Click here to switch to the map view.

The map label for this gene is nth [H]

Identifier: 23099212

GI number: 23099212

Start: 1796385

End: 1797035

Strand: Reverse

Name: nth [H]

Synonym: OB1757

Alternate gene names: 23099212

Gene position: 1797035-1796385 (Counterclockwise)

Preceding gene: 23099213

Following gene: 23099209

Centisome position: 49.5

GC content: 35.79

Gene sequence:

>651_bases
GTGCTTAACCAAAAACAAATACGACAATGTCTAGATATTATGGCGGAGATGTACCCTGATGCTAAAGGCGAATTGGAACA
CTCTAATGCTTTTGAACTGGTTATTGCTGTTCTATTGTCTGCCCAATGCACAGATAAGTTAGTCAATAAAGTAACTGCAG
ATCTTTTCCAAAAATATAAGACACCTGAAGATTATCTTTCTGTCGAATTAAGTGAGTTAGAAAATGATATTCGTTCTATA
GGGCTATATCGTTCTAAAGCAAAAAATATACAAAAACTTTGTCAAATGTTATTAGATGAATATAATGGGGAAATTCCTTC
GTCAAAAGAGGAATTAGTTAAATTAGCAGGTGTTGGCAGAAAAACAGCAAATGTCGTAGCATCGATTGCATTTAATGAGC
CTTCTATAGCAGTAGACACACATGTAGAACGTGTATCCAAACGTCTTGGTATATGCAAATGGAAGGATAGCGTTCTAGAA
GTAGAAAATACCTTAATGAAAAAAGTACCACGTGATGAATGGAGTGTAACCCATCACCGAATGATATTCTTTGGCAGATA
TCATTGCAAAGCAAGAAATCCGCAATGTCCAGAATGCCCGCTTCTCGAATTATGTAGAGAAGGAAAGAAAAGAATGAAGA
AACTAGCATAA

Upstream 100 bases:

>100_bases
AAAAGCAAGTCAACCATTCCATCAAAATCAACAATCGCCAAAAACGAGTCAAAAGAGAGACACCTCGTTCTACTATAATT
GGCTAGAGGGGGAATAAAAT

Downstream 100 bases:

>100_bases
GTAAGAATATAAAAAGACGCAGCACGTGCTGCGTCTTTTATATTTAGTTAATTTTAATTTTCTTCACCAGAATCTCCGGT
TTCATCTTCGGGATTTCCAT

Product: endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 216; Mature: 216

Protein sequence:

>216_residues
MLNQKQIRQCLDIMAEMYPDAKGELEHSNAFELVIAVLLSAQCTDKLVNKVTADLFQKYKTPEDYLSVELSELENDIRSI
GLYRSKAKNIQKLCQMLLDEYNGEIPSSKEELVKLAGVGRKTANVVASIAFNEPSIAVDTHVERVSKRLGICKWKDSVLE
VENTLMKKVPRDEWSVTHHRMIFFGRYHCKARNPQCPECPLLELCREGKKRMKKLA

Sequences:

>Translated_216_residues
MLNQKQIRQCLDIMAEMYPDAKGELEHSNAFELVIAVLLSAQCTDKLVNKVTADLFQKYKTPEDYLSVELSELENDIRSI
GLYRSKAKNIQKLCQMLLDEYNGEIPSSKEELVKLAGVGRKTANVVASIAFNEPSIAVDTHVERVSKRLGICKWKDSVLE
VENTLMKKVPRDEWSVTHHRMIFFGRYHCKARNPQCPECPLLELCREGKKRMKKLA
>Mature_216_residues
MLNQKQIRQCLDIMAEMYPDAKGELEHSNAFELVIAVLLSAQCTDKLVNKVTADLFQKYKTPEDYLSVELSELENDIRSI
GLYRSKAKNIQKLCQMLLDEYNGEIPSSKEELVKLAGVGRKTANVVASIAFNEPSIAVDTHVERVSKRLGICKWKDSVLE
VENTLMKKVPRDEWSVTHHRMIFFGRYHCKARNPQCPECPLLELCREGKKRMKKLA

Specific function: Has Both An Apurinic And/Or Apyrimidinic Endonuclease Activity And A DNA N-Glycosylase Activity. Incises Damaged DNA At Cytosines, Thymines And Guanines. Acts On A Damaged Strand, 5' From The Damaged Site. Required For The Repair Of Both Oxidative DNA Da

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=180, Percent_Identity=31.6666666666667, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI115298648, Length=186, Percent_Identity=27.9569892473118, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI6912520, Length=186, Percent_Identity=27.9569892473118, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI190358497, Length=186, Percent_Identity=27.9569892473118, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI115298650, Length=186, Percent_Identity=27.9569892473118, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI115298654, Length=186, Percent_Identity=27.9569892473118, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI115298652, Length=186, Percent_Identity=27.9569892473118, Blast_Score=70, Evalue=1e-12,
Organism=Escherichia coli, GI1787920, Length=209, Percent_Identity=44.9760765550239, Blast_Score=168, Evalue=2e-43,
Organism=Escherichia coli, GI1789331, Length=153, Percent_Identity=28.7581699346405, Blast_Score=62, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17554540, Length=177, Percent_Identity=34.4632768361582, Blast_Score=106, Evalue=8e-24,
Organism=Saccharomyces cerevisiae, GI6319304, Length=178, Percent_Identity=26.4044943820225, Blast_Score=73, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6324530, Length=190, Percent_Identity=25.7894736842105, Blast_Score=65, Evalue=1e-11,
Organism=Drosophila melanogaster, GI45550361, Length=177, Percent_Identity=32.2033898305085, Blast_Score=101, Evalue=4e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 24743; Mature: 24743

Theoretical pI: Translated: 8.39; Mature: 8.39

Prosite motif: PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
6.9 %Cys+Met (Translated Protein)
3.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
6.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNQKQIRQCLDIMAEMYPDAKGELEHSNAFELVIAVLLSAQCTDKLVNKVTADLFQKYK
CCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
TPEDYLSVELSELENDIRSIGLYRSKAKNIQKLCQMLLDEYNGEIPSSKEELVKLAGVGR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCC
KTANVVASIAFNEPSIAVDTHVERVSKRLGICKWKDSVLEVENTLMKKVPRDEWSVTHHR
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
MIFFGRYHCKARNPQCPECPLLELCREGKKRMKKLA
HHHEEHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLNQKQIRQCLDIMAEMYPDAKGELEHSNAFELVIAVLLSAQCTDKLVNKVTADLFQKYK
CCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
TPEDYLSVELSELENDIRSIGLYRSKAKNIQKLCQMLLDEYNGEIPSSKEELVKLAGVGR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCC
KTANVVASIAFNEPSIAVDTHVERVSKRLGICKWKDSVLEVENTLMKKVPRDEWSVTHHR
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
MIFFGRYHCKARNPQCPECPLLELCREGKKRMKKLA
HHHEEHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7704260; 8760912; 9384377 [H]