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The map label for this gene is folD

Identifier: 23099335

GI number: 23099335

Start: 1914066

End: 1914923

Strand: Reverse

Name: folD

Synonym: OB1880

Alternate gene names: 23099335

Gene position: 1914923-1914066 (Counterclockwise)

Preceding gene: 23099336

Following gene: 23099334

Centisome position: 52.75

GC content: 35.66

Gene sequence:

>858_bases
ATGGCTACATTGTTAAATGGCAAAGAATTATCTGAAGAGTTAAAGCAAAAAATGAAAATAGAAGTAGATGAATTAAAAGA
AAAAGGTCTGACTCCACATTTAACAGTAATATTGGTCGGAGATAACCCTGCTTCCAAATCCTATGTAAAAGGAAAAGAAA
AAGCCTGTGCAGTTACAGGGATTTCTTCTAATTTAATTGAACTGCCTGAGAATATTAGCCAAGACGAACTGCTTCAAATT
ATTGACGAACAAAATAATGACGACAGTGTTCATGGCATATTAGTACAATTACCGTTACCAGATCAAATGGATGAACAGAA
GATTATACACGCTATTTCTCCTGCAAAAGATGTAGATGGATTTCATCCGATTAATGTCGGAAAAATGATGACAGGAGAAG
ATACGTTTATTCCTTGTACTCCATATGGTATTTTAACAATGTTGCGATCAAAAGATATCTCTCTTGAAGGGAAACATGCT
GTAATTATAGGAAGAAGTAATATTGTCGGTAAACCGATTGGATTGCTTTTACTACAAGAAAATGCAACAGTAACGTATAC
GCACTCTCGTACAAAGAATTTGCAAGAAATAACGAAGCAGGCAGATATACTAATTGTAGCGATAGGAAGAGCTCATGCAA
TTAATGCGGATTATATTAAGGAAGATGCCGTAGTAATTGATGTAGGCATTAATCGTAAGGATGATGGAAAACTTACTGGA
GATGTGGACTTTGAAAGTGCAGAACAAAAAGCATCTTACATTACACCGGTTCCACGTGGGGTAGGTCCCATGACGATAAC
GATGCTGCTAAAAAATACAATTAAAGCTGCAAAAGGATTGAATGACGTTGAAAGATAA

Upstream 100 bases:

>100_bases
AGCAATCGAACTGGCACATACATTTGGGGATGAACAATCAGGAAAATTTGTAAATGGTGTTTTATCAAAAATTATTGCAT
AAGGGAGAGAGAATTAACCA

Downstream 100 bases:

>100_bases
ATATTTGACGGTAACAGCTTTAACAAAATATGTTAAAAGGAAAATGGATTCTGATCCACATTTGCGGGATATTTGGTTAC
GTGGGGAAATATCAAACTTT

Product: methenyltetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase

Number of amino acids: Translated: 285; Mature: 284

Protein sequence:

>285_residues
MATLLNGKELSEELKQKMKIEVDELKEKGLTPHLTVILVGDNPASKSYVKGKEKACAVTGISSNLIELPENISQDELLQI
IDEQNNDDSVHGILVQLPLPDQMDEQKIIHAISPAKDVDGFHPINVGKMMTGEDTFIPCTPYGILTMLRSKDISLEGKHA
VIIGRSNIVGKPIGLLLLQENATVTYTHSRTKNLQEITKQADILIVAIGRAHAINADYIKEDAVVIDVGINRKDDGKLTG
DVDFESAEQKASYITPVPRGVGPMTITMLLKNTIKAAKGLNDVER

Sequences:

>Translated_285_residues
MATLLNGKELSEELKQKMKIEVDELKEKGLTPHLTVILVGDNPASKSYVKGKEKACAVTGISSNLIELPENISQDELLQI
IDEQNNDDSVHGILVQLPLPDQMDEQKIIHAISPAKDVDGFHPINVGKMMTGEDTFIPCTPYGILTMLRSKDISLEGKHA
VIIGRSNIVGKPIGLLLLQENATVTYTHSRTKNLQEITKQADILIVAIGRAHAINADYIKEDAVVIDVGINRKDDGKLTG
DVDFESAEQKASYITPVPRGVGPMTITMLLKNTIKAAKGLNDVER
>Mature_284_residues
ATLLNGKELSEELKQKMKIEVDELKEKGLTPHLTVILVGDNPASKSYVKGKEKACAVTGISSNLIELPENISQDELLQII
DEQNNDDSVHGILVQLPLPDQMDEQKIIHAISPAKDVDGFHPINVGKMMTGEDTFIPCTPYGILTMLRSKDISLEGKHAV
IIGRSNIVGKPIGLLLLQENATVTYTHSRTKNLQEITKQADILIVAIGRAHAINADYIKEDAVVIDVGINRKDDGKLTGD
VDFESAEQKASYITPVPRGVGPMTITMLLKNTIKAAKGLNDVER

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family

Homologues:

Organism=Homo sapiens, GI94721354, Length=293, Percent_Identity=50.8532423208191, Blast_Score=260, Evalue=1e-69,
Organism=Homo sapiens, GI222136639, Length=289, Percent_Identity=44.2906574394464, Blast_Score=248, Evalue=4e-66,
Organism=Homo sapiens, GI222418558, Length=293, Percent_Identity=46.0750853242321, Blast_Score=244, Evalue=5e-65,
Organism=Homo sapiens, GI36796743, Length=221, Percent_Identity=23.9819004524887, Blast_Score=72, Evalue=8e-13,
Organism=Escherichia coli, GI1786741, Length=282, Percent_Identity=48.5815602836879, Blast_Score=270, Evalue=6e-74,
Organism=Caenorhabditis elegans, GI17568735, Length=290, Percent_Identity=41.7241379310345, Blast_Score=210, Evalue=6e-55,
Organism=Saccharomyces cerevisiae, GI6319558, Length=285, Percent_Identity=47.3684210526316, Blast_Score=261, Evalue=7e-71,
Organism=Saccharomyces cerevisiae, GI6321643, Length=293, Percent_Identity=40.6143344709898, Blast_Score=229, Evalue=5e-61,
Organism=Saccharomyces cerevisiae, GI6322933, Length=326, Percent_Identity=25.4601226993865, Blast_Score=89, Evalue=1e-18,
Organism=Drosophila melanogaster, GI62472483, Length=287, Percent_Identity=43.205574912892, Blast_Score=240, Evalue=8e-64,
Organism=Drosophila melanogaster, GI45551871, Length=287, Percent_Identity=43.205574912892, Blast_Score=240, Evalue=8e-64,
Organism=Drosophila melanogaster, GI24645718, Length=287, Percent_Identity=43.205574912892, Blast_Score=240, Evalue=9e-64,
Organism=Drosophila melanogaster, GI17137370, Length=287, Percent_Identity=43.205574912892, Blast_Score=240, Evalue=9e-64,
Organism=Drosophila melanogaster, GI17136816, Length=294, Percent_Identity=43.8775510204082, Blast_Score=234, Evalue=6e-62,
Organism=Drosophila melanogaster, GI17136818, Length=294, Percent_Identity=43.8775510204082, Blast_Score=234, Evalue=7e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FOLD_OCEIH (Q8EQ43)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_692801.1
- HSSP:   P11586
- ProteinModelPortal:   Q8EQ43
- SMR:   Q8EQ43
- GeneID:   1018372
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB1880
- NMPDR:   fig|221109.1.peg.1880
- HOGENOM:   HBG328751
- OMA:   TYVRNKQ
- BioCyc:   OIHE221109:OB1880-MONOMER
- BRENDA:   1.5.1.5
- BRENDA:   3.5.4.9
- GO:   GO:0005488
- HAMAP:   MF_01576
- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020631
- Gene3D:   G3DSA:3.40.50.720
- PRINTS:   PR00085

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C

EC number: =1.5.1.5; =3.5.4.9

Molecular weight: Translated: 31120; Mature: 30989

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS00766 THF_DHG_CYH_1; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATLLNGKELSEELKQKMKIEVDELKEKGLTPHLTVILVGDNPASKSYVKGKEKACAVTG
CCCCCCHHHHHHHHHHHHCCCHHHHHHCCCCCEEEEEEECCCCCCCHHHCCCCCEEEEEC
ISSNLIELPENISQDELLQIIDEQNNDDSVHGILVQLPLPDQMDEQKIIHAISPAKDVDG
CCCCHHHCCCCCCHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCHHHHHHHCCCCHHCCC
FHPINVGKMMTGEDTFIPCTPYGILTMLRSKDISLEGKHAVIIGRSNIVGKPIGLLLLQE
CCCCCCCCEECCCCCEEEECCHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCEEEEEEEC
NATVTYTHSRTKNLQEITKQADILIVAIGRAHAINADYIKEDAVVIDVGINRKDDGKLTG
CCEEEEECCHHHHHHHHHHHCCEEEEEECCCEECCHHHCCCCCEEEEECCCCCCCCCEEC
DVDFESAEQKASYITPVPRGVGPMTITMLLKNTIKAAKGLNDVER
CCCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHCC
>Mature Secondary Structure 
ATLLNGKELSEELKQKMKIEVDELKEKGLTPHLTVILVGDNPASKSYVKGKEKACAVTG
CCCCCHHHHHHHHHHHHCCCHHHHHHCCCCCEEEEEEECCCCCCCHHHCCCCCEEEEEC
ISSNLIELPENISQDELLQIIDEQNNDDSVHGILVQLPLPDQMDEQKIIHAISPAKDVDG
CCCCHHHCCCCCCHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCHHHHHHHCCCCHHCCC
FHPINVGKMMTGEDTFIPCTPYGILTMLRSKDISLEGKHAVIIGRSNIVGKPIGLLLLQE
CCCCCCCCEECCCCCEEEECCHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCEEEEEEEC
NATVTYTHSRTKNLQEITKQADILIVAIGRAHAINADYIKEDAVVIDVGINRKDDGKLTG
CCEEEEECCHHHHHHHHHHHCCEEEEEECCCEECCHHHCCCCCEEEEECCCCCCCCCEEC
DVDFESAEQKASYITPVPRGVGPMTITMLLKNTIKAAKGLNDVER
CCCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12235376