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The map label for this gene is rasP

Identifier: 23099047

GI number: 23099047

Start: 1627755

End: 1629029

Strand: Direct

Name: rasP

Synonym: OB1592

Alternate gene names: 23099047

Gene position: 1627755-1629029 (Clockwise)

Preceding gene: 23099046

Following gene: 23099048

Centisome position: 44.84

GC content: 37.1

Gene sequence:

>1275_bases
ATGACAACGGTTGTTGCATTTATACTTATGTTTGGAGTCTTAGTCTCCATTCATGAATGGGGACATTTAATTTTTGCAAA
ACGAGCAGGTATGCTCGTACGAGAATTTGCGATCGGTTTTGGCCCCAAAATTTTTTCCTTCACCAAGAACGAAACTCTGT
ATACGATTCGTTTAATACCTGCTGGTGGATACGTTCGTGTAGCTGGTGAAGATCCTGAAATTATCGAATTAAAACCTGGA
CATCATATCGGTCTTGAATTCAATCAAGCTGGTGAAGTTTCTAAAATCATTGTAAATAACAAATCAAAGCATCCTTATGC
TAGAGTGATTGAAGTAGAGAATGTAGACCTTGATCATAAATTAATTATTGAAGGTTACGAATTAGATGATGATGAAAATC
GATTATCTTTCAATGTTAATGAAAAAGCGATGTTTGTTATGGACGAGCGAGAAACACAAATTGCTCCATATAATCGACAA
TTTGCTTCGAAGTCTACAGGGAAACGTGCCATGCAATTATTTGCTGGACCAATGATGAACTTTGTATTAGCGATAGCAAT
TTTCCTTATTTTAGGAATAATTCAAGGTGTGCCAGTGGAGGAAGCCAAACTTGGTGAAATTCAGCCCGATACACCAGCTG
AACAAGCTGGTTTCCAACAAGATGATGTAATTACGCAAATAGGAGATCAGTCTATATCTACATGGGAAGAATTTACTTCT
ATTGTACGAGAAAATCCTGGACAGGAATTAGATATGGTTATTCAACGAAATGGTGAATCACAGGATATTTCTGTTGTTCC
TGGTGAAGCAGAGGCCGTTAATGAAGTTGGAGATCCTATTACGATTGGACAAATTGGTGTTTACCAGGGGTTTGAAAAAG
ATGTATTAGGAACATTTGTATATGGTATAGAAAGAACGTATGATACAACCACCATGATTATACAAAACTTATTTATGTTA
GTAACTGGGCAAGTTTCTATTGAACTTCTATCGGGACCGGTTGGCATTTATGATGCCACAGACCAAGTGGTACAGACTGG
TTTCTCAAACTTCCTATTATGGACAGCGATGTTAAGCATTAATTTAGGAATAATTAATCTCGTTCCATTACCAGCGTTGG
ATGGTGGACGTCTGCTCTTTGTTGGACTTGAAGCTGTTCGTGGAAAACCAATTGCTCCTGAAAAGGAAGGCATATTCCAT
TTTGTAGGATTTGCTTTACTTATGTTATTAATGATTGTAGTTACGTGGAATGACATACAACGATTATTCTTGTAA

Upstream 100 bases:

>100_bases
TCCGTCGAAAAGGTTAATCTTAACCTATTGGTGAATATAATTAACGGAGAGCTTGTATACTTAATTCAAATGCCTGCTCC
ACTAAAAGGAAGGTGTTATT

Downstream 100 bases:

>100_bases
AATATATAAAGAGAACTTAGAGGTTGGGACTCAATAAAAAGTATGGTTCAAAAGACGATTGGTTATTTTGAAGTAGTGCC
AGAAGTATACATTGACGCTT

Product: hypothetical protein

Products: NA

Alternate protein names: Regulating alternative sigma factor protease; Regulating anti-sigma-W factor activity protease

Number of amino acids: Translated: 424; Mature: 423

Protein sequence:

>424_residues
MTTVVAFILMFGVLVSIHEWGHLIFAKRAGMLVREFAIGFGPKIFSFTKNETLYTIRLIPAGGYVRVAGEDPEIIELKPG
HHIGLEFNQAGEVSKIIVNNKSKHPYARVIEVENVDLDHKLIIEGYELDDDENRLSFNVNEKAMFVMDERETQIAPYNRQ
FASKSTGKRAMQLFAGPMMNFVLAIAIFLILGIIQGVPVEEAKLGEIQPDTPAEQAGFQQDDVITQIGDQSISTWEEFTS
IVRENPGQELDMVIQRNGESQDISVVPGEAEAVNEVGDPITIGQIGVYQGFEKDVLGTFVYGIERTYDTTTMIIQNLFML
VTGQVSIELLSGPVGIYDATDQVVQTGFSNFLLWTAMLSINLGIINLVPLPALDGGRLLFVGLEAVRGKPIAPEKEGIFH
FVGFALLMLLMIVVTWNDIQRLFL

Sequences:

>Translated_424_residues
MTTVVAFILMFGVLVSIHEWGHLIFAKRAGMLVREFAIGFGPKIFSFTKNETLYTIRLIPAGGYVRVAGEDPEIIELKPG
HHIGLEFNQAGEVSKIIVNNKSKHPYARVIEVENVDLDHKLIIEGYELDDDENRLSFNVNEKAMFVMDERETQIAPYNRQ
FASKSTGKRAMQLFAGPMMNFVLAIAIFLILGIIQGVPVEEAKLGEIQPDTPAEQAGFQQDDVITQIGDQSISTWEEFTS
IVRENPGQELDMVIQRNGESQDISVVPGEAEAVNEVGDPITIGQIGVYQGFEKDVLGTFVYGIERTYDTTTMIIQNLFML
VTGQVSIELLSGPVGIYDATDQVVQTGFSNFLLWTAMLSINLGIINLVPLPALDGGRLLFVGLEAVRGKPIAPEKEGIFH
FVGFALLMLLMIVVTWNDIQRLFL
>Mature_423_residues
TTVVAFILMFGVLVSIHEWGHLIFAKRAGMLVREFAIGFGPKIFSFTKNETLYTIRLIPAGGYVRVAGEDPEIIELKPGH
HIGLEFNQAGEVSKIIVNNKSKHPYARVIEVENVDLDHKLIIEGYELDDDENRLSFNVNEKAMFVMDERETQIAPYNRQF
ASKSTGKRAMQLFAGPMMNFVLAIAIFLILGIIQGVPVEEAKLGEIQPDTPAEQAGFQQDDVITQIGDQSISTWEEFTSI
VRENPGQELDMVIQRNGESQDISVVPGEAEAVNEVGDPITIGQIGVYQGFEKDVLGTFVYGIERTYDTTTMIIQNLFMLV
TGQVSIELLSGPVGIYDATDQVVQTGFSNFLLWTAMLSINLGIINLVPLPALDGGRLLFVGLEAVRGKPIAPEKEGIFHF
VGFALLMLLMIVVTWNDIQRLFL

Specific function: Is responsible for Site-2 cleavage of the RsiW anti- sigma factor. This results, after a third proteolytic step catalyzed by the ClpXP protease, in the release of SigW and the transcription activation of the genes under the control of the sigma-W factor

COG id: COG0750

COG function: function code M; Predicted membrane-associated Zn-dependent proteases 1

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PDZ (DHR) domain

Homologues:

Organism=Escherichia coli, GI1786373, Length=465, Percent_Identity=30.1075268817204, Blast_Score=162, Evalue=4e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RASP_OCEIH (Q8EQU7)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_692513.1
- ProteinModelPortal:   Q8EQU7
- MEROPS:   M50.004
- GeneID:   1017980
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB1592
- NMPDR:   fig|221109.1.peg.1594
- HOGENOM:   HBG664087
- OMA:   GMIDESM
- BioCyc:   OIHE221109:OB1592-MONOMER
- GO:   GO:0006508
- InterPro:   IPR001478
- InterPro:   IPR004387
- InterPro:   IPR008915
- SMART:   SM00228
- TIGRFAMs:   TIGR00054

Pfam domain/function: PF02163 Peptidase_M50; SSF50156 PDZ

EC number: NA

Molecular weight: Translated: 47042; Mature: 46911

Theoretical pI: Translated: 4.37; Mature: 4.37

Prosite motif: PS50106 PDZ; PS00142 ZINC_PROTEASE

Important sites: ACT_SITE 19-19

Signals:

None

Transmembrane regions:

HASH(0xe81a66c)-; HASH(0xefa4a9c)-; HASH(0xe6d0ce4)-; HASH(0xefa4724)-; HASH(0xe80dca8)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTVVAFILMFGVLVSIHEWGHLIFAKRAGMLVREFAIGFGPKIFSFTKNETLYTIRLIP
CHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHCCCCCEEEEECCCEEEEEEEEE
AGGYVRVAGEDPEIIELKPGHHIGLEFNQAGEVSKIIVNNKSKHPYARVIEVENVDLDHK
CCCEEEEECCCCCEEEECCCCEEEEEECCCCCEEEEEECCCCCCCEEEEEEEECCCCCCE
LIIEGYELDDDENRLSFNVNEKAMFVMDERETQIAPYNRQFASKSTGKRAMQLFAGPMMN
EEEEEEECCCCCCEEEEEECCEEEEEEECCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHH
FVLAIAIFLILGIIQGVPVEEAKLGEIQPDTPAEQAGFQQDDVITQIGDQSISTWEEFTS
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHCCCCCCHHHHHHCCCCHHHHHHHHH
IVRENPGQELDMVIQRNGESQDISVVPGEAEAVNEVGDPITIGQIGVYQGFEKDVLGTFV
HHHCCCCCCCHHHEECCCCCCCEEEECCCHHHHHHCCCCEEECCCHHCCCCCHHHHHHHH
YGIERTYDTTTMIIQNLFMLVTGQVSIELLSGPVGIYDATDQVVQTGFSNFLLWTAMLSI
HHHHCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCEECCHHHHHHHCHHHHHHHHHHHHH
NLGIINLVPLPALDGGRLLFVGLEAVRGKPIAPEKEGIFHFVGFALLMLLMIVVTWNDIQ
CCCEEEEEECCCCCCCEEEEEEHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
RLFL
HHCH
>Mature Secondary Structure 
TTVVAFILMFGVLVSIHEWGHLIFAKRAGMLVREFAIGFGPKIFSFTKNETLYTIRLIP
HHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHCCCCCEEEEECCCEEEEEEEEE
AGGYVRVAGEDPEIIELKPGHHIGLEFNQAGEVSKIIVNNKSKHPYARVIEVENVDLDHK
CCCEEEEECCCCCEEEECCCCEEEEEECCCCCEEEEEECCCCCCCEEEEEEEECCCCCCE
LIIEGYELDDDENRLSFNVNEKAMFVMDERETQIAPYNRQFASKSTGKRAMQLFAGPMMN
EEEEEEECCCCCCEEEEEECCEEEEEEECCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHH
FVLAIAIFLILGIIQGVPVEEAKLGEIQPDTPAEQAGFQQDDVITQIGDQSISTWEEFTS
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHCCCCCCHHHHHHCCCCHHHHHHHHH
IVRENPGQELDMVIQRNGESQDISVVPGEAEAVNEVGDPITIGQIGVYQGFEKDVLGTFV
HHHCCCCCCCHHHEECCCCCCCEEEECCCHHHHHHCCCCEEECCCHHCCCCCHHHHHHHH
YGIERTYDTTTMIIQNLFMLVTGQVSIELLSGPVGIYDATDQVVQTGFSNFLLWTAMLSI
HHHHCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCEECCHHHHHHHCHHHHHHHHHHHHH
NLGIINLVPLPALDGGRLLFVGLEAVRGKPIAPEKEGIFHFVGFALLMLLMIVVTWNDIQ
CCCEEEEEECCCCCCCEEEEEEHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
RLFL
HHCH

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12235376