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The map label for this gene is livJ [C]

Identifier: 23098142

GI number: 23098142

Start: 722466

End: 723665

Strand: Direct

Name: livJ [C]

Synonym: OB0687

Alternate gene names: 23098142

Gene position: 722466-723665 (Clockwise)

Preceding gene: 23098141

Following gene: 23098143

Centisome position: 19.9

GC content: 37.92

Gene sequence:

>1200_bases
ATGAGAAAAAACAAAATTTTCTTAAGTTTGATTTCCTTTTTGCTTATCGTGATTTTAACGGGATGTATTGACGGTAATAC
ATCCAATAATAACAGTTCAGACTCTAGTACGAAGGCAGAGACAGAAGAAAAGGTTGTTAATATTGGATATACGGGTCCTT
TAAGTGGTTCTGCTGCATTATATGGGGAAAATACTTTAAATGGTTTAGAAATGGCAGCAGAAGAAATTAATGAAGAGGGA
TTTGAGGTTAATGGTGAAACATATAAATTAAATATTGTTTCTCTTGATGATAAATATTTACCAAATGAGTCAGCTTCTAA
TGCCAAACGATTAGTGCAAGAAAACGCTACACCAATTATTTATACCCCACATAGTGGTGGTATTGCAGCGCTTCAAGTAT
TTAATGAGCAAGAGAATTTTATTATTGGTGCATATTCTAGTGAACCTGCAATTACAGAACAGGGAAATGAATTAACCGTA
AGAATTCCACCAAGTTATCACGGATATGTCGAACCTTTTACAACATATTCGATGGAGCGGTTTGGAAATAAATTAGCAGT
GATTCCTCCAGTAACACAGTACGGTCAAGATTGGGCAGAGGAATTACTGCCTCATTGGGAGGAACAAGGGGGAGAGGTAG
TTCACAAGGCATCAGTAGATTTTGCAAAAGAAACGGATTTTTATACTTTATTGACCAACGCTTTAGAATCAGATCCTGAT
GTAATCTTCCTCGGAGGACCGTCAGAACCAACAGCGAATGTGGTTAATCAGGCGAGACAGCTTGGATTTGAAGGTGGATT
TATTATTATGGACCAAGCCAAATTAGATGAGATGAAACGAATTACAGAGACGTATGACGTCTTGGAAGGTGCGATTGGAA
CGATGCCATTAGTCGAAGCGGATTATCCTGGCGTACCAGCATTTGTAGAGAAATATACAGAAGAACATGGAATTGAACCG
GGATCAGAAGCTGGTTTTCATTATGTGAGTTTGTATATTTTTGTTGAAGCAATGAAAGCAGCAGGAAATGTAGAGGATGC
TACCGTAATCCGAGAGCATATACAGGATGGTTTAGATGCTTTGCCAGAAGATAAACAAGTATATGTCATCCCAAGTATAG
ACGAGAATGGTGCATTCGAAATTGTTACGAGAGTCAGTGCAGTAGAAGATGGAGAAATAATAGGTATCCCAATCGATTAA

Upstream 100 bases:

>100_bases
TAGTAGAAGGTCCAAAAGAAGAGTTGCTTTCAAATGAAGAAGTTAGAAAGGCTTATATTGGGGCTTAAAACTCAATATCT
TAAAAATAGGAGGAATTTTA

Downstream 100 bases:

>100_bases
CCTTTTCTAATCATTTTTAAAACAATAAAACTTGGCATTTTTCACTGCCAAGTTTTACTTCTTTTTTCCATAAGAAGCAA
TGATTATGTACGGAACAGGA

Product: branched-chain amino acid ABC transporter amino acid-binding protein

Products: ADP; phosphate; L-leucine [Cytoplasm]; ADP; L-valine [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 399; Mature: 399

Protein sequence:

>399_residues
MRKNKIFLSLISFLLIVILTGCIDGNTSNNNSSDSSTKAETEEKVVNIGYTGPLSGSAALYGENTLNGLEMAAEEINEEG
FEVNGETYKLNIVSLDDKYLPNESASNAKRLVQENATPIIYTPHSGGIAALQVFNEQENFIIGAYSSEPAITEQGNELTV
RIPPSYHGYVEPFTTYSMERFGNKLAVIPPVTQYGQDWAEELLPHWEEQGGEVVHKASVDFAKETDFYTLLTNALESDPD
VIFLGGPSEPTANVVNQARQLGFEGGFIIMDQAKLDEMKRITETYDVLEGAIGTMPLVEADYPGVPAFVEKYTEEHGIEP
GSEAGFHYVSLYIFVEAMKAAGNVEDATVIREHIQDGLDALPEDKQVYVIPSIDENGAFEIVTRVSAVEDGEIIGIPID

Sequences:

>Translated_399_residues
MRKNKIFLSLISFLLIVILTGCIDGNTSNNNSSDSSTKAETEEKVVNIGYTGPLSGSAALYGENTLNGLEMAAEEINEEG
FEVNGETYKLNIVSLDDKYLPNESASNAKRLVQENATPIIYTPHSGGIAALQVFNEQENFIIGAYSSEPAITEQGNELTV
RIPPSYHGYVEPFTTYSMERFGNKLAVIPPVTQYGQDWAEELLPHWEEQGGEVVHKASVDFAKETDFYTLLTNALESDPD
VIFLGGPSEPTANVVNQARQLGFEGGFIIMDQAKLDEMKRITETYDVLEGAIGTMPLVEADYPGVPAFVEKYTEEHGIEP
GSEAGFHYVSLYIFVEAMKAAGNVEDATVIREHIQDGLDALPEDKQVYVIPSIDENGAFEIVTRVSAVEDGEIIGIPID
>Mature_399_residues
MRKNKIFLSLISFLLIVILTGCIDGNTSNNNSSDSSTKAETEEKVVNIGYTGPLSGSAALYGENTLNGLEMAAEEINEEG
FEVNGETYKLNIVSLDDKYLPNESASNAKRLVQENATPIIYTPHSGGIAALQVFNEQENFIIGAYSSEPAITEQGNELTV
RIPPSYHGYVEPFTTYSMERFGNKLAVIPPVTQYGQDWAEELLPHWEEQGGEVVHKASVDFAKETDFYTLLTNALESDPD
VIFLGGPSEPTANVVNQARQLGFEGGFIIMDQAKLDEMKRITETYDVLEGAIGTMPLVEADYPGVPAFVEKYTEEHGIEP
GSEAGFHYVSLYIFVEAMKAAGNVEDATVIREHIQDGLDALPEDKQVYVIPSIDENGAFEIVTRVSAVEDGEIIGIPID

Specific function: Component of an amino-acid transport system (Potential) [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI48994941, Length=253, Percent_Identity=23.7154150197628, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: 10140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 8822 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal med

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 43652; Mature: 43652

Theoretical pI: Translated: 3.97; Mature: 3.97

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKNKIFLSLISFLLIVILTGCIDGNTSNNNSSDSSTKAETEEKVVNIGYTGPLSGSAAL
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHCHHHEEEECCCCCCCCCEEE
YGENTLNGLEMAAEEINEEGFEVNGETYKLNIVSLDDKYLPNESASNAKRLVQENATPII
ECCCCCHHHHHHHHHHCCCCCEECCCEEEEEEEEECCCCCCCCCHHHHHHHHHCCCCCEE
YTPHSGGIAALQVFNEQENFIIGAYSSEPAITEQGNELTVRIPPSYHGYVEPFTTYSMER
ECCCCCCEEEEEEECCCCCEEEEEECCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHH
FGNKLAVIPPVTQYGQDWAEELLPHWEEQGGEVVHKASVDFAKETDFYTLLTNALESDPD
HCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHCCCHHHHHHHHHCCCCC
VIFLGGPSEPTANVVNQARQLGFEGGFIIMDQAKLDEMKRITETYDVLEGAIGTMPLVEA
EEEECCCCCCHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHCCCCEEEC
DYPGVPAFVEKYTEEHGIEPGSEAGFHYVSLYIFVEAMKAAGNVEDATVIREHIQDGLDA
CCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
LPEDKQVYVIPSIDENGAFEIVTRVSAVEDGEIIGIPID
CCCCCEEEEEECCCCCCCEEEEEEHHHCCCCCEEEEECC
>Mature Secondary Structure
MRKNKIFLSLISFLLIVILTGCIDGNTSNNNSSDSSTKAETEEKVVNIGYTGPLSGSAAL
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHCHHHEEEECCCCCCCCCEEE
YGENTLNGLEMAAEEINEEGFEVNGETYKLNIVSLDDKYLPNESASNAKRLVQENATPII
ECCCCCHHHHHHHHHHCCCCCEECCCEEEEEEEEECCCCCCCCCHHHHHHHHHCCCCCEE
YTPHSGGIAALQVFNEQENFIIGAYSSEPAITEQGNELTVRIPPSYHGYVEPFTTYSMER
ECCCCCCEEEEEEECCCCCEEEEEECCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHH
FGNKLAVIPPVTQYGQDWAEELLPHWEEQGGEVVHKASVDFAKETDFYTLLTNALESDPD
HCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHCCCHHHHHHHHHCCCCC
VIFLGGPSEPTANVVNQARQLGFEGGFIIMDQAKLDEMKRITETYDVLEGAIGTMPLVEA
EEEECCCCCCHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHCCCCEEEC
DYPGVPAFVEKYTEEHGIEPGSEAGFHYVSLYIFVEAMKAAGNVEDATVIREHIQDGLDA
CCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
LPEDKQVYVIPSIDENGAFEIVTRVSAVEDGEIIGIPID
CCCCCEEEEEECCCCCCCEEEEEEHHHCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O; ATP; L-valine [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA