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The map label for this gene is hisZ

Identifier: 23098008

GI number: 23098008

Start: 584604

End: 585821

Strand: Reverse

Name: hisZ

Synonym: OB0553

Alternate gene names: 23098008

Gene position: 585821-584604 (Counterclockwise)

Preceding gene: 23098012

Following gene: 23098007

Centisome position: 16.14

GC content: 33.09

Gene sequence:

>1218_bases
ATGATAAATTACAATTTCCGTAATCGAACATCCTTTCCTGAAGATTTTCTAAAAAAAGCGAATGTAATAAAACAAATACA
AAATCGTTTTTATACGTATGGATATGACCAAATCGAAACCCCTCTATTTGAAGATTATGATATGTATAGCAATGTACAAG
GAACCGTTCAACAAGATGATATGGTAAAAGTAATTCATTCGTCAGGTAGAGTACTTGTATTAAGACCAGATGTGACCATA
CCTATAACACGTCAATATGTTGAAACAGATATGACTAGTCATTATCAACGATTTTCCTATTGTTTGGATATATTTCGGTT
TAACGAAACTCAACAAGCGTATCGTACACAAGCAGGGGTTGAATTCTTTGGAGATGAGAGTCCAGAAGCCGATGCTGAAG
TGATCGCTCTTGCAATTGATAGTCTTAAAGATTTGAAGATTGCTCCTTTTAAAATAGAAATTGGCCATTCTGGTATCTAT
AAAGAATTGCTTGAACAGGCAAATCTAACGGATCAAGAGCAACAAACATTACATGCGTTAATTCAGTCCAAAAATATATC
AGAAACATCTAGCTTTCTTGATCACTTATCCATCGATCAAGACTTAAAACAAAAAATAGAGCTAATTCCCATGTTGTACG
GTGATCCTTCGACTGTGATTAAAAGGGCTCAAGCAATAGTAACCAATGAAACAATGCAACAAGTTGTAGATACATTATTC
AATGTCTATTCACTATTAAAAGACGATCAGATAGAAGAATATATATCGTTTAATCTTGGATTAATTAATAATATGAATTA
TTACTCTGGAATTATTTTTCAAGGTTTCACAGAACAAATTGGTCAACCTATTTTAATGGGAGGTCGTTATGATCATCTCA
GTTCACAGTTTGAAAACGAAATACCAGCAGTAGGGTTTGCTTTTGAAATTGATAAGTTATTAACTTTGATTACTCCAGGC
GATCAACCATTAATTACACAGGCAGACTTCCTTATCGAGTATCAACCTAATTGCCGACAAGATGCATTATTACTAGCAAG
AAGGCTCCGTCAAGCAAATAAAAAAGTCATTATACAACCAACAGAATCATCGTCCAAAATAAAAGCATCAAACATCATAC
TAGTAGATTCAAATAGTTACCAACTAAAGAGTTCAGATATACAAACAACATTTTCATCCATTGATCAAACATTACAACAT
GTATTAAAAAAGAGGTGA

Upstream 100 bases:

>100_bases
GACATTTTTTCTGATTATTTATATAATACTATATAACTTTACTTAGATAAAGTGATAAAGCGGTAAAATACTAAAGAAAT
TATAGCAAAAGGAGGATATG

Downstream 100 bases:

>100_bases
ACATAATGCAACCTGTTACCATTGCTCTAGCGAAAGGAAGACCAGCGAAACAAACCATTAAGTTACTCCAAGCTGCAGGG
TTAACATTTGAAGAACTAGA

Product: histidine-tRNA ligase

Products: AMP; diphosphate +L-histidyl-tRNAHis [C]

Alternate protein names: NA

Number of amino acids: Translated: 405; Mature: 405

Protein sequence:

>405_residues
MINYNFRNRTSFPEDFLKKANVIKQIQNRFYTYGYDQIETPLFEDYDMYSNVQGTVQQDDMVKVIHSSGRVLVLRPDVTI
PITRQYVETDMTSHYQRFSYCLDIFRFNETQQAYRTQAGVEFFGDESPEADAEVIALAIDSLKDLKIAPFKIEIGHSGIY
KELLEQANLTDQEQQTLHALIQSKNISETSSFLDHLSIDQDLKQKIELIPMLYGDPSTVIKRAQAIVTNETMQQVVDTLF
NVYSLLKDDQIEEYISFNLGLINNMNYYSGIIFQGFTEQIGQPILMGGRYDHLSSQFENEIPAVGFAFEIDKLLTLITPG
DQPLITQADFLIEYQPNCRQDALLLARRLRQANKKVIIQPTESSSKIKASNIILVDSNSYQLKSSDIQTTFSSIDQTLQH
VLKKR

Sequences:

>Translated_405_residues
MINYNFRNRTSFPEDFLKKANVIKQIQNRFYTYGYDQIETPLFEDYDMYSNVQGTVQQDDMVKVIHSSGRVLVLRPDVTI
PITRQYVETDMTSHYQRFSYCLDIFRFNETQQAYRTQAGVEFFGDESPEADAEVIALAIDSLKDLKIAPFKIEIGHSGIY
KELLEQANLTDQEQQTLHALIQSKNISETSSFLDHLSIDQDLKQKIELIPMLYGDPSTVIKRAQAIVTNETMQQVVDTLF
NVYSLLKDDQIEEYISFNLGLINNMNYYSGIIFQGFTEQIGQPILMGGRYDHLSSQFENEIPAVGFAFEIDKLLTLITPG
DQPLITQADFLIEYQPNCRQDALLLARRLRQANKKVIIQPTESSSKIKASNIILVDSNSYQLKSSDIQTTFSSIDQTLQH
VLKKR
>Mature_405_residues
MINYNFRNRTSFPEDFLKKANVIKQIQNRFYTYGYDQIETPLFEDYDMYSNVQGTVQQDDMVKVIHSSGRVLVLRPDVTI
PITRQYVETDMTSHYQRFSYCLDIFRFNETQQAYRTQAGVEFFGDESPEADAEVIALAIDSLKDLKIAPFKIEIGHSGIY
KELLEQANLTDQEQQTLHALIQSKNISETSSFLDHLSIDQDLKQKIELIPMLYGDPSTVIKRAQAIVTNETMQQVVDTLF
NVYSLLKDDQIEEYISFNLGLINNMNYYSGIIFQGFTEQIGQPILMGGRYDHLSSQFENEIPAVGFAFEIDKLLTLITPG
DQPLITQADFLIEYQPNCRQDALLLARRLRQANKKVIIQPTESSSKIKASNIILVDSNSYQLKSSDIQTTFSSIDQTLQH
VLKKR

Specific function: Required for the first step of histidine biosynthesis. May allow the feedback regulation of ATP phosphoribosyltransferase activity by histidine

COG id: COG3705

COG function: function code E; ATP phosphoribosyltransferase involved in histidine biosynthesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. HisZ subfamily

Homologues:

Organism=Homo sapiens, GI6996014, Length=347, Percent_Identity=21.0374639769452, Blast_Score=66, Evalue=5e-11,
Organism=Escherichia coli, GI1788861, Length=365, Percent_Identity=25.7534246575342, Blast_Score=86, Evalue=6e-18,
Organism=Saccharomyces cerevisiae, GI6325290, Length=351, Percent_Identity=22.5071225071225, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24643061, Length=356, Percent_Identity=22.1910112359551, Blast_Score=76, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HISZ_OCEIH (Q8CXM7)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_691474.1
- ProteinModelPortal:   Q8CXM7
- SMR:   Q8CXM7
- GeneID:   1015851
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB0553
- NMPDR:   fig|221109.1.peg.556
- OMA:   YREHVKS
- BioCyc:   OIHE221109:OB0553-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00125
- InterPro:   IPR002314
- InterPro:   IPR004517
- InterPro:   IPR004516
- PANTHER:   PTHR11476
- PIRSF:   PIRSF001549
- TIGRFAMs:   TIGR00443

Pfam domain/function: PF00587 tRNA-synt_2b

EC number: 6.1.1.21 [C]

Molecular weight: Translated: 46519; Mature: 46519

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINYNFRNRTSFPEDFLKKANVIKQIQNRFYTYGYDQIETPLFEDYDMYSNVQGTVQQDD
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEECCCHHHCCCCCCCCHHHCCCCCCCCHHH
MVKVIHSSGRVLVLRPDVTIPITRQYVETDMTSHYQRFSYCLDIFRFNETQQAYRTQAGV
HHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCC
EFFGDESPEADAEVIALAIDSLKDLKIAPFKIEIGHSGIYKELLEQANLTDQEQQTLHAL
CCCCCCCCCCCHHHHHHHHHHHCCCEECCEEEEECCCHHHHHHHHHCCCCCHHHHHHHHH
IQSKNISETSSFLDHLSIDQDLKQKIELIPMLYGDPSTVIKRAQAIVTNETMQQVVDTLF
HHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHHHHHHHHHHH
NVYSLLKDDQIEEYISFNLGLINNMNYYSGIIFQGFTEQIGQPILMGGRYDHLSSQFENE
HHHHHHCCHHHHHHHHHCCCCCCCCCHHHHHHEECHHHHCCCCEEECCCHHHHHHHHHCC
IPAVGFAFEIDKLLTLITPGDQPLITQADFLIEYQPNCRQDALLLARRLRQANKKVIIQP
CCCEEEHEEHHHHHEEECCCCCCCEEECCEEEEECCCCCHHHHHHHHHHHHCCCEEEEEE
TESSSKIKASNIILVDSNSYQLKSSDIQTTFSSIDQTLQHVLKKR
CCCCCCEEECEEEEEECCCCEEECHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MINYNFRNRTSFPEDFLKKANVIKQIQNRFYTYGYDQIETPLFEDYDMYSNVQGTVQQDD
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEECCCHHHCCCCCCCCHHHCCCCCCCCHHH
MVKVIHSSGRVLVLRPDVTIPITRQYVETDMTSHYQRFSYCLDIFRFNETQQAYRTQAGV
HHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCC
EFFGDESPEADAEVIALAIDSLKDLKIAPFKIEIGHSGIYKELLEQANLTDQEQQTLHAL
CCCCCCCCCCCHHHHHHHHHHHCCCEECCEEEEECCCHHHHHHHHHCCCCCHHHHHHHHH
IQSKNISETSSFLDHLSIDQDLKQKIELIPMLYGDPSTVIKRAQAIVTNETMQQVVDTLF
HHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHHHHHHHHHHH
NVYSLLKDDQIEEYISFNLGLINNMNYYSGIIFQGFTEQIGQPILMGGRYDHLSSQFENE
HHHHHHCCHHHHHHHHHCCCCCCCCCHHHHHHEECHHHHCCCCEEECCCHHHHHHHHHCC
IPAVGFAFEIDKLLTLITPGDQPLITQADFLIEYQPNCRQDALLLARRLRQANKKVIIQP
CCCEEEHEEHHHHHEEECCCCCCCEEECCEEEEECCCCCHHHHHHHHHHHHCCCEEEEEE
TESSSKIKASNIILVDSNSYQLKSSDIQTTFSSIDQTLQHVLKKR
CCCCCCEEECEEEEEECCCCEEECHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Mg2+ [C]

Kcat value (1/min): 8520 [C]

Specific activity: 121.8

Km value (mM): 0.06 {His}} 0.046 {His}} 0.03 {His}} 0.02 {His}} 0.017 {His}} 0.011 {His}} 0.008 {His}} 0.0014 {tRNAHis}} 0.00056 {tRNAHis}} 0.0016 {U73tRNAHisGUG}} 0.00031 {U73tRNAHisGUG}} 0.89 {ATP}} 0.74 {ATP}} 0.655 {ATP}} 0.5

Substrates: ATP; @HIST01.txt*L-histidine!; tRNAHis [C]

Specific reaction: ATP + @HIST01.txt*L-histidine! + tRNAHis = AMP + diphosphate +L-histidyl-tRNAHis [C]

General reaction: Aminoacylation; Esterification [C]

Inhibitor: 1, 2, 4-Triazole-3-alanine; D-Histidine; Diphosphate; L-Histidinol; Mg2+; N-Acetyl histidine; NEM; p-Chloromercuribenzoate [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12235376