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The map label for this gene is ilvB [H]

Identifier: 23099736

GI number: 23099736

Start: 2322883

End: 2324553

Strand: Reverse

Name: ilvB [H]

Synonym: OB2281

Alternate gene names: 23099736

Gene position: 2324553-2322883 (Counterclockwise)

Preceding gene: 23099737

Following gene: 23099735

Centisome position: 64.03

GC content: 35.85

Gene sequence:

>1671_bases
TTGAATCAAAATGGAATTGATTTAAATGCGGCAGAAGCTATTGTAAAAGGATTACGAAGAGAAGAAGTAAAGCATATTTT
TTGTGTTCCGGGTGAAAGTTATCTTCCTGTATTAAACGCACTTTATGATGAGCAAGATATACAAGTCATTTCAGGTCGTC
ATGAAGGTGGTATTGCATTTATGGCAGAAGGATATGCAAAAGCTACAAAAAAACCGGGAATTGTTATGGCAACAAGAGGA
GTGGGAGCAAGTAACCTATCCATTGGTGTACATACCGCTCATCAAGACTCTACTCCAATGGTTGTACTGTTAGGGCAAGT
AAGTACAAAGTTTAGAGGGAGAGAAGGCTTTCAAGAAGTGGATTTAGAAAGTTTCTTTCGTCCAATATCTAAATGGGCGG
TAGAAATAACCGATGTACTGCGCATACCTGAAATACTAGCTAAAGCTTTTCGAGTTGCAAAAACCGGTAGACCAGGACCA
GTAGTAATCTCTCTACCGGAAGATATCCTTTACGAACAAGGAGAATTTACTTTTAGAGCACCTGTGAGTATTCCACGTCC
GGCACCATCGATAAAAGAAATATCCATGTTTCAGCATTTTTTACAAATTGCAAAAAAACCATTGATTATCGCTGGAGGAG
GTATTCGGTTTTCTGGTGCTGAAGATGCGCTTCTTGAATTTGCCGAATCATTTCAAATACCTGTGATGACAGCCTTTCGG
AGGCATGATGTATTTCCAAACAATAATGATTTATATCTCGGACATTTAGGATTAGGTATTACATCCGAAATACGTGAAGC
TGTAGATGAAGCAGATGTTATCTTTGCATTTGGTACAAGATTATCGGAAATTACGACACAGGATTATTCGATTATTACAG
ATGAAAAACGACTTGTCCATATTGATATATCTTTTGAAACGATAGGAAATATATATTCACCAGATTTAGGCATTGTCGCA
GATATCAAAGAAGCTTTATTGGCATTAAAGACGATTAATATTGACGAGGTAAAGGGTTCAGGAGACTTTATTTGGAAAGA
ATGGGCAGAGAATAGATACACTAAACAAAAAGAATCGATAAAACAATTATATCAAAAACAGGATATTCAAAGTAAAATAA
TAGGTTATTTACAACAACAGTTAAACCATGAATTTATTATTACAAATGATGCGGGTAATTTTGCAGGTTGGCTACATACG
TATTTCCAGTTTAACCATAAAAACTCTTATATTGGACCGACATCAGGAGCAATGGGGTATGGAGTACCTTCTGCAATTGG
AGCTGCTATAGCAAGTCCACATAAAACGGTAATAGCATTATCTGGAGATGGCGGATTCATGATGACTGCACAAGAGTTAG
AAACAGCAGTAAGATATAAGGTTGGTATGTTAAGTCTTGTTTTTAATAACCAGATGTATGGAACAATTCGAATGCATCAA
GAAATACATTATCCAAAAAAAGTAATAGCAACAGACTTAGGAAATATTTCATTTAAATCGTATATTCAAAGTCTTGGAGG
TAAAGGGTATCAAGTTACCAGCTTTCAAGAATTTGAACATGTCTATCCTATAGCACTCGAACATGCAAAACATGCACCTG
TTGTCATTGAAATACTTACAGATCGCGAACAAATTAGTGTAAGTAAAAAAATAAATGACTTTCATACCTAA

Upstream 100 bases:

>100_bases
CAAGGTGAAGGATTCTTTAGAATTGCATTAACAGTACCAGAAGAAAGGTTGATTGAAGCAGTTCAACGTATTGATTCTTG
GTTGAATGGAGGAAAGTGAT

Downstream 100 bases:

>100_bases
ATTAGAATATGAAGTTGCTACAGGTATTAGCAATATTTGGTAATCAAGAATACAGTCCTTGTTATTCTTGATTTTTTTAT
GGTTATAATTTCGAATATAC

Product: thiamine pyrophosphate protein

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 556; Mature: 556

Protein sequence:

>556_residues
MNQNGIDLNAAEAIVKGLRREEVKHIFCVPGESYLPVLNALYDEQDIQVISGRHEGGIAFMAEGYAKATKKPGIVMATRG
VGASNLSIGVHTAHQDSTPMVVLLGQVSTKFRGREGFQEVDLESFFRPISKWAVEITDVLRIPEILAKAFRVAKTGRPGP
VVISLPEDILYEQGEFTFRAPVSIPRPAPSIKEISMFQHFLQIAKKPLIIAGGGIRFSGAEDALLEFAESFQIPVMTAFR
RHDVFPNNNDLYLGHLGLGITSEIREAVDEADVIFAFGTRLSEITTQDYSIITDEKRLVHIDISFETIGNIYSPDLGIVA
DIKEALLALKTINIDEVKGSGDFIWKEWAENRYTKQKESIKQLYQKQDIQSKIIGYLQQQLNHEFIITNDAGNFAGWLHT
YFQFNHKNSYIGPTSGAMGYGVPSAIGAAIASPHKTVIALSGDGGFMMTAQELETAVRYKVGMLSLVFNNQMYGTIRMHQ
EIHYPKKVIATDLGNISFKSYIQSLGGKGYQVTSFQEFEHVYPIALEHAKHAPVVIEILTDREQISVSKKINDFHT

Sequences:

>Translated_556_residues
MNQNGIDLNAAEAIVKGLRREEVKHIFCVPGESYLPVLNALYDEQDIQVISGRHEGGIAFMAEGYAKATKKPGIVMATRG
VGASNLSIGVHTAHQDSTPMVVLLGQVSTKFRGREGFQEVDLESFFRPISKWAVEITDVLRIPEILAKAFRVAKTGRPGP
VVISLPEDILYEQGEFTFRAPVSIPRPAPSIKEISMFQHFLQIAKKPLIIAGGGIRFSGAEDALLEFAESFQIPVMTAFR
RHDVFPNNNDLYLGHLGLGITSEIREAVDEADVIFAFGTRLSEITTQDYSIITDEKRLVHIDISFETIGNIYSPDLGIVA
DIKEALLALKTINIDEVKGSGDFIWKEWAENRYTKQKESIKQLYQKQDIQSKIIGYLQQQLNHEFIITNDAGNFAGWLHT
YFQFNHKNSYIGPTSGAMGYGVPSAIGAAIASPHKTVIALSGDGGFMMTAQELETAVRYKVGMLSLVFNNQMYGTIRMHQ
EIHYPKKVIATDLGNISFKSYIQSLGGKGYQVTSFQEFEHVYPIALEHAKHAPVVIEILTDREQISVSKKINDFHT
>Mature_556_residues
MNQNGIDLNAAEAIVKGLRREEVKHIFCVPGESYLPVLNALYDEQDIQVISGRHEGGIAFMAEGYAKATKKPGIVMATRG
VGASNLSIGVHTAHQDSTPMVVLLGQVSTKFRGREGFQEVDLESFFRPISKWAVEITDVLRIPEILAKAFRVAKTGRPGP
VVISLPEDILYEQGEFTFRAPVSIPRPAPSIKEISMFQHFLQIAKKPLIIAGGGIRFSGAEDALLEFAESFQIPVMTAFR
RHDVFPNNNDLYLGHLGLGITSEIREAVDEADVIFAFGTRLSEITTQDYSIITDEKRLVHIDISFETIGNIYSPDLGIVA
DIKEALLALKTINIDEVKGSGDFIWKEWAENRYTKQKESIKQLYQKQDIQSKIIGYLQQQLNHEFIITNDAGNFAGWLHT
YFQFNHKNSYIGPTSGAMGYGVPSAIGAAIASPHKTVIALSGDGGFMMTAQELETAVRYKVGMLSLVFNNQMYGTIRMHQ
EIHYPKKVIATDLGNISFKSYIQSLGGKGYQVTSFQEFEHVYPIALEHAKHAPVVIEILTDREQISVSKKINDFHT

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=579, Percent_Identity=22.9706390328152, Blast_Score=144, Evalue=3e-34,
Organism=Homo sapiens, GI21361361, Length=595, Percent_Identity=24.7058823529412, Blast_Score=139, Evalue=5e-33,
Organism=Escherichia coli, GI87081685, Length=545, Percent_Identity=30.8256880733945, Blast_Score=268, Evalue=7e-73,
Organism=Escherichia coli, GI1790104, Length=504, Percent_Identity=30.7539682539683, Blast_Score=233, Evalue=2e-62,
Organism=Escherichia coli, GI1786717, Length=570, Percent_Identity=27.3684210526316, Blast_Score=228, Evalue=6e-61,
Organism=Escherichia coli, GI1787096, Length=552, Percent_Identity=26.0869565217391, Blast_Score=152, Evalue=7e-38,
Organism=Escherichia coli, GI1788716, Length=549, Percent_Identity=23.8615664845173, Blast_Score=125, Evalue=8e-30,
Organism=Caenorhabditis elegans, GI17542570, Length=552, Percent_Identity=22.463768115942, Blast_Score=127, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17531299, Length=572, Percent_Identity=25.1748251748252, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17531301, Length=575, Percent_Identity=25.0434782608696, Blast_Score=114, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6323755, Length=576, Percent_Identity=28.125, Blast_Score=223, Evalue=4e-59,
Organism=Saccharomyces cerevisiae, GI6320816, Length=532, Percent_Identity=23.8721804511278, Blast_Score=96, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6321524, Length=495, Percent_Identity=23.6363636363636, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI19922626, Length=541, Percent_Identity=25.1386321626617, Blast_Score=137, Evalue=2e-32,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 61827; Mature: 61827

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQNGIDLNAAEAIVKGLRREEVKHIFCVPGESYLPVLNALYDEQDIQVISGRHEGGIAF
CCCCCCCCCHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHCCCCEEEEECCCCCCEEE
MAEGYAKATKKPGIVMATRGVGASNLSIGVHTAHQDSTPMVVLLGQVSTKFRGREGFQEV
EECCHHHHCCCCCEEEEECCCCCCCCEEEEEECCCCCCCEEEEEECCCHHHCCCCCCHHC
DLESFFRPISKWAVEITDVLRIPEILAKAFRVAKTGRPGPVVISLPEDILYEQGEFTFRA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCHHHHHCCCCEEEEC
PVSIPRPAPSIKEISMFQHFLQIAKKPLIIAGGGIRFSGAEDALLEFAESFQIPVMTAFR
CCCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCEEECCCHHHHHHHHHHCCCHHHHHHH
RHDVFPNNNDLYLGHLGLGITSEIREAVDEADVIFAFGTRLSEITTQDYSIITDEKRLVH
HCCCCCCCCCEEEEEECCCCHHHHHHHHCCCCEEEEECCHHHHHCCCCCEEEECCCEEEE
IDISFETIGNIYSPDLGIVADIKEALLALKTINIDEVKGSGDFIWKEWAENRYTKQKESI
EEEEHHHHCCCCCCCCCHHHHHHHHHHHHHCCCHHHCCCCCCCHHHHHHHHHHHHHHHHH
KQLYQKQDIQSKIIGYLQQQLNHEFIITNDAGNFAGWLHTYFQFNHKNSYIGPTSGAMGY
HHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHEEECCCCCCCCCCCCCCCC
GVPSAIGAAIASPHKTVIALSGDGGFMMTAQELETAVRYKVGMLSLVFNNQMYGTIRMHQ
CCHHHHHHHHCCCCEEEEEEECCCCEEEEHHHHHHHHHHHHHHEEEEECCCEEEEEEEHH
EIHYPKKVIATDLGNISFKSYIQSLGGKGYQVTSFQEFEHVYPIALEHAKHAPVVIEILT
HHCCCHHHHHHCCCCCHHHHHHHHHCCCCEEECCHHHHHHHCHHHHHCCCCCCEEEEEEC
DREQISVSKKINDFHT
CCHHHHHHHHHHCCCC
>Mature Secondary Structure
MNQNGIDLNAAEAIVKGLRREEVKHIFCVPGESYLPVLNALYDEQDIQVISGRHEGGIAF
CCCCCCCCCHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHCCCCEEEEECCCCCCEEE
MAEGYAKATKKPGIVMATRGVGASNLSIGVHTAHQDSTPMVVLLGQVSTKFRGREGFQEV
EECCHHHHCCCCCEEEEECCCCCCCCEEEEEECCCCCCCEEEEEECCCHHHCCCCCCHHC
DLESFFRPISKWAVEITDVLRIPEILAKAFRVAKTGRPGPVVISLPEDILYEQGEFTFRA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCHHHHHCCCCEEEEC
PVSIPRPAPSIKEISMFQHFLQIAKKPLIIAGGGIRFSGAEDALLEFAESFQIPVMTAFR
CCCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCEEECCCHHHHHHHHHHCCCHHHHHHH
RHDVFPNNNDLYLGHLGLGITSEIREAVDEADVIFAFGTRLSEITTQDYSIITDEKRLVH
HCCCCCCCCCEEEEEECCCCHHHHHHHHCCCCEEEEECCHHHHHCCCCCEEEECCCEEEE
IDISFETIGNIYSPDLGIVADIKEALLALKTINIDEVKGSGDFIWKEWAENRYTKQKESI
EEEEHHHHCCCCCCCCCHHHHHHHHHHHHHCCCHHHCCCCCCCHHHHHHHHHHHHHHHHH
KQLYQKQDIQSKIIGYLQQQLNHEFIITNDAGNFAGWLHTYFQFNHKNSYIGPTSGAMGY
HHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHEEECCCCCCCCCCCCCCCC
GVPSAIGAAIASPHKTVIALSGDGGFMMTAQELETAVRYKVGMLSLVFNNQMYGTIRMHQ
CCHHHHHHHHCCCCEEEEEEECCCCEEEEHHHHHHHHHHHHHHEEEEECCCEEEEEEEHH
EIHYPKKVIATDLGNISFKSYIQSLGGKGYQVTSFQEFEHVYPIALEHAKHAPVVIEILT
HHCCCHHHHHHCCCCCHHHHHHHHHCCCCEEECCHHHHHHHCHHHHHCCCCCCEEEEEEC
DREQISVSKKINDFHT
CCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]