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The map label for this gene is ybbF [H]

Identifier: 23098070

GI number: 23098070

Start: 643412

End: 644779

Strand: Direct

Name: ybbF [H]

Synonym: OB0615

Alternate gene names: 23098070

Gene position: 643412-644779 (Clockwise)

Preceding gene: 23098069

Following gene: 23098071

Centisome position: 17.72

GC content: 38.23

Gene sequence:

>1368_bases
ATGAAGAAGGAAGAGAGGATTGCAAGAGAGATATTAGAACATATCGGTGGAAAAGATAATATTCGTTCTTTCACACATTG
CATGACTAGAGTGCGTATAGATATAAAAGAGGATGAGAAAGTGGATAAGTCTGCTCTGAGAGAAATTGAAGGGGTAATGG
GTGTCATTGAAGATGACGTGTTACAAATAGTAGTTGGGCCAGGTACTGTAAACAAAGTAGCTGCTTACATAACAGACATC
ACTGGGATTAAAATTGGTGATAGTTCTGAATCATTTGGTGAAGATGTTGCTAAAGAAACAAAAGAAGGTATAAAGCAAAA
AAATAATACTCCAATGAAAAATCTTCTACGTAAAATTGGAAGTATTTTCATTCCTTTAATCCCAGGTATTGTAGGTTCCG
GGATTATTAATGGTGCAGCAGGATTCTTGAAGAATACTGGTGTTGATCCAACATTAACATGGATGCAACTGTTAACTGTA
TTAGGCGGAAGCATTTTTGGTTATTTAGCCATTGTGGTAGGTTGGAATACAGCTAAAGAATTTGGTGGAACTCCAGTATT
AGGGGCTATTGCAGGTATTATCACAATGAATCCAGCGTTAGCTGATATTTCATTGTTTGGAAACAATCTCACTCCTGGGT
ATGGAGGATTAATTGGTGCCATGTTTGCTGCGTGGCTGATGGTTTTCTTTGAGAAGCAATTCCGAAAGATGATCCCAAGT
GTGGTGGATATAATTTTCACACCAACACTCACTATCCTTACAGTTGGAATGTTGACGTTAGTAGTAGTACAACCAATCGC
CGGTATCCTTACTGAAGGAATTACAGTGGGAATAAATTGGGTGTTAGATGTTGGAGGAGCATTTGCTGGTGCAGTATTAG
CCGGTTTCTTCTTACCATTGGTCATGTTTGGTTTGCATCATGGATTAACTCCTATTCATATGGAATTAATTAATGCATAT
GATGCGACTGCACTATTACCTATACTAGCAATGGCAGGTGCGAGCCAAGTAGGTGCTGCAATTGCAATTTATGTTAAAAC
AAAAAATCAACGTTTGAAAAATGTTATCAAAGGTGGTCTGCCAACTGGCTTCCTCGGAATAGGAGAACCGCTTATTTATG
GGGTTACCTTACCTTTGGGACGTCCATTTATTACTGCTTGTATCGGAGCTGCAGTGGGGGGAGCATTCCAAGCAACGATG
AAGACGGCTTCATCTAGTATAGGTGTTTCTGGCTTACCTTTGATACCGTTAATTGTTGATAATAAATTTTTATTCTACTT
TATTGGGTTGTTAATCACATACACGGCAGGATTTTTAGTAACATATTTCTTCGGATTTAAAGAATCGATGGCAGATAATC
TAAAATAA

Upstream 100 bases:

>100_bases
CCATTGTAATGATTTTATTAAATTGTAATGTAGACGAAGCGATACAACATCTAGATCGGGCAGAAGGATTTATACGTAAA
ACGAAATAGGGGGAGAGACA

Downstream 100 bases:

>100_bases
AATTCCTGTTTAAGAAGGCTCCTGTTAACGGTTGTTTAGGGGTCTTCTTAGTAATAAATCAAGTATAAAGCAAAATGATA
TGGAGGGCTTCAGATGATAG

Product: PTS system sucrose-specific enzyme II BC component

Products: NA

Alternate protein names: Phosphotransferase enzyme IIB component; PTS system EIIB component; Permease IIC component; PTS system EIIC component [H]

Number of amino acids: Translated: 455; Mature: 455

Protein sequence:

>455_residues
MKKEERIAREILEHIGGKDNIRSFTHCMTRVRIDIKEDEKVDKSALREIEGVMGVIEDDVLQIVVGPGTVNKVAAYITDI
TGIKIGDSSESFGEDVAKETKEGIKQKNNTPMKNLLRKIGSIFIPLIPGIVGSGIINGAAGFLKNTGVDPTLTWMQLLTV
LGGSIFGYLAIVVGWNTAKEFGGTPVLGAIAGIITMNPALADISLFGNNLTPGYGGLIGAMFAAWLMVFFEKQFRKMIPS
VVDIIFTPTLTILTVGMLTLVVVQPIAGILTEGITVGINWVLDVGGAFAGAVLAGFFLPLVMFGLHHGLTPIHMELINAY
DATALLPILAMAGASQVGAAIAIYVKTKNQRLKNVIKGGLPTGFLGIGEPLIYGVTLPLGRPFITACIGAAVGGAFQATM
KTASSSIGVSGLPLIPLIVDNKFLFYFIGLLITYTAGFLVTYFFGFKESMADNLK

Sequences:

>Translated_455_residues
MKKEERIAREILEHIGGKDNIRSFTHCMTRVRIDIKEDEKVDKSALREIEGVMGVIEDDVLQIVVGPGTVNKVAAYITDI
TGIKIGDSSESFGEDVAKETKEGIKQKNNTPMKNLLRKIGSIFIPLIPGIVGSGIINGAAGFLKNTGVDPTLTWMQLLTV
LGGSIFGYLAIVVGWNTAKEFGGTPVLGAIAGIITMNPALADISLFGNNLTPGYGGLIGAMFAAWLMVFFEKQFRKMIPS
VVDIIFTPTLTILTVGMLTLVVVQPIAGILTEGITVGINWVLDVGGAFAGAVLAGFFLPLVMFGLHHGLTPIHMELINAY
DATALLPILAMAGASQVGAAIAIYVKTKNQRLKNVIKGGLPTGFLGIGEPLIYGVTLPLGRPFITACIGAAVGGAFQATM
KTASSSIGVSGLPLIPLIVDNKFLFYFIGLLITYTAGFLVTYFFGFKESMADNLK
>Mature_455_residues
MKKEERIAREILEHIGGKDNIRSFTHCMTRVRIDIKEDEKVDKSALREIEGVMGVIEDDVLQIVVGPGTVNKVAAYITDI
TGIKIGDSSESFGEDVAKETKEGIKQKNNTPMKNLLRKIGSIFIPLIPGIVGSGIINGAAGFLKNTGVDPTLTWMQLLTV
LGGSIFGYLAIVVGWNTAKEFGGTPVLGAIAGIITMNPALADISLFGNNLTPGYGGLIGAMFAAWLMVFFEKQFRKMIPS
VVDIIFTPTLTILTVGMLTLVVVQPIAGILTEGITVGINWVLDVGGAFAGAVLAGFFLPLVMFGLHHGLTPIHMELINAY
DATALLPILAMAGASQVGAAIAIYVKTKNQRLKNVIKGGLPTGFLGIGEPLIYGVTLPLGRPFITACIGAAVGGAFQATM
KTASSSIGVSGLPLIPLIVDNKFLFYFIGLLITYTAGFLVTYFFGFKESMADNLK

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788769, Length=484, Percent_Identity=33.4710743801653, Blast_Score=243, Evalue=2e-65,
Organism=Escherichia coli, GI1790159, Length=426, Percent_Identity=28.4037558685446, Blast_Score=177, Evalue=1e-45,
Organism=Escherichia coli, GI2367362, Length=431, Percent_Identity=24.5939675174014, Blast_Score=145, Evalue=4e-36,
Organism=Escherichia coli, GI48994906, Length=483, Percent_Identity=26.2939958592132, Blast_Score=135, Evalue=6e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018113
- InterPro:   IPR001996
- InterPro:   IPR003352
- InterPro:   IPR013013 [H]

Pfam domain/function: PF00367 PTS_EIIB; PF02378 PTS_EIIC [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 48356; Mature: 48356

Theoretical pI: Translated: 8.18; Mature: 8.18

Prosite motif: PS01035 PTS_EIIB_TYPE_1_CYS ; PS51098 PTS_EIIB_TYPE_1 ; PS51103 PTS_EIIC_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKEERIAREILEHIGGKDNIRSFTHCMTRVRIDIKEDEKVDKSALREIEGVMGVIEDDV
CCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCC
LQIVVGPGTVNKVAAYITDITGIKIGDSSESFGEDVAKETKEGIKQKNNTPMKNLLRKIG
EEEEECCCCHHHHHHHHHHHCCEEECCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
SIFIPLIPGIVGSGIINGAAGFLKNTGVDPTLTWMQLLTVLGGSIFGYLAIVVGWNTAKE
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
FGGTPVLGAIAGIITMNPALADISLFGNNLTPGYGGLIGAMFAAWLMVFFEKQFRKMIPS
CCCCCHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
VVDIIFTPTLTILTVGMLTLVVVQPIAGILTEGITVGINWVLDVGGAFAGAVLAGFFLPL
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHEEHHHHHHCCHHHHHHHHHHHHHHH
VMFGLHHGLTPIHMELINAYDATALLPILAMAGASQVGAAIAIYVKTKNQRLKNVIKGGL
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHCCCEEEEEEECCHHHHHHHHHCCC
PTGFLGIGEPLIYGVTLPLGRPFITACIGAAVGGAFQATMKTASSSIGVSGLPLIPLIVD
CCCCHHCCCHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEHEEC
NKFLFYFIGLLITYTAGFLVTYFFGFKESMADNLK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKKEERIAREILEHIGGKDNIRSFTHCMTRVRIDIKEDEKVDKSALREIEGVMGVIEDDV
CCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCC
LQIVVGPGTVNKVAAYITDITGIKIGDSSESFGEDVAKETKEGIKQKNNTPMKNLLRKIG
EEEEECCCCHHHHHHHHHHHCCEEECCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
SIFIPLIPGIVGSGIINGAAGFLKNTGVDPTLTWMQLLTVLGGSIFGYLAIVVGWNTAKE
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
FGGTPVLGAIAGIITMNPALADISLFGNNLTPGYGGLIGAMFAAWLMVFFEKQFRKMIPS
CCCCCHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
VVDIIFTPTLTILTVGMLTLVVVQPIAGILTEGITVGINWVLDVGGAFAGAVLAGFFLPL
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHEEHHHHHHCCHHHHHHHHHHHHHHH
VMFGLHHGLTPIHMELINAYDATALLPILAMAGASQVGAAIAIYVKTKNQRLKNVIKGGL
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHCCCEEEEEEECCHHHHHHHHHCCC
PTGFLGIGEPLIYGVTLPLGRPFITACIGAAVGGAFQATMKTASSSIGVSGLPLIPLIVD
CCCCHHCCCHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEHEEC
NKFLFYFIGLLITYTAGFLVTYFFGFKESMADNLK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]