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The map label for this gene is gcvT [H]

Identifier: 23099359

GI number: 23099359

Start: 1934947

End: 1936104

Strand: Reverse

Name: gcvT [H]

Synonym: OB1904

Alternate gene names: 23099359

Gene position: 1936104-1934947 (Counterclockwise)

Preceding gene: 23099362

Following gene: 23099358

Centisome position: 53.33

GC content: 33.85

Gene sequence:

>1158_bases
ATGCGCTTTTCTTTCATCAAACTAAAATTAGGAGTGGATGGAATGAGTGAACAAAAACGTACACCAATTTTCACAGAGTA
TGCATCTCATGGTGCGAAAACGATTGATTTTGGAGGATGGGATTTACCTGTACAATTCTCTAGTATTAAACATGAACATG
AAGTAACAAGAACCAAAGCAGGTCTTTTTGACGTATCTCATATGGGAGAAATTTCAGTGAAAGGGCCAAAAAGTGAATCT
TTTTTGCAATACGTATTAACAAATGACATTTCTAAATTGGAACCAGGTAAAGCACAATATACGATTATGTGTTATGAAGA
TGGTGGTACTGTAGATGATTTAATTGTTTACAAATTAGACGATGAAGATTATTTGTTAGTAGTAAATGCTGCAAACACGG
AAAAAGATGCAAATTGGATAAAACAGAAAAATACGTATTCAAACGATGAGATTGTTATTGAAGATGTATCTAATCAGTAT
GTTCAGCTTGCAATACAAGGACCTAAAGCTGTAGAGATTTTACAGAAATGTACAGATGAAAATGTACAAGAAATTAAATT
TTTTAGATTTAAGAACAATGTAGCTTTAAAAGGAATAGAAGCTAAGGCACTTATTTCACGCACTGGATATACCGGTGAAG
ATGGTTTCGAAATATATATCGACGCTTCCTCAGGAGTAGCATTGTGGAAATTGCTTTTAGAAAAAGGAGAAGCAAATGGA
CTTGAGCCAATAGGATTAGGGGCTCGAGATACTTTACGATTTGAAGCTAATTTAGCATTATATGGTCAGGAATTATCAAA
AGATATTTCCCCAATAGAAGCTGGATTAGGCTTCGCAGTAAAAGTTAATAAAGGACCGGATTTTATCGGGAAAGAAGTAT
TAAAGAACCAAGTGGAAAATGGGACTGATCGTAAGTTAGTAGGAATTGAAATGATTGATAAAGGTATTCCACGTCATGAA
TATGAGGTATTAAAAGATAATAAAGAAATTGGATTTATAACTTCTGGAACCCAATCTCCGACTCTAAACAAGAATGTTGG
GTTAGCTTTAATTAATATTTCGTATACAGAAATAGGCACTGAAGTTGATGTGAAAGTTCGAAAACGTATATTGAAGGCTA
AAATAGTTCCGACACCGTTTTATAAACGGGGAAGATAA

Upstream 100 bases:

>100_bases
CCACCCACGAAGCAAATATTTGTTCTTTTTTGAAGAATGAATATGCAACTTTCTGGTATAAGGACAGAGATTTCTTCACT
ATGGAGGAGTCTCTATTTTT

Downstream 100 bases:

>100_bases
GGGGGAGAACCAATGGAATTTCGTTACCTACCAATGACTAATCAAGATAAGCAGGAAATGCTTGATGCAATTGGAATTAA
ATCCACAGAAGAACTTTTTT

Product: aminomethyltransferase

Products: NA

Alternate protein names: Glycine cleavage system T protein [H]

Number of amino acids: Translated: 385; Mature: 385

Protein sequence:

>385_residues
MRFSFIKLKLGVDGMSEQKRTPIFTEYASHGAKTIDFGGWDLPVQFSSIKHEHEVTRTKAGLFDVSHMGEISVKGPKSES
FLQYVLTNDISKLEPGKAQYTIMCYEDGGTVDDLIVYKLDDEDYLLVVNAANTEKDANWIKQKNTYSNDEIVIEDVSNQY
VQLAIQGPKAVEILQKCTDENVQEIKFFRFKNNVALKGIEAKALISRTGYTGEDGFEIYIDASSGVALWKLLLEKGEANG
LEPIGLGARDTLRFEANLALYGQELSKDISPIEAGLGFAVKVNKGPDFIGKEVLKNQVENGTDRKLVGIEMIDKGIPRHE
YEVLKDNKEIGFITSGTQSPTLNKNVGLALINISYTEIGTEVDVKVRKRILKAKIVPTPFYKRGR

Sequences:

>Translated_385_residues
MRFSFIKLKLGVDGMSEQKRTPIFTEYASHGAKTIDFGGWDLPVQFSSIKHEHEVTRTKAGLFDVSHMGEISVKGPKSES
FLQYVLTNDISKLEPGKAQYTIMCYEDGGTVDDLIVYKLDDEDYLLVVNAANTEKDANWIKQKNTYSNDEIVIEDVSNQY
VQLAIQGPKAVEILQKCTDENVQEIKFFRFKNNVALKGIEAKALISRTGYTGEDGFEIYIDASSGVALWKLLLEKGEANG
LEPIGLGARDTLRFEANLALYGQELSKDISPIEAGLGFAVKVNKGPDFIGKEVLKNQVENGTDRKLVGIEMIDKGIPRHE
YEVLKDNKEIGFITSGTQSPTLNKNVGLALINISYTEIGTEVDVKVRKRILKAKIVPTPFYKRGR
>Mature_385_residues
MRFSFIKLKLGVDGMSEQKRTPIFTEYASHGAKTIDFGGWDLPVQFSSIKHEHEVTRTKAGLFDVSHMGEISVKGPKSES
FLQYVLTNDISKLEPGKAQYTIMCYEDGGTVDDLIVYKLDDEDYLLVVNAANTEKDANWIKQKNTYSNDEIVIEDVSNQY
VQLAIQGPKAVEILQKCTDENVQEIKFFRFKNNVALKGIEAKALISRTGYTGEDGFEIYIDASSGVALWKLLLEKGEANG
LEPIGLGARDTLRFEANLALYGQELSKDISPIEAGLGFAVKVNKGPDFIGKEVLKNQVENGTDRKLVGIEMIDKGIPRHE
YEVLKDNKEIGFITSGTQSPTLNKNVGLALINISYTEIGTEVDVKVRKRILKAKIVPTPFYKRGR

Specific function: The glycine cleavage system catalyzes the degradation of glycine [H]

COG id: COG0404

COG function: function code E; Glycine cleavage system T protein (aminomethyltransferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gcvT family [H]

Homologues:

Organism=Homo sapiens, GI44662838, Length=374, Percent_Identity=31.283422459893, Blast_Score=192, Evalue=3e-49,
Organism=Homo sapiens, GI257796258, Length=354, Percent_Identity=31.0734463276836, Blast_Score=183, Evalue=3e-46,
Organism=Homo sapiens, GI257796254, Length=371, Percent_Identity=28.0323450134771, Blast_Score=156, Evalue=3e-38,
Organism=Homo sapiens, GI24797151, Length=336, Percent_Identity=27.6785714285714, Blast_Score=152, Evalue=7e-37,
Organism=Homo sapiens, GI257796256, Length=311, Percent_Identity=29.5819935691318, Blast_Score=142, Evalue=5e-34,
Organism=Homo sapiens, GI197927446, Length=302, Percent_Identity=27.4834437086093, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI21361378, Length=302, Percent_Identity=27.4834437086093, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI194306651, Length=327, Percent_Identity=24.7706422018349, Blast_Score=109, Evalue=4e-24,
Organism=Escherichia coli, GI1789272, Length=379, Percent_Identity=39.0501319261214, Blast_Score=251, Evalue=6e-68,
Organism=Caenorhabditis elegans, GI17560118, Length=377, Percent_Identity=34.4827586206897, Blast_Score=204, Evalue=5e-53,
Organism=Caenorhabditis elegans, GI32563613, Length=320, Percent_Identity=26.5625, Blast_Score=118, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI71994052, Length=336, Percent_Identity=24.4047619047619, Blast_Score=112, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI71994045, Length=336, Percent_Identity=24.4047619047619, Blast_Score=112, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6320222, Length=396, Percent_Identity=35.3535353535354, Blast_Score=194, Evalue=3e-50,
Organism=Drosophila melanogaster, GI20129441, Length=388, Percent_Identity=33.5051546391753, Blast_Score=201, Evalue=1e-51,
Organism=Drosophila melanogaster, GI28571104, Length=346, Percent_Identity=25.7225433526012, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI20130091, Length=317, Percent_Identity=22.0820189274448, Blast_Score=90, Evalue=3e-18,

Paralogues:

None

Copy number: 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013977
- InterPro:   IPR006222
- InterPro:   IPR006223
- InterPro:   IPR022903 [H]

Pfam domain/function: PF01571 GCV_T; PF08669 GCV_T_C [H]

EC number: =2.1.2.10 [H]

Molecular weight: Translated: 42929; Mature: 42929

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFSFIKLKLGVDGMSEQKRTPIFTEYASHGAKTIDFGGWDLPVQFSSIKHEHEVTRTKA
CCEEEEEEEECCCCCCCCCCCCEEHHHHHCCCCEEECCCCCCEEEHHHHCHHHHHHHHHC
GLFDVSHMGEISVKGPKSESFLQYVLTNDISKLEPGKAQYTIMCYEDGGTVDDLIVYKLD
CEEECCCCCEEEECCCCCHHHHHHHHHCCHHHCCCCCCEEEEEEECCCCCCCEEEEEEEC
DEDYLLVVNAANTEKDANWIKQKNTYSNDEIVIEDVSNQYVQLAIQGPKAVEILQKCTDE
CCCEEEEEECCCCCCHHHHHHHCCCCCCCCEEEEECCCEEEEEEECCCHHHHHHHHHCCC
NVQEIKFFRFKNNVALKGIEAKALISRTGYTGEDGFEIYIDASSGVALWKLLLEKGEANG
CHHHEEEEEEECCEEEECCCHHHHHEECCCCCCCCEEEEEECCCCHHHHHHHHHCCCCCC
LEPIGLGARDTLRFEANLALYGQELSKDISPIEAGLGFAVKVNKGPDFIGKEVLKNQVEN
CCCCCCCCCCEEEEEEEEEEECHHHHCCCCHHHHCCCEEEEECCCCCHHHHHHHHHHHCC
GTDRKLVGIEMIDKGIPRHEYEVLKDNKEIGFITSGTQSPTLNKNVGLALINISYTEIGT
CCCCEEEEEEHHHCCCCCCHHHHHCCCCEEEEEECCCCCCCCCCCCCEEEEEEEHHHCCC
EVDVKVRKRILKAKIVPTPFYKRGR
EECHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MRFSFIKLKLGVDGMSEQKRTPIFTEYASHGAKTIDFGGWDLPVQFSSIKHEHEVTRTKA
CCEEEEEEEECCCCCCCCCCCCEEHHHHHCCCCEEECCCCCCEEEHHHHCHHHHHHHHHC
GLFDVSHMGEISVKGPKSESFLQYVLTNDISKLEPGKAQYTIMCYEDGGTVDDLIVYKLD
CEEECCCCCEEEECCCCCHHHHHHHHHCCHHHCCCCCCEEEEEEECCCCCCCEEEEEEEC
DEDYLLVVNAANTEKDANWIKQKNTYSNDEIVIEDVSNQYVQLAIQGPKAVEILQKCTDE
CCCEEEEEECCCCCCHHHHHHHCCCCCCCCEEEEECCCEEEEEEECCCHHHHHHHHHCCC
NVQEIKFFRFKNNVALKGIEAKALISRTGYTGEDGFEIYIDASSGVALWKLLLEKGEANG
CHHHEEEEEEECCEEEECCCHHHHHEECCCCCCCCEEEEEECCCCHHHHHHHHHCCCCCC
LEPIGLGARDTLRFEANLALYGQELSKDISPIEAGLGFAVKVNKGPDFIGKEVLKNQVEN
CCCCCCCCCCEEEEEEEEEEECHHHHCCCCHHHHCCCEEEEECCCCCHHHHHHHHHHHCC
GTDRKLVGIEMIDKGIPRHEYEVLKDNKEIGFITSGTQSPTLNKNVGLALINISYTEIGT
CCCCEEEEEEHHHCCCCCCHHHHHCCCCEEEEEECCCCCCCCCCCCCEEEEEEEHHHCCC
EVDVKVRKRILKAKIVPTPFYKRGR
EECHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12235376 [H]