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The map label for this gene is guaC

Identifier: 23098765

GI number: 23098765

Start: 1355657

End: 1356640

Strand: Reverse

Name: guaC

Synonym: OB1310

Alternate gene names: 23098765

Gene position: 1356640-1355657 (Counterclockwise)

Preceding gene: 23098793

Following gene: 23098763

Centisome position: 37.37

GC content: 34.86

Gene sequence:

>984_bases
ATGGAAAATGTATTCGATTATGAAGATATTCAGCTAATACCCGCAAAATGTGTGGTAAACAGTAGATCAGAATGTGATAC
GTCAGTTACTCTTGGAAATAGAACTTTTAAGTTACCTGTAGTACCTGCTAATATGCAAACAATTATTGATGAAAAGATTG
CTAAATATTTAGCAGAGAAAAACTACTTTTATATAATGCATCGTTTTGAACCTGAAAAAAGAATCGATTTTATTCAAGAC
ATGCAAGAATATAATTTATTCACCTCTATTAGTGTAGGTGTTAAAGAAGAAGAATACACATTTATTGAAGATTTAGCAGC
TAAACAATTGATTCCAGATTATATTACAATCGATATCGCCCATGGCCATTCAGATGCTGTAATAAAAATGATCAAACATA
TCAAAAACAACCTACCTAGTTCTTTTGTTATCGCTGGAAATGTAGGGACACCAGAAGCTGTTCGTGAATTAGAAAATGCA
GGTGCCGATGCTACAAAAGTTGGGATTGGTCCTGGTAAAGTTTGTATTACAAAAATCAAAACTGGTTTTGGAACTGGTGG
ATGGCAGTTAGCTGCATTACGTTGGTGTGCAAAAGCTGCTAGTAAACCTATTATTGCAGATGGTGGGATTCGTACTCATG
GAGACATTGCAAAATCAATTCGTTTTGGAGCATCCATGGTAATGATAGGCTCACTGTTTGCTGGTCATGAGGAATCGCCT
GGAGAAACAATCGAACAAGATGGTAAAAAAATTAAAGAATACTTTGGTTCGGCATCCGAATTTCAAAAAGGTGAAAAGAA
GAATGTAGAAGGTAAGAAAATGTATGTAGAGCATAAAGGCCCTCTTCAAGATACATTAACTGAAATGGAACAAGACCTTC
AATCTTCCATCTCTTATGCTGGTGGAAAACAACTTGATGCGATACGCAATGTAGATTACGTAGTTGTTAAGAATTCAATC
TTTAATGGAGATAAAGTATTTTAA

Upstream 100 bases:

>100_bases
TCAAGAATTATTGACATTTCTATATAAATACGTTAAATTACAAAAGGCGAACGTTTTTTAAATGAACTATTAAAATTATC
CGTATTTAGGAGTGGATATA

Downstream 100 bases:

>100_bases
TGATTACAGAACAGAACAAATAAAAATCGCAGTCATCAACCAATGATGACTGCGATTTTTATTATTCATGCACTTAAAAC
CTTCCTGAACGAAAGATAGA

Product: guanosine 5'-monophosphate oxidoreductase

Products: NA

Alternate protein names: Guanosine 5'-monophosphate oxidoreductase; Guanosine monophosphate reductase

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MENVFDYEDIQLIPAKCVVNSRSECDTSVTLGNRTFKLPVVPANMQTIIDEKIAKYLAEKNYFYIMHRFEPEKRIDFIQD
MQEYNLFTSISVGVKEEEYTFIEDLAAKQLIPDYITIDIAHGHSDAVIKMIKHIKNNLPSSFVIAGNVGTPEAVRELENA
GADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAASKPIIADGGIRTHGDIAKSIRFGASMVMIGSLFAGHEESP
GETIEQDGKKIKEYFGSASEFQKGEKKNVEGKKMYVEHKGPLQDTLTEMEQDLQSSISYAGGKQLDAIRNVDYVVVKNSI
FNGDKVF

Sequences:

>Translated_327_residues
MENVFDYEDIQLIPAKCVVNSRSECDTSVTLGNRTFKLPVVPANMQTIIDEKIAKYLAEKNYFYIMHRFEPEKRIDFIQD
MQEYNLFTSISVGVKEEEYTFIEDLAAKQLIPDYITIDIAHGHSDAVIKMIKHIKNNLPSSFVIAGNVGTPEAVRELENA
GADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAASKPIIADGGIRTHGDIAKSIRFGASMVMIGSLFAGHEESP
GETIEQDGKKIKEYFGSASEFQKGEKKNVEGKKMYVEHKGPLQDTLTEMEQDLQSSISYAGGKQLDAIRNVDYVVVKNSI
FNGDKVF
>Mature_327_residues
MENVFDYEDIQLIPAKCVVNSRSECDTSVTLGNRTFKLPVVPANMQTIIDEKIAKYLAEKNYFYIMHRFEPEKRIDFIQD
MQEYNLFTSISVGVKEEEYTFIEDLAAKQLIPDYITIDIAHGHSDAVIKMIKHIKNNLPSSFVIAGNVGTPEAVRELENA
GADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAASKPIIADGGIRTHGDIAKSIRFGASMVMIGSLFAGHEESP
GETIEQDGKKIKEYFGSASEFQKGEKKNVEGKKMYVEHKGPLQDTLTEMEQDLQSSISYAGGKQLDAIRNVDYVVVKNSI
FNGDKVF

Specific function: Catalyzes the irreversible NADPH-dependent deamination of GMP to IMP. It functions in the conversion of nucleobase, nucleoside and nucleotide derivatives of G to A nucleotides, and in maintaining the intracellular balance of A and G nucleotides

COG id: COG0516

COG function: function code F; IMP dehydrogenase/GMP reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IMPDH/GMPR family. GuaC type 2 subfamily

Homologues:

Organism=Homo sapiens, GI50541956, Length=340, Percent_Identity=34.1176470588235, Blast_Score=176, Evalue=3e-44,
Organism=Homo sapiens, GI50541954, Length=340, Percent_Identity=34.1176470588235, Blast_Score=176, Evalue=3e-44,
Organism=Homo sapiens, GI50541952, Length=340, Percent_Identity=34.1176470588235, Blast_Score=176, Evalue=3e-44,
Organism=Homo sapiens, GI50541948, Length=340, Percent_Identity=34.1176470588235, Blast_Score=176, Evalue=3e-44,
Organism=Homo sapiens, GI156104880, Length=338, Percent_Identity=30.7692307692308, Blast_Score=166, Evalue=2e-41,
Organism=Homo sapiens, GI66933016, Length=323, Percent_Identity=30.3405572755418, Blast_Score=118, Evalue=6e-27,
Organism=Homo sapiens, GI217035146, Length=269, Percent_Identity=32.3420074349442, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI217035150, Length=269, Percent_Identity=32.3420074349442, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI217035148, Length=269, Percent_Identity=32.3420074349442, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI156616279, Length=269, Percent_Identity=32.3420074349442, Blast_Score=113, Evalue=3e-25,
Organism=Homo sapiens, GI217035152, Length=269, Percent_Identity=32.3420074349442, Blast_Score=112, Evalue=3e-25,
Organism=Homo sapiens, GI34328928, Length=269, Percent_Identity=32.3420074349442, Blast_Score=112, Evalue=4e-25,
Organism=Homo sapiens, GI34328930, Length=269, Percent_Identity=31.9702602230483, Blast_Score=112, Evalue=4e-25,
Organism=Escherichia coli, GI1786293, Length=340, Percent_Identity=30.8823529411765, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI1788855, Length=215, Percent_Identity=39.0697674418605, Blast_Score=112, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI17560440, Length=321, Percent_Identity=32.0872274143302, Blast_Score=164, Evalue=4e-41,
Organism=Caenorhabditis elegans, GI71994385, Length=151, Percent_Identity=40.3973509933775, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI71994389, Length=151, Percent_Identity=40.3973509933775, Blast_Score=96, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6323464, Length=176, Percent_Identity=38.0681818181818, Blast_Score=107, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6322012, Length=246, Percent_Identity=31.7073170731707, Blast_Score=107, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6323585, Length=171, Percent_Identity=38.0116959064327, Blast_Score=106, Evalue=6e-24,
Organism=Drosophila melanogaster, GI24641071, Length=250, Percent_Identity=32.4, Blast_Score=118, Evalue=6e-27,
Organism=Drosophila melanogaster, GI24641073, Length=250, Percent_Identity=32.4, Blast_Score=118, Evalue=6e-27,
Organism=Drosophila melanogaster, GI28571163, Length=250, Percent_Identity=32.4, Blast_Score=118, Evalue=6e-27,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): GUAC_OCEIH (Q8ERJ2)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_692231.1
- ProteinModelPortal:   Q8ERJ2
- SMR:   Q8ERJ2
- GeneID:   1017488
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB1310
- NMPDR:   fig|221109.1.peg.1314
- HOGENOM:   HBG298985
- OMA:   PDYITID
- ProtClustDB:   PRK05458
- BioCyc:   OIHE221109:OB1310-MONOMER
- BRENDA:   1.7.1.7
- HAMAP:   MF_01511
- InterPro:   IPR013785
- InterPro:   IPR005994
- InterPro:   IPR015875
- InterPro:   IPR001093
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF036500
- TIGRFAMs:   TIGR01306

Pfam domain/function: PF00478 IMPDH

EC number: =1.7.1.7

Molecular weight: Translated: 36204; Mature: 36204

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: PS00487 IMP_DH_GMP_RED

Important sites: ACT_SITE 175-175

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENVFDYEDIQLIPAKCVVNSRSECDTSVTLGNRTFKLPVVPANMQTIIDEKIAKYLAEK
CCCCCCCCCCEEEEHHHHCCCCCCCCCEEEECCEEEEEEEECCHHHHHHHHHHHHHHHCC
NYFYIMHRFEPEKRIDFIQDMQEYNLFTSISVGVKEEEYTFIEDLAAKQLIPDYITIDIA
CEEEEEEECCCHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEEE
HGHSDAVIKMIKHIKNNLPSSFVIAGNVGTPEAVRELENAGADATKVGIGPGKVCITKIK
CCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHCCCCCEEECCCCCCEEEEEEC
TGFGTGGWQLAALRWCAKAASKPIIADGGIRTHGDIAKSIRFGASMVMIGSLFAGHEESP
CCCCCCCHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHCHHHHHHHHHHCCCCCCC
GETIEQDGKKIKEYFGSASEFQKGEKKNVEGKKMYVEHKGPLQDTLTEMEQDLQSSISYA
CHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHC
GGKQLDAIRNVDYVVVKNSIFNGDKVF
CCCHHHHHHCCCEEEEECCCCCCCCCC
>Mature Secondary Structure
MENVFDYEDIQLIPAKCVVNSRSECDTSVTLGNRTFKLPVVPANMQTIIDEKIAKYLAEK
CCCCCCCCCCEEEEHHHHCCCCCCCCCEEEECCEEEEEEEECCHHHHHHHHHHHHHHHCC
NYFYIMHRFEPEKRIDFIQDMQEYNLFTSISVGVKEEEYTFIEDLAAKQLIPDYITIDIA
CEEEEEEECCCHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEEE
HGHSDAVIKMIKHIKNNLPSSFVIAGNVGTPEAVRELENAGADATKVGIGPGKVCITKIK
CCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHCCCCCEEECCCCCCEEEEEEC
TGFGTGGWQLAALRWCAKAASKPIIADGGIRTHGDIAKSIRFGASMVMIGSLFAGHEESP
CCCCCCCHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHCHHHHHHHHHHCCCCCCC
GETIEQDGKKIKEYFGSASEFQKGEKKNVEGKKMYVEHKGPLQDTLTEMEQDLQSSISYA
CHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHC
GGKQLDAIRNVDYVVVKNSIFNGDKVF
CCCHHHHHHCCCEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12235376