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The map label for this gene is dapE [H]

Identifier: 23098566

GI number: 23098566

Start: 1163777

End: 1165138

Strand: Direct

Name: dapE [H]

Synonym: OB1111

Alternate gene names: 23098566

Gene position: 1163777-1165138 (Clockwise)

Preceding gene: 23098565

Following gene: 23098567

Centisome position: 32.06

GC content: 36.05

Gene sequence:

>1362_bases
ATGAGTGAAAAAGCATTACGTTATTTACAAGAACATCGAGAAGATATGTTAAACAGGTTATATCGCTTTTTGTCTATACC
AAGTGTAAGTACAGACTCCAAACATAAACAAGATATTGGCAAGGCAGCTGATTTTTTAATTACATACTTAGAAGAACTAT
CATTTACAAATATTGAAAAAGTAGAAACAGAAGGACATCCTCTCGTATACGGTGAATATATGGAAGCCGGGGAAGATGCA
CCTACTGTATTATTGTATGGTCATTATGATGTTCAGCCTGTTGATCCAATTGAATTATGGGATAGTGATCCATTTAAGCC
TGAACTTAGAGATGGACGTATTTTTGCCCGAGGTTCAAGTGATGATAAAGGTCAGGTGTTTATGCACTTGGCTGTATTTG
AAGCTTATTTAAAGACAGCAGGAAAGCTTCCGGTTAATGTCAAAGTTTGTATTGAAGGGGAAGAGGAGATCGGTAGTGAA
AATTTATATGATCTTCTACAAAAAAATAAAGAAAAATTCAAGGCAGATTTTGCAGTTATTTCTGATTCTGGAATGGCAGA
AAAAAATCAACCAACGATTTTATATGGTTTAAAAGGCTTTACTGGTATCGAAATTAAAGTAACTGGTCCAGATCATGATT
TACATTCTGGAATGTATGGTGGAGCAGTTAGAAATCCAATTATGGCTTTAAGTCATATCTTATCGTCTTTAAAAGATAAA
GACGAGGTAATCACTGTAGAAGGTTTCTATGACAATGTGGAAGACATTTCAGATGAAGAGCGTAAATTAATGGATCAGGC
ACCAGGTGAAGACTTTATTCATGCTACCGGTATCCCTGAAGTTGCATCAGAAAAAGGGTATACAGCGAAGGAACATACAA
TGGGTAGACCAACGCTAGAAGTAAACGGAATTTATGGAGGATATCAAGGAGAAGGTACAAAGACAATTATTCCATCAACT
GCCACAGCAAAAATCACTTGTAGATTAGTGCCAGGGCAAGATCCAGTAGATATTCAGGATAAATTAGTCAATCATGTAAA
TAATTCTACGCCTTCAGGTGTTACTGTAGAAGTGAAGAAAGAAAAACTCTCTGCTAAAGCGTATAAGGTGGAACCGACAC
ATCCATTGATACAAAAAGCAGCCAAAAGCTACACAAAAGCGTTTAATAAAGATACGGTTTTCTTACGCATGGGTGGTTCC
ATTCCTGTTGTAGAATGGTTTGAATCTATTTATCAATTCCCTATTGTATTATTAGGTTTTGGAACACCTGAAGATCGTTT
GCACTCACCAAACGAAAGTTTTCCATTAGATAGTTTTGATAAAGGTATGGAAACGCTCGTATACTATTGGAGTGAATTGT
AA

Upstream 100 bases:

>100_bases
TTTTTAGGCCAAGTGATGCTAGAAACAATTCATTGGTTGTACTATAATCATTGTAATACGAAGTTTGTTTTATTATGCAA
ATAATTGAGGAGGTAATAGG

Downstream 100 bases:

>100_bases
TTACGATATATTTACGAATTGGCTATATTATAATATCGGGAAATAAAGCGTTTATTTAAGGAGGGGTCAGGCATGAAAGC
AGCAATAGTAACAGGAGTAT

Product: hypothetical protein

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase [H]

Number of amino acids: Translated: 453; Mature: 452

Protein sequence:

>453_residues
MSEKALRYLQEHREDMLNRLYRFLSIPSVSTDSKHKQDIGKAADFLITYLEELSFTNIEKVETEGHPLVYGEYMEAGEDA
PTVLLYGHYDVQPVDPIELWDSDPFKPELRDGRIFARGSSDDKGQVFMHLAVFEAYLKTAGKLPVNVKVCIEGEEEIGSE
NLYDLLQKNKEKFKADFAVISDSGMAEKNQPTILYGLKGFTGIEIKVTGPDHDLHSGMYGGAVRNPIMALSHILSSLKDK
DEVITVEGFYDNVEDISDEERKLMDQAPGEDFIHATGIPEVASEKGYTAKEHTMGRPTLEVNGIYGGYQGEGTKTIIPST
ATAKITCRLVPGQDPVDIQDKLVNHVNNSTPSGVTVEVKKEKLSAKAYKVEPTHPLIQKAAKSYTKAFNKDTVFLRMGGS
IPVVEWFESIYQFPIVLLGFGTPEDRLHSPNESFPLDSFDKGMETLVYYWSEL

Sequences:

>Translated_453_residues
MSEKALRYLQEHREDMLNRLYRFLSIPSVSTDSKHKQDIGKAADFLITYLEELSFTNIEKVETEGHPLVYGEYMEAGEDA
PTVLLYGHYDVQPVDPIELWDSDPFKPELRDGRIFARGSSDDKGQVFMHLAVFEAYLKTAGKLPVNVKVCIEGEEEIGSE
NLYDLLQKNKEKFKADFAVISDSGMAEKNQPTILYGLKGFTGIEIKVTGPDHDLHSGMYGGAVRNPIMALSHILSSLKDK
DEVITVEGFYDNVEDISDEERKLMDQAPGEDFIHATGIPEVASEKGYTAKEHTMGRPTLEVNGIYGGYQGEGTKTIIPST
ATAKITCRLVPGQDPVDIQDKLVNHVNNSTPSGVTVEVKKEKLSAKAYKVEPTHPLIQKAAKSYTKAFNKDTVFLRMGGS
IPVVEWFESIYQFPIVLLGFGTPEDRLHSPNESFPLDSFDKGMETLVYYWSEL
>Mature_452_residues
SEKALRYLQEHREDMLNRLYRFLSIPSVSTDSKHKQDIGKAADFLITYLEELSFTNIEKVETEGHPLVYGEYMEAGEDAP
TVLLYGHYDVQPVDPIELWDSDPFKPELRDGRIFARGSSDDKGQVFMHLAVFEAYLKTAGKLPVNVKVCIEGEEEIGSEN
LYDLLQKNKEKFKADFAVISDSGMAEKNQPTILYGLKGFTGIEIKVTGPDHDLHSGMYGGAVRNPIMALSHILSSLKDKD
EVITVEGFYDNVEDISDEERKLMDQAPGEDFIHATGIPEVASEKGYTAKEHTMGRPTLEVNGIYGGYQGEGTKTIIPSTA
TAKITCRLVPGQDPVDIQDKLVNHVNNSTPSGVTVEVKKEKLSAKAYKVEPTHPLIQKAAKSYTKAFNKDTVFLRMGGSI
PVVEWFESIYQFPIVLLGFGTPEDRLHSPNESFPLDSFDKGMETLVYYWSEL

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily [H]

Homologues:

Organism=Homo sapiens, GI271398239, Length=467, Percent_Identity=30.6209850107066, Blast_Score=211, Evalue=1e-54,
Organism=Homo sapiens, GI21071039, Length=480, Percent_Identity=27.5, Blast_Score=184, Evalue=2e-46,
Organism=Homo sapiens, GI271398277, Length=321, Percent_Identity=30.5295950155763, Blast_Score=146, Evalue=4e-35,
Organism=Caenorhabditis elegans, GI17563146, Length=455, Percent_Identity=32.0879120879121, Blast_Score=222, Evalue=3e-58,
Organism=Caenorhabditis elegans, GI193211059, Length=477, Percent_Identity=29.3501048218029, Blast_Score=192, Evalue=3e-49,
Organism=Caenorhabditis elegans, GI17538642, Length=442, Percent_Identity=24.8868778280543, Blast_Score=73, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6321159, Length=456, Percent_Identity=29.1666666666667, Blast_Score=196, Evalue=7e-51,
Organism=Saccharomyces cerevisiae, GI6319758, Length=456, Percent_Identity=26.7543859649123, Blast_Score=155, Evalue=1e-38,
Organism=Drosophila melanogaster, GI24585880, Length=468, Percent_Identity=32.9059829059829, Blast_Score=220, Evalue=2e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001261
- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.18 [H]

Molecular weight: Translated: 50652; Mature: 50521

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEKALRYLQEHREDMLNRLYRFLSIPSVSTDSKHKQDIGKAADFLITYLEELSFTNIEK
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHH
VETEGHPLVYGEYMEAGEDAPTVLLYGHYDVQPVDPIELWDSDPFKPELRDGRIFARGSS
HCCCCCCEEEEHHHHCCCCCCEEEEEECCCCCCCCHHHCCCCCCCCCCCCCCEEEEECCC
DDKGQVFMHLAVFEAYLKTAGKLPVNVKVCIEGEEEIGSENLYDLLQKNKEKFKADFAVI
CCCCCEEHHHHHHHHHHHHCCCCCEEEEEEECCHHHCCCHHHHHHHHHHHHHHHHCEEEE
SDSGMAEKNQPTILYGLKGFTGIEIKVTGPDHDLHSGMYGGAVRNPIMALSHILSSLKDK
ECCCCCCCCCCEEEEECCCCEEEEEEEECCCCHHHCCCCCCHHHHHHHHHHHHHHHCCCC
DEVITVEGFYDNVEDISDEERKLMDQAPGEDFIHATGIPEVASEKGYTAKEHTMGRPTLE
CCEEEEECCCCCHHHCCHHHHHHHHHCCCCCCEEECCCCHHHHCCCCCCHHHCCCCCCEE
VNGIYGGYQGEGTKTIIPSTATAKITCRLVPGQDPVDIQDKLVNHVNNSTPSGVTVEVKK
EECEECCCCCCCCEEECCCCCEEEEEEEEECCCCCCCHHHHHHHHHCCCCCCCEEEEEEH
EKLSAKAYKVEPTHPLIQKAAKSYTKAFNKDTVFLRMGGSIPVVEWFESIYQFPIVLLGF
HHHCCCEEEECCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHCCEEEEEC
GTPEDRLHSPNESFPLDSFDKGMETLVYYWSEL
CCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SEKALRYLQEHREDMLNRLYRFLSIPSVSTDSKHKQDIGKAADFLITYLEELSFTNIEK
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHH
VETEGHPLVYGEYMEAGEDAPTVLLYGHYDVQPVDPIELWDSDPFKPELRDGRIFARGSS
HCCCCCCEEEEHHHHCCCCCCEEEEEECCCCCCCCHHHCCCCCCCCCCCCCCEEEEECCC
DDKGQVFMHLAVFEAYLKTAGKLPVNVKVCIEGEEEIGSENLYDLLQKNKEKFKADFAVI
CCCCCEEHHHHHHHHHHHHCCCCCEEEEEEECCHHHCCCHHHHHHHHHHHHHHHHCEEEE
SDSGMAEKNQPTILYGLKGFTGIEIKVTGPDHDLHSGMYGGAVRNPIMALSHILSSLKDK
ECCCCCCCCCCEEEEECCCCEEEEEEEECCCCHHHCCCCCCHHHHHHHHHHHHHHHCCCC
DEVITVEGFYDNVEDISDEERKLMDQAPGEDFIHATGIPEVASEKGYTAKEHTMGRPTLE
CCEEEEECCCCCHHHCCHHHHHHHHHCCCCCCEEECCCCHHHHCCCCCCHHHCCCCCCEE
VNGIYGGYQGEGTKTIIPSTATAKITCRLVPGQDPVDIQDKLVNHVNNSTPSGVTVEVKK
EECEECCCCCCCCEEECCCCCEEEEEEEEECCCCCCCHHHHHHHHHCCCCCCCEEEEEEH
EKLSAKAYKVEPTHPLIQKAAKSYTKAFNKDTVFLRMGGSIPVVEWFESIYQFPIVLLGF
HHHCCCEEEECCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHCCEEEEEC
GTPEDRLHSPNESFPLDSFDKGMETLVYYWSEL
CCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA