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The map label for this gene is pcpB [H]

Identifier: 23098093

GI number: 23098093

Start: 667198

End: 668688

Strand: Direct

Name: pcpB [H]

Synonym: OB0638

Alternate gene names: 23098093

Gene position: 667198-668688 (Clockwise)

Preceding gene: 23098092

Following gene: 23098094

Centisome position: 18.38

GC content: 32.8

Gene sequence:

>1491_bases
ATGAAAGAGGAAGTATTAATTATTGGAGCGGGTCCTACAGGTTTAGCTATGGCAATTGGATTAGAAAAACAAGGTGTTTC
ATTTCGAATTGTAGATAAAAAATCAGCACCAGGTACTGCCTCAAGAGCAATGGCGGTTCAAGCTAGGGTGTTAGAATCCT
ATTATCAATTTGGGATTGATCAAGAAATCATTCAAAACGGTATTATTATTGACCAAACTAATATTATCAAAGATAGAGAA
AAAATAGTAAGTATACCATTTGGAAGTATAGGTGGTGAAATTAGTCCCTATCCTTATGTATTAAGTATTCCTCAAGATGT
ACATGAAAGAATACTTGTTAAGTATTTAGAGTCCAAAAATATATCAATTGAATGGGATACCGAAGTTACGAATTTAATAG
AAGAAGAGGATTATGTTGATGTTATATTAAATGAAGGCGATGCCGAAGAAAACCAACACTTTAAATATATATGTGGGTGT
GATGGTGCTCATAGTATTGTTAGAAAACAGGCAAAGATAGATTTCCCAGGAGATACTTATAATGAGAGATTTTTTGTTGC
TGATGTAGAAATTGAAGAAGATCAAAATGGTATGAGTGTTGGTTTTAGAAAAGAAGCGTTTTGTTTAGCTATGAACATTT
GTCATTCTAGCAGTGTGCGTTTAATAGGGATTATTCCTAAAGATTTTAAAGGAGAAGAACCTGAGACATTTCATCCGATT
ATCCCTTATGCAGAAAAGATCTTGCCACTAAAAATAAGGAGTGTGAACTGGTATTCGCCTTATAAAATTCATTGCCGGGT
TGCAGAGAAATTTAAAAGTGGAAGATTGTTCTTGTTAGGTGATGCAGGACATATTCATAGTCCGGCAGGTGGTCAAGGTA
TGAATACAGGTATAATGGATGCTTTTAATCTTTCTTGGAAGTTAGCATTTGTATTGAAAGGGAAATTAAATGAAGATGTT
TTAAGAACCTATGAAGAAGAGCGTATAGCATTTGCAAAATCATTAGTATCAACAACAGATAAAGTATTCCAAAGCGTTGT
GAAGAGTAAAATTCTTAAAAACTTTATCATACCTTATATAGTACCGAAATTAAGTAAAAGTAAGAAGTTAAAGACAAAGC
TCTTCAAGGTCATTTCACAAGTGAAAATTAATTATCGTGATAGTATTCTTAGTAAAGGACGAGTGGGGAAGATTAAAGCT
GGAGATCGTTTGCCATGGATACAAAAAAACAATATCAACAATTATGATGCATTAAAGAGTGTTGATTGGCAAATTCATGT
TTATGGTAAAAATAGTAAGAAACTGAACAGGTTGTCTCAATCTTTTAGTATACCAGTACACTATTATAAATGGTCGAAAG
TCGCTAAGCGAAAGAAAGTTGAAAAGAATTCTATATTTTTAGTTCGACCGGACGGGTATATTGCTGTAGTAATAACGAAA
AAAGAAATAGATGTGCTTAAAAATTACTTGAATAAATTGGGGCTGAAATAA

Upstream 100 bases:

>100_bases
AGTTTATACTATATTAAAGTAGAAATTTAAACATACGTTGAATATAATGCCTGAAGATTAGCTGCATAGAATTACTTCAA
CAGTTTGGAGGGAGACGTTT

Downstream 100 bases:

>100_bases
TGATGCCCTTTTCAATTGAAATAATGATACCGATTTATAGCCTTTCTTGAAACAAGGTTTTGTGTATAAATAATGGAGGT
GGGTCCATGCGGTATATCTT

Product: pentachlorophenol-4-monooxygenase

Products: NA

Alternate protein names: Pentachlorophenol hydroxylase [H]

Number of amino acids: Translated: 496; Mature: 496

Protein sequence:

>496_residues
MKEEVLIIGAGPTGLAMAIGLEKQGVSFRIVDKKSAPGTASRAMAVQARVLESYYQFGIDQEIIQNGIIIDQTNIIKDRE
KIVSIPFGSIGGEISPYPYVLSIPQDVHERILVKYLESKNISIEWDTEVTNLIEEEDYVDVILNEGDAEENQHFKYICGC
DGAHSIVRKQAKIDFPGDTYNERFFVADVEIEEDQNGMSVGFRKEAFCLAMNICHSSSVRLIGIIPKDFKGEEPETFHPI
IPYAEKILPLKIRSVNWYSPYKIHCRVAEKFKSGRLFLLGDAGHIHSPAGGQGMNTGIMDAFNLSWKLAFVLKGKLNEDV
LRTYEEERIAFAKSLVSTTDKVFQSVVKSKILKNFIIPYIVPKLSKSKKLKTKLFKVISQVKINYRDSILSKGRVGKIKA
GDRLPWIQKNNINNYDALKSVDWQIHVYGKNSKKLNRLSQSFSIPVHYYKWSKVAKRKKVEKNSIFLVRPDGYIAVVITK
KEIDVLKNYLNKLGLK

Sequences:

>Translated_496_residues
MKEEVLIIGAGPTGLAMAIGLEKQGVSFRIVDKKSAPGTASRAMAVQARVLESYYQFGIDQEIIQNGIIIDQTNIIKDRE
KIVSIPFGSIGGEISPYPYVLSIPQDVHERILVKYLESKNISIEWDTEVTNLIEEEDYVDVILNEGDAEENQHFKYICGC
DGAHSIVRKQAKIDFPGDTYNERFFVADVEIEEDQNGMSVGFRKEAFCLAMNICHSSSVRLIGIIPKDFKGEEPETFHPI
IPYAEKILPLKIRSVNWYSPYKIHCRVAEKFKSGRLFLLGDAGHIHSPAGGQGMNTGIMDAFNLSWKLAFVLKGKLNEDV
LRTYEEERIAFAKSLVSTTDKVFQSVVKSKILKNFIIPYIVPKLSKSKKLKTKLFKVISQVKINYRDSILSKGRVGKIKA
GDRLPWIQKNNINNYDALKSVDWQIHVYGKNSKKLNRLSQSFSIPVHYYKWSKVAKRKKVEKNSIFLVRPDGYIAVVITK
KEIDVLKNYLNKLGLK
>Mature_496_residues
MKEEVLIIGAGPTGLAMAIGLEKQGVSFRIVDKKSAPGTASRAMAVQARVLESYYQFGIDQEIIQNGIIIDQTNIIKDRE
KIVSIPFGSIGGEISPYPYVLSIPQDVHERILVKYLESKNISIEWDTEVTNLIEEEDYVDVILNEGDAEENQHFKYICGC
DGAHSIVRKQAKIDFPGDTYNERFFVADVEIEEDQNGMSVGFRKEAFCLAMNICHSSSVRLIGIIPKDFKGEEPETFHPI
IPYAEKILPLKIRSVNWYSPYKIHCRVAEKFKSGRLFLLGDAGHIHSPAGGQGMNTGIMDAFNLSWKLAFVLKGKLNEDV
LRTYEEERIAFAKSLVSTTDKVFQSVVKSKILKNFIIPYIVPKLSKSKKLKTKLFKVISQVKINYRDSILSKGRVGKIKA
GDRLPWIQKNNINNYDALKSVDWQIHVYGKNSKKLNRLSQSFSIPVHYYKWSKVAKRKKVEKNSIFLVRPDGYIAVVITK
KEIDVLKNYLNKLGLK

Specific function: Dechlorination of pentachlorophenol to tetrachlorohydroquinone. Removes also hydrogen and nitro, amino, and cyano groups from benzene ring at the para position in relation to the hydroxyl of phenol [H]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pheA/tfdB FAD monooxygenase family [H]

Homologues:

Organism=Escherichia coli, GI1786543, Length=351, Percent_Identity=23.6467236467236, Blast_Score=103, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: =1.14.13.50 [H]

Molecular weight: Translated: 56244; Mature: 56244

Theoretical pI: Translated: 9.74; Mature: 9.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEEVLIIGAGPTGLAMAIGLEKQGVSFRIVDKKSAPGTASRAMAVQARVLESYYQFGID
CCCCEEEEECCCCCEEEEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCC
QEIIQNGIIIDQTNIIKDREKIVSIPFGSIGGEISPYPYVLSIPQDVHERILVKYLESKN
HHHHHCCEEEECCHHHCCCHHEEECCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHCCC
ISIEWDTEVTNLIEEEDYVDVILNEGDAEENQHFKYICGCDGAHSIVRKQAKIDFPGDTY
CEEEECCHHHHHHCCCCCEEEEECCCCCCCCCCEEEEECCCCHHHHHHHHCCCCCCCCCC
NERFFVADVEIEEDQNGMSVGFRKEAFCLAMNICHSSSVRLIGIIPKDFKGEEPETFHPI
CCEEEEEEEEEECCCCCCEECCCHHHHEEEEEHHCCCCEEEEEECCCCCCCCCCCCCCCC
IPYAEKILPLKIRSVNWYSPYKIHCRVAEKFKSGRLFLLGDAGHIHSPAGGQGMNTGIMD
CCHHHCEEEEEEEEECCCCCEEEEEEEHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCE
AFNLSWKLAFVLKGKLNEDVLRTYEEERIAFAKSLVSTTDKVFQSVVKSKILKNFIIPYI
EECCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VPKLSKSKKLKTKLFKVISQVKINYRDSILSKGRVGKIKAGDRLPWIQKNNINNYDALKS
HCCCCCCHHHHHHHHHHHHHHHCCHHHHHHCCCCCCEEECCCCCCCEECCCCCCCHHHCC
VDWQIHVYGKNSKKLNRLSQSFSIPVHYYKWSKVAKRKKVEKNSIFLVRPDGYIAVVITK
CCEEEEEEECCCHHHHHHHHHCCCCEEEEHHHHHHHHHCCCCCCEEEECCCCEEEEEEEC
KEIDVLKNYLNKLGLK
HHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKEEVLIIGAGPTGLAMAIGLEKQGVSFRIVDKKSAPGTASRAMAVQARVLESYYQFGID
CCCCEEEEECCCCCEEEEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCC
QEIIQNGIIIDQTNIIKDREKIVSIPFGSIGGEISPYPYVLSIPQDVHERILVKYLESKN
HHHHHCCEEEECCHHHCCCHHEEECCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHCCC
ISIEWDTEVTNLIEEEDYVDVILNEGDAEENQHFKYICGCDGAHSIVRKQAKIDFPGDTY
CEEEECCHHHHHHCCCCCEEEEECCCCCCCCCCEEEEECCCCHHHHHHHHCCCCCCCCCC
NERFFVADVEIEEDQNGMSVGFRKEAFCLAMNICHSSSVRLIGIIPKDFKGEEPETFHPI
CCEEEEEEEEEECCCCCCEECCCHHHHEEEEEHHCCCCEEEEEECCCCCCCCCCCCCCCC
IPYAEKILPLKIRSVNWYSPYKIHCRVAEKFKSGRLFLLGDAGHIHSPAGGQGMNTGIMD
CCHHHCEEEEEEEEECCCCCEEEEEEEHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCE
AFNLSWKLAFVLKGKLNEDVLRTYEEERIAFAKSLVSTTDKVFQSVVKSKILKNFIIPYI
EECCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VPKLSKSKKLKTKLFKVISQVKINYRDSILSKGRVGKIKAGDRLPWIQKNNINNYDALKS
HCCCCCCHHHHHHHHHHHHHHHCCHHHHHHCCCCCCEEECCCCCCCEECCCCCCCHHHCC
VDWQIHVYGKNSKKLNRLSQSFSIPVHYYKWSKVAKRKKVEKNSIFLVRPDGYIAVVITK
CCEEEEEEECCCHHHHHHHHHCCCCEEEEHHHHHHHHHCCCCCCEEEECCCCEEEEEEEC
KEIDVLKNYLNKLGLK
HHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7678243; 12169590 [H]