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The map label for this gene is spoVAF [H]

Identifier: 23099289

GI number: 23099289

Start: 1867613

End: 1869088

Strand: Reverse

Name: spoVAF [H]

Synonym: OB1834

Alternate gene names: 23099289

Gene position: 1869088-1867613 (Counterclockwise)

Preceding gene: 23099290

Following gene: 23099288

Centisome position: 51.48

GC content: 34.42

Gene sequence:

>1476_bases
ATGAATAGACTAAAATCAGAGCAAAAGCGTATGATTTCACCGAAAATTGAAGAGAATTTCAATTATTTGAAGACAAAACT
TGGAATTAAAACTTCCTTTGATATAGGTTATCGAGAACTTATTATTCTGAAGCAACGTATTCATATTTATTATGTAACTG
GACTTTGTGATACATCAGTTATTCAAGAAATGTTAAAAAAACTAGTAGAAATTAATGATGTAGAAACAAATAAAAGAATG
CTTCCAGAAATAATTGAAAATAGACTTGTGCATCAACAAGTTGAGTCCGTTAAAACAATGGATGAAGCAGTTGATCAAGT
TCTAAGTGGTCTTGTTGCCGTATTTATAGACGGAGAGCGATATGCTCAAATAGTTGATGTACGTAATTATCCAGGCAGAT
CTCCAGAAGAACCTGATACAGAAAAAGTTATTCGAGGATCAAGGGACGGTTATACTGAAAATATCATTGAAAATACAGCA
CTTACCCGGAGAAGGCTACGGGATGAAAATTTAAGAAATGAAATGGTAAAAGTCGGAGAGAGGTCGAAAACAGATGTCTG
TATCACTTATCTACAAGATGTAGCTGATGATGGGCTAATTAAACTTATTAAAAACCATATTAGTCAAATTAAACTTGATG
GGATATCCATGGCAGAAAAAACATTAGAAGAGTTTTTAATTTTACGAAGATGGAATATATACCCGATGGTTCGTTATACA
GAACGTCCAGATGTAGCTGCAAGTCATCTATTAGAAGGACATGTACTGATTTTTGTAGATACATCACCTAGTGCAATTAT
ACTGCCTACCACTTACTTTCACCATCTTCAACACGCTGAAGAATATCGACAAGTACCTTCTATTGGTACGTTTATAAGAA
TAATTCGATTTATGGCAGTATTAGCAGCGTTATTTTTGTTGCCAACATGGGTATTGTTTGCTAACGAACCGTCCTTATTA
CCAGAAAGCTTAAGCTTTATTGGTCCTAACGATGAAGGAAATGTACCTATTGTTATACAAGCACTCTTAGCAATGATAGG
TATTGAATTTTTACGGATGGCGGCCATTCACACCCCTACACCACTATCAACGTCAATGGGATTAATTGCAGCAGTCTTAA
TTGGAGAGATAGCGATTGAAGTAGGGTTATTTTCACCTGAAGTTATACTCTATGTATCTACAGCTTCGATAGGTAATTAT
GTCACGCCAAGTTATGAAATAAGTGTAGCAAATAAAGTTGTTAGTATGACTCTTGTGATTATTGTTGGATTATTTGGCCT
CCCAGGTTATATGATATCCATTACTTTGTATATCCTTTTCCTGATTCAAATAAAGTCATTGAAAACACCATATTTATGGC
CGTTAATTCCATTTAATTCGAAAGCAATGGTTCAAATTCTTATTCGTGTTCCAGTTCCTTATACGAAGAGTAGACCAAGT
ATTGTCCATCCTAAAAATAATTACCGTCAACCATAA

Upstream 100 bases:

>100_bases
ATTAATTTTTATGATGGCACTATTACTAGGTAAAGTGTTCGGATCCTTATTTCAATGGACCATATTTGTACGATAAACAA
AAAGGCAGGTTATCGATTTT

Downstream 100 bases:

>100_bases
CTTAGTAGGAGCTTGTTCAAATGCTTTTTCGAACAAGCTCTTACTTTTTAAGCATCTTGATTTCAACTCACACTTTTGTT
TTTTACAGTGTTGTGATAAA

Product: stage V sporulation protein AF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 491; Mature: 491

Protein sequence:

>491_residues
MNRLKSEQKRMISPKIEENFNYLKTKLGIKTSFDIGYRELIILKQRIHIYYVTGLCDTSVIQEMLKKLVEINDVETNKRM
LPEIIENRLVHQQVESVKTMDEAVDQVLSGLVAVFIDGERYAQIVDVRNYPGRSPEEPDTEKVIRGSRDGYTENIIENTA
LTRRRLRDENLRNEMVKVGERSKTDVCITYLQDVADDGLIKLIKNHISQIKLDGISMAEKTLEEFLILRRWNIYPMVRYT
ERPDVAASHLLEGHVLIFVDTSPSAIILPTTYFHHLQHAEEYRQVPSIGTFIRIIRFMAVLAALFLLPTWVLFANEPSLL
PESLSFIGPNDEGNVPIVIQALLAMIGIEFLRMAAIHTPTPLSTSMGLIAAVLIGEIAIEVGLFSPEVILYVSTASIGNY
VTPSYEISVANKVVSMTLVIIVGLFGLPGYMISITLYILFLIQIKSLKTPYLWPLIPFNSKAMVQILIRVPVPYTKSRPS
IVHPKNNYRQP

Sequences:

>Translated_491_residues
MNRLKSEQKRMISPKIEENFNYLKTKLGIKTSFDIGYRELIILKQRIHIYYVTGLCDTSVIQEMLKKLVEINDVETNKRM
LPEIIENRLVHQQVESVKTMDEAVDQVLSGLVAVFIDGERYAQIVDVRNYPGRSPEEPDTEKVIRGSRDGYTENIIENTA
LTRRRLRDENLRNEMVKVGERSKTDVCITYLQDVADDGLIKLIKNHISQIKLDGISMAEKTLEEFLILRRWNIYPMVRYT
ERPDVAASHLLEGHVLIFVDTSPSAIILPTTYFHHLQHAEEYRQVPSIGTFIRIIRFMAVLAALFLLPTWVLFANEPSLL
PESLSFIGPNDEGNVPIVIQALLAMIGIEFLRMAAIHTPTPLSTSMGLIAAVLIGEIAIEVGLFSPEVILYVSTASIGNY
VTPSYEISVANKVVSMTLVIIVGLFGLPGYMISITLYILFLIQIKSLKTPYLWPLIPFNSKAMVQILIRVPVPYTKSRPS
IVHPKNNYRQP
>Mature_491_residues
MNRLKSEQKRMISPKIEENFNYLKTKLGIKTSFDIGYRELIILKQRIHIYYVTGLCDTSVIQEMLKKLVEINDVETNKRM
LPEIIENRLVHQQVESVKTMDEAVDQVLSGLVAVFIDGERYAQIVDVRNYPGRSPEEPDTEKVIRGSRDGYTENIIENTA
LTRRRLRDENLRNEMVKVGERSKTDVCITYLQDVADDGLIKLIKNHISQIKLDGISMAEKTLEEFLILRRWNIYPMVRYT
ERPDVAASHLLEGHVLIFVDTSPSAIILPTTYFHHLQHAEEYRQVPSIGTFIRIIRFMAVLAALFLLPTWVLFANEPSLL
PESLSFIGPNDEGNVPIVIQALLAMIGIEFLRMAAIHTPTPLSTSMGLIAAVLIGEIAIEVGLFSPEVILYVSTASIGNY
VTPSYEISVANKVVSMTLVIIVGLFGLPGYMISITLYILFLIQIKSLKTPYLWPLIPFNSKAMVQILIRVPVPYTKSRPS
IVHPKNNYRQP

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gerABKA family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004995 [H]

Pfam domain/function: PF03323 GerA [H]

EC number: NA

Molecular weight: Translated: 55766; Mature: 55766

Theoretical pI: Translated: 7.36; Mature: 7.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRLKSEQKRMISPKIEENFNYLKTKLGIKTSFDIGYRELIILKQRIHIYYVTGLCDTSV
CCCCHHHHHHHCCCCHHHHHHHHHHHHCCCEECCCCHHHHHHEEEEEEEEEEECCCCHHH
IQEMLKKLVEINDVETNKRMLPEIIENRLVHQQVESVKTMDEAVDQVLSGLVAVFIDGER
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCH
YAQIVDVRNYPGRSPEEPDTEKVIRGSRDGYTENIIENTALTRRRLRDENLRNEMVKVGE
HEEEEECCCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
RSKTDVCITYLQDVADDGLIKLIKNHISQIKLDGISMAEKTLEEFLILRRWNIYPMVRYT
CCCCHHHHHHHHHHCCCHHHHHHHHHHHHEEECCHHHHHHHHHHHHHHHHCCCCEEEEEC
ERPDVAASHLLEGHVLIFVDTSPSAIILPTTYFHHLQHAEEYRQVPSIGTFIRIIRFMAV
CCCCHHHHHHHCCCEEEEEECCCCEEEECHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
LAALFLLPTWVLFANEPSLLPESLSFIGPNDEGNVPIVIQALLAMIGIEFLRMAAIHTPT
HHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
PLSTSMGLIAAVLIGEIAIEVGLFSPEVILYVSTASIGNYVTPSYEISVANKVVSMTLVI
CCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCEEEHHHHHHHHHHHHH
IVGLFGLPGYMISITLYILFLIQIKSLKTPYLWPLIPFNSKAMVQILIRVPVPYTKSRPS
HHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHEEEECCCCCCCCCCC
IVHPKNNYRQP
EECCCCCCCCC
>Mature Secondary Structure
MNRLKSEQKRMISPKIEENFNYLKTKLGIKTSFDIGYRELIILKQRIHIYYVTGLCDTSV
CCCCHHHHHHHCCCCHHHHHHHHHHHHCCCEECCCCHHHHHHEEEEEEEEEEECCCCHHH
IQEMLKKLVEINDVETNKRMLPEIIENRLVHQQVESVKTMDEAVDQVLSGLVAVFIDGER
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCH
YAQIVDVRNYPGRSPEEPDTEKVIRGSRDGYTENIIENTALTRRRLRDENLRNEMVKVGE
HEEEEECCCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
RSKTDVCITYLQDVADDGLIKLIKNHISQIKLDGISMAEKTLEEFLILRRWNIYPMVRYT
CCCCHHHHHHHHHHCCCHHHHHHHHHHHHEEECCHHHHHHHHHHHHHHHHCCCCEEEEEC
ERPDVAASHLLEGHVLIFVDTSPSAIILPTTYFHHLQHAEEYRQVPSIGTFIRIIRFMAV
CCCCHHHHHHHCCCEEEEEECCCCEEEECHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
LAALFLLPTWVLFANEPSLLPESLSFIGPNDEGNVPIVIQALLAMIGIEFLRMAAIHTPT
HHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
PLSTSMGLIAAVLIGEIAIEVGLFSPEVILYVSTASIGNYVTPSYEISVANKVVSMTLVI
CCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCEEEHHHHHHHHHHHHH
IVGLFGLPGYMISITLYILFLIQIKSLKTPYLWPLIPFNSKAMVQILIRVPVPYTKSRPS
HHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHEEEECCCCCCCCCCC
IVHPKNNYRQP
EECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377; 7934829; 1368705; 3926949 [H]