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The map label for this gene is dfrA [H]

Identifier: 23099194

GI number: 23099194

Start: 1779672

End: 1780160

Strand: Reverse

Name: dfrA [H]

Synonym: OB1739

Alternate gene names: 23099194

Gene position: 1780160-1779672 (Counterclockwise)

Preceding gene: 23099195

Following gene: 28563932

Centisome position: 49.03

GC content: 31.29

Gene sequence:

>489_bases
ATGATTTCGTTATTACTTGCTATGGATCGCAATCATGTAATTGGAGTTAATAATCAATTGCCTTGGCATTTGCCAAAGGA
TTTACGTTTTTTTAAAGAAAAGACAACAGGTAATACAATTATTATGGGAAGAAAAACATTTGAATCGATGGGAGGGGCAT
TACCAAATCGAAAAAATATTGTCTTAACCAGTCGTTCCGATGTACAGTATCCGAATGCTAAAATAATTCATCACATAGAC
ACGATTAAACAATGGAATATAGAAAATCCGGAAGAAGAATATTTTGTAATCGGTGGTGCAAACTTATTTCAACAAGTAAT
GGATATTGCTGATCGAATGTATATAACTTGGATTGACGAATCATTTACCGGAGATACATTCTTTCCCAAATTTAATCAAG
AAGACTGGATAATATCATCTAAAATTAAAGGTGATAAAGATGAAAAAAACCCTTATGATTATTACTTCATTCAATACGAT
CGAAAATAA

Upstream 100 bases:

>100_bases
GAAAATTATAATCCTCATCCAAGTATTAAAGCACCGATTGCTGTATAGTTCAAAATAGATACTCACACAACGAATCTAAA
TAGGAAGTTGAGGTTATAAT

Downstream 100 bases:

>100_bases
ACAAACGAAGATTTATGAATAACTTGTTTAATTATTTCTAATCGTGCTATGATATGTATAAAGTAAAGAGCTACTCAATA
ATGCATAGATAGTACTGATT

Product: dihydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 162; Mature: 162

Protein sequence:

>162_residues
MISLLLAMDRNHVIGVNNQLPWHLPKDLRFFKEKTTGNTIIMGRKTFESMGGALPNRKNIVLTSRSDVQYPNAKIIHHID
TIKQWNIENPEEEYFVIGGANLFQQVMDIADRMYITWIDESFTGDTFFPKFNQEDWIISSKIKGDKDEKNPYDYYFIQYD
RK

Sequences:

>Translated_162_residues
MISLLLAMDRNHVIGVNNQLPWHLPKDLRFFKEKTTGNTIIMGRKTFESMGGALPNRKNIVLTSRSDVQYPNAKIIHHID
TIKQWNIENPEEEYFVIGGANLFQQVMDIADRMYITWIDESFTGDTFFPKFNQEDWIISSKIKGDKDEKNPYDYYFIQYD
RK
>Mature_162_residues
MISLLLAMDRNHVIGVNNQLPWHLPKDLRFFKEKTTGNTIIMGRKTFESMGGALPNRKNIVLTSRSDVQYPNAKIIHHID
TIKQWNIENPEEEYFVIGGANLFQQVMDIADRMYITWIDESFTGDTFFPKFNQEDWIISSKIKGDKDEKNPYDYYFIQYD
RK

Specific function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis [H]

COG id: COG0262

COG function: function code H; Dihydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DHFR (dihydrofolate reductase) domain [H]

Homologues:

Organism=Escherichia coli, GI1786233, Length=162, Percent_Identity=37.037037037037, Blast_Score=110, Evalue=5e-26,
Organism=Drosophila melanogaster, GI116008026, Length=141, Percent_Identity=31.2056737588652, Blast_Score=68, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24647458, Length=141, Percent_Identity=31.2056737588652, Blast_Score=68, Evalue=2e-12,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 10,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012259
- InterPro:   IPR017925
- InterPro:   IPR001796 [H]

Pfam domain/function: PF00186 DHFR_1 [H]

EC number: =1.5.1.3 [H]

Molecular weight: Translated: 19115; Mature: 19115

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00075 DHFR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISLLLAMDRNHVIGVNNQLPWHLPKDLRFFKEKTTGNTIIMGRKTFESMGGALPNRKNI
CEEEEEEECCCCEEEECCCCCCCCCHHHHHHHHCCCCCEEEEECHHHHHHCCCCCCCCEE
VLTSRSDVQYPNAKIIHHIDTIKQWNIENPEEEYFVIGGANLFQQVMDIADRMYITWIDE
EEECCCCCCCCCCEEEEEEHHHHHCCCCCCCCCEEEEECHHHHHHHHHHHHCEEEEEECC
SFTGDTFFPKFNQEDWIISSKIKGDKDEKNPYDYYFIQYDRK
CCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCEEEEEEECCC
>Mature Secondary Structure
MISLLLAMDRNHVIGVNNQLPWHLPKDLRFFKEKTTGNTIIMGRKTFESMGGALPNRKNI
CEEEEEEECCCCEEEECCCCCCCCCHHHHHHHHCCCCCEEEEECHHHHHHCCCCCCCCEE
VLTSRSDVQYPNAKIIHHIDTIKQWNIENPEEEYFVIGGANLFQQVMDIADRMYITWIDE
EEECCCCCCCCCCEEEEEEHHHHHCCCCCCCCCEEEEECHHHHHHHHHHHHCEEEEEECC
SFTGDTFFPKFNQEDWIISSKIKGDKDEKNPYDYYFIQYDRK
CCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2840350; 9384377 [H]