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The map label for this gene is ampD [H]

Identifier: 23098285

GI number: 23098285

Start: 881928

End: 882632

Strand: Direct

Name: ampD [H]

Synonym: OB0830

Alternate gene names: 23098285

Gene position: 881928-882632 (Clockwise)

Preceding gene: 23098284

Following gene: 23098286

Centisome position: 24.29

GC content: 34.75

Gene sequence:

>705_bases
ATGTTTAATTTATGGAAAATCAGTTTTGTAATTGTAGCATTATTTTTTTTAGTAGCATGTAAACAAGAAGTAACAAAGGA
TACATTAGAAGTAGCGGCTAAAGAGGAAAAGGTAGAATCGGTAGAAGTGAATTTTGAAGAAAAGATGATAGATATGTACC
TTCCAGAAGAAAATTCCGAACCAAGAGATTCATTAGTAACCCATGTAATGTTGCATTTTAGTAGTAATGTAATCGAGAAG
CCAGAGGATCCCTTTCAAGTGGAAGATATTTATAAGGTATTCCATGATTATGGGGTATCGACACACTATGTAATTGATCG
AACGGGTGAAATATATCGATTTGTGTCCGAAGATAGAAATGCTTACCATGCTGGTGAAGGAAAGATAGAAGGGGTTCCAA
AGTATGAAAATAATATGAACCGTTATTCTATTGGAATTGAACTACTGGCAATTGGTACAAAAGAAGAAATGATGCCAATG
ATGAGTCCTGAACTTTATGATACAATAAGCTCTAAACATATAGGCTATACAGATAAACAGTATGACGCATTACAAGATCT
ATTAATTGATATTTCATCAAGACATCATAAATTAAAACTGAACCGTAACCATATCATTGGTCACCAGGAATATGCTCCAG
AACGAAAGACAGATCCGGGCGCGCTCTTTGATTGGGAACGGTTAATGAGTGATTTCGAGAATTAG

Upstream 100 bases:

>100_bases
ACAGTTGCTAATGATTCTATTAACATTCTATAACATGAAAAATTAAACAAAATCTTCTTTTTTCCGATAGTGAAAGTAGA
TCACTTTGGAGGATAGAAAA

Downstream 100 bases:

>100_bases
AGAATGGAGGTATAGTACAATTTGTTTCGAGTTCTTATAATTGAAGATGATCAAACATTGTTAAGTGAGTTAATCAGCCA
GTTAAGTCAATGGGATTATG

Product: hypothetical protein

Products: NA

Alternate protein names: N-acetylmuramoyl-L-alanine amidase [H]

Number of amino acids: Translated: 234; Mature: 234

Protein sequence:

>234_residues
MFNLWKISFVIVALFFLVACKQEVTKDTLEVAAKEEKVESVEVNFEEKMIDMYLPEENSEPRDSLVTHVMLHFSSNVIEK
PEDPFQVEDIYKVFHDYGVSTHYVIDRTGEIYRFVSEDRNAYHAGEGKIEGVPKYENNMNRYSIGIELLAIGTKEEMMPM
MSPELYDTISSKHIGYTDKQYDALQDLLIDISSRHHKLKLNRNHIIGHQEYAPERKTDPGALFDWERLMSDFEN

Sequences:

>Translated_234_residues
MFNLWKISFVIVALFFLVACKQEVTKDTLEVAAKEEKVESVEVNFEEKMIDMYLPEENSEPRDSLVTHVMLHFSSNVIEK
PEDPFQVEDIYKVFHDYGVSTHYVIDRTGEIYRFVSEDRNAYHAGEGKIEGVPKYENNMNRYSIGIELLAIGTKEEMMPM
MSPELYDTISSKHIGYTDKQYDALQDLLIDISSRHHKLKLNRNHIIGHQEYAPERKTDPGALFDWERLMSDFEN
>Mature_234_residues
MFNLWKISFVIVALFFLVACKQEVTKDTLEVAAKEEKVESVEVNFEEKMIDMYLPEENSEPRDSLVTHVMLHFSSNVIEK
PEDPFQVEDIYKVFHDYGVSTHYVIDRTGEIYRFVSEDRNAYHAGEGKIEGVPKYENNMNRYSIGIELLAIGTKEEMMPM
MSPELYDTISSKHIGYTDKQYDALQDLLIDISSRHHKLKLNRNHIIGHQEYAPERKTDPGALFDWERLMSDFEN

Specific function: Involved in both cell wall peptidoglycans recycling and beta-lactamase induction. Specifically cleaves the amide bond between the lactyl group of N-acetylmuramic acid and the alpha- amino group of the L-alanine in degradation products containing an anhydr

COG id: COG3023

COG function: function code V; Negative regulator of beta-lactamase expression

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1786300, Length=130, Percent_Identity=35.3846153846154, Blast_Score=71, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502 [H]

Pfam domain/function: PF01510 Amidase_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 27338; Mature: 27338

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFNLWKISFVIVALFFLVACKQEVTKDTLEVAAKEEKVESVEVNFEEKMIDMYLPEENSE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCHHHHHHHHCCCCCCCC
PRDSLVTHVMLHFSSNVIEKPEDPFQVEDIYKVFHDYGVSTHYVIDRTGEIYRFVSEDRN
HHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHCCCC
AYHAGEGKIEGVPKYENNMNRYSIGIELLAIGTKEEMMPMMSPELYDTISSKHIGYTDKQ
CEECCCCCCCCCCCCCCCCCEEEEEEEEEEECCHHHHHHHCCHHHHHHHHCCCCCCCCHH
YDALQDLLIDISSRHHKLKLNRNHIIGHQEYAPERKTDPGALFDWERLMSDFEN
HHHHHHHHHHHHCCCEEEEECCCEECCCHHHCCCCCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MFNLWKISFVIVALFFLVACKQEVTKDTLEVAAKEEKVESVEVNFEEKMIDMYLPEENSE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCHHHHHHHHCCCCCCCC
PRDSLVTHVMLHFSSNVIEKPEDPFQVEDIYKVFHDYGVSTHYVIDRTGEIYRFVSEDRN
HHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHCCCC
AYHAGEGKIEGVPKYENNMNRYSIGIELLAIGTKEEMMPMMSPELYDTISSKHIGYTDKQ
CEECCCCCCCCCCCCCCCCCEEEEEEEEEEECCHHHHHHHCCHHHHHHHHCCCCCCCCHH
YDALQDLLIDISSRHHKLKLNRNHIIGHQEYAPERKTDPGALFDWERLMSDFEN
HHHHHHHHHHHHCCCEEEEECCCEECCCHHHCCCCCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8383940 [H]