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The map label for this gene is metK

Identifier: 23099769

GI number: 23099769

Start: 2364033

End: 2365229

Strand: Reverse

Name: metK

Synonym: OB2314

Alternate gene names: 23099769

Gene position: 2365229-2364033 (Counterclockwise)

Preceding gene: 23099774

Following gene: 23099768

Centisome position: 65.15

GC content: 38.43

Gene sequence:

>1197_bases
ATGGCTGCAAATCGACGTTTATTTACTTCAGAGTCAGTAACTGAGGGGCATCCAGATAAAATGTCTGACCAAATATCAGA
TGCTATTTTAGATGAAATTTTAAAGAAAGATCCAAACGCAAGGGTTGCATGTGAGACAACAGTAACTACAGGACTAGTTC
TTGTATCAGGAGAAATTTCTACTTCTACTTATGTAGATATACCAGCCCTTGTTCGTGAAACAGTAAAAGAAATAGGGTAT
ACACGTGCTAAATATGGTTTTGATTTTGAAACTTGCGCGGTATTAACTGCGATAGATGAACAGTCTGCAGATATTGCAGG
AGGAGTAAATCAAGCACTTGAGGCACGACAAGGAAAAATGAGTGAGGAAGAAATTGATGCTATTGGAGCTGGCGACCAAG
GGCTAATGTTTGGTTACGCATGTGATGAGACTGAAGAATTAATGCCTTTACCAATCTCATTAGCACATCAATTGTCCAAA
AGGTTAGCGGATGTAAGAAAAGAAGGTATAGTAGATTATCTTCGTCCAGATGGAAAGACACAAGTAACTGTTGAGTATGA
TGAGAATGACAAGCCCGAACGTATAGATACAATCGTTATCTCTACACAACATCATTCAGAAATACAAAATGAGCAAATTG
AAAGAGATATGATTGAAAAAGTCATTCAAGCGGTTGTTCCAAGCGAATTGTTAGATGATAAAACAAAATATTTTATAAAT
CCAACTGGTCGTTTTGTAATTGGAGGACCTCAAGGTGATGTAGGGCTGACAGGAAGAAAGATTATTGTAGACACGTACGG
TGGTTATGCTAGACATGGCGGAGGTGCATTTAGTGGCAAAGACGCTACAAAAGTAGACCGTTCTGCAGCATATGCAGCTC
GTTATGTTGCTAAGAATATAGTTGCCGCAGGACTTGCAAAATCTTGTGAGGTTCAATTAGCCTATGCCATTGGTGTCGCT
CAACCTGTCTCCATTGCAGTTAATACATTTGGAACTGGAAAAGTAAGTGAAGACAGATTGGTAGAAGCAGTTAGAGAATT
ATTTGATCTCCGTCCAGCTGGTATTATCCGCATGTTAGATTTAAGAAAACCTATATACAAAAATACCGCAGCTTATGGTC
ACTTTGGTCGAAAAGATATCTTATTCCCATGGGAAAAAACAGACAAAACAGAAGAGCTTATTGCTCTAACTAAATAA

Upstream 100 bases:

>100_bases
ACCGCGTTTTTTAGTACGAGGTTCTTTTATTGGCACTTTGAATAGGATAGAAGTTATAAAGAGATCCGTACCAACATATA
TCAAAGGAGAGTTTAGCCTT

Downstream 100 bases:

>100_bases
TTAAATGTTTAAAAAAACTATCATCTATCTTTAAGGTGGTAGTTTTTTTAGTTTATTAATCGAGTGGACCAACAGTTTGA
TTCCATTCGAAATTATTATT

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase

Number of amino acids: Translated: 398; Mature: 397

Protein sequence:

>398_residues
MAANRRLFTSESVTEGHPDKMSDQISDAILDEILKKDPNARVACETTVTTGLVLVSGEISTSTYVDIPALVRETVKEIGY
TRAKYGFDFETCAVLTAIDEQSADIAGGVNQALEARQGKMSEEEIDAIGAGDQGLMFGYACDETEELMPLPISLAHQLSK
RLADVRKEGIVDYLRPDGKTQVTVEYDENDKPERIDTIVISTQHHSEIQNEQIERDMIEKVIQAVVPSELLDDKTKYFIN
PTGRFVIGGPQGDVGLTGRKIIVDTYGGYARHGGGAFSGKDATKVDRSAAYAARYVAKNIVAAGLAKSCEVQLAYAIGVA
QPVSIAVNTFGTGKVSEDRLVEAVRELFDLRPAGIIRMLDLRKPIYKNTAAYGHFGRKDILFPWEKTDKTEELIALTK

Sequences:

>Translated_398_residues
MAANRRLFTSESVTEGHPDKMSDQISDAILDEILKKDPNARVACETTVTTGLVLVSGEISTSTYVDIPALVRETVKEIGY
TRAKYGFDFETCAVLTAIDEQSADIAGGVNQALEARQGKMSEEEIDAIGAGDQGLMFGYACDETEELMPLPISLAHQLSK
RLADVRKEGIVDYLRPDGKTQVTVEYDENDKPERIDTIVISTQHHSEIQNEQIERDMIEKVIQAVVPSELLDDKTKYFIN
PTGRFVIGGPQGDVGLTGRKIIVDTYGGYARHGGGAFSGKDATKVDRSAAYAARYVAKNIVAAGLAKSCEVQLAYAIGVA
QPVSIAVNTFGTGKVSEDRLVEAVRELFDLRPAGIIRMLDLRKPIYKNTAAYGHFGRKDILFPWEKTDKTEELIALTK
>Mature_397_residues
AANRRLFTSESVTEGHPDKMSDQISDAILDEILKKDPNARVACETTVTTGLVLVSGEISTSTYVDIPALVRETVKEIGYT
RAKYGFDFETCAVLTAIDEQSADIAGGVNQALEARQGKMSEEEIDAIGAGDQGLMFGYACDETEELMPLPISLAHQLSKR
LADVRKEGIVDYLRPDGKTQVTVEYDENDKPERIDTIVISTQHHSEIQNEQIERDMIEKVIQAVVPSELLDDKTKYFINP
TGRFVIGGPQGDVGLTGRKIIVDTYGGYARHGGGAFSGKDATKVDRSAAYAARYVAKNIVAAGLAKSCEVQLAYAIGVAQ
PVSIAVNTFGTGKVSEDRLVEAVRELFDLRPAGIIRMLDLRKPIYKNTAAYGHFGRKDILFPWEKTDKTEELIALTK

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family

Homologues:

Organism=Homo sapiens, GI4557737, Length=382, Percent_Identity=59.1623036649215, Blast_Score=438, Evalue=1e-123,
Organism=Homo sapiens, GI5174529, Length=382, Percent_Identity=58.6387434554974, Blast_Score=427, Evalue=1e-120,
Organism=Escherichia coli, GI1789311, Length=389, Percent_Identity=62.9820051413882, Blast_Score=497, Evalue=1e-142,
Organism=Caenorhabditis elegans, GI17538498, Length=389, Percent_Identity=55.2699228791774, Blast_Score=433, Evalue=1e-122,
Organism=Caenorhabditis elegans, GI17538494, Length=389, Percent_Identity=55.012853470437, Blast_Score=432, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI25145633, Length=392, Percent_Identity=54.5918367346939, Blast_Score=427, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI17551082, Length=387, Percent_Identity=52.1963824289406, Blast_Score=407, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI32565905, Length=389, Percent_Identity=50.6426735218509, Blast_Score=375, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI32565911, Length=338, Percent_Identity=53.5502958579882, Blast_Score=361, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI25145635, Length=341, Percent_Identity=52.4926686217009, Blast_Score=352, Evalue=2e-97,
Organism=Caenorhabditis elegans, GI32565903, Length=311, Percent_Identity=54.3408360128617, Blast_Score=332, Evalue=2e-91,
Organism=Caenorhabditis elegans, GI32565909, Length=87, Percent_Identity=54.0229885057471, Blast_Score=106, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI32565907, Length=44, Percent_Identity=68.1818181818182, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323209, Length=387, Percent_Identity=54.7803617571059, Blast_Score=425, Evalue=1e-120,
Organism=Saccharomyces cerevisiae, GI6320710, Length=388, Percent_Identity=54.639175257732, Blast_Score=425, Evalue=1e-120,
Organism=Drosophila melanogaster, GI45552159, Length=388, Percent_Identity=56.4432989690722, Blast_Score=408, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24580529, Length=388, Percent_Identity=56.4432989690722, Blast_Score=408, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24580537, Length=388, Percent_Identity=56.4432989690722, Blast_Score=408, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24580533, Length=388, Percent_Identity=56.4432989690722, Blast_Score=408, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24580535, Length=388, Percent_Identity=56.4432989690722, Blast_Score=408, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24580531, Length=388, Percent_Identity=56.4432989690722, Blast_Score=408, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24580527, Length=388, Percent_Identity=55.4123711340206, Blast_Score=401, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580525, Length=388, Percent_Identity=55.4123711340206, Blast_Score=401, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580541, Length=376, Percent_Identity=54.2553191489362, Blast_Score=379, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580539, Length=127, Percent_Identity=55.9055118110236, Blast_Score=137, Evalue=1e-32,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): METK_OCEIH (Q8EP05)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_693235.1
- ProteinModelPortal:   Q8EP05
- SMR:   Q8EP05
- GeneID:   1017324
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB2314
- NMPDR:   fig|221109.1.peg.2311
- HOGENOM:   HBG443662
- OMA:   QDEHARV
- ProtClustDB:   PRK05250
- BioCyc:   OIHE221109:OB2314-MONOMER
- BRENDA:   2.5.1.6
- GO:   GO:0005737
- HAMAP:   MF_00086
- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636
- PANTHER:   PTHR11964
- PIRSF:   PIRSF000497
- TIGRFAMs:   TIGR01034

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N; SSF55973 S-AdoMet_synt

EC number: =2.5.1.6

Molecular weight: Translated: 43557; Mature: 43426

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS00376 ADOMET_SYNTHASE_1; PS00377 ADOMET_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAANRRLFTSESVTEGHPDKMSDQISDAILDEILKKDPNARVACETTVTTGLVLVSGEIS
CCCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEHEEEEEEEECCCC
TSTYVDIPALVRETVKEIGYTRAKYGFDFETCAVLTAIDEQSADIAGGVNQALEARQGKM
CCEEECCHHHHHHHHHHHCCHHHHCCCCHHHHHHHHHHCCCCCHHHCCHHHHHHHHHCCC
SEEEIDAIGAGDQGLMFGYACDETEELMPLPISLAHQLSKRLADVRKEGIVDYLRPDGKT
CHHHHHHCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHCCCCCE
QVTVEYDENDKPERIDTIVISTQHHSEIQNEQIERDMIEKVIQAVVPSELLDDKTKYFIN
EEEEEECCCCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCEEEEC
PTGRFVIGGPQGDVGLTGRKIIVDTYGGYARHGGGAFSGKDATKVDRSAAYAARYVAKNI
CCCCEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
VAAGLAKSCEVQLAYAIGVAQPVSIAVNTFGTGKVSEDRLVEAVRELFDLRPAGIIRMLD
HHHHCCCCCCEEEEEEECCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHH
LRKPIYKNTAAYGHFGRKDILFPWEKTDKTEELIALTK
HCCCHHHCCCCCCCCCCCCCCCCCCCCCCHHHHEEECC
>Mature Secondary Structure 
AANRRLFTSESVTEGHPDKMSDQISDAILDEILKKDPNARVACETTVTTGLVLVSGEIS
CCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEHEEEEEEEECCCC
TSTYVDIPALVRETVKEIGYTRAKYGFDFETCAVLTAIDEQSADIAGGVNQALEARQGKM
CCEEECCHHHHHHHHHHHCCHHHHCCCCHHHHHHHHHHCCCCCHHHCCHHHHHHHHHCCC
SEEEIDAIGAGDQGLMFGYACDETEELMPLPISLAHQLSKRLADVRKEGIVDYLRPDGKT
CHHHHHHCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHCCCCCE
QVTVEYDENDKPERIDTIVISTQHHSEIQNEQIERDMIEKVIQAVVPSELLDDKTKYFIN
EEEEEECCCCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCEEEEC
PTGRFVIGGPQGDVGLTGRKIIVDTYGGYARHGGGAFSGKDATKVDRSAAYAARYVAKNI
CCCCEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
VAAGLAKSCEVQLAYAIGVAQPVSIAVNTFGTGKVSEDRLVEAVRELFDLRPAGIIRMLD
HHHHCCCCCCEEEEEEECCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHH
LRKPIYKNTAAYGHFGRKDILFPWEKTDKTEELIALTK
HCCCHHHCCCCCCCCCCCCCCCCCCCCCCHHHHEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12235376