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The map label for this gene is sigB [H]

Identifier: 23098085

GI number: 23098085

Start: 655820

End: 656611

Strand: Direct

Name: sigB [H]

Synonym: OB0630

Alternate gene names: 23098085

Gene position: 655820-656611 (Clockwise)

Preceding gene: 23098084

Following gene: 23098086

Centisome position: 18.06

GC content: 36.99

Gene sequence:

>792_bases
ATGACGACCAAATCTCAACCACACAATAAAGGGGGAGATGAGGTTTACCAATGGATTGAATATCTACAGAAAGATCCAAC
TAATGAAGAAATACAAGAGAAAATTGTCTTGAAGTATCAAGATTTAGTGGGGTCCATCGCGAGAAAGTATTCCAAAAATA
GTGCGATTCATGAAGATTTAGTACAAGTTGGAATGATTGGTTTGCTGGCAGCGGTAAGAAGATATGATCCAACCTATGGT
AAGTCATTTGAATCCTTTGCAATACCAACAATTATTGGTGAAATTAAACGCTTTATACGTGATAAAACGTGGAGTGTACA
CGTGCCACGGCGTATTAAAGAATTAGGACCTAAAATTAGAAAAGGGGTAGATGAATTAACTACCGATAACCAGAAGTCAC
CTACAGTGGCGGAAATAGCAAAATATATTGGAACTTCAGAAGAAGAAGTTTTAGAAACGATGGAAATGGGGCAGAGCTAT
AAAGCATTATCCGTTGACCGGAAAATAGAAGCTGATTCTGACGGAAGTACAGTAGCTATTTTAGATCTAGTAGGTAATAG
TGAAGACGGTTATGATAATGTAGAACAACGTATGATGCTCGAAAAAATACTTCCGATCTTGTCAGAAAGAGAACAACAAA
TATTAGAATGTACGTATTTTAAAAATATGAGTCAAAAGGAAACAGGAGAACTTCTTGGTATATCTCAAATGCACGTTTCT
AGACTACAACGTCGTTCTTTAAGAAAGCTTAGGGAAGCGATTCAGTCGGAGAGTACGGAGGTTTTAGATTGA

Upstream 100 bases:

>100_bases
TTTCTTATTGAAGCTCTAATGGACGATGTAAAAATCAATAATCAATATGGCGTAATGATTATGATGTCTAAATATATAGC
AGAAGAAGAGGTGGACATGG

Downstream 100 bases:

>100_bases
TTTCTGGAGAACATGTAGATGTCTCTGTATTTCAAGAACCAAAAAAAGGGAATTATGAATGTGGAGATAGCTATTTTTAT
ATAGAGTTGGAAAATGAATT

Product: RNA polymerase sigma factor SigB

Products: NA

Alternate protein names: General stress protein 84; GSP84; Sigma-37 [H]

Number of amino acids: Translated: 263; Mature: 262

Protein sequence:

>263_residues
MTTKSQPHNKGGDEVYQWIEYLQKDPTNEEIQEKIVLKYQDLVGSIARKYSKNSAIHEDLVQVGMIGLLAAVRRYDPTYG
KSFESFAIPTIIGEIKRFIRDKTWSVHVPRRIKELGPKIRKGVDELTTDNQKSPTVAEIAKYIGTSEEEVLETMEMGQSY
KALSVDRKIEADSDGSTVAILDLVGNSEDGYDNVEQRMMLEKILPILSEREQQILECTYFKNMSQKETGELLGISQMHVS
RLQRRSLRKLREAIQSESTEVLD

Sequences:

>Translated_263_residues
MTTKSQPHNKGGDEVYQWIEYLQKDPTNEEIQEKIVLKYQDLVGSIARKYSKNSAIHEDLVQVGMIGLLAAVRRYDPTYG
KSFESFAIPTIIGEIKRFIRDKTWSVHVPRRIKELGPKIRKGVDELTTDNQKSPTVAEIAKYIGTSEEEVLETMEMGQSY
KALSVDRKIEADSDGSTVAILDLVGNSEDGYDNVEQRMMLEKILPILSEREQQILECTYFKNMSQKETGELLGISQMHVS
RLQRRSLRKLREAIQSESTEVLD
>Mature_262_residues
TTKSQPHNKGGDEVYQWIEYLQKDPTNEEIQEKIVLKYQDLVGSIARKYSKNSAIHEDLVQVGMIGLLAAVRRYDPTYGK
SFESFAIPTIIGEIKRFIRDKTWSVHVPRRIKELGPKIRKGVDELTTDNQKSPTVAEIAKYIGTSEEEVLETMEMGQSYK
ALSVDRKIEADSDGSTVAILDLVGNSEDGYDNVEQRMMLEKILPILSEREQQILECTYFKNMSQKETGELLGISQMHVSR
LQRRSLRKLREAIQSESTEVLD

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. Sigma B is not essential for sporulation; rather it is required for maximal expression of ctc and csbA which are transcr

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. SigB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789098, Length=230, Percent_Identity=26.0869565217391, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1788231, Length=202, Percent_Identity=26.2376237623762, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1789871, Length=241, Percent_Identity=28.6307053941909, Blast_Score=71, Evalue=9e-14,
Organism=Escherichia coli, GI1789448, Length=225, Percent_Identity=24, Blast_Score=64, Evalue=8e-12,

Paralogues:

None

Copy number: <10 (log phase) 250 (stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014288
- InterPro:   IPR014322
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 30047; Mature: 29915

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTKSQPHNKGGDEVYQWIEYLQKDPTNEEIQEKIVLKYQDLVGSIARKYSKNSAIHEDL
CCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
VQVGMIGLLAAVRRYDPTYGKSFESFAIPTIIGEIKRFIRDKTWSVHVPRRIKELGPKIR
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEECCHHHHHHCHHHH
KGVDELTTDNQKSPTVAEIAKYIGTSEEEVLETMEMGQSYKALSVDRKIEADSDGSTVAI
HHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCEEEE
LDLVGNSEDGYDNVEQRMMLEKILPILSEREQQILECTYFKNMSQKETGELLGISQMHVS
EEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
RLQRRSLRKLREAIQSESTEVLD
HHHHHHHHHHHHHHHCCCHHCCC
>Mature Secondary Structure 
TTKSQPHNKGGDEVYQWIEYLQKDPTNEEIQEKIVLKYQDLVGSIARKYSKNSAIHEDL
CCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
VQVGMIGLLAAVRRYDPTYGKSFESFAIPTIIGEIKRFIRDKTWSVHVPRRIKELGPKIR
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEECCHHHHHHCHHHH
KGVDELTTDNQKSPTVAEIAKYIGTSEEEVLETMEMGQSYKALSVDRKIEADSDGSTVAI
HHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCEEEE
LDLVGNSEDGYDNVEQRMMLEKILPILSEREQQILECTYFKNMSQKETGELLGISQMHVS
EEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
RLQRRSLRKLREAIQSESTEVLD
HHHHHHHHHHHHHHHCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3016731; 3027048; 2170324; 9384377; 9298659 [H]