSEPQVYMYKTLQHLYTPVTAKLMNSHSIFSIIEELHPTPALGGTPRKESMAFIRDNELLDRGWYGA
PIGWLDSNNNGEFAVAIRSAMIQGTEASLFAGCGIVADSDPVSEYEETNIKLMPMLSVLGG

Sequences:

>Translated_461_residues
MIEIEEKRLASLIDITISEMVSEDEAKLFSYTKKINKLNIYTFLELAGDRDLTRSFWSSNSNDLTILGAGQATTIQAKGE
RFQSIENQWNELLRKAVIHNPFEKQGTGIVGLGGFAFDPKKESTPLWSNFPDGQLTIPEIMVTRIENQFYLTFNMYLRTD
DHGFQLMEEYQEMESYLLEESSFFKKDVNMIEKKEVAPKQWMQTIQKATEEMKTSSTNKIVLAREVRLRFDERADIPSII
RKLIETQSNSYIFAYEIDGDCFVGATPERLVKVEGNQLFSTCLAGTAPRGKTTKEDQKIAEELYRDPKNRKEHDYVVQMI
RKAVERYCTDVDIPSEPQVYMYKTLQHLYTPVTAKLMNSHSIFSIIEELHPTPALGGTPRKESMAFIRDNELLDRGWYGA
PIGWLDSNNNGEFAVAIRSAMIQGTEASLFAGCGIVADSDPVSEYEETNIKLMPMLSVLGG
>Mature_461_residues
MIEIEEKRLASLIDITISEMVSEDEAKLFSYTKKINKLNIYTFLELAGDRDLTRSFWSSNSNDLTILGAGQATTIQAKGE
RFQSIENQWNELLRKAVIHNPFEKQGTGIVGLGGFAFDPKKESTPLWSNFPDGQLTIPEIMVTRIENQFYLTFNMYLRTD
DHGFQLMEEYQEMESYLLEESSFFKKDVNMIEKKEVAPKQWMQTIQKATEEMKTSSTNKIVLAREVRLRFDERADIPSII
RKLIETQSNSYIFAYEIDGDCFVGATPERLVKVEGNQLFSTCLAGTAPRGKTTKEDQKIAEELYRDPKNRKEHDYVVQMI
RKAVERYCTDVDIPSEPQVYMYKTLQHLYTPVTAKLMNSHSIFSIIEELHPTPALGGTPRKESMAFIRDNELLDRGWYGA
PIGWLDSNNNGEFAVAIRSAMIQGTEASLFAGCGIVADSDPVSEYEETNIKLMPMLSVLGG

Specific function: Siderophore biosynthesis; enterobactin biosynthesis. [C]

COG id: COG1169

COG function: function code HQ; Isochorismate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786809, Length=278, Percent_Identity=33.8129496402878, Blast_Score=171, Evalue=1e-43,
Organism=Escherichia coli, GI87082077, Length=260, Percent_Identity=32.3076923076923, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1787518, Length=228, Percent_Identity=27.1929824561404, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1788114, Length=217, Percent_Identity=26.2672811059908, Blast_Score=79, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6320935, Length=222, Percent_Identity=31.0810810810811, Blast_Score=99, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR015890
- InterPro:   IPR004561 [H]

Pfam domain/function: PF00425 Chorismate_bind [H]

EC number: =5.4.4.2 [H]

Molecular weight: Translated: 52521; Mature: 52521

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEIEEKRLASLIDITISEMVSEDEAKLFSYTKKINKLNIYTFLELAGDRDLTRSFWSSN
CCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCEEEEEEEEECCCCHHHHHHHCCC
SNDLTILGAGQATTIQAKGERFQSIENQWNELLRKAVIHNPFEKQGTGIVGLGGFAFDPK
CCCEEEEECCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCHHCCCEEEEECCCEECCC
KESTPLWSNFPDGQLTIPEIMVTRIENQFYLTFNMYLRTDDHGFQLMEEYQEMESYLLEE
CCCCCCCCCCCCCCEEHHHHHHHHHCCEEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHH
SSFFKKDVNMIEKKEVAPKQWMQTIQKATEEMKTSSTNKIVLAREVRLRFDERADIPSII
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHCCCCCCHHHHH
RKLIETQSNSYIFAYEIDGDCFVGATPERLVKVEGNQLFSTCLAGTAPRGKTTKEDQKIA
HHHHHCCCCCEEEEEEECCCEEECCCHHHEEEECCHHHHHHHHCCCCCCCCCCHHHHHHH
EELYRDPKNRKEHDYVVQMIRKAVERYCTDVDIPSEPQVYMYKTLQHLYTPVTAKLMNSH
HHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCH
SIFSIIEELHPTPALGGTPRKESMAFIRDNELLDRGWYGAPIGWLDSNNNGEFAVAIRSA
HHHHHHHHHCCCCCCCCCCCHHCEEEHCCCHHHHCCCCCCCCCEEECCCCCCEEEEEEHH
MIQGTEASLFAGCGIVADSDPVSEYEETNIKLMPMLSVLGG
HHCCCCCHHHHCCCEEECCCCCHHHHHCCEEEEEHHHHHCC
>Mature Secondary Structure
MIEIEEKRLASLIDITISEMVSEDEAKLFSYTKKINKLNIYTFLELAGDRDLTRSFWSSN
CCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCEEEEEEEEECCCCHHHHHHHCCC
SNDLTILGAGQATTIQAKGERFQSIENQWNELLRKAVIHNPFEKQGTGIVGLGGFAFDPK
CCCEEEEECCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCHHCCCEEEEECCCEECCC
KESTPLWSNFPDGQLTIPEIMVTRIENQFYLTFNMYLRTDDHGFQLMEEYQEMESYLLEE
CCCCCCCCCCCCCCEEHHHHHHHHHCCEEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHH
SSFFKKDVNMIEKKEVAPKQWMQTIQKATEEMKTSSTNKIVLAREVRLRFDERADIPSII
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHCCCCCCHHHHH
RKLIETQSNSYIFAYEIDGDCFVGATPERLVKVEGNQLFSTCLAGTAPRGKTTKEDQKIA
HHHHHCCCCCEEEEEEECCCEEECCCHHHEEEECCHHHHHHHHCCCCCCCCCCHHHHHHH
EELYRDPKNRKEHDYVVQMIRKAVERYCTDVDIPSEPQVYMYKTLQHLYTPVTAKLMNSH
HHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCH
SIFSIIEELHPTPALGGTPRKESMAFIRDNELLDRGWYGAPIGWLDSNNNGEFAVAIRSA
HHHHHHHHHCCCCCCCCCCCHHCEEEHCCCHHHHCCCCCCCCCEEECCCCCCEEEEEEHH
MIQGTEASLFAGCGIVADSDPVSEYEETNIKLMPMLSVLGG
HHCCCCCHHHHCCCEEECCCCCHHHHHCCEEEEEHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1629163; 8566759; 9387221; 9384377; 3131310 [H]