Click here to switch to the map view.

The map label for this gene is sleB

Identifier: 23099261

GI number: 23099261

Start: 1842343

End: 1843173

Strand: Reverse

Name: sleB

Synonym: OB1806

Alternate gene names: 23099261

Gene position: 1843173-1842343 (Counterclockwise)

Preceding gene: 23099262

Following gene: 23099260

Centisome position: 50.77

GC content: 38.03

Gene sequence:

>831_bases
ATGCATCAATCGTATAAGGCATTCATGATAATACTTGTTGTTTGTAGTAGTCTAACTATGTATTTTACACAGACAGACAA
CACAAAAACATATGCATTTAGTGATCAAGTCATCCAACAAGGTGCGGTTGGAGAAGATGTTATTGAGCTACAAGCTAGAT
TACAGTATTTAGGTTTTTACAACGGCAAAATTGATGGAGTCTTTGGTTGGGGTACCTATTGGGCATTACGCAATTTCCAG
TATGAATTTGGCATGGAAATAGATGGATTGGCAGGTCAAACAACAAAAGATAAATTAGTTCAAGCAAGTAATTACGATAA
AGAATATGTTACAGAGCAAATAAACAAAGGAAATCAGTTTACGCACTATGGTGGCGGAGATAGGAACACAGATGGTCAAA
GTAATTCAGGTGGTGGCAGTGGCAATAGTGGTGAAGACACTGGTGGTACTCCTTCAGTAAATGTTCCACAAGGTTATTCT
CAAAATGATATTCAATTAATGGCAAATGCAGTATATGGAGAGGCAAGAGGTGAACCATACGAAGGTCAGGTTGCTGTTGC
GGCCGTTATACTTAACCGTTTAAATAGTGCATTGTTCCCGGATACAGTATCAGGTGTCATTTTTGAACCACGTGCATTTA
CTGCAGTAGCTGATGGCCAAATCTGGTTAGAACCAAATGACAAAGCTAGAGAAGCAGTTTTAGATGCAATTAATGGTTGG
GATCCTTCAGGAAATGCCCTTTACTATTTTAATCCTGATACTGCAACTTCTGGCTGGATTTGGACGAGACCACAAATTAA
ACAAATTGGTAAACATATATTTTGTAAATAG

Upstream 100 bases:

>100_bases
ACTGTTTGATAGTTTTTTTATTTCTTATCTGAATGAATAAAAAGGAAAATAACACAAAAAATAGTGGTGCATCCTATTAC
AAGGAACGGAGGAAATAAAA

Downstream 100 bases:

>100_bases
GAAGGTGTGAGAATTATGTTTCGCTGGATCGCAATAGGTGTACTTAGTGTAGCTGTAATTGGAACAGCGGTATGGGGTTA
TAATGAATATAGTGAAAAGA

Product: sporulation specific N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: SCLE

Number of amino acids: Translated: 276; Mature: 276

Protein sequence:

>276_residues
MHQSYKAFMIILVVCSSLTMYFTQTDNTKTYAFSDQVIQQGAVGEDVIELQARLQYLGFYNGKIDGVFGWGTYWALRNFQ
YEFGMEIDGLAGQTTKDKLVQASNYDKEYVTEQINKGNQFTHYGGGDRNTDGQSNSGGGSGNSGEDTGGTPSVNVPQGYS
QNDIQLMANAVYGEARGEPYEGQVAVAAVILNRLNSALFPDTVSGVIFEPRAFTAVADGQIWLEPNDKAREAVLDAINGW
DPSGNALYYFNPDTATSGWIWTRPQIKQIGKHIFCK

Sequences:

>Translated_276_residues
MHQSYKAFMIILVVCSSLTMYFTQTDNTKTYAFSDQVIQQGAVGEDVIELQARLQYLGFYNGKIDGVFGWGTYWALRNFQ
YEFGMEIDGLAGQTTKDKLVQASNYDKEYVTEQINKGNQFTHYGGGDRNTDGQSNSGGGSGNSGEDTGGTPSVNVPQGYS
QNDIQLMANAVYGEARGEPYEGQVAVAAVILNRLNSALFPDTVSGVIFEPRAFTAVADGQIWLEPNDKAREAVLDAINGW
DPSGNALYYFNPDTATSGWIWTRPQIKQIGKHIFCK
>Mature_276_residues
MHQSYKAFMIILVVCSSLTMYFTQTDNTKTYAFSDQVIQQGAVGEDVIELQARLQYLGFYNGKIDGVFGWGTYWALRNFQ
YEFGMEIDGLAGQTTKDKLVQASNYDKEYVTEQINKGNQFTHYGGGDRNTDGQSNSGGGSGNSGEDTGGTPSVNVPQGYS
QNDIQLMANAVYGEARGEPYEGQVAVAAVILNRLNSALFPDTVSGVIFEPRAFTAVADGQIWLEPNDKAREAVLDAINGW
DPSGNALYYFNPDTATSGWIWTRPQIKQIGKHIFCK

Specific function: Required for spore cortex hydrolysis during germination

COG id: COG3773

COG function: function code M; Cell wall hydrolyses involved in spore germination

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sleB family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SLEB_OCEIH (P59105)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_692727.1
- ProteinModelPortal:   P59105
- SMR:   P59105
- GeneID:   1018300
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB1806
- NMPDR:   fig|221109.1.peg.1807
- HOGENOM:   HBG498645
- OMA:   YNGKIDG
- BioCyc:   OIHE221109:OB1806-MONOMER
- InterPro:   IPR011105
- InterPro:   IPR002477
- InterPro:   IPR014224
- Gene3D:   G3DSA:1.10.101.10
- TIGRFAMs:   TIGR02869

Pfam domain/function: PF07486 Hydrolase_2; PF01471 PG_binding_1; SSF47090 PGBD_like

EC number: 3.5.1.28

Molecular weight: Translated: 30363; Mature: 30363

Theoretical pI: Translated: 4.44; Mature: 4.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHQSYKAFMIILVVCSSLTMYFTQTDNTKTYAFSDQVIQQGAVGEDVIELQARLQYLGFY
CCCHHHHHHHHHHHHCCCEEEEEECCCCEEEEEHHHHHHCCCCCHHHHHHHHHHHHHHEE
NGKIDGVFGWGTYWALRNFQYEFGMEIDGLAGQTTKDKLVQASNYDKEYVTEQINKGNQF
CCEECEEECCHHHHHHHCCCCCCCCEECCCCCCCHHHHHHHCCCCCHHHHHHHHCCCCCE
THYGGGDRNTDGQSNSGGGSGNSGEDTGGTPSVNVPQGYSQNDIQLMANAVYGEARGEPY
EECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCC
EGQVAVAAVILNRLNSALFPDTVSGVIFEPRAFTAVADGQIWLEPNDKAREAVLDAINGW
CCHHHHHHHHHHHHHHHCCCCCCCCEEECCCEEEEEECCEEEECCCHHHHHHHHHHHCCC
DPSGNALYYFNPDTATSGWIWTRPQIKQIGKHIFCK
CCCCCEEEEECCCCCCCCEEEECHHHHHHHHHHCCC
>Mature Secondary Structure
MHQSYKAFMIILVVCSSLTMYFTQTDNTKTYAFSDQVIQQGAVGEDVIELQARLQYLGFY
CCCHHHHHHHHHHHHCCCEEEEEECCCCEEEEEHHHHHHCCCCCHHHHHHHHHHHHHHEE
NGKIDGVFGWGTYWALRNFQYEFGMEIDGLAGQTTKDKLVQASNYDKEYVTEQINKGNQF
CCEECEEECCHHHHHHHCCCCCCCCEECCCCCCCHHHHHHHCCCCCHHHHHHHHCCCCCE
THYGGGDRNTDGQSNSGGGSGNSGEDTGGTPSVNVPQGYSQNDIQLMANAVYGEARGEPY
EECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCC
EGQVAVAAVILNRLNSALFPDTVSGVIFEPRAFTAVADGQIWLEPNDKAREAVLDAINGW
CCHHHHHHHHHHHHHHHCCCCCCCCEEECCCEEEEEECCEEEECCCHHHHHHHHHHHCCC
DPSGNALYYFNPDTATSGWIWTRPQIKQIGKHIFCK
CCCCCEEEEECCCCCCCCEEEECHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12235376