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The map label for this gene is opuD [H]

Identifier: 23098115

GI number: 23098115

Start: 695047

End: 696597

Strand: Direct

Name: opuD [H]

Synonym: OB0660

Alternate gene names: 23098115

Gene position: 695047-696597 (Clockwise)

Preceding gene: 23098113

Following gene: 23098116

Centisome position: 19.14

GC content: 38.1

Gene sequence:

>1551_bases
TTGAAAAATATAAAGAAACAAAATCAAACGATAGATAAACCAGTACTATTTCTAAGTGGAGGGGCATTACTTGCCTTTGT
GATTTTAGCACTGATAAATAAAGAAATGGTTACAGATGCGGTTAATACAATGTTTAATTGGTCGGTAACGTACTTTGGCT
CCATTTATCAAGTTTTAATGGTCGGTACCTTAATCATTGCATTAATTCTTGGGTATTCCAAGTTTGGTAAAATCCGATTA
GGAAAACTAGAAAAACCAGAAATTGGAACATTTAAATGGATCTCCATTATTATGTGTACGCTACTCGCAGCTGGCGGTGT
TTTTTGGGCAGCTGCTGAACCGTTAAGTCACTTTTTAACGGTACCTCCTCATTTCACAGGTATTGAGGCAGGAACGCCAG
AAGCAATAGTACCAGCACTTGTTACAAGTTTTGTCGATTGGGGCTTTGTTTCCTGGGCAATTTTAGGAACATTAGGTACG
ATTGTATTGATGTATTCCCATTATCATAGAAATGCACCATTAAAACCACGTTCCTTATTGTATCCGATATTTGGAGATAA
AATCATGAAAAAAAGTGCACTAGGAACGTTCGTTGATGCATTTTCTATTGTATCCGTAGCAGCGGGTACGATTGGTCCGA
TTGGATTTCTTGGTTTACAAGCTGGTTATGGGTTAACTGCATTGTTTGGTTTACCAAATAATTTATTAATGCAATGTATT
ATCGTTGCAGTAATTGTTCTAGCAGCGGGGATTTCTGCCGCAACAGGTATTCATAAAGGAATACAAATTTTAAGTAGCTA
TAATATTATTCTTGCTGTTGGATTAATGATTGGTGTCCTTATCTTAGGACCAGCATTATTTATCTTTGATAACTATGTGC
AATCATTTGGAATGTATGTTCAAGACTTCTTAACGTTGAATACGTTCCGAAGTGATGGTGCTTGGCTTGGGTTATGGACA
ATCTTTTTCTTTGCTTGGTTTATCGGTTATGGACCAATGATGGCAATGTTTGTTAGTCGTATATCCCGTGGCCGTACGAT
TCGTGAACTAGTAACTGCTGTTGCAGTAATTGCACCTGTTGTCACAACATTTTGGTTTACTGTTGTTGGAGGAACAGGAA
TCTTTTCAGAAATGAATAGCCCTGGTTCTGTATCTGATGCATTAAATAGTGCCGGACCTCCAGCAGCTATGATTGCAATT
GCAGAACAGTTGCCTTTTGGAACCATTTTTGGTTTCTTATTCCTTCTAGCAACTATTATATTTGTACTCACGACTACGGA
TTCGATGTCCCTAACGATTTCAATGGCGATAACTGGTCATGGAGATCCAGCTAAGTATTTACGTGTAATTTGGGCGATTC
TCATGGGGGTCGTCGCAATAGTACTGATTACATTAGGTGAAGATAGTGTCGGATCCCTACAATCATTCATCGTAGTTACT
GCGGTTCCGGTTTCCTTACTCATGTTAACCACATTTTGGACTGCACCACGTGTAAGTCGAGAACTAGCTAAAGAACAAAA
AATTGATGAAAAACAATCATATACGAAGTAA

Upstream 100 bases:

>100_bases
GATGTTAATTAAATCTCACATACTACTGGCTCAATAGGGCAAATTAAGGTCAGTAAATCATTATGTGAGTTTTTTTGTGT
TGTATTGAAAGGAGCAGATT

Downstream 100 bases:

>100_bases
TACAAAAAGAGCTGATGTTCCAATCAGCTCTTTTTGTATTTTCTATTTTGGTGAATCCGTAGAATCTTTTTACGAAGTGC
AAGGTATTAATGCTTGCAAT

Product: glycine betaine transporter

Products: Proton [Cytoplasm]; choline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 516; Mature: 516

Protein sequence:

>516_residues
MKNIKKQNQTIDKPVLFLSGGALLAFVILALINKEMVTDAVNTMFNWSVTYFGSIYQVLMVGTLIIALILGYSKFGKIRL
GKLEKPEIGTFKWISIIMCTLLAAGGVFWAAAEPLSHFLTVPPHFTGIEAGTPEAIVPALVTSFVDWGFVSWAILGTLGT
IVLMYSHYHRNAPLKPRSLLYPIFGDKIMKKSALGTFVDAFSIVSVAAGTIGPIGFLGLQAGYGLTALFGLPNNLLMQCI
IVAVIVLAAGISAATGIHKGIQILSSYNIILAVGLMIGVLILGPALFIFDNYVQSFGMYVQDFLTLNTFRSDGAWLGLWT
IFFFAWFIGYGPMMAMFVSRISRGRTIRELVTAVAVIAPVVTTFWFTVVGGTGIFSEMNSPGSVSDALNSAGPPAAMIAI
AEQLPFGTIFGFLFLLATIIFVLTTTDSMSLTISMAITGHGDPAKYLRVIWAILMGVVAIVLITLGEDSVGSLQSFIVVT
AVPVSLLMLTTFWTAPRVSRELAKEQKIDEKQSYTK

Sequences:

>Translated_516_residues
MKNIKKQNQTIDKPVLFLSGGALLAFVILALINKEMVTDAVNTMFNWSVTYFGSIYQVLMVGTLIIALILGYSKFGKIRL
GKLEKPEIGTFKWISIIMCTLLAAGGVFWAAAEPLSHFLTVPPHFTGIEAGTPEAIVPALVTSFVDWGFVSWAILGTLGT
IVLMYSHYHRNAPLKPRSLLYPIFGDKIMKKSALGTFVDAFSIVSVAAGTIGPIGFLGLQAGYGLTALFGLPNNLLMQCI
IVAVIVLAAGISAATGIHKGIQILSSYNIILAVGLMIGVLILGPALFIFDNYVQSFGMYVQDFLTLNTFRSDGAWLGLWT
IFFFAWFIGYGPMMAMFVSRISRGRTIRELVTAVAVIAPVVTTFWFTVVGGTGIFSEMNSPGSVSDALNSAGPPAAMIAI
AEQLPFGTIFGFLFLLATIIFVLTTTDSMSLTISMAITGHGDPAKYLRVIWAILMGVVAIVLITLGEDSVGSLQSFIVVT
AVPVSLLMLTTFWTAPRVSRELAKEQKIDEKQSYTK
>Mature_516_residues
MKNIKKQNQTIDKPVLFLSGGALLAFVILALINKEMVTDAVNTMFNWSVTYFGSIYQVLMVGTLIIALILGYSKFGKIRL
GKLEKPEIGTFKWISIIMCTLLAAGGVFWAAAEPLSHFLTVPPHFTGIEAGTPEAIVPALVTSFVDWGFVSWAILGTLGT
IVLMYSHYHRNAPLKPRSLLYPIFGDKIMKKSALGTFVDAFSIVSVAAGTIGPIGFLGLQAGYGLTALFGLPNNLLMQCI
IVAVIVLAAGISAATGIHKGIQILSSYNIILAVGLMIGVLILGPALFIFDNYVQSFGMYVQDFLTLNTFRSDGAWLGLWT
IFFFAWFIGYGPMMAMFVSRISRGRTIRELVTAVAVIAPVVTTFWFTVVGGTGIFSEMNSPGSVSDALNSAGPPAAMIAI
AEQLPFGTIFGFLFLLATIIFVLTTTDSMSLTISMAITGHGDPAKYLRVIWAILMGVVAIVLITLGEDSVGSLQSFIVVT
AVPVSLLMLTTFWTAPRVSRELAKEQKIDEKQSYTK

Specific function: High-affinity uptake of glycine betaine [H]

COG id: COG1292

COG function: function code M; Choline-glycine betaine transporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the BCCT transporter (TC 2.A.15) family [H]

Homologues:

Organism=Escherichia coli, GI1786506, Length=498, Percent_Identity=29.718875502008, Blast_Score=212, Evalue=4e-56,
Organism=Escherichia coli, GI1788102, Length=436, Percent_Identity=30.5045871559633, Blast_Score=166, Evalue=2e-42,
Organism=Escherichia coli, GI1786224, Length=491, Percent_Identity=26.2729124236253, Blast_Score=160, Evalue=2e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000060
- InterPro:   IPR018093 [H]

Pfam domain/function: PF02028 BCCT [H]

EC number: NA

Molecular weight: Translated: 55712; Mature: 55712

Theoretical pI: Translated: 9.31; Mature: 9.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNIKKQNQTIDKPVLFLSGGALLAFVILALINKEMVTDAVNTMFNWSVTYFGSIYQVLM
CCCCCHHCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
VGTLIIALILGYSKFGKIRLGKLEKPEIGTFKWISIIMCTLLAAGGVFWAAAEPLSHFLT
HHHHHHHHHHHHHHCCCEEECCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHEE
VPPHFTGIEAGTPEAIVPALVTSFVDWGFVSWAILGTLGTIVLMYSHYHRNAPLKPRSLL
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHC
YPIFGDKIMKKSALGTFVDAFSIVSVAAGTIGPIGFLGLQAGYGLTALFGLPNNLLMQCI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHCCCHHHHHHHH
IVAVIVLAAGISAATGIHKGIQILSSYNIILAVGLMIGVLILGPALFIFDNYVQSFGMYV
HHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QDFLTLNTFRSDGAWLGLWTIFFFAWFIGYGPMMAMFVSRISRGRTIRELVTAVAVIAPV
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
VTTFWFTVVGGTGIFSEMNSPGSVSDALNSAGPPAAMIAIAEQLPFGTIFGFLFLLATII
HHHHHHHHHCCCHHHHCCCCCCCHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHH
FVLTTTDSMSLTISMAITGHGDPAKYLRVIWAILMGVVAIVLITLGEDSVGSLQSFIVVT
HHHHCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
AVPVSLLMLTTFWTAPRVSRELAKEQKIDEKQSYTK
HHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHCCCC
>Mature Secondary Structure
MKNIKKQNQTIDKPVLFLSGGALLAFVILALINKEMVTDAVNTMFNWSVTYFGSIYQVLM
CCCCCHHCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
VGTLIIALILGYSKFGKIRLGKLEKPEIGTFKWISIIMCTLLAAGGVFWAAAEPLSHFLT
HHHHHHHHHHHHHHCCCEEECCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHEE
VPPHFTGIEAGTPEAIVPALVTSFVDWGFVSWAILGTLGTIVLMYSHYHRNAPLKPRSLL
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHC
YPIFGDKIMKKSALGTFVDAFSIVSVAAGTIGPIGFLGLQAGYGLTALFGLPNNLLMQCI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHCCCHHHHHHHH
IVAVIVLAAGISAATGIHKGIQILSSYNIILAVGLMIGVLILGPALFIFDNYVQSFGMYV
HHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QDFLTLNTFRSDGAWLGLWTIFFFAWFIGYGPMMAMFVSRISRGRTIRELVTAVAVIAPV
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
VTTFWFTVVGGTGIFSEMNSPGSVSDALNSAGPPAAMIAIAEQLPFGTIFGFLFLLATII
HHHHHHHHHCCCHHHHCCCCCCCHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHH
FVLTTTDSMSLTISMAITGHGDPAKYLRVIWAILMGVVAIVLITLGEDSVGSLQSFIVVT
HHHHCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
AVPVSLLMLTTFWTAPRVSRELAKEQKIDEKQSYTK
HHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; choline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + choline [Periplasm] = Proton [Cytoplasm] + choline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8752321; 9387221; 9384377 [H]