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The map label for this gene is spoT [H]

Identifier: 23097991

GI number: 23097991

Start: 569474

End: 570022

Strand: Reverse

Name: spoT [H]

Synonym: OB0536

Alternate gene names: 23097991

Gene position: 570022-569474 (Counterclockwise)

Preceding gene: 23098000

Following gene: 23097989

Centisome position: 15.7

GC content: 35.7

Gene sequence:

>549_bases
ATGTTAAAAGAGAAGGCAAAAACCTTTGCAACAAAGGCACATCAAGGGCAAACCAGAAAAAATTCGGACGAAGCATATAT
CACACATCCAATTCGTGTTGCTCAAAGGCTTGAGCTAGAAGGCTGTTCGGATGCACTGGTATGTGCAGCTTATCTTCATG
ACGTTGTTGAAGATACTCCTGTTACAATCGATGACATCCAATATGAGTTCGGAAAGGAAATTGCTCGACTTGTTGCTGCT
CATACAGAAGATAAATCCAAATCTTGGAAAGAACGCAAACAACATACAATAAAAAGCATCCAAAACTCCCCTAAGGAAGT
AAAATATTTAATTATCGCCGATAAATTAGACAACTTAATTGAACTTGAAAAAGAATACGCTTTATTGGGAGATAGTATCT
GGGCAAATTTTAATGCAGGTTACGATCAACAAAAGTGGTATAATCAATCGATCGTAAAATATATGTACTGCGGTTTAAAC
CAATCAGAAATACCAGTTTATTTTACTGAATATGCAAAAGCAGTAGAACGTGTTTTTTCTCAAAAATAA

Upstream 100 bases:

>100_bases
AATAAATTAATTTCACTGAATAAAATGATAATTATGATATAACAGTAAGTTGTTTATTTCATATGCTATAAATACTAAAC
ATCTATATTCGGAGGATACT

Downstream 100 bases:

>100_bases
CACCTTCGAATGATTGCATCGTTTGAATGTATCAGTTCGAAGGTGTTATTTAATGATTCAAGTAATATCAGGACATGAAT
TATTTATTATTCGTATTACA

Product: hypothetical protein

Products: NA

Alternate protein names: Penta-phosphate guanosine-3'-pyrophosphohydrolase; (ppGpp)ase [H]

Number of amino acids: Translated: 182; Mature: 182

Protein sequence:

>182_residues
MLKEKAKTFATKAHQGQTRKNSDEAYITHPIRVAQRLELEGCSDALVCAAYLHDVVEDTPVTIDDIQYEFGKEIARLVAA
HTEDKSKSWKERKQHTIKSIQNSPKEVKYLIIADKLDNLIELEKEYALLGDSIWANFNAGYDQQKWYNQSIVKYMYCGLN
QSEIPVYFTEYAKAVERVFSQK

Sequences:

>Translated_182_residues
MLKEKAKTFATKAHQGQTRKNSDEAYITHPIRVAQRLELEGCSDALVCAAYLHDVVEDTPVTIDDIQYEFGKEIARLVAA
HTEDKSKSWKERKQHTIKSIQNSPKEVKYLIIADKLDNLIELEKEYALLGDSIWANFNAGYDQQKWYNQSIVKYMYCGLN
QSEIPVYFTEYAKAVERVFSQK
>Mature_182_residues
MLKEKAKTFATKAHQGQTRKNSDEAYITHPIRVAQRLELEGCSDALVCAAYLHDVVEDTPVTIDDIQYEFGKEIARLVAA
HTEDKSKSWKERKQHTIKSIQNSPKEVKYLIIADKLDNLIELEKEYALLGDSIWANFNAGYDQQKWYNQSIVKYMYCGLN
QSEIPVYFTEYAKAVERVFSQK

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the degradation of ppGpp into G

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Homo sapiens, GI38348360, Length=123, Percent_Identity=40.650406504065, Blast_Score=80, Evalue=1e-15,
Organism=Escherichia coli, GI1790082, Length=124, Percent_Identity=35.4838709677419, Blast_Score=65, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI25143118, Length=122, Percent_Identity=40.9836065573771, Blast_Score=79, Evalue=9e-16,
Organism=Drosophila melanogaster, GI24650996, Length=118, Percent_Identity=38.9830508474576, Blast_Score=79, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811 [H]

Pfam domain/function: PF04607 RelA_SpoT [H]

EC number: =3.1.7.2 [H]

Molecular weight: Translated: 21030; Mature: 21030

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKEKAKTFATKAHQGQTRKNSDEAYITHPIRVAQRLELEGCSDALVCAAYLHDVVEDTP
CCCHHHHHHHHHHHCCCCCCCCCCEEEEHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCC
VTIDDIQYEFGKEIARLVAAHTEDKSKSWKERKQHTIKSIQNSPKEVKYLIIADKLDNLI
CCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHH
ELEKEYALLGDSIWANFNAGYDQQKWYNQSIVKYMYCGLNQSEIPVYFTEYAKAVERVFS
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHC
QK
CC
>Mature Secondary Structure
MLKEKAKTFATKAHQGQTRKNSDEAYITHPIRVAQRLELEGCSDALVCAAYLHDVVEDTP
CCCHHHHHHHHHHHCCCCCCCCCCEEEEHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCC
VTIDDIQYEFGKEIARLVAAHTEDKSKSWKERKQHTIKSIQNSPKEVKYLIIADKLDNLI
CCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHH
ELEKEYALLGDSIWANFNAGYDQQKWYNQSIVKYMYCGLNQSEIPVYFTEYAKAVERVFS
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHC
QK
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8948633 [H]