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The map label for this gene is thyA

Identifier: 23099195

GI number: 23099195

Start: 1780213

End: 1781166

Strand: Reverse

Name: thyA

Synonym: OB1740

Alternate gene names: 23099195

Gene position: 1781166-1780213 (Counterclockwise)

Preceding gene: 23099197

Following gene: 23099194

Centisome position: 49.06

GC content: 34.49

Gene sequence:

>954_bases
ATGTTAGGGGAACAAGCCTACTTAGATTTGTGTAAACAAATATTTAAAACTGGAGAACAACGTGGAGATCGAACCAATAC
AGGAACACATTCTATATTCGGACATCAAATTCGTTTTGATTTAAATAAAGGATTTCCTCTTCTAACGACAAAACGTGTTC
CATTCCGACTAGTTGCGAGTGAACTACTTTGGTTTATTAAAGGGGATACCAATATTCGGTATTTATTAAAACATAATAAT
AATATTTGGAATGAATGGGCATTTGAAAAATGGGTAAATAGCGCTGATTATCAAGGTCCAGATATGGCGAATTTCGGATT
ACGCAGTCAAAAAGATGAGAATTTTAATAAACAATATCAAGAACAAATGGAGCTCTTTAAAACGCGAATTCTTGAAGATG
ATTCTTTTGCTGACAAATACGGGGACTTAGGATCTGTATACGGGAAACAATGGAGAAATTGGAAGACTACTCAGAATGAA
ACAATTGATCAATTAAAAGATGTGATTCATTCCATTAAAACGAATCCAAATTCAAGAAGACATATTGTATCTGCGTGGAA
TCCAGAAGATATTCCAACAATGGCTTTACCTCCTTGTCATACATTATTCCAATTTTATGTTTCAAATGGAAAGCTTTCTT
GTCAGCTATACCAAAGAAGTGCAGACGTATTCCTAGGGGTACCATTCAATATAGCCAGTTATGCCCTACTCACGCATTTA
ATTGCCCATGAGTGTGGATTAGAAGTTGGTGATTTCGTACATACTTTTGGAGACGCACATATTTACTCTAATCATGTTGA
ACAAGTCGAAACACAACTGGAAAGGGAGATCCGCGATTTTCCACAACTAATAATAAACAAAGATAAAAATTCTATTTTTG
AAATGGACTTAGATGATTTAGTGATTGAAAATTATAATCCTCATCCAAGTATTAAAGCACCGATTGCTGTATAG

Upstream 100 bases:

>100_bases
GTTTTACGATTATGTAACAAAAGAGGACTAGTATCTGTTATAATTAAATCATTTGAGAAGCAAAATCTATATTTTAAATA
TAGTCAGGAGAGATTAATAT

Downstream 100 bases:

>100_bases
TTCAAAATAGATACTCACACAACGAATCTAAATAGGAAGTTGAGGTTATAATATGATTTCGTTATTACTTGCTATGGATC
GCAATCATGTAATTGGAGTT

Product: thymidylate synthase

Products: NA

Alternate protein names: TS; TSase

Number of amino acids: Translated: 317; Mature: 317

Protein sequence:

>317_residues
MLGEQAYLDLCKQIFKTGEQRGDRTNTGTHSIFGHQIRFDLNKGFPLLTTKRVPFRLVASELLWFIKGDTNIRYLLKHNN
NIWNEWAFEKWVNSADYQGPDMANFGLRSQKDENFNKQYQEQMELFKTRILEDDSFADKYGDLGSVYGKQWRNWKTTQNE
TIDQLKDVIHSIKTNPNSRRHIVSAWNPEDIPTMALPPCHTLFQFYVSNGKLSCQLYQRSADVFLGVPFNIASYALLTHL
IAHECGLEVGDFVHTFGDAHIYSNHVEQVETQLEREIRDFPQLIINKDKNSIFEMDLDDLVIENYNPHPSIKAPIAV

Sequences:

>Translated_317_residues
MLGEQAYLDLCKQIFKTGEQRGDRTNTGTHSIFGHQIRFDLNKGFPLLTTKRVPFRLVASELLWFIKGDTNIRYLLKHNN
NIWNEWAFEKWVNSADYQGPDMANFGLRSQKDENFNKQYQEQMELFKTRILEDDSFADKYGDLGSVYGKQWRNWKTTQNE
TIDQLKDVIHSIKTNPNSRRHIVSAWNPEDIPTMALPPCHTLFQFYVSNGKLSCQLYQRSADVFLGVPFNIASYALLTHL
IAHECGLEVGDFVHTFGDAHIYSNHVEQVETQLEREIRDFPQLIINKDKNSIFEMDLDDLVIENYNPHPSIKAPIAV
>Mature_317_residues
MLGEQAYLDLCKQIFKTGEQRGDRTNTGTHSIFGHQIRFDLNKGFPLLTTKRVPFRLVASELLWFIKGDTNIRYLLKHNN
NIWNEWAFEKWVNSADYQGPDMANFGLRSQKDENFNKQYQEQMELFKTRILEDDSFADKYGDLGSVYGKQWRNWKTTQNE
TIDQLKDVIHSIKTNPNSRRHIVSAWNPEDIPTMALPPCHTLFQFYVSNGKLSCQLYQRSADVFLGVPFNIASYALLTHL
IAHECGLEVGDFVHTFGDAHIYSNHVEQVETQLEREIRDFPQLIINKDKNSIFEMDLDDLVIENYNPHPSIKAPIAV

Specific function: Provides the sole de novo source of dTMP for DNA biosynthesis

COG id: COG0207

COG function: function code F; Thymidylate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate synthase family. Bacterial- type thyA subfamily

Homologues:

Organism=Homo sapiens, GI4507751, Length=323, Percent_Identity=47.3684210526316, Blast_Score=284, Evalue=7e-77,
Organism=Escherichia coli, GI1789191, Length=313, Percent_Identity=49.5207667731629, Blast_Score=312, Evalue=2e-86,
Organism=Caenorhabditis elegans, GI71993377, Length=323, Percent_Identity=43.9628482972136, Blast_Score=264, Evalue=4e-71,
Organism=Saccharomyces cerevisiae, GI6324648, Length=223, Percent_Identity=49.7757847533632, Blast_Score=203, Evalue=2e-53,
Organism=Drosophila melanogaster, GI17137556, Length=324, Percent_Identity=49.6913580246914, Blast_Score=298, Evalue=2e-81,

Paralogues:

None

Copy number: 391 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): TYSY_OCEIH (Q8EQG0)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_692661.1
- ProteinModelPortal:   Q8EQG0
- SMR:   Q8EQG0
- GeneID:   1018235
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB1740
- NMPDR:   fig|221109.1.peg.1742
- HOGENOM:   HBG588098
- OMA:   RMALAPC
- ProtClustDB:   PRK01827
- BioCyc:   OIHE221109:OB1740-MONOMER
- BRENDA:   2.1.1.45
- GO:   GO:0005737
- HAMAP:   MF_00008
- InterPro:   IPR000398
- InterPro:   IPR020940
- Gene3D:   G3DSA:3.30.572.10
- PANTHER:   PTHR11549:SF2
- PRINTS:   PR00108
- TIGRFAMs:   TIGR03284

Pfam domain/function: PF00303 Thymidylat_synt; SSF55831 Thymidylat_synth_C

EC number: =2.1.1.45

Molecular weight: Translated: 36834; Mature: 36834

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: PS00091 THYMIDYLATE_SYNTHASE

Important sites: ACT_SITE 199-199

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLGEQAYLDLCKQIFKTGEQRGDRTNTGTHSIFGHQIRFDLNKGFPLLTTKRVPFRLVAS
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCEEEEECCCCCCEEEECCCHHHHHHH
ELLWFIKGDTNIRYLLKHNNNIWNEWAFEKWVNSADYQGPDMANFGLRSQKDENFNKQYQ
HHHEEEECCCCEEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
EQMELFKTRILEDDSFADKYGDLGSVYGKQWRNWKTTQNETIDQLKDVIHSIKTNPNSRR
HHHHHHHHHHCCCCCHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCC
HIVSAWNPEDIPTMALPPCHTLFQFYVSNGKLSCQLYQRSADVFLGVPFNIASYALLTHL
EEEECCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCEEEECCHHHHHHHHHHHH
IAHECGLEVGDFVHTFGDAHIYSNHVEQVETQLEREIRDFPQLIINKDKNSIFEMDLDDL
HHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCHHHH
VIENYNPHPSIKAPIAV
HHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MLGEQAYLDLCKQIFKTGEQRGDRTNTGTHSIFGHQIRFDLNKGFPLLTTKRVPFRLVAS
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCEEEEECCCCCCEEEECCCHHHHHHH
ELLWFIKGDTNIRYLLKHNNNIWNEWAFEKWVNSADYQGPDMANFGLRSQKDENFNKQYQ
HHHEEEECCCCEEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
EQMELFKTRILEDDSFADKYGDLGSVYGKQWRNWKTTQNETIDQLKDVIHSIKTNPNSRR
HHHHHHHHHHCCCCCHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCC
HIVSAWNPEDIPTMALPPCHTLFQFYVSNGKLSCQLYQRSADVFLGVPFNIASYALLTHL
EEEECCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCEEEECCHHHHHHHHHHHH
IAHECGLEVGDFVHTFGDAHIYSNHVEQVETQLEREIRDFPQLIINKDKNSIFEMDLDDL
HHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCHHHH
VIENYNPHPSIKAPIAV
HHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12235376