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The map label for this gene is odhB [H]

Identifier: 23098545

GI number: 23098545

Start: 1139507

End: 1140775

Strand: Direct

Name: odhB [H]

Synonym: OB1090

Alternate gene names: 23098545

Gene position: 1139507-1140775 (Clockwise)

Preceding gene: 23098544

Following gene: 23098546

Centisome position: 31.39

GC content: 38.22

Gene sequence:

>1269_bases
GTGCAGGAAATTAAAATCCCAGAATTAGCAGAATCCATTACAGAAGGTACAATAGCAGAATGGCTTGTCAAAAAAGGTGA
CAAAGTTGAAAAAGGTGATCCAGTAGTAGAATTAGAGACGGATAAAGTAAATGTAGAAGTAAATGCAGAATTTTCCGGAG
TCATCACTGAAATTATTAGTGAAGAGGGTGATGATGTAACTGTTGGAGATACGATAGCTAAGTTAGATGAAAATGGTGAA
GCTGGTTCCAATTCAGATGAATCAGAACCTAAAGAAGAACCTAAACAAGAAGAAAAGCAAGAAGACGATAAGAAGAAAGC
GTCTGAAACAGAAACTTCCAAGGGAGCGGAAACCAAGTCTGAAAGTAATGGAGAAGTGATCGCATCTCCTGCAGCTAGAA
AACGAGCACGTGAATTAAATATTGATTTAAGCAGTGTTCAATCACGTGACCCATTAGGACGTGTACGCACAGAAGATGTT
GAAGCACAAGCACAGGCGAATAAACAACCTGCCGAGAAGAAACAAGAGAAAAAAGATGCTCCAAAATCGGAGAAAACGGA
GTTTGACAAACCGGTAGAAAGAGTGAAAATGACTCGTCGCCGTCAAACAATCGCAAAAAATCTTGTAGAAGTACAGCATA
ATACGGCAATGCTAACTACTTTTAACGAAGTAGATATGACAGCGGTTATGGAATTACGAAAACAACGTAAAGATAAATTC
TTAGACAAAAATGGCGTTAAGTTAGGATTCATGTCCTTCTTTACAAAAGCGGTTGTAGGGGCTCTAAAAGAATTCCCATT
ATTAAATGCTGAAATTCAGGGAAATGAATTAGTCATTAAGAAATTCTATGACATCGGTATTGCTGTCTCCACGGATGATG
GACTTGTAGTGCCAGTAGTCAGAGATGCAGACCGTAAAGATTTTGCTGGGATTGAGCAAGATATTAACGATTTAGGCATA
AAAGCACGAGATAATAAATTGACGTTAAAAGACTTGCAAGGTGGATCATTTACGATTACAAACGGAGGGACATTTGGTTC
CATGATGTCTACACCAATCTTAAATGCACCTCAAGTAGGTATCTTAGGAATGCACAATATAGTAAAACGTGCAATGGTAA
TGCCAGATGATTCTATTGAAGTTCGTCCTATGATGTACTTGGCTCTATCTTATGACCACCGTATTGTCGACGGTAAGGAA
GCTGTACAATTCCTTGTTCGAATTAAGCAAATGTTAGAAGATCCATATGATCTTTTACTTGAAGGTTAA

Upstream 100 bases:

>100_bases
GATCGTTCAGCACCAGCTGGAGGTATTCCGACTGTACACAAAACGGCTCAAAATAAAATTATCAAACAAGCATTGAATCA
ATCTGAAGGAGGAAAATCCA

Downstream 100 bases:

>100_bases
TATAAATAATAGTTATTTAGAAAAACAGGGTTATAGATATGACCCTGTTTTTCTGTGTTAGATAAATAAAAATGGAATTT
ATTACCTGATGCAAGCGCCT

Product: dihydrolipoamide succinyltransferase

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 422; Mature: 422

Protein sequence:

>422_residues
MQEIKIPELAESITEGTIAEWLVKKGDKVEKGDPVVELETDKVNVEVNAEFSGVITEIISEEGDDVTVGDTIAKLDENGE
AGSNSDESEPKEEPKQEEKQEDDKKKASETETSKGAETKSESNGEVIASPAARKRARELNIDLSSVQSRDPLGRVRTEDV
EAQAQANKQPAEKKQEKKDAPKSEKTEFDKPVERVKMTRRRQTIAKNLVEVQHNTAMLTTFNEVDMTAVMELRKQRKDKF
LDKNGVKLGFMSFFTKAVVGALKEFPLLNAEIQGNELVIKKFYDIGIAVSTDDGLVVPVVRDADRKDFAGIEQDINDLGI
KARDNKLTLKDLQGGSFTITNGGTFGSMMSTPILNAPQVGILGMHNIVKRAMVMPDDSIEVRPMMYLALSYDHRIVDGKE
AVQFLVRIKQMLEDPYDLLLEG

Sequences:

>Translated_422_residues
MQEIKIPELAESITEGTIAEWLVKKGDKVEKGDPVVELETDKVNVEVNAEFSGVITEIISEEGDDVTVGDTIAKLDENGE
AGSNSDESEPKEEPKQEEKQEDDKKKASETETSKGAETKSESNGEVIASPAARKRARELNIDLSSVQSRDPLGRVRTEDV
EAQAQANKQPAEKKQEKKDAPKSEKTEFDKPVERVKMTRRRQTIAKNLVEVQHNTAMLTTFNEVDMTAVMELRKQRKDKF
LDKNGVKLGFMSFFTKAVVGALKEFPLLNAEIQGNELVIKKFYDIGIAVSTDDGLVVPVVRDADRKDFAGIEQDINDLGI
KARDNKLTLKDLQGGSFTITNGGTFGSMMSTPILNAPQVGILGMHNIVKRAMVMPDDSIEVRPMMYLALSYDHRIVDGKE
AVQFLVRIKQMLEDPYDLLLEG
>Mature_422_residues
MQEIKIPELAESITEGTIAEWLVKKGDKVEKGDPVVELETDKVNVEVNAEFSGVITEIISEEGDDVTVGDTIAKLDENGE
AGSNSDESEPKEEPKQEEKQEDDKKKASETETSKGAETKSESNGEVIASPAARKRARELNIDLSSVQSRDPLGRVRTEDV
EAQAQANKQPAEKKQEKKDAPKSEKTEFDKPVERVKMTRRRQTIAKNLVEVQHNTAMLTTFNEVDMTAVMELRKQRKDKF
LDKNGVKLGFMSFFTKAVVGALKEFPLLNAEIQGNELVIKKFYDIGIAVSTDDGLVVPVVRDADRKDFAGIEQDINDLGI
KARDNKLTLKDLQGGSFTITNGGTFGSMMSTPILNAPQVGILGMHNIVKRAMVMPDDSIEVRPMMYLALSYDHRIVDGKE
AVQFLVRIKQMLEDPYDLLLEG

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components:2- oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2)

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=231, Percent_Identity=52.8138528138528, Blast_Score=249, Evalue=4e-66,
Organism=Homo sapiens, GI31711992, Length=437, Percent_Identity=28.604118993135, Blast_Score=159, Evalue=3e-39,
Organism=Homo sapiens, GI110671329, Length=436, Percent_Identity=27.5229357798165, Blast_Score=155, Evalue=7e-38,
Organism=Homo sapiens, GI203098753, Length=453, Percent_Identity=26.9315673289183, Blast_Score=143, Evalue=2e-34,
Organism=Homo sapiens, GI203098816, Length=453, Percent_Identity=26.9315673289183, Blast_Score=142, Evalue=4e-34,
Organism=Homo sapiens, GI260898739, Length=166, Percent_Identity=33.1325301204819, Blast_Score=89, Evalue=8e-18,
Organism=Escherichia coli, GI1786946, Length=419, Percent_Identity=44.3914081145585, Blast_Score=335, Evalue=3e-93,
Organism=Escherichia coli, GI1786305, Length=437, Percent_Identity=30.2059496567506, Blast_Score=182, Evalue=3e-47,
Organism=Caenorhabditis elegans, GI25146366, Length=417, Percent_Identity=41.2470023980815, Blast_Score=296, Evalue=1e-80,
Organism=Caenorhabditis elegans, GI17560088, Length=442, Percent_Identity=28.0542986425339, Blast_Score=160, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI17537937, Length=428, Percent_Identity=26.8691588785047, Blast_Score=159, Evalue=3e-39,
Organism=Caenorhabditis elegans, GI17538894, Length=228, Percent_Identity=30.2631578947368, Blast_Score=100, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6320352, Length=420, Percent_Identity=40.7142857142857, Blast_Score=300, Evalue=2e-82,
Organism=Saccharomyces cerevisiae, GI6324258, Length=452, Percent_Identity=27.8761061946903, Blast_Score=164, Evalue=3e-41,
Organism=Drosophila melanogaster, GI24645909, Length=223, Percent_Identity=51.1210762331839, Blast_Score=241, Evalue=7e-64,
Organism=Drosophila melanogaster, GI18859875, Length=436, Percent_Identity=28.6697247706422, Blast_Score=156, Evalue=2e-38,
Organism=Drosophila melanogaster, GI24582497, Length=232, Percent_Identity=32.7586206896552, Blast_Score=124, Evalue=9e-29,
Organism=Drosophila melanogaster, GI20129315, Length=232, Percent_Identity=32.7586206896552, Blast_Score=124, Evalue=9e-29,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 46873; Mature: 46873

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQEIKIPELAESITEGTIAEWLVKKGDKVEKGDPVVELETDKVNVEVNAEFSGVITEIIS
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCEEEEEECCCHHHHHHHHHH
EEGDDVTVGDTIAKLDENGEAGSNSDESEPKEEPKQEEKQEDDKKKASETETSKGAETKS
CCCCCEEHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCC
ESNGEVIASPAARKRARELNIDLSSVQSRDPLGRVRTEDVEAQAQANKQPAEKKQEKKDA
CCCCCEEECCHHHHHHHHCCCCHHHHCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHCC
PKSEKTEFDKPVERVKMTRRRQTIAKNLVEVQHNTAMLTTFNEVDMTAVMELRKQRKDKF
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHHHHH
LDKNGVKLGFMSFFTKAVVGALKEFPLLNAEIQGNELVIKKFYDIGIAVSTDDGLVVPVV
HCCCCCEEHHHHHHHHHHHHHHHHCCCCCCEECCCEEEEEEHHHCEEEEECCCCEEEEEE
RDADRKDFAGIEQDINDLGIKARDNKLTLKDLQGGSFTITNGGTFGSMMSTPILNAPQVG
CCCCCCHHHHHHHHHHHCCCEECCCEEEEEECCCCEEEEECCCCCHHHHHCCCCCCCCCC
ILGMHNIVKRAMVMPDDSIEVRPMMYLALSYDHRIVDGKEAVQFLVRIKQMLEDPYDLLL
HHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCEECCHHHHHHHHHHHHHHHCCCHHHHC
EG
CC
>Mature Secondary Structure
MQEIKIPELAESITEGTIAEWLVKKGDKVEKGDPVVELETDKVNVEVNAEFSGVITEIIS
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCEEEEEECCCHHHHHHHHHH
EEGDDVTVGDTIAKLDENGEAGSNSDESEPKEEPKQEEKQEDDKKKASETETSKGAETKS
CCCCCEEHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCC
ESNGEVIASPAARKRARELNIDLSSVQSRDPLGRVRTEDVEAQAQANKQPAEKKQEKKDA
CCCCCEEECCHHHHHHHHCCCCHHHHCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHCC
PKSEKTEFDKPVERVKMTRRRQTIAKNLVEVQHNTAMLTTFNEVDMTAVMELRKQRKDKF
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHHHHH
LDKNGVKLGFMSFFTKAVVGALKEFPLLNAEIQGNELVIKKFYDIGIAVSTDDGLVVPVV
HCCCCCEEHHHHHHHHHHHHHHHHCCCCCCEECCCEEEEEEHHHCEEEEECCCCEEEEEE
RDADRKDFAGIEQDINDLGIKARDNKLTLKDLQGGSFTITNGGTFGSMMSTPILNAPQVG
CCCCCCHHHHHHHHHHHCCCEECCCEEEEEECCCCEEEEECCCCCHHHHHCCCCCCCCCC
ILGMHNIVKRAMVMPDDSIEVRPMMYLALSYDHRIVDGKEAVQFLVRIKQMLEDPYDLLL
HHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCEECCHHHHHHHHHHHHHHHCCCHHHHC
EG
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2500417; 9384377 [H]