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The map label for this gene is rnh

Identifier: 23098995

GI number: 23098995

Start: 1582431

End: 1583216

Strand: Direct

Name: rnh

Synonym: OB1540

Alternate gene names: 23098995

Gene position: 1582431-1583216 (Clockwise)

Preceding gene: 23098994

Following gene: 23098996

Centisome position: 43.59

GC content: 35.11

Gene sequence:

>786_bases
ATGAAACCAGCTTCTATTGCAAGTATAAAATCACAACTAACCAACGGGGAACTAACTCAAAAACAAATCGAAGAATTATA
CAAAGATAGCAGAAAAGGTGTGCAAGCAATACTAAAGCGTCATGAAAAACAACAAGAAAAACAAGCGCTATTAAAACAAG
CATTCATTAAAATGAACACATATGAAAAGGAAGCTTACCGTAATGGGAATCACTATATAGCTGGTGTGGATGAAGTGGGG
CGTGGCCCTTTAGCTGGTCCGGTAGTTGCAGCAGCAGTTATTTTACCAAAGGACTTTCAACTTTTAGGTATAAATGATTC
CAAGAAATTGTCAGAAGTACAAAGAAATGAATTTAGCCATTACATCAAACAACATGCAATTGCTTATCAAATATCATTTA
TTGATAATACCATAATCGATAAAATCAATATATATGAAGCATCAAAACAAGCGATGAAAGAAGCAATTAGTGGACTTCAA
CCTTATCCAGACCATGCATTAATAGATGCTGTGCCGTTAGAGGGGTTAAACTGTACATATGAAGCTATTATTAAAGGGGA
TGCTGAAAGTGTGCACATAGCTGCAGCCAGTGTCTTAGCAAAAGTTGCACGTGATGATTGGATGAAAGAATTACATGAGA
AATATCCTTTATATGGATTTAATTCAAATATGGGCTATGGAACAAAGGAACACCTTCAAGCTATAGAAACCTATGGTGTA
ACTCCTTATCATCGTTATTCATTTGCCCCATTACATAAATACCGACATAACACGTTATTAAATTAA

Upstream 100 bases:

>100_bases
CTTCATGGATGTTTGCTGTGTTTACGATACCTTCATTTGAGAAATATGACTATTTATTTTTATTTTTTTAATCCGATAAA
TAAAGAAAGAGTGAAAAGAT

Downstream 100 bases:

>100_bases
TAATTGGAGGTGATTCTTTGAGCATTCATCGTTTATTAAGCGGTAAAGCTTTACAGAATGTTCATTTATCATCGCATCCA
CAGTTGAAGACTGGAGATGT

Product: ribonuclease H

Products: NA

Alternate protein names: RNase HII

Number of amino acids: Translated: 261; Mature: 261

Protein sequence:

>261_residues
MKPASIASIKSQLTNGELTQKQIEELYKDSRKGVQAILKRHEKQQEKQALLKQAFIKMNTYEKEAYRNGNHYIAGVDEVG
RGPLAGPVVAAAVILPKDFQLLGINDSKKLSEVQRNEFSHYIKQHAIAYQISFIDNTIIDKINIYEASKQAMKEAISGLQ
PYPDHALIDAVPLEGLNCTYEAIIKGDAESVHIAAASVLAKVARDDWMKELHEKYPLYGFNSNMGYGTKEHLQAIETYGV
TPYHRYSFAPLHKYRHNTLLN

Sequences:

>Translated_261_residues
MKPASIASIKSQLTNGELTQKQIEELYKDSRKGVQAILKRHEKQQEKQALLKQAFIKMNTYEKEAYRNGNHYIAGVDEVG
RGPLAGPVVAAAVILPKDFQLLGINDSKKLSEVQRNEFSHYIKQHAIAYQISFIDNTIIDKINIYEASKQAMKEAISGLQ
PYPDHALIDAVPLEGLNCTYEAIIKGDAESVHIAAASVLAKVARDDWMKELHEKYPLYGFNSNMGYGTKEHLQAIETYGV
TPYHRYSFAPLHKYRHNTLLN
>Mature_261_residues
MKPASIASIKSQLTNGELTQKQIEELYKDSRKGVQAILKRHEKQQEKQALLKQAFIKMNTYEKEAYRNGNHYIAGVDEVG
RGPLAGPVVAAAVILPKDFQLLGINDSKKLSEVQRNEFSHYIKQHAIAYQISFIDNTIIDKINIYEASKQAMKEAISGLQ
PYPDHALIDAVPLEGLNCTYEAIIKGDAESVHIAAASVLAKVARDDWMKELHEKYPLYGFNSNMGYGTKEHLQAIETYGV
TPYHRYSFAPLHKYRHNTLLN

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0164

COG function: function code L; Ribonuclease HII

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase HII family

Homologues:

Organism=Escherichia coli, GI1786380, Length=184, Percent_Identity=42.9347826086957, Blast_Score=145, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17536263, Length=223, Percent_Identity=30.0448430493274, Blast_Score=65, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH2_OCEIH (Q8EQZ4)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_692461.1
- ProteinModelPortal:   Q8EQZ4
- SMR:   Q8EQZ4
- GeneID:   1017821
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB1540
- NMPDR:   fig|221109.1.peg.1542
- HOGENOM:   HBG584843
- OMA:   RLGPTPI
- BioCyc:   OIHE221109:OB1540-MONOMER
- BRENDA:   3.1.26.4
- GO:   GO:0005737
- HAMAP:   MF_00052_B
- InterPro:   IPR022898
- InterPro:   IPR001352
- InterPro:   IPR012337
- PANTHER:   PTHR10954

Pfam domain/function: PF01351 RNase_HII; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 29460; Mature: 29460

Theoretical pI: Translated: 8.73; Mature: 8.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPASIASIKSQLTNGELTQKQIEELYKDSRKGVQAILKRHEKQQEKQALLKQAFIKMNT
CCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YEKEAYRNGNHYIAGVDEVGRGPLAGPVVAAAVILPKDFQLLGINDSKKLSEVQRNEFSH
HHHHHHHCCCCEEEEHHHHCCCCCCHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHH
YIKQHAIAYQISFIDNTIIDKINIYEASKQAMKEAISGLQPYPDHALIDAVPLEGLNCTY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCCCHH
EAIIKGDAESVHIAAASVLAKVARDDWMKELHEKYPLYGFNSNMGYGTKEHLQAIETYGV
HHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCHHHHHHHHHHCC
TPYHRYSFAPLHKYRHNTLLN
CCCCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure
MKPASIASIKSQLTNGELTQKQIEELYKDSRKGVQAILKRHEKQQEKQALLKQAFIKMNT
CCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YEKEAYRNGNHYIAGVDEVGRGPLAGPVVAAAVILPKDFQLLGINDSKKLSEVQRNEFSH
HHHHHHHCCCCEEEEHHHHCCCCCCHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHH
YIKQHAIAYQISFIDNTIIDKINIYEASKQAMKEAISGLQPYPDHALIDAVPLEGLNCTY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCCCHH
EAIIKGDAESVHIAAASVLAKVARDDWMKELHEKYPLYGFNSNMGYGTKEHLQAIETYGV
HHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCHHHHHHHHHHCC
TPYHRYSFAPLHKYRHNTLLN
CCCCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12235376