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The map label for this gene is glyS

Identifier: 23099403

GI number: 23099403

Start: 1974842

End: 1976920

Strand: Reverse

Name: glyS

Synonym: OB1948

Alternate gene names: 23099403

Gene position: 1976920-1974842 (Counterclockwise)

Preceding gene: 23099404

Following gene: 23099402

Centisome position: 54.45

GC content: 35.35

Gene sequence:

>2079_bases
ATGGCACGTGATGTATTAATTGAGATTGGATTAGAAGAGCTACCTGCGCGATTTATTGATGATGCAGAGTTACAATTATA
TACCAAAACAAAGCAGTGGTTAGAAGAAAATAGAATTTCTTCAGAGAACGTAATATCTTATTCAACCCCAAGAAGATTAG
CTGTTTTTGTAAAAAATATGGCTGAAAAACAATCATCCATTGAAGAAGATGTAAAAGGTCCCGCACTTAAAATTGCCAAA
GATGAAGATGGTAATTGGACTAAGGCTGCACAAGGATTTACGAAAGGTCAGGGATTGACTACAGACGATATTGTTGTTAG
AGAAGTAAAGGGCATTTCTTATATTTATGTTACAAAACATATAGAAGGAAGACCTATTCAAGAATTATTACCTGAGTTCA
AATCGATAATTGAGTCCATAACTTTTGGTAAAAATATGCGCTGGGGTAGTGAGACTATTCGTTATGCACGACCAATCCGT
TGGTTAGTGGCTATGATGGGAAATGAAGTAATTCCATTTGAAATTGCTCATGTGGCTACAAATAATGTAACGTATGGCCA
TCGGTTTTTAGGAGAAGAAGTAACCATACAGGAACCGGCTGAATATGAGAAGACATTAAAAGATAATTATGTGATTGTAA
AAGCAAAAGATAGAGAAACGCTAATTGTTGAACAACTATCAAAACTGGAACGAGAGCATGGTTTTGAAATTCCAGTGGAT
CAGGAATTACTTGAAGAGGTCCGGAACTTAGTAGAATTCCCAACCGCATTTGTTGGAAAATTTGAAGAAGCTTACTTAGA
AATTCCACCTGAGGTTTTAATAACGTCGATGAAAGAACATCAACGATATTTTCCAGTCCACAAAAAAGGGGTCTTACAAC
CTTATTTTGTAGGTGTTCGTAATGGCGATAATTATCATATTGAAACAGTTGCCAAAGGTAATGAAAAGGTATTAAGAGCT
CGGTTAGCTGATGCAGAATTTTTCTATAACGAAGACCTTCATCAATCCATTGATTTCTTCCAAGAGAAGCTAACGAAAGT
AGTTTTTCAAGAAAAGCTAGGTACTTATTCAGATAAAGTAGAAAGAATGAAACAAATTGCTGATCGTATATCAGAGAAAC
TTTCTTTGGAATCGGACGATAGAAAAATAATTGAACGAGCAGCAGAAATTTCTAAGTTTGATTTAATGACAAGTATGGTT
AATGAGTTTACTGAATTGCAGGGGATTATTGGTGAAAAGTACGCAAATCATTTCGGCGAAAATTCAGCCACCTCACAAGC
TATAAAAGAGCATTATCAGCCCAAACATGCCAAGGATGATTTACCACAAACGGTAATCGGTTCTGTTGTTAGTGTAGCAG
ATAAGTTAGATACAATTGCTGGTTGTATAGCAGTTGGACTAGTGCCAACTGGTTCACAAGATCCATATGGACTACGGAGA
CAGGCTTCGGCTATATTACGGATATTGCATAATGAAAAATGGAATCTTACTGTAGAGGAATTAATTGATATTGCACTAGA
TGTTTTTCAAAAATCCAATGTTACTATTATGGAGAAAACCAACGAAGAATTAATTGAGTTCTTCCGTTTGCGGGCAGTGT
ATTTAATGAAGGATAAAGGCTTAGAAGTGGACGTTATCCACGCTGTAACAGATCAAAAGCTTGGTAATGTATTTGTATCA
TTCGAAAAGGCAAAGGAATTATCTGACAAACGAAATGATGAGACATTCAAACCAATTCAGGAAGCACTCGTACGTGTGTT
AAATTTATCAAATAAAGTAGAAACGAATGAATTGATTCGTGAAGATAAGTTAGAAACCGAATCTGAGAAAATTCTTTATA
CACGCTATTTGGATATCAAGGAAACTTATGAAAAACAACTCTTGCATAATGAAACAAAACAGGCATTAGCTACGCTTGCA
CAGCTTGCAGCTCCAATTCATGCGTTCTTTGACAATAATATGGTAATGGCAGATGATTTAGAGATACGTAATAACCGGCT
TGCATTAATACAAGCATTAACATCACTAATTCTTCCATATGCTGATTTAAGAAAAATTGAATGGAAACAACAGTTTTAA

Upstream 100 bases:

>100_bases
TCGAATTCGTAATTTAGCGAGAAGTATTTCCAAAATATATGTTTCCGAACGCGAAAAATTAGGTTTTCCAATGCTTCAAG
AGAAAGGAGCTAAATAAAAT

Downstream 100 bases:

>100_bases
GTTGAAAAATAGATGCTTGTTTGTACTATAACTGATACAATTAATGATGGATAAAGGTCGCAATCAAAATTAAATAGCAA
CGAATATATTGCTATTTGGG

Product: glycine-tRNA ligase beta chain

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS

Number of amino acids: Translated: 692; Mature: 691

Protein sequence:

>692_residues
MARDVLIEIGLEELPARFIDDAELQLYTKTKQWLEENRISSENVISYSTPRRLAVFVKNMAEKQSSIEEDVKGPALKIAK
DEDGNWTKAAQGFTKGQGLTTDDIVVREVKGISYIYVTKHIEGRPIQELLPEFKSIIESITFGKNMRWGSETIRYARPIR
WLVAMMGNEVIPFEIAHVATNNVTYGHRFLGEEVTIQEPAEYEKTLKDNYVIVKAKDRETLIVEQLSKLEREHGFEIPVD
QELLEEVRNLVEFPTAFVGKFEEAYLEIPPEVLITSMKEHQRYFPVHKKGVLQPYFVGVRNGDNYHIETVAKGNEKVLRA
RLADAEFFYNEDLHQSIDFFQEKLTKVVFQEKLGTYSDKVERMKQIADRISEKLSLESDDRKIIERAAEISKFDLMTSMV
NEFTELQGIIGEKYANHFGENSATSQAIKEHYQPKHAKDDLPQTVIGSVVSVADKLDTIAGCIAVGLVPTGSQDPYGLRR
QASAILRILHNEKWNLTVEELIDIALDVFQKSNVTIMEKTNEELIEFFRLRAVYLMKDKGLEVDVIHAVTDQKLGNVFVS
FEKAKELSDKRNDETFKPIQEALVRVLNLSNKVETNELIREDKLETESEKILYTRYLDIKETYEKQLLHNETKQALATLA
QLAAPIHAFFDNNMVMADDLEIRNNRLALIQALTSLILPYADLRKIEWKQQF

Sequences:

>Translated_692_residues
MARDVLIEIGLEELPARFIDDAELQLYTKTKQWLEENRISSENVISYSTPRRLAVFVKNMAEKQSSIEEDVKGPALKIAK
DEDGNWTKAAQGFTKGQGLTTDDIVVREVKGISYIYVTKHIEGRPIQELLPEFKSIIESITFGKNMRWGSETIRYARPIR
WLVAMMGNEVIPFEIAHVATNNVTYGHRFLGEEVTIQEPAEYEKTLKDNYVIVKAKDRETLIVEQLSKLEREHGFEIPVD
QELLEEVRNLVEFPTAFVGKFEEAYLEIPPEVLITSMKEHQRYFPVHKKGVLQPYFVGVRNGDNYHIETVAKGNEKVLRA
RLADAEFFYNEDLHQSIDFFQEKLTKVVFQEKLGTYSDKVERMKQIADRISEKLSLESDDRKIIERAAEISKFDLMTSMV
NEFTELQGIIGEKYANHFGENSATSQAIKEHYQPKHAKDDLPQTVIGSVVSVADKLDTIAGCIAVGLVPTGSQDPYGLRR
QASAILRILHNEKWNLTVEELIDIALDVFQKSNVTIMEKTNEELIEFFRLRAVYLMKDKGLEVDVIHAVTDQKLGNVFVS
FEKAKELSDKRNDETFKPIQEALVRVLNLSNKVETNELIREDKLETESEKILYTRYLDIKETYEKQLLHNETKQALATLA
QLAAPIHAFFDNNMVMADDLEIRNNRLALIQALTSLILPYADLRKIEWKQQF
>Mature_691_residues
ARDVLIEIGLEELPARFIDDAELQLYTKTKQWLEENRISSENVISYSTPRRLAVFVKNMAEKQSSIEEDVKGPALKIAKD
EDGNWTKAAQGFTKGQGLTTDDIVVREVKGISYIYVTKHIEGRPIQELLPEFKSIIESITFGKNMRWGSETIRYARPIRW
LVAMMGNEVIPFEIAHVATNNVTYGHRFLGEEVTIQEPAEYEKTLKDNYVIVKAKDRETLIVEQLSKLEREHGFEIPVDQ
ELLEEVRNLVEFPTAFVGKFEEAYLEIPPEVLITSMKEHQRYFPVHKKGVLQPYFVGVRNGDNYHIETVAKGNEKVLRAR
LADAEFFYNEDLHQSIDFFQEKLTKVVFQEKLGTYSDKVERMKQIADRISEKLSLESDDRKIIERAAEISKFDLMTSMVN
EFTELQGIIGEKYANHFGENSATSQAIKEHYQPKHAKDDLPQTVIGSVVSVADKLDTIAGCIAVGLVPTGSQDPYGLRRQ
ASAILRILHNEKWNLTVEELIDIALDVFQKSNVTIMEKTNEELIEFFRLRAVYLMKDKGLEVDVIHAVTDQKLGNVFVSF
EKAKELSDKRNDETFKPIQEALVRVLNLSNKVETNELIREDKLETESEKILYTRYLDIKETYEKQLLHNETKQALATLAQ
LAAPIHAFFDNNMVMADDLEIRNNRLALIQALTSLILPYADLRKIEWKQQF

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Escherichia coli, GI1789982, Length=688, Percent_Identity=34.156976744186, Blast_Score=382, Evalue=1e-107,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYGB_OCEIH (Q8EPY2)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_692869.1
- ProteinModelPortal:   Q8EPY2
- GeneID:   1018425
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB1948
- NMPDR:   fig|221109.1.peg.1948
- HOGENOM:   HBG586599
- OMA:   FIRPVHT
- BioCyc:   OIHE221109:OB1948-MONOMER
- BRENDA:   6.1.1.14
- GO:   GO:0005737
- HAMAP:   MF_00255
- InterPro:   IPR008909
- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311
- InterPro:   IPR009080
- PRINTS:   PR01045
- SMART:   SM00836
- TIGRFAMs:   TIGR00211

Pfam domain/function: PF05746 DALR_1; PF02092 tRNA_synt_2f; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.14

Molecular weight: Translated: 79559; Mature: 79428

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARDVLIEIGLEELPARFIDDAELQLYTKTKQWLEENRISSENVISYSTPRRLAVFVKNM
CCCCEEEECCHHHHCHHHCCCCHHEEHHHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHH
AEKQSSIEEDVKGPALKIAKDEDGNWTKAAQGFTKGQGLTTDDIVVREVKGISYIYVTKH
HHHHHHHHHHCCCCEEEEEECCCCCHHHHHCCCCCCCCCCHHHHHHHHHCCCEEEEEECC
IEGRPIQELLPEFKSIIESITFGKNMRWGSETIRYARPIRWLVAMMGNEVIPFEIAHVAT
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEHEE
NNVTYGHRFLGEEVTIQEPAEYEKTLKDNYVIVKAKDRETLIVEQLSKLEREHGFEIPVD
CCCCCCHHHCCCCCCCCCCHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCCC
QELLEEVRNLVEFPTAFVGKFEEAYLEIPPEVLITSMKEHQRYFPVHKKGVLQPYFVGVR
HHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHCCCCCCEEEEEE
NGDNYHIETVAKGNEKVLRARLADAEFFYNEDLHQSIDFFQEKLTKVVFQEKLGTYSDKV
CCCCEEEEEEECCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
ERMKQIADRISEKLSLESDDRKIIERAAEISKFDLMTSMVNEFTELQGIIGEKYANHFGE
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
NSATSQAIKEHYQPKHAKDDLPQTVIGSVVSVADKLDTIAGCIAVGLVPTGSQDPYGLRR
CCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHH
QASAILRILHNEKWNLTVEELIDIALDVFQKSNVTIMEKTNEELIEFFRLRAVYLMKDKG
HHHHHHHHHHCCCCCEEHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHEEECCC
LEVDVIHAVTDQKLGNVFVSFEKAKELSDKRNDETFKPIQEALVRVLNLSNKVETNELIR
CCEEEEHHHHHHHHHHHHEEHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHH
EDKLETESEKILYTRYLDIKETYEKQLLHNETKQALATLAQLAAPIHAFFDNNMVMADDL
HHHCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCC
EIRNNRLALIQALTSLILPYADLRKIEWKQQF
HHCCCHHHHHHHHHHHHCCHHHHHHHHHHCCC
>Mature Secondary Structure 
ARDVLIEIGLEELPARFIDDAELQLYTKTKQWLEENRISSENVISYSTPRRLAVFVKNM
CCCEEEECCHHHHCHHHCCCCHHEEHHHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHH
AEKQSSIEEDVKGPALKIAKDEDGNWTKAAQGFTKGQGLTTDDIVVREVKGISYIYVTKH
HHHHHHHHHHCCCCEEEEEECCCCCHHHHHCCCCCCCCCCHHHHHHHHHCCCEEEEEECC
IEGRPIQELLPEFKSIIESITFGKNMRWGSETIRYARPIRWLVAMMGNEVIPFEIAHVAT
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEHEE
NNVTYGHRFLGEEVTIQEPAEYEKTLKDNYVIVKAKDRETLIVEQLSKLEREHGFEIPVD
CCCCCCHHHCCCCCCCCCCHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCCC
QELLEEVRNLVEFPTAFVGKFEEAYLEIPPEVLITSMKEHQRYFPVHKKGVLQPYFVGVR
HHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHCCCCCCEEEEEE
NGDNYHIETVAKGNEKVLRARLADAEFFYNEDLHQSIDFFQEKLTKVVFQEKLGTYSDKV
CCCCEEEEEEECCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
ERMKQIADRISEKLSLESDDRKIIERAAEISKFDLMTSMVNEFTELQGIIGEKYANHFGE
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
NSATSQAIKEHYQPKHAKDDLPQTVIGSVVSVADKLDTIAGCIAVGLVPTGSQDPYGLRR
CCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHH
QASAILRILHNEKWNLTVEELIDIALDVFQKSNVTIMEKTNEELIEFFRLRAVYLMKDKG
HHHHHHHHHHCCCCCEEHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHEEECCC
LEVDVIHAVTDQKLGNVFVSFEKAKELSDKRNDETFKPIQEALVRVLNLSNKVETNELIR
CCEEEEHHHHHHHHHHHHEEHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHH
EDKLETESEKILYTRYLDIKETYEKQLLHNETKQALATLAQLAAPIHAFFDNNMVMADDL
HHHCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCC
EIRNNRLALIQALTSLILPYADLRKIEWKQQF
HHCCCHHHHHHHHHHHHCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12235376