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The map label for this gene is xerD

Identifier: 23099302

GI number: 23099302

Start: 1879380

End: 1880273

Strand: Reverse

Name: xerD

Synonym: OB1847

Alternate gene names: 23099302

Gene position: 1880273-1879380 (Counterclockwise)

Preceding gene: 23099303

Following gene: 23099301

Centisome position: 51.79

GC content: 33.33

Gene sequence:

>894_bases
ATGTTAGAATATGCGATTGAAGACTTTTTCCATTTTCTACGTATTGAACGTGGTTTATCTGACAATACACTAAGTTCCTA
TAAAAGGGATTTAACAAATTACTTGCAATATATGAAAGACCATGATAAAACTGCAACGTGGGATAAGATTTCTAGAACTG
ATATTATGGGATTTCTTTACATGCTAAAAGATCAAGGGAAATCAACAGCAACCATTTCTAGACATATTTCTTCTATTCGT
TCTTTTCATCAATTTTTAATTCGTGAACAAATCACATCAAATGATCCTAGTTTACACATTGAAACACCTAAAAAAGATCG
TAAACTTCCGGATATTTTATCTCAGGATGAGGTAGATCGATTATTAGAAATTAAGATGAATACGAAACTTTCCGTTCGAA
ATAAAGCGATGTTAGAATTATTATACGCTACTGGTCTACGGGTATCCGAGTTAATCTCTTTAAACGTGAGTGATTTACAC
CTAATGATGGGATTTGTGCAATGTTTTGGAAAAGGATCTAAAGAAAGAATTGTTCCACTTGGTGATACGGCTAAACATTA
TCTAGAAAAGTATATTTCTGAGGCTCGAGATAGTTTGATTAAAAAGAATAATCGTGAAGATGCCTTATTTGTTAATCAAC
ATGGGAGAAGACTTACTAGACAAGGTTTTTGGAAAATCTTAAAAGGGCTTACATTAGAAGCTGGAATTTTAAAAACAATT
ACACCTCATACACTACGCCACTCTTTTGCTACACATTTATTAGAGAATGGGGCAGATTTGCGTTTAGTGCAAGAGATGTT
AGGTCATGCAGATATATCAACAACTCAAGTGTATACACATGTCACAAAGGCTCGCTTAAAAGATATGTATCAATCTTATC
ATCCTAGAGCCTAA

Upstream 100 bases:

>100_bases
CGGTTTGATCCACCGCAAGGCCCAGCAGTTAAAGTCTTTCAAAAAAATAATGGACTATTTGATCTGCTACCTTTTTATTT
GTTAGGAGATGAGTAAAGCG

Downstream 100 bases:

>100_bases
TTTTTTTCAACTATGTCGTCTGACATCTAACATCAGTGAGAAGAAACATAGTAAAAGCATGTTTTGGGAATATACATAAC
AGGAGGTATGACAAACAATG

Product: integrase:recombinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MLEYAIEDFFHFLRIERGLSDNTLSSYKRDLTNYLQYMKDHDKTATWDKISRTDIMGFLYMLKDQGKSTATISRHISSIR
SFHQFLIREQITSNDPSLHIETPKKDRKLPDILSQDEVDRLLEIKMNTKLSVRNKAMLELLYATGLRVSELISLNVSDLH
LMMGFVQCFGKGSKERIVPLGDTAKHYLEKYISEARDSLIKKNNREDALFVNQHGRRLTRQGFWKILKGLTLEAGILKTI
TPHTLRHSFATHLLENGADLRLVQEMLGHADISTTQVYTHVTKARLKDMYQSYHPRA

Sequences:

>Translated_297_residues
MLEYAIEDFFHFLRIERGLSDNTLSSYKRDLTNYLQYMKDHDKTATWDKISRTDIMGFLYMLKDQGKSTATISRHISSIR
SFHQFLIREQITSNDPSLHIETPKKDRKLPDILSQDEVDRLLEIKMNTKLSVRNKAMLELLYATGLRVSELISLNVSDLH
LMMGFVQCFGKGSKERIVPLGDTAKHYLEKYISEARDSLIKKNNREDALFVNQHGRRLTRQGFWKILKGLTLEAGILKTI
TPHTLRHSFATHLLENGADLRLVQEMLGHADISTTQVYTHVTKARLKDMYQSYHPRA
>Mature_297_residues
MLEYAIEDFFHFLRIERGLSDNTLSSYKRDLTNYLQYMKDHDKTATWDKISRTDIMGFLYMLKDQGKSTATISRHISSIR
SFHQFLIREQITSNDPSLHIETPKKDRKLPDILSQDEVDRLLEIKMNTKLSVRNKAMLELLYATGLRVSELISLNVSDLH
LMMGFVQCFGKGSKERIVPLGDTAKHYLEKYISEARDSLIKKNNREDALFVNQHGRRLTRQGFWKILKGLTLEAGILKTI
TPHTLRHSFATHLLENGADLRLVQEMLGHADISTTQVYTHVTKARLKDMYQSYHPRA

Specific function: Site-specific tyrosine recombinase, which acts by catalyzing the cutting and rejoining of the recombining DNA molecules. The xerC-xerD complex is essential to convert dimers of the bacterial chromosome into monomers to permit their segregation at cell div

COG id: COG4974

COG function: function code L; Site-specific recombinase XerD

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'phage' integrase family. XerD subfamily

Homologues:

Organism=Escherichia coli, GI1789261, Length=294, Percent_Identity=43.8775510204082, Blast_Score=253, Evalue=7e-69,
Organism=Escherichia coli, GI1790244, Length=292, Percent_Identity=36.986301369863, Blast_Score=209, Evalue=2e-55,
Organism=Escherichia coli, GI1790768, Length=180, Percent_Identity=31.6666666666667, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1790767, Length=189, Percent_Identity=29.6296296296296, Blast_Score=75, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): XERD_OCEIH (Q7ZAM3)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_692768.1
- ProteinModelPortal:   Q7ZAM3
- SMR:   Q7ZAM3
- GeneID:   1018339
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB1847
- NMPDR:   fig|221109.1.peg.1846
- HOGENOM:   HBG727654
- OMA:   YLEYGRP
- BioCyc:   OIHE221109:OB1847-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01807
- InterPro:   IPR011010
- InterPro:   IPR013762
- InterPro:   IPR002104
- InterPro:   IPR010998
- InterPro:   IPR023109
- InterPro:   IPR004107
- InterPro:   IPR011932
- Gene3D:   G3DSA:1.10.150.130
- Gene3D:   G3DSA:1.10.443.10
- TIGRFAMs:   TIGR02225

Pfam domain/function: PF02899 Phage_integr_N; PF00589 Phage_integrase; SSF56349 DNA_brk_join_enz; SSF47823 L_intgrse_like_N

EC number: NA

Molecular weight: Translated: 34470; Mature: 34470

Theoretical pI: Translated: 9.83; Mature: 9.83

Prosite motif: NA

Important sites: ACT_SITE 147-147 ACT_SITE 171-171 ACT_SITE 243-243 ACT_SITE 246-246 ACT_SITE 269-269 ACT_SITE 278-278

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEYAIEDFFHFLRIERGLSDNTLSSYKRDLTNYLQYMKDHDKTATWDKISRTDIMGFLY
CCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
MLKDQGKSTATISRHISSIRSFHQFLIREQITSNDPSLHIETPKKDRKLPDILSQDEVDR
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHCCCCHHHCCHHHHHH
LLEIKMNTKLSVRNKAMLELLYATGLRVSELISLNVSDLHLMMGFVQCFGKGSKERIVPL
HHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCEEEC
GDTAKHYLEKYISEARDSLIKKNNREDALFVNQHGRRLTRQGFWKILKGLTLEAGILKTI
CHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHHHCCCCCCCHHHHC
TPHTLRHSFATHLLENGADLRLVQEMLGHADISTTQVYTHVTKARLKDMYQSYHPRA
CHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MLEYAIEDFFHFLRIERGLSDNTLSSYKRDLTNYLQYMKDHDKTATWDKISRTDIMGFLY
CCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
MLKDQGKSTATISRHISSIRSFHQFLIREQITSNDPSLHIETPKKDRKLPDILSQDEVDR
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHCCCCHHHCCHHHHHH
LLEIKMNTKLSVRNKAMLELLYATGLRVSELISLNVSDLHLMMGFVQCFGKGSKERIVPL
HHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCEEEC
GDTAKHYLEKYISEARDSLIKKNNREDALFVNQHGRRLTRQGFWKILKGLTLEAGILKTI
CHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHHHCCCCCCCHHHHC
TPHTLRHSFATHLLENGADLRLVQEMLGHADISTTQVYTHVTKARLKDMYQSYHPRA
CHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12235376