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The map label for this gene is glsA

Identifier: 23098325

GI number: 23098325

Start: 923668

End: 924663

Strand: Direct

Name: glsA

Synonym: OB0870

Alternate gene names: 23098325

Gene position: 923668-924663 (Clockwise)

Preceding gene: 23098324

Following gene: 23098326

Centisome position: 25.44

GC content: 37.75

Gene sequence:

>996_bases
ATGGTGCAGGGATCGGTTAATTTAAATACAGAGAGAGGGAAAGAGTGGTTACAAGATTATGTTGATAATTGGGTGAACTT
CTATCAGAAACAAACAGATGAAGGGAAAGTAGCTAGTTACATACCAAGGCTTGAACATGCTGATCCCAATGCTTTAGGTA
TTTCTATAATTGGTAAAAATGGTACGGTCATCCGTTCTGGAGATACAGATCTAGAATTTTCCATTCAAAGTATATCGAAA
GTGTTAAGTTTTATTGTAGCTTGCATGGAAAGAGGCCTCTCCTATGTCCTTCAAAGGGTTGATGTAGAACCTACTGGAGA
ATCTTTTAACTCAATTATGCATTTAGAGATCAATCAACCAAAAAAACCATTTAATCCACTTGTGAATTCCGGGGCTATTA
CAGTTTCTTCTTTATTAAATGGTAGAACCTCTGACCAAAAACTAGAACCCCTATATCAATTATTAGAAAAAATACTTGGA
CATCGACCTGAAATTGATGTGGAAGTATATGTTTCTGAGCGAGATACTTCGATGCGAAATAGAGCAATTGGATATTACCT
ACTGGAAGAAGGTTACCTAGAGTCTGATTTGAGTATTACGTTGGAAACCTATTTTAAACACTGTTCATTAAACGTAACAG
TGGATGACTTGGCTATGATAGGTTTAGTGCTATCTAATGATGGAGTACATCCGAATACAGATGAACCACTGATTCCAAAA
CAAATTGCTAGAGTCGCGAAATCACTCATGCTTACATGCGGCATGTATGATGCGTCAGGGAAGTTTGCTTCATATGTAGG
TATTCCTGCTAAAAGTGGAGTATCTGGAGGAATACTTGCAGTGGTCCCACCAAGAGTCCGCGATCAAAATCTTCCATTTC
TAGAAGGATGTGGTATTGGAGTATTTGGACCAGCTTTGGATAAGCAAGGGAATAGTATTGCAGGCATTAAACTCCTGCGA
CATATAGCAAATCAATGGGATTTAAGTTTATTCTAA

Upstream 100 bases:

>100_bases
TTCTGAGTGTTTTTTTGTATGGATTAGAAACAAATAAGGGTAAAATAAAAAAGATAAGGGAATAGGTAGATATAGCAATA
TAGAAATGAGGTGTTTAAAA

Downstream 100 bases:

>100_bases
GGGAGGAATAATCTTTGTGTGGCAGATATACGTTATTAGCAGATGAATTAGCAATTAAAGAAGCGTTTGGAATACAGCAA
TCGTTGGACTTGTATGAGCC

Product: glutaminase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MVQGSVNLNTERGKEWLQDYVDNWVNFYQKQTDEGKVASYIPRLEHADPNALGISIIGKNGTVIRSGDTDLEFSIQSISK
VLSFIVACMERGLSYVLQRVDVEPTGESFNSIMHLEINQPKKPFNPLVNSGAITVSSLLNGRTSDQKLEPLYQLLEKILG
HRPEIDVEVYVSERDTSMRNRAIGYYLLEEGYLESDLSITLETYFKHCSLNVTVDDLAMIGLVLSNDGVHPNTDEPLIPK
QIARVAKSLMLTCGMYDASGKFASYVGIPAKSGVSGGILAVVPPRVRDQNLPFLEGCGIGVFGPALDKQGNSIAGIKLLR
HIANQWDLSLF

Sequences:

>Translated_331_residues
MVQGSVNLNTERGKEWLQDYVDNWVNFYQKQTDEGKVASYIPRLEHADPNALGISIIGKNGTVIRSGDTDLEFSIQSISK
VLSFIVACMERGLSYVLQRVDVEPTGESFNSIMHLEINQPKKPFNPLVNSGAITVSSLLNGRTSDQKLEPLYQLLEKILG
HRPEIDVEVYVSERDTSMRNRAIGYYLLEEGYLESDLSITLETYFKHCSLNVTVDDLAMIGLVLSNDGVHPNTDEPLIPK
QIARVAKSLMLTCGMYDASGKFASYVGIPAKSGVSGGILAVVPPRVRDQNLPFLEGCGIGVFGPALDKQGNSIAGIKLLR
HIANQWDLSLF
>Mature_331_residues
MVQGSVNLNTERGKEWLQDYVDNWVNFYQKQTDEGKVASYIPRLEHADPNALGISIIGKNGTVIRSGDTDLEFSIQSISK
VLSFIVACMERGLSYVLQRVDVEPTGESFNSIMHLEINQPKKPFNPLVNSGAITVSSLLNGRTSDQKLEPLYQLLEKILG
HRPEIDVEVYVSERDTSMRNRAIGYYLLEEGYLESDLSITLETYFKHCSLNVTVDDLAMIGLVLSNDGVHPNTDEPLIPK
QIARVAKSLMLTCGMYDASGKFASYVGIPAKSGVSGGILAVVPPRVRDQNLPFLEGCGIGVFGPALDKQGNSIAGIKLLR
HIANQWDLSLF

Specific function: Unknown

COG id: COG2066

COG function: function code E; Glutaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutaminase family

Homologues:

Organism=Homo sapiens, GI156104878, Length=327, Percent_Identity=37.0030581039755, Blast_Score=193, Evalue=2e-49,
Organism=Homo sapiens, GI20336214, Length=303, Percent_Identity=35.973597359736, Blast_Score=177, Evalue=1e-44,
Organism=Escherichia coli, GI1787804, Length=302, Percent_Identity=39.0728476821192, Blast_Score=201, Evalue=8e-53,
Organism=Escherichia coli, GI1786693, Length=316, Percent_Identity=34.1772151898734, Blast_Score=181, Evalue=8e-47,
Organism=Caenorhabditis elegans, GI17532727, Length=322, Percent_Identity=34.472049689441, Blast_Score=186, Evalue=1e-47,
Organism=Caenorhabditis elegans, GI17507019, Length=303, Percent_Identity=34.6534653465347, Blast_Score=185, Evalue=3e-47,
Organism=Caenorhabditis elegans, GI193204073, Length=326, Percent_Identity=34.9693251533742, Blast_Score=183, Evalue=1e-46,
Organism=Caenorhabditis elegans, GI193204075, Length=328, Percent_Identity=34.4512195121951, Blast_Score=181, Evalue=6e-46,
Organism=Drosophila melanogaster, GI281363241, Length=305, Percent_Identity=32.7868852459016, Blast_Score=179, Evalue=3e-45,
Organism=Drosophila melanogaster, GI281363239, Length=305, Percent_Identity=32.7868852459016, Blast_Score=179, Evalue=3e-45,
Organism=Drosophila melanogaster, GI116008307, Length=305, Percent_Identity=32.7868852459016, Blast_Score=179, Evalue=3e-45,
Organism=Drosophila melanogaster, GI24653162, Length=305, Percent_Identity=32.7868852459016, Blast_Score=179, Evalue=3e-45,
Organism=Drosophila melanogaster, GI24653156, Length=305, Percent_Identity=32.7868852459016, Blast_Score=179, Evalue=3e-45,
Organism=Drosophila melanogaster, GI24653158, Length=305, Percent_Identity=32.7868852459016, Blast_Score=178, Evalue=4e-45,
Organism=Drosophila melanogaster, GI24653164, Length=305, Percent_Identity=32.7868852459016, Blast_Score=178, Evalue=4e-45,
Organism=Drosophila melanogaster, GI24653166, Length=305, Percent_Identity=32.7868852459016, Blast_Score=178, Evalue=4e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLSA_OCEIH (Q8CV87)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_691791.1
- ProteinModelPortal:   Q8CV87
- SMR:   Q8CV87
- GeneID:   1016494
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB0870
- NMPDR:   fig|221109.1.peg.876
- HOGENOM:   HBG512335
- OMA:   YLSERET
- ProtClustDB:   PRK12357
- BioCyc:   OIHE221109:OB0870-MONOMER
- BRENDA:   3.5.1.2
- HAMAP:   MF_00313
- InterPro:   IPR012338
- InterPro:   IPR015868
- Gene3D:   G3DSA:3.40.710.20
- PANTHER:   PTHR12544
- TIGRFAMs:   TIGR03814

Pfam domain/function: PF04960 Glutaminase; SSF56601 PBP_transp_fold

EC number: =3.5.1.2

Molecular weight: Translated: 36507; Mature: 36507

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVQGSVNLNTERGKEWLQDYVDNWVNFYQKQTDEGKVASYIPRLEHADPNALGISIIGKN
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCEEEEEEECCC
GTVIRSGDTDLEFSIQSISKVLSFIVACMERGLSYVLQRVDVEPTGESFNSIMHLEINQP
CCEEECCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCC
KKPFNPLVNSGAITVSSLLNGRTSDQKLEPLYQLLEKILGHRPEIDVEVYVSERDTSMRN
CCCCCHHHCCCCEEHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHH
RAIGYYLLEEGYLESDLSITLETYFKHCSLNVTVDDLAMIGLVLSNDGVHPNTDEPLIPK
HHHEEEEEECCCCCCCCEEEHHHHHHHCCCCCCHHHHHHHHHHEECCCCCCCCCCCCCHH
QIARVAKSLMLTCGMYDASGKFASYVGIPAKSGVSGGILAVVPPRVRDQNLPFLEGCGIG
HHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCEEEEECCCCCCCCCCEECCCCCC
VFGPALDKQGNSIAGIKLLRHIANQWDLSLF
CCCCCCCCCCCCHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MVQGSVNLNTERGKEWLQDYVDNWVNFYQKQTDEGKVASYIPRLEHADPNALGISIIGKN
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCEEEEEEECCC
GTVIRSGDTDLEFSIQSISKVLSFIVACMERGLSYVLQRVDVEPTGESFNSIMHLEINQP
CCEEECCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCC
KKPFNPLVNSGAITVSSLLNGRTSDQKLEPLYQLLEKILGHRPEIDVEVYVSERDTSMRN
CCCCCHHHCCCCEEHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHH
RAIGYYLLEEGYLESDLSITLETYFKHCSLNVTVDDLAMIGLVLSNDGVHPNTDEPLIPK
HHHEEEEEECCCCCCCCEEEHHHHHHHCCCCCCHHHHHHHHHHEECCCCCCCCCCCCCHH
QIARVAKSLMLTCGMYDASGKFASYVGIPAKSGVSGGILAVVPPRVRDQNLPFLEGCGIG
HHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCEEEEECCCCCCCCCCEECCCCCC
VFGPALDKQGNSIAGIKLLRHIANQWDLSLF
CCCCCCCCCCCCHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12235376