Click here to switch to the map view.

The map label for this gene is greA

Identifier: 23099457

GI number: 23099457

Start: 2026527

End: 2027003

Strand: Reverse

Name: greA

Synonym: OB2002

Alternate gene names: 23099457

Gene position: 2027003-2026527 (Counterclockwise)

Preceding gene: 23099458

Following gene: 23099456

Centisome position: 55.83

GC content: 35.64

Gene sequence:

>477_bases
ATGACAGTTGAAAAAAGTTATTACATGACACAAGAGGGAAAAGATAAATTAGAGGAAGAATTACATTATTTAAAAACAGA
TCGTAGACAAGAAGTCGTAGAGCGTATAAAAGTTGCTCGTGACTTTGGCGATCTATCGGAGAATTCGGAGTATGATGCTG
CGAAAGATGAACAGGCATTTGTAGAGCAACGTATTACGCAAGTTGAAAAAATGATTCGTAATGCTGTAATTATAGAAAAT
GATAATGATAATCCTAATATTGTATCACTTGGTAAATCCGTTTCTTTTGTGGAGTTACCTGATGGTGATGAAGAAACCTA
TACAATTGTAGGAAGTGCTGAGGCAGATCCGTTTGAAGGAAAAATATCCAACGATTCCCCAATGGCAACAAGCTTATTAG
GAAAAGAAATTGGTGAAGAAGTAACCGTAACAACTCCAGGTGGAGATATAGATGTTCGAATTACAAATGTAGAGTAA

Upstream 100 bases:

>100_bases
TAATAAATCTGCCATAGTAGTCTTTAGTATATTGTAAATGTTTCCAGCATAAGTTGTAAGACTTATTTATTTGGAAAGTG
AAAAGAAGGAGTGTAGAGAG

Downstream 100 bases:

>100_bases
GTTTAAATGCCTGTAGAGTGACATACTTTATAGGCATTTCATTTTTGAAGTAAAACCAATTAAAAATGAATAATGTAACC
TGAAAATCCCTGTTAGAAAC

Product: transcriptional elongation factor

Products: NA

Alternate protein names: Transcript cleavage factor greA

Number of amino acids: Translated: 158; Mature: 157

Protein sequence:

>158_residues
MTVEKSYYMTQEGKDKLEEELHYLKTDRRQEVVERIKVARDFGDLSENSEYDAAKDEQAFVEQRITQVEKMIRNAVIIEN
DNDNPNIVSLGKSVSFVELPDGDEETYTIVGSAEADPFEGKISNDSPMATSLLGKEIGEEVTVTTPGGDIDVRITNVE

Sequences:

>Translated_158_residues
MTVEKSYYMTQEGKDKLEEELHYLKTDRRQEVVERIKVARDFGDLSENSEYDAAKDEQAFVEQRITQVEKMIRNAVIIEN
DNDNPNIVSLGKSVSFVELPDGDEETYTIVGSAEADPFEGKISNDSPMATSLLGKEIGEEVTVTTPGGDIDVRITNVE
>Mature_157_residues
TVEKSYYMTQEGKDKLEEELHYLKTDRRQEVVERIKVARDFGDLSENSEYDAAKDEQAFVEQRITQVEKMIRNAVIIEND
NDNPNIVSLGKSVSFVELPDGDEETYTIVGSAEADPFEGKISNDSPMATSLLGKEIGEEVTVTTPGGDIDVRITNVE

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family

Homologues:

Organism=Escherichia coli, GI87082225, Length=151, Percent_Identity=42.3841059602649, Blast_Score=114, Evalue=3e-27,
Organism=Escherichia coli, GI87082261, Length=150, Percent_Identity=34, Blast_Score=84, Evalue=5e-18,

Paralogues:

None

Copy number: 259 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): GREA_OCEIH (Q8EPT6)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_692923.1
- ProteinModelPortal:   Q8EPT6
- SMR:   Q8EPT6
- GeneID:   1018483
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB2002
- NMPDR:   fig|221109.1.peg.2001
- HOGENOM:   HBG753387
- OMA:   HNEGRIA
- BioCyc:   OIHE221109:OB2002-MONOMER
- GO:   GO:0006350
- HAMAP:   MF_00105
- InterPro:   IPR018151
- InterPro:   IPR006359
- InterPro:   IPR001437
- InterPro:   IPR022691
- Gene3D:   G3DSA:1.10.287.180
- Gene3D:   G3DSA:3.10.50.30
- TIGRFAMs:   TIGR01462

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N; SSF46557 GreA_GreB

EC number: NA

Molecular weight: Translated: 17710; Mature: 17578

Theoretical pI: Translated: 4.04; Mature: 4.04

Prosite motif: PS00829 GREAB_1; PS00830 GREAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVEKSYYMTQEGKDKLEEELHYLKTDRRQEVVERIKVARDFGDLSENSEYDAAKDEQAF
CCCCCCEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHH
VEQRITQVEKMIRNAVIIENDNDNPNIVSLGKSVSFVELPDGDEETYTIVGSAEADPFEG
HHHHHHHHHHHHHCEEEEECCCCCCCEEEECCCEEEEECCCCCCCEEEEEECCCCCCCCC
KISNDSPMATSLLGKEIGEEVTVTTPGGDIDVRITNVE
CCCCCCCHHHHHHHHHHCCEEEEECCCCCEEEEEECCC
>Mature Secondary Structure 
TVEKSYYMTQEGKDKLEEELHYLKTDRRQEVVERIKVARDFGDLSENSEYDAAKDEQAF
CCCCCEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHH
VEQRITQVEKMIRNAVIIENDNDNPNIVSLGKSVSFVELPDGDEETYTIVGSAEADPFEG
HHHHHHHHHHHHHCEEEEECCCCCCCEEEECCCEEEEECCCCCCCEEEEEECCCCCCCCC
KISNDSPMATSLLGKEIGEEVTVTTPGGDIDVRITNVE
CCCCCCCHHHHHHHHHHCCEEEEECCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12235376