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The map label for this gene is trmD

Identifier: 23098991

GI number: 23098991

Start: 1579272

End: 1580009

Strand: Direct

Name: trmD

Synonym: OB1536

Alternate gene names: 23098991

Gene position: 1579272-1580009 (Clockwise)

Preceding gene: 23098990

Following gene: 23098992

Centisome position: 43.5

GC content: 37.13

Gene sequence:

>738_bases
ATGCATATCGATATATTGACATTGTTTCCAGAAATGTTTCACGGAGTATTTCAGTCCTCAATTTTGAATAAAGCGGTAGA
ACGACAACAATTTACTTACGACCTTGTAAATTTTAGAGAGTATAGTAAGAATAAACATCAGAAAGTCGATGATTATCCTT
ATGGTGGCGGAGCTGGAATGGTACTGACTCCTCAGCCAATATTTGATGCGGTTGAGGCTGTAAAAAGTAAGCGTAATTCA
AAACCTAGAGTTATTTTAATGTGTCCTCAGGGGGAAACATTAACCCAACAAAAATCTGAAGAACTTGCAAAAGAAGATCA
TTTGATTTTTATTTGTGGCCATTACGAAGGTTATGATGAACGAATTAGAGAGCACATTGTTACGGATGAAATATCCATTG
GTGATTATGTTCTAACTGGAGGAGAGTTAGGGTCGATGGTAGTAGTAGACAGTGTAGTTCGTTTATTACCAGATGTTCTA
GGAAATCAACAATCTGCCCCACAGGATTCGTTTTCTACAGGTCTTTTAGAACATCCGCACTATACAAGGCCAGCTGATTT
CAGAGGATATAAAGTACCGGAAGTATTACTTTCCGGAAATCATGCTAATATAGACTCCTGGAGAAGAAAACAATCGCTAA
TTCGAACCTGGAATCGCCGTAGGGATTTATTAGATAAAATTGAATTGACAGATGAAGAAAAAATAACGTTAAACACTGAT
ACGCCAGATGAAGTATAG

Upstream 100 bases:

>100_bases
CGAAAGATTTGTTAATCCCATATATTGAGCAGGTTGTGAAACAAGTAGATATTGAAAACAAAATAATCAAGGTTCAATTA
ATGGAAGGGATGCTTGATTA

Downstream 100 bases:

>100_bases
TAACAGCTCAGTCTAGGTCATAATTGCCAAGGTCTATTATAATAATTAATTAACGTAGGATTTTAAACCTTGGTGCTGAT
AGAAATGGAAATCGGCAACT

Product: tRNA methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase

Number of amino acids: Translated: 245; Mature: 245

Protein sequence:

>245_residues
MHIDILTLFPEMFHGVFQSSILNKAVERQQFTYDLVNFREYSKNKHQKVDDYPYGGGAGMVLTPQPIFDAVEAVKSKRNS
KPRVILMCPQGETLTQQKSEELAKEDHLIFICGHYEGYDERIREHIVTDEISIGDYVLTGGELGSMVVVDSVVRLLPDVL
GNQQSAPQDSFSTGLLEHPHYTRPADFRGYKVPEVLLSGNHANIDSWRRKQSLIRTWNRRRDLLDKIELTDEEKITLNTD
TPDEV

Sequences:

>Translated_245_residues
MHIDILTLFPEMFHGVFQSSILNKAVERQQFTYDLVNFREYSKNKHQKVDDYPYGGGAGMVLTPQPIFDAVEAVKSKRNS
KPRVILMCPQGETLTQQKSEELAKEDHLIFICGHYEGYDERIREHIVTDEISIGDYVLTGGELGSMVVVDSVVRLLPDVL
GNQQSAPQDSFSTGLLEHPHYTRPADFRGYKVPEVLLSGNHANIDSWRRKQSLIRTWNRRRDLLDKIELTDEEKITLNTD
TPDEV
>Mature_245_residues
MHIDILTLFPEMFHGVFQSSILNKAVERQQFTYDLVNFREYSKNKHQKVDDYPYGGGAGMVLTPQPIFDAVEAVKSKRNS
KPRVILMCPQGETLTQQKSEELAKEDHLIFICGHYEGYDERIREHIVTDEISIGDYVLTGGELGSMVVVDSVVRLLPDVL
GNQQSAPQDSFSTGLLEHPHYTRPADFRGYKVPEVLLSGNHANIDSWRRKQSLIRTWNRRRDLLDKIELTDEEKITLNTD
TPDEV

Specific function: Specifically methylates guanosine-37 in various tRNAs

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family

Homologues:

Organism=Escherichia coli, GI1788959, Length=233, Percent_Identity=48.068669527897, Blast_Score=223, Evalue=1e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMD_OCEIH (Q8CXH3)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_692457.1
- ProteinModelPortal:   Q8CXH3
- SMR:   Q8CXH3
- GeneID:   1017816
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB1536
- NMPDR:   fig|221109.1.peg.1538
- HOGENOM:   HBG285805
- OMA:   VCGRFEG
- BioCyc:   OIHE221109:OB1536-MONOMER
- BRENDA:   2.1.1.31
- GO:   GO:0005737
- HAMAP:   MF_00605
- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148
- Gene3D:   G3DSA:1.10.1270.20
- PIRSF:   PIRSF000386
- TIGRFAMs:   TIGR00088

Pfam domain/function: PF01746 tRNA_m1G_MT

EC number: =2.1.1.31

Molecular weight: Translated: 28046; Mature: 28046

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: NA

Important sites: BINDING 113-113

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHIDILTLFPEMFHGVFQSSILNKAVERQQFTYDLVNFREYSKNKHQKVDDYPYGGGAGM
CEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCE
VLTPQPIFDAVEAVKSKRNSKPRVILMCPQGETLTQQKSEELAKEDHLIFICGHYEGYDE
EECCHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHCCCCEEEEEECCCCHHH
RIREHIVTDEISIGDYVLTGGELGSMVVVDSVVRLLPDVLGNQQSAPQDSFSTGLLEHPH
HHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCC
YTRPADFRGYKVPEVLLSGNHANIDSWRRKQSLIRTWNRRRDLLDKIELTDEEKITLNTD
CCCCCCCCCCCCCHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCC
TPDEV
CCCCC
>Mature Secondary Structure
MHIDILTLFPEMFHGVFQSSILNKAVERQQFTYDLVNFREYSKNKHQKVDDYPYGGGAGM
CEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCE
VLTPQPIFDAVEAVKSKRNSKPRVILMCPQGETLTQQKSEELAKEDHLIFICGHYEGYDE
EECCHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHCCCCEEEEEECCCCHHH
RIREHIVTDEISIGDYVLTGGELGSMVVVDSVVRLLPDVLGNQQSAPQDSFSTGLLEHPH
HHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCC
YTRPADFRGYKVPEVLLSGNHANIDSWRRKQSLIRTWNRRRDLLDKIELTDEEKITLNTD
CCCCCCCCCCCCCHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCC
TPDEV
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12235376