Click here to switch to the map view.

The map label for this gene is dacF [H]

Identifier: 23099297

GI number: 23099297

Start: 1873584

End: 1874750

Strand: Reverse

Name: dacF [H]

Synonym: OB1842

Alternate gene names: 23099297

Gene position: 1874750-1873584 (Counterclockwise)

Preceding gene: 23099299

Following gene: 23099296

Centisome position: 51.64

GC content: 33.93

Gene sequence:

>1167_bases
ATGAGAAAGATACGAAACATAATGATAATTGGGTTACTAGGTGTATTATTTTTTGGTCATACCGTATCAGCAGAAGAAAG
TAACCAGGAGGTTAATTTAATTGAAGATGCTACTTCTGGAATACTTATGGAACAAGATACTGGGACAATTCTATATGATA
AAAATGCACACGAACAACTTCCCCCAGCAAGTATGACAAAGATTATGACTTTGCTATTAATAATGGAATCCTTAGAAAAA
GGAGATATAGCAAAGGATGATGTTGTTATTGTAAGTGATAAAGCCGCTTCCATGGGAGGTTCTCAGATATTTTTGGAAGC
TGGAGAAGAAATGACAGTAAATGATTTATTAAAAGGAGTTGCAGTAGCATCAGGTAATGATGCAAGTGTTGCTTTAGCAG
AAAAAATAGCTGGTAGTGAAGAAGCGTTTGTTAATAAGATGAATGAAAAAGCAAAAGAATTGAATTTAAAAAATACATTA
TTCCAAAATACTACCGGATTGCCTGAGGAAAATCATTATAGTAGTGCTTATGATATGGCAATTATGGCAAACGAACTTTT
AAAGCACGAGTCAATTACAGAATACACATCAATCTATGAGGATTATCTTCGCCAAGGACAAGACAATGAATTTTGGCTTG
TTAATACAAATAAATTAGTTCGATTTTACAAAGGCGCGGATGGGTTAAAGACTGGTTATACGAGTGAAGCGAAATATTGC
TTAACAGCTACTGCAAAACGAGATAATATGCGTGTTGTTGCCGTAGTGATGGGAGCAAAGTCACCAAAAGAAAGAAACAG
TATGGTAACAGAATTGCTTGATTATGCATTCCACAGCTATGAAACAAATCCTTTATTCTCAAAAGGCGACAAGGTAACGG
AAGTAGATTTATTAAAGGCTGATCAATCGAAGGTAGATATCGTTACGGATCATTCTGTAAGTACGATTCACAAAAAAGGT
GACGAGAAGAAGAATATTTCTACAGAAGTAAAATTACAAGAATCTTGGACATTCCCCATTCAAAAAGGGGATGAAGTAGG
TAAATTAATCATTAAAGATGGAGATACAATTATATCTGAAGTACCATTAACGGTTAGTAAAAATATAGAAAAAGCATCAT
TTAGTGATCTAATGAAAAGATCCTTACAAGAATTGGTGAAACATTAA

Upstream 100 bases:

>100_bases
AAAGTTCATCGTTTTAGGAAGTAAGATAATTTCATCAGAGGTATGAAAACACTAATTGAAGGAAAAACTATTGCTATATA
TTGAAGTTGGAGGTAAGGCT

Downstream 100 bases:

>100_bases
TAATAAAGTAACTTTTTCACTTCTTTTGTCGACAGGCAGGAATTCGCTTAAGTCTTGAAGAAATTAGCTATCTAAGAAAT
ATTTTAAAGGGAGGAATCGT

Product: D-alanyl-D-alanine carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein dacF; PBP [H]

Number of amino acids: Translated: 388; Mature: 388

Protein sequence:

>388_residues
MRKIRNIMIIGLLGVLFFGHTVSAEESNQEVNLIEDATSGILMEQDTGTILYDKNAHEQLPPASMTKIMTLLLIMESLEK
GDIAKDDVVIVSDKAASMGGSQIFLEAGEEMTVNDLLKGVAVASGNDASVALAEKIAGSEEAFVNKMNEKAKELNLKNTL
FQNTTGLPEENHYSSAYDMAIMANELLKHESITEYTSIYEDYLRQGQDNEFWLVNTNKLVRFYKGADGLKTGYTSEAKYC
LTATAKRDNMRVVAVVMGAKSPKERNSMVTELLDYAFHSYETNPLFSKGDKVTEVDLLKADQSKVDIVTDHSVSTIHKKG
DEKKNISTEVKLQESWTFPIQKGDEVGKLIIKDGDTIISEVPLTVSKNIEKASFSDLMKRSLQELVKH

Sequences:

>Translated_388_residues
MRKIRNIMIIGLLGVLFFGHTVSAEESNQEVNLIEDATSGILMEQDTGTILYDKNAHEQLPPASMTKIMTLLLIMESLEK
GDIAKDDVVIVSDKAASMGGSQIFLEAGEEMTVNDLLKGVAVASGNDASVALAEKIAGSEEAFVNKMNEKAKELNLKNTL
FQNTTGLPEENHYSSAYDMAIMANELLKHESITEYTSIYEDYLRQGQDNEFWLVNTNKLVRFYKGADGLKTGYTSEAKYC
LTATAKRDNMRVVAVVMGAKSPKERNSMVTELLDYAFHSYETNPLFSKGDKVTEVDLLKADQSKVDIVTDHSVSTIHKKG
DEKKNISTEVKLQESWTFPIQKGDEVGKLIIKDGDTIISEVPLTVSKNIEKASFSDLMKRSLQELVKH
>Mature_388_residues
MRKIRNIMIIGLLGVLFFGHTVSAEESNQEVNLIEDATSGILMEQDTGTILYDKNAHEQLPPASMTKIMTLLLIMESLEK
GDIAKDDVVIVSDKAASMGGSQIFLEAGEEMTVNDLLKGVAVASGNDASVALAEKIAGSEEAFVNKMNEKAKELNLKNTL
FQNTTGLPEENHYSSAYDMAIMANELLKHESITEYTSIYEDYLRQGQDNEFWLVNTNKLVRFYKGADGLKTGYTSEAKYC
LTATAKRDNMRVVAVVMGAKSPKERNSMVTELLDYAFHSYETNPLFSKGDKVTEVDLLKADQSKVDIVTDHSVSTIHKKG
DEKKNISTEVKLQESWTFPIQKGDEVGKLIIKDGDTIISEVPLTVSKNIEKASFSDLMKRSLQELVKH

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Secreted (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1787062, Length=374, Percent_Identity=35.2941176470588, Blast_Score=189, Evalue=4e-49,
Organism=Escherichia coli, GI87082022, Length=354, Percent_Identity=33.0508474576271, Blast_Score=175, Evalue=4e-45,
Organism=Escherichia coli, GI1786851, Length=351, Percent_Identity=34.1880341880342, Blast_Score=167, Evalue=7e-43,
Organism=Escherichia coli, GI87082054, Length=227, Percent_Identity=28.1938325991189, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 43054; Mature: 43054

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKIRNIMIIGLLGVLFFGHTVSAEESNQEVNLIEDATSGILMEQDTGTILYDKNAHEQL
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCEEEECCCCEEEECCCCCCCC
PPASMTKIMTLLLIMESLEKGDIAKDDVVIVSDKAASMGGSQIFLEAGEEMTVNDLLKGV
CCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCEEEEECCCCCCHHHHHHHH
AVASGNDASVALAEKIAGSEEAFVNKMNEKAKELNLKNTLFQNTTGLPEENHYSSAYDMA
EEECCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCHHHHHH
IMANELLKHESITEYTSIYEDYLRQGQDNEFWLVNTNKLVRFYKGADGLKTGYTSEAKYC
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECHHHEEEECCCCCCCCCCCCCCCEE
LTATAKRDNMRVVAVVMGAKSPKERNSMVTELLDYAFHSYETNPLFSKGDKVTEVDLLKA
EEEECCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEC
DQSKVDIVTDHSVSTIHKKGDEKKNISTEVKLQESWTFPIQKGDEVGKLIIKDGDTIISE
CCCCEEEEECCCHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCCCCEEEEECCCCEEHH
VPLTVSKNIEKASFSDLMKRSLQELVKH
CCCHHHCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRKIRNIMIIGLLGVLFFGHTVSAEESNQEVNLIEDATSGILMEQDTGTILYDKNAHEQL
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCEEEECCCCEEEECCCCCCCC
PPASMTKIMTLLLIMESLEKGDIAKDDVVIVSDKAASMGGSQIFLEAGEEMTVNDLLKGV
CCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCEEEEECCCCCCHHHHHHHH
AVASGNDASVALAEKIAGSEEAFVNKMNEKAKELNLKNTLFQNTTGLPEENHYSSAYDMA
EEECCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCHHHHHH
IMANELLKHESITEYTSIYEDYLRQGQDNEFWLVNTNKLVRFYKGADGLKTGYTSEAKYC
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECHHHEEEECCCCCCCCCCCCCCCEE
LTATAKRDNMRVVAVVMGAKSPKERNSMVTELLDYAFHSYETNPLFSKGDKVTEVDLLKA
EEEECCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEC
DQSKVDIVTDHSVSTIHKKGDEKKNISTEVKLQESWTFPIQKGDEVGKLIIKDGDTIISE
CCCCEEEEECCCHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCCCCEEEEECCCCEEHH
VPLTVSKNIEKASFSDLMKRSLQELVKH
CCCHHHCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1629150; 8969508; 9384377 [H]