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The map label for this gene is yueD [H]

Identifier: 23098567

GI number: 23098567

Start: 1165211

End: 1165972

Strand: Direct

Name: yueD [H]

Synonym: OB1112

Alternate gene names: 23098567

Gene position: 1165211-1165972 (Clockwise)

Preceding gene: 23098566

Following gene: 23098568

Centisome position: 32.09

GC content: 33.2

Gene sequence:

>762_bases
ATGAAAGCAGCAATAGTAACAGGAGTATCAAAAGGATTAGGAGAATCCATTGCAAAGCTGTATTTAGAATCGGGAGTAAT
GGTAATTGGAATATCACGCACTGATAACAAGAATTTATATTCAATAGCTGAAAACAACAATACAGAATTTCATCATGTAG
CTTGTGATTTATCGGATTTAAGTGAATATGATTCAGCACTTAACAAAGTGGATGAAATACTTTTCCAAGATCAAGTAGAA
TTAGGTACGATATATTTAGTGAATAATGCAGGTATCGTTGATCCAATAGACCAAGCAAAAAATATCACTAACGATACATT
ACAAAAGCATTTACAAGTGAATACTTTCGCACCAATGGCTTTTACAAATTATTATTTAAAAAAAGCAGAGGAGCGTCAAG
TTACATTAATTACTGCAATCGTCACATCCGGCGCAGCCAAGAAGCCTAAATTTGGATGGAGTGCTTACTGTTCTTCTAAA
GCAAGTATGAACATGTATACAGAAACGGTTGCTCTTGAACAAGATGAAGAGCAGACTGGGAACCAAATTATTGCTTTTAG
CCCTGGAGTAATGGATACAAACATGCAGGAAACAATCCGTTCTAGCTCAAAAGAGGCTTTTAAAGATGTAGAAGATTTCA
AGGGACTAAAAGATAATAATCTATTGAAGGATACAGATTTAATTGCAGGTGTTTTAATTGATATTATGACTGATGAAAAG
ATACAAAATGGAAAAATTTATGATGTCAAAGATTATATTTAA

Upstream 100 bases:

>100_bases
GCTCGTATACTATTGGAGTGAATTGTAATTACGATATATTTACGAATTGGCTATATTATAATATCGGGAAATAAAGCGTT
TATTTAAGGAGGGGTCAGGC

Downstream 100 bases:

>100_bases
GTGCGATTTCCAAAAGAAGGATGGAAAGGTACAAGAAGATCGAGGCTACATAGCTTGAGGTCCAGAACACATACCTTTAA
TTCATGAGGAAAGGAAATGC

Product: short chain dehydrogenase

Products: 2,3-Dihydroxybenzoate; H+; Nicotinamide adenine dinucleotide - reduced [C]

Alternate protein names: NA

Number of amino acids: Translated: 253; Mature: 253

Protein sequence:

>253_residues
MKAAIVTGVSKGLGESIAKLYLESGVMVIGISRTDNKNLYSIAENNNTEFHHVACDLSDLSEYDSALNKVDEILFQDQVE
LGTIYLVNNAGIVDPIDQAKNITNDTLQKHLQVNTFAPMAFTNYYLKKAEERQVTLITAIVTSGAAKKPKFGWSAYCSSK
ASMNMYTETVALEQDEEQTGNQIIAFSPGVMDTNMQETIRSSSKEAFKDVEDFKGLKDNNLLKDTDLIAGVLIDIMTDEK
IQNGKIYDVKDYI

Sequences:

>Translated_253_residues
MKAAIVTGVSKGLGESIAKLYLESGVMVIGISRTDNKNLYSIAENNNTEFHHVACDLSDLSEYDSALNKVDEILFQDQVE
LGTIYLVNNAGIVDPIDQAKNITNDTLQKHLQVNTFAPMAFTNYYLKKAEERQVTLITAIVTSGAAKKPKFGWSAYCSSK
ASMNMYTETVALEQDEEQTGNQIIAFSPGVMDTNMQETIRSSSKEAFKDVEDFKGLKDNNLLKDTDLIAGVLIDIMTDEK
IQNGKIYDVKDYI
>Mature_253_residues
MKAAIVTGVSKGLGESIAKLYLESGVMVIGISRTDNKNLYSIAENNNTEFHHVACDLSDLSEYDSALNKVDEILFQDQVE
LGTIYLVNNAGIVDPIDQAKNITNDTLQKHLQVNTFAPMAFTNYYLKKAEERQVTLITAIVTSGAAKKPKFGWSAYCSSK
ASMNMYTETVALEQDEEQTGNQIIAFSPGVMDTNMQETIRSSSKEAFKDVEDFKGLKDNNLLKDTDLIAGVLIDIMTDEK
IQNGKIYDVKDYI

Specific function: Reduces benzil stereospecifically to (S)-benzoin [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Caenorhabditis elegans, GI115534694, Length=197, Percent_Identity=27.9187817258883, Blast_Score=65, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6322226, Length=235, Percent_Identity=28.936170212766, Blast_Score=91, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6322227, Length=245, Percent_Identity=28.1632653061224, Blast_Score=80, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24641388, Length=232, Percent_Identity=25.8620689655172, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI116007236, Length=254, Percent_Identity=24.8031496062992, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24641232, Length=252, Percent_Identity=28.1746031746032, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24648693, Length=205, Percent_Identity=25.3658536585366, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI21355889, Length=205, Percent_Identity=25.3658536585366, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.3.1.28 [C]

Molecular weight: Translated: 28035; Mature: 28035

Theoretical pI: Translated: 4.43; Mature: 4.43

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAAIVTGVSKGLGESIAKLYLESGVMVIGISRTDNKNLYSIAENNNTEFHHVACDLSDL
CCCEEEHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCEEEEECCCCCEEEEEEECHHHH
SEYDSALNKVDEILFQDQVELGTIYLVNNAGIVDPIDQAKNITNDTLQKHLQVNTFAPMA
HHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCHHHHHCCCHHHHHHHHCCCCCCCHH
FTNYYLKKAEERQVTLITAIVTSGAAKKPKFGWSAYCSSKASMNMYTETVALEQDEEQTG
HHHHHHHCCCCCEEEEEEEEHHCCCCCCCCCCHHHHHCCCCCCHHHHHEEEECCCHHHCC
NQIIAFSPGVMDTNMQETIRSSSKEAFKDVEDFKGLKDNNLLKDTDLIAGVLIDIMTDEK
CEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCC
IQNGKIYDVKDYI
CCCCCEEECCCCC
>Mature Secondary Structure
MKAAIVTGVSKGLGESIAKLYLESGVMVIGISRTDNKNLYSIAENNNTEFHHVACDLSDL
CCCEEEHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCEEEEECCCCCEEEEEEECHHHH
SEYDSALNKVDEILFQDQVELGTIYLVNNAGIVDPIDQAKNITNDTLQKHLQVNTFAPMA
HHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCHHHHHCCCHHHHHHHHCCCCCCCHH
FTNYYLKKAEERQVTLITAIVTSGAAKKPKFGWSAYCSSKASMNMYTETVALEQDEEQTG
HHHHHHHCCCCCEEEEEEEEHHCCCCCCCCCCHHHHHCCCCCCHHHHHEEEECCCHHHCC
NQIIAFSPGVMDTNMQETIRSSSKEAFKDVEDFKGLKDNNLLKDTDLIAGVLIDIMTDEK
CEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCC
IQNGKIYDVKDYI
CCCCCEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 5550 [C]

Specific activity: 66

Km value (mM): 0.26 {methyl-2,3-dehydro-3-hydroxybenzoate}} 0.2 {3-oxocyclohexane-1-carboxylic} 0.3 {2,3-dihydro-2,3-dihydroxybenzoate}} [C]

Substrates: 2,3-Dihydro-2,3-dihydroxybenzoate; Nicotinamide adenine dinucleotide [C]

Specific reaction: 2,3-Dihydro-2,3-dihydroxybenzoate + Nicotinamide adenine dinucleotide <==> 2,3-Dihydroxybenzoate + H+ + Nicotinamide adenine dinucleotide - reduced [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]