Click here to switch to the map view.

The map label for this gene is menD

Identifier: 23099780

GI number: 23099780

Start: 2375274

End: 2377013

Strand: Reverse

Name: menD

Synonym: OB2325

Alternate gene names: 23099780

Gene position: 2377013-2375274 (Counterclockwise)

Preceding gene: 23099781

Following gene: 23099779

Centisome position: 65.47

GC content: 39.08

Gene sequence:

>1740_bases
ATGGATTATACAGAAAAGTTAACGCGCTATGTCGCGAATTTTGTAGATGAATTAGCCAAAAGTGGTGTGACGGATGTGGT
GGTTTCTCCTGGATCTCGTTCTACACCACTTGCTCTTGTATTTACAGAGCATCCATCCATAAAAGAGTGGATTATCGTCG
ATGAGCGTTCAGCAGCCTTTTTTGCATTAGGATTAGCGAAGAAATCAAATCGTGCAGTAGCGATAGTATGTACGTCAGGA
ACAGCAGCAGCTAACTATTATCCTGCAATAGTGGAAGCACACTATAGTCGTGTACCACTTCTTGTATTAACAGCTGATCG
TCCGCATGAGCTGAGACATATTGGAGCTCCTCAAACAATTGAACAAATTAAAATGTATGGCGACTACACCAAATGGTTTC
ACGAAATGGCTATGCCGGAAGCATCAGATAAGATGTTACATTATGTTCGTAATAAAGCATCTCATGCGAAGCACGTTGCT
GAGGAAGGGAATCCTGGGGTTGTTCATTTAAATTTCCCATTACGCGAACCGTTAACACCTAATTTTTCTTTGGATGCTAT
TTGGGGTACAAAAAGTTATACTAGACAAAATATTATGCAAGAGGGAACCAAACAATTAGCGCCAAACCAATTGCAAATAT
TACTAGAGGAAGTCGGTCCAAACAAAAAAGGATTGTTTGTTTGTGGGCCACAGGCAGATGCTGAGTTTGCGGATGCGGTT
ACTTCATTAGCTGGGAAGTGGGGAATTCCTGTAGTTGCAGATCCTTTATCACAATTGAGAACGGGACAGCATCATAAGGA
CAATGTCATAGATGGGTACGATGCATTCCTAAGAGAAGCAGAAATTAGACAAGAGCTCAAACCGGATTATATTATTCGAT
TTGGAGCTATGCCAGTTTCTAAATCGTATTTATTTTATGTTATGGAGAATGTCGAAACACAACAATACGTAATCGAACCC
AACGAAGGAATTCGAGATCATTCTTCTAATCAAACAACATTTTTATTTGCCGATCCTACAACATTATGCCAACAACTTGA
GCAGATGACGGTGGTGGATCAAGATGTCTCTACTGCATGGTTGAAAACTTGGCAAGAAATGAATCAAATTGCTAAACATT
ACCTACTAGAGGGTGTCGAAGAACAAATAACGGAAGGCGAAGCCGTTAGAGGTTTAGCTGAAGTTATCCCAGATGGGAGC
ACCTTGTATGTTGGAAATAGCATGGCAATTCGGGATGTGGATACATTTTATATGACTTCACCAAAAACAATAAATATATT
AGCGAATCGTGGTGCTAATGGTATTGATGGTATGGTTTCAAGTGGAGTAGGAGCTTCTGCAGATAATGAGAGAGTAACAT
TATTACTCGGCGACTTATCTTTATTTCACGACATGAATGGATTATTTGCTGCTAAGCATTATAAGTTACCAATTACTATT
GTGTTAATTAATAATAATGGCGGCGGGATATTCTCCTTCTTACCACAAGCGAAAGATAAGCGTCATTTTGAAGCTTTGTT
TGGCACTCCAATGGATTTATCTTTTGAACAGGTAGCGAAACTGTATGAGGCAAATTACAATCATGTGAAAACAGAGGAAC
AATTAAAAGCTGCTTTGTACGAAAGTTATCAACTCGATGGTCTATCCATTATAGAAGTAAAAACAGATCGTGAACAAAAT
GTTCAATGGCATCAAGCGAAATGGCAATTGATTAAGGAAGCAATTTTAAAGGATGGTTAG

Upstream 100 bases:

>100_bases
TGCAGGTTGCGGAATTGTCGCAGATTCAGATCCAGTGAGTGAATATGAAGAAACAAATATTAAATTGATGCCAATGCTCT
CTGTTTTAGGAGGATAGACG

Downstream 100 bases:

>100_bases
ATGAGTTTAGATGATAAAGCGTTGCATTTTGAAGTAGTTGGTGATGGTGAACCAATCGTGTTATTACATGGTTTTACAGG
GACACTCAATACTTGGGAAT

Product: 2-oxoglutarate decarboxylase

Products: NA

Alternate protein names: SEPHCHC synthase; Menaquinone biosynthesis protein menD

Number of amino acids: Translated: 579; Mature: 579

Protein sequence:

>579_residues
MDYTEKLTRYVANFVDELAKSGVTDVVVSPGSRSTPLALVFTEHPSIKEWIIVDERSAAFFALGLAKKSNRAVAIVCTSG
TAAANYYPAIVEAHYSRVPLLVLTADRPHELRHIGAPQTIEQIKMYGDYTKWFHEMAMPEASDKMLHYVRNKASHAKHVA
EEGNPGVVHLNFPLREPLTPNFSLDAIWGTKSYTRQNIMQEGTKQLAPNQLQILLEEVGPNKKGLFVCGPQADAEFADAV
TSLAGKWGIPVVADPLSQLRTGQHHKDNVIDGYDAFLREAEIRQELKPDYIIRFGAMPVSKSYLFYVMENVETQQYVIEP
NEGIRDHSSNQTTFLFADPTTLCQQLEQMTVVDQDVSTAWLKTWQEMNQIAKHYLLEGVEEQITEGEAVRGLAEVIPDGS
TLYVGNSMAIRDVDTFYMTSPKTINILANRGANGIDGMVSSGVGASADNERVTLLLGDLSLFHDMNGLFAAKHYKLPITI
VLINNNGGGIFSFLPQAKDKRHFEALFGTPMDLSFEQVAKLYEANYNHVKTEEQLKAALYESYQLDGLSIIEVKTDREQN
VQWHQAKWQLIKEAILKDG

Sequences:

>Translated_579_residues
MDYTEKLTRYVANFVDELAKSGVTDVVVSPGSRSTPLALVFTEHPSIKEWIIVDERSAAFFALGLAKKSNRAVAIVCTSG
TAAANYYPAIVEAHYSRVPLLVLTADRPHELRHIGAPQTIEQIKMYGDYTKWFHEMAMPEASDKMLHYVRNKASHAKHVA
EEGNPGVVHLNFPLREPLTPNFSLDAIWGTKSYTRQNIMQEGTKQLAPNQLQILLEEVGPNKKGLFVCGPQADAEFADAV
TSLAGKWGIPVVADPLSQLRTGQHHKDNVIDGYDAFLREAEIRQELKPDYIIRFGAMPVSKSYLFYVMENVETQQYVIEP
NEGIRDHSSNQTTFLFADPTTLCQQLEQMTVVDQDVSTAWLKTWQEMNQIAKHYLLEGVEEQITEGEAVRGLAEVIPDGS
TLYVGNSMAIRDVDTFYMTSPKTINILANRGANGIDGMVSSGVGASADNERVTLLLGDLSLFHDMNGLFAAKHYKLPITI
VLINNNGGGIFSFLPQAKDKRHFEALFGTPMDLSFEQVAKLYEANYNHVKTEEQLKAALYESYQLDGLSIIEVKTDREQN
VQWHQAKWQLIKEAILKDG
>Mature_579_residues
MDYTEKLTRYVANFVDELAKSGVTDVVVSPGSRSTPLALVFTEHPSIKEWIIVDERSAAFFALGLAKKSNRAVAIVCTSG
TAAANYYPAIVEAHYSRVPLLVLTADRPHELRHIGAPQTIEQIKMYGDYTKWFHEMAMPEASDKMLHYVRNKASHAKHVA
EEGNPGVVHLNFPLREPLTPNFSLDAIWGTKSYTRQNIMQEGTKQLAPNQLQILLEEVGPNKKGLFVCGPQADAEFADAV
TSLAGKWGIPVVADPLSQLRTGQHHKDNVIDGYDAFLREAEIRQELKPDYIIRFGAMPVSKSYLFYVMENVETQQYVIEP
NEGIRDHSSNQTTFLFADPTTLCQQLEQMTVVDQDVSTAWLKTWQEMNQIAKHYLLEGVEEQITEGEAVRGLAEVIPDGS
TLYVGNSMAIRDVDTFYMTSPKTINILANRGANGIDGMVSSGVGASADNERVTLLLGDLSLFHDMNGLFAAKHYKLPITI
VLINNNGGGIFSFLPQAKDKRHFEALFGTPMDLSFEQVAKLYEANYNHVKTEEQLKAALYESYQLDGLSIIEVKTDREQN
VQWHQAKWQLIKEAILKDG

Specific function: Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-ca

COG id: COG1165

COG function: function code H; 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family. MenD subfamily

Homologues:

Organism=Escherichia coli, GI1788599, Length=566, Percent_Identity=28.6219081272085, Blast_Score=218, Evalue=1e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MEND_OCEIH (Q8CZD8)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_693246.1
- ProteinModelPortal:   Q8CZD8
- GeneID:   1015457
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB2325
- NMPDR:   fig|221109.1.peg.2322
- HOGENOM:   HBG634405
- OMA:   FYHDLNG
- BioCyc:   OIHE221109:OB2325-MONOMER
- HAMAP:   MF_01659
- InterPro:   IPR004433
- InterPro:   IPR012001
- InterPro:   IPR011766
- TIGRFAMs:   TIGR00173

Pfam domain/function: PF02775 TPP_enzyme_C; PF02776 TPP_enzyme_N

EC number: =2.2.1.9

Molecular weight: Translated: 64714; Mature: 64714

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDYTEKLTRYVANFVDELAKSGVTDVVVSPGSRSTPLALVFTEHPSIKEWIIVDERSAAF
CCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCEEEEEECCCCCCEEEEEECCCCEE
FALGLAKKSNRAVAIVCTSGTAAANYYPAIVEAHYSRVPLLVLTADRPHELRHIGAPQTI
EEEEECCCCCCEEEEEEECCCCCCCCCHHHHHHHCCCCCEEEEECCCCHHHHHCCCCHHH
EQIKMYGDYTKWFHEMAMPEASDKMLHYVRNKASHAKHVAEEGNPGVVHLNFPLREPLTP
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCC
NFSLDAIWGTKSYTRQNIMQEGTKQLAPNQLQILLEEVGPNKKGLFVCGPQADAEFADAV
CCEEEEEECCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHH
TSLAGKWGIPVVADPLSQLRTGQHHKDNVIDGYDAFLREAEIRQELKPDYIIRFGAMPVS
HHHHCCCCCCHHHCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCC
KSYLFYVMENVETQQYVIEPNEGIRDHSSNQTTFLFADPTTLCQQLEQMTVVDQDVSTAW
CCEEEEEEECCCCCEEEECCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHCCCHHHHH
LKTWQEMNQIAKHYLLEGVEEQITEGEAVRGLAEVIPDGSTLYVGNSMAIRDVDTFYMTS
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCEEEECCCEEEEECCEEEECC
PKTINILANRGANGIDGMVSSGVGASADNERVTLLLGDLSLFHDMNGLFAAKHYKLPITI
CCEEEEEECCCCCCCCHHHHCCCCCCCCCCEEEEEEECHHHHHCCCCCEEEEEEECEEEE
VLINNNGGGIFSFLPQAKDKRHFEALFGTPMDLSFEQVAKLYEANYNHVKTEEQLKAALY
EEEECCCCEEEEECCCCCCHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHH
ESYQLDGLSIIEVKTDREQNVQWHQAKWQLIKEAILKDG
HHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDYTEKLTRYVANFVDELAKSGVTDVVVSPGSRSTPLALVFTEHPSIKEWIIVDERSAAF
CCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCEEEEEECCCCCCEEEEEECCCCEE
FALGLAKKSNRAVAIVCTSGTAAANYYPAIVEAHYSRVPLLVLTADRPHELRHIGAPQTI
EEEEECCCCCCEEEEEEECCCCCCCCCHHHHHHHCCCCCEEEEECCCCHHHHHCCCCHHH
EQIKMYGDYTKWFHEMAMPEASDKMLHYVRNKASHAKHVAEEGNPGVVHLNFPLREPLTP
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCC
NFSLDAIWGTKSYTRQNIMQEGTKQLAPNQLQILLEEVGPNKKGLFVCGPQADAEFADAV
CCEEEEEECCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHH
TSLAGKWGIPVVADPLSQLRTGQHHKDNVIDGYDAFLREAEIRQELKPDYIIRFGAMPVS
HHHHCCCCCCHHHCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCC
KSYLFYVMENVETQQYVIEPNEGIRDHSSNQTTFLFADPTTLCQQLEQMTVVDQDVSTAW
CCEEEEEEECCCCCEEEECCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHCCCHHHHH
LKTWQEMNQIAKHYLLEGVEEQITEGEAVRGLAEVIPDGSTLYVGNSMAIRDVDTFYMTS
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCEEEECCCEEEEECCEEEECC
PKTINILANRGANGIDGMVSSGVGASADNERVTLLLGDLSLFHDMNGLFAAKHYKLPITI
CCEEEEEECCCCCCCCHHHHCCCCCCCCCCEEEEEEECHHHHHCCCCCEEEEEEECEEEE
VLINNNGGGIFSFLPQAKDKRHFEALFGTPMDLSFEQVAKLYEANYNHVKTEEQLKAALY
EEEECCCCEEEEECCCCCCHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHH
ESYQLDGLSIIEVKTDREQNVQWHQAKWQLIKEAILKDG
HHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12235376