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The map label for this gene is rsbW

Identifier: 23098084

GI number: 23098084

Start: 655372

End: 655848

Strand: Direct

Name: rsbW

Synonym: OB0629

Alternate gene names: 23098084

Gene position: 655372-655848 (Clockwise)

Preceding gene: 23098083

Following gene: 23098085

Centisome position: 18.05

GC content: 36.06

Gene sequence:

>477_bases
ATGAGTAATTATGATTATATTGAAATGAAAGTTCCAGCAAAACCTGAATATGTTGGTGTTGCGCGCCTTACATTATCGGG
AGTTGCTAACCGTATGGGCTTTTCGTACGAATCGATAGAGGATCTAAAAGTTGCGGTATCTGAAGCAATTACAAATGCGG
TAAATCATGCTTACAATGATAATGAATCCGGTGAGATTAACATTGGTTTTGGTATATATGAGGATCATATTGAAATTATG
ATCGCTGACCGTGGTGAAAGTTTTGATTTAGAAAAAATTAAACAAGAAACAGGGCCATACCACCCAGAAGAACCAGTAGA
AAAACTAAGAGAAGGTGGGTTTGGGTTATTTCTTATTGAAGCTCTAATGGACGATGTAAAAATCAATAATCAATATGGCG
TAATGATTATGATGTCTAAATATATAGCAGAAGAAGAGGTGGACATGGATGACGACCAAATCTCAACCACACAATAA

Upstream 100 bases:

>100_bases
AATTAATTAATGTTCAAGATAGAGTTTACCGCATCTTTGAAATAACAGGTTTAGATGAAATTATTCAGATTAAAGCGGCG
ATTAGAGGTGTAAATGAATA

Downstream 100 bases:

>100_bases
AGGGGGAGATGAGGTTTACCAATGGATTGAATATCTACAGAAAGATCCAACTAATGAAGAAATACAAGAGAAAATTGTCT
TGAAGTATCAAGATTTAGTG

Product: serine-protein kinase RsbW

Products: NA

Alternate protein names: Anti-sigma-B factor; Sigma-B negative effector rsbW

Number of amino acids: Translated: 158; Mature: 157

Protein sequence:

>158_residues
MSNYDYIEMKVPAKPEYVGVARLTLSGVANRMGFSYESIEDLKVAVSEAITNAVNHAYNDNESGEINIGFGIYEDHIEIM
IADRGESFDLEKIKQETGPYHPEEPVEKLREGGFGLFLIEALMDDVKINNQYGVMIMMSKYIAEEEVDMDDDQISTTQ

Sequences:

>Translated_158_residues
MSNYDYIEMKVPAKPEYVGVARLTLSGVANRMGFSYESIEDLKVAVSEAITNAVNHAYNDNESGEINIGFGIYEDHIEIM
IADRGESFDLEKIKQETGPYHPEEPVEKLREGGFGLFLIEALMDDVKINNQYGVMIMMSKYIAEEEVDMDDDQISTTQ
>Mature_157_residues
SNYDYIEMKVPAKPEYVGVARLTLSGVANRMGFSYESIEDLKVAVSEAITNAVNHAYNDNESGEINIGFGIYEDHIEIMI
ADRGESFDLEKIKQETGPYHPEEPVEKLREGGFGLFLIEALMDDVKINNQYGVMIMMSKYIAEEEVDMDDDQISTTQ

Specific function: Negative regulator of sigma-B activity. Phosphorylates and inactivates its specific antagonist protein, rsbV. Upon phosphorylation of rsbV, rsbW is released and binds to sigma-B, thereby blocking its ability to form an RNA polymerase holoenzyme (E-sigma-B

COG id: COG2172

COG function: function code T; Anti-sigma regulatory factor (Ser/Thr protein kinase)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the anti-sigma-factor family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSBW_OCEIH (Q8CXL7)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_691550.1
- ProteinModelPortal:   Q8CXL7
- SMR:   Q8CXL7
- GeneID:   1016017
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB0629
- NMPDR:   fig|221109.1.peg.634
- HOGENOM:   HBG425195
- OMA:   GEITIVF
- ProtClustDB:   PRK04069
- BioCyc:   OIHE221109:OB0629-MONOMER
- BRENDA:   2.7.11.1
- HAMAP:   MF_00638
- InterPro:   IPR010193
- InterPro:   IPR003594
- Gene3D:   G3DSA:3.30.565.10
- SMART:   SM00387
- TIGRFAMs:   TIGR01924

Pfam domain/function: PF02518 HATPase_c; SSF55874 ATP_bd_ATPase

EC number: =2.7.11.1

Molecular weight: Translated: 17831; Mature: 17700

Theoretical pI: Translated: 3.99; Mature: 3.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
5.7 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNYDYIEMKVPAKPEYVGVARLTLSGVANRMGFSYESIEDLKVAVSEAITNAVNHAYND
CCCCCEEEEECCCCCCEEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCC
NESGEINIGFGIYEDHIEIMIADRGESFDLEKIKQETGPYHPEEPVEKLREGGFGLFLIE
CCCCEEEEECCEEECCEEEEEECCCCCCCHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHH
ALMDDVKINNQYGVMIMMSKYIAEEEVDMDDDQISTTQ
HHHHHHEECCCCCEEEEEHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
SNYDYIEMKVPAKPEYVGVARLTLSGVANRMGFSYESIEDLKVAVSEAITNAVNHAYND
CCCCEEEEECCCCCCEEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCC
NESGEINIGFGIYEDHIEIMIADRGESFDLEKIKQETGPYHPEEPVEKLREGGFGLFLIE
CCCCEEEEECCEEECCEEEEEECCCCCCCHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHH
ALMDDVKINNQYGVMIMMSKYIAEEEVDMDDDQISTTQ
HHHHHHEECCCCCEEEEEHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12235376