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The map label for this gene is acsA1 [H]

Identifier: 23097959

GI number: 23097959

Start: 538907

End: 540892

Strand: Reverse

Name: acsA1 [H]

Synonym: OB0504

Alternate gene names: 23097959

Gene position: 540892-538907 (Counterclockwise)

Preceding gene: 23097960

Following gene: 23097947

Centisome position: 14.9

GC content: 37.21

Gene sequence:

>1986_bases
GTGAAAGTAGAAGAAGGTACATTATTATGGGAACCAAGCGACCAACAAATTAAACAATCAAATATGTATGCATATCAACA
ATGGCTAGCAAACAATAAACAACTTCATTTGAACGATTATGCTTCTTTGTGGAAATGGTCAGTAGACGATATTAGTTCTT
TTTGGGAAACGATATGGGAATACTTTAATGTAATTTCTCACCACCCTTATCAACATGTAATATCAAGTACATCAATGCCT
GGTTCTAGATGGTTTAAAGAAGCGAAAGTCAATTATGCAGAACATGTATTTAAGCATAAAGATAATTCCAACCCTGCCAT
CATACATGCTTCAGAAACAAGAGGAAAGCAAGAAATTTCCTGGCAACAACTTTATCAAGATACGACTGCTCTTCAACTTA
CACTTAAGAATATCGGTGTAACAAAAGGAGACCGAGTAGTTAGTTATGCTCCTAATATTTATGAAACTGTGGTCGCTTTT
CTAGCTACTTCTAGTCTCGGAGCAATTTGGTCCAGTGCATCTCCTGACTTTGGTAAACAAAGTGTTATCGAGCGATTCCA
GCAAATTGAACCTAAAGTTATGATTACCATAGATGGCTATCAATATGGTGGTAAAAGCTTTGATCGAATGGCGATTATTG
AAGAGATTCAATCAACAATACCTTCTTTAGAAGCTACGATTGCGATCCCTTACTTGCATGCCGATACAGATTTTTCTAAT
TTAAAACAACCAATAACATGGAAAGATGCAGTGCACACTAGTAAACCCTTAGAACTAACCTTTACACCTGTCGGCTTTAA
TGATCCACTTTGGGTACTCTATTCATCTGGTACAACAGGAAAGCCGAAAGCAATCGTTCATAGTCAGGGTGGTATGTTAC
TCGAGCATCTAAAAGCCACTCACTTACATCTCGATTTAGATCAGAATTCTCGATTTTTCTGGTTTACTACAACCGGATGG
ATGATGTGGAATTTTCTTGTTGGCGGGCTCCTTAATGGTAGCTGTATTATTTTATACGATGGAAGTCCATCCTACCCAGA
TGAACGTTTCTTATGGAAGTTTGCTGAGGATACAAAAATGACTGTATTTGGAACCAGTGCCAGTTTCATAACTTCTTCGA
TGAAGAAAGATATCCACCCAGAAGAAGAATTTGATCTTTCTGCTTTAAAAAACATAAGTTCGACTGGATCTCCGTTACCT
CCTGAAGGGTTTCAATGGTGTTATACTCATGTAAAAAAAGATATTTGGGTATCTTCTGTTAGTGGCGGAACCGATGTTTG
TACGGCCTTTTTACTTGGCTCTCCAACTCTACCAGTATATGCGGGTGAATTGCAGTGTAGTGGTCTAGGAGCAAAAGTGG
AAAGTTTTGATTCAAATGGAAATTCATTAGTTAATGAAATTGGCGAGTTAGTACTAACAAAACCATTTCCATGTATGCCG
ATCTTCTTCTGGAATGACGATGATGGTATGCGAATGTATCATAGCTATTTTGACACTTATCGCGGGGTCTGGTGTCACGG
TGATTATATAAAAATCACGGAAAAAGGCACTTCTATTATCTATGGTCGTTCGGACGCAACAATCAATCGCGGAGGTATTC
GTATTGGTACAAGTGAGATCTATCGAGCAGTTGATCAAATCGAAGAAGTAGAAGATAGTTTAATGATAGATTTACCTCAA
ACCAACGGAGACTCGATTACTCCTTTGTTTGTAGTAATGAAAAATAGTAAGCCTTTCACAGATGAAGTGAAAAACTCTAT
TAAAAATAACTTAAAAACGCACTGTTCACCAAGACATGTGCCTACTCATATGTTTGAAGTGAAGGAATTGCCTAAAACAT
TAAATGGCAAAAAGATTGAAGTTCCTATAAAGAAAATATTAATGGGCGAAAGTTTTGAGCAAGTAATGAATAAAGGATCG
CTTATCAACGAAAGTGCTATTGAATATTTTATTAACTTAGCCAGAGAATGGAATTCCAGCGTCTGA

Upstream 100 bases:

>100_bases
AGAAATCTTCATACATGCGGGTTAAAAGTACCCTTTACGTAAAGAAAACGTTTTCATATAATAATATATAAAGAGTTTAG
AGTTAAAGGGGGAAAAGAGT

Downstream 100 bases:

>100_bases
ATTAGAACAAAAAACTTGGAATAGTAGCCAACAAAAATCAGTTGGGAGCTATTCCTTTTTTTGGTTTATGAGTAAACAGG
GACTTACACACTTTATATGA

Product: acetoacetyl-CoA synthetase

Products: NA

Alternate protein names: Acetoacetate--CoA ligase 1; Acyl-activating enzyme 1 [H]

Number of amino acids: Translated: 661; Mature: 661

Protein sequence:

>661_residues
MKVEEGTLLWEPSDQQIKQSNMYAYQQWLANNKQLHLNDYASLWKWSVDDISSFWETIWEYFNVISHHPYQHVISSTSMP
GSRWFKEAKVNYAEHVFKHKDNSNPAIIHASETRGKQEISWQQLYQDTTALQLTLKNIGVTKGDRVVSYAPNIYETVVAF
LATSSLGAIWSSASPDFGKQSVIERFQQIEPKVMITIDGYQYGGKSFDRMAIIEEIQSTIPSLEATIAIPYLHADTDFSN
LKQPITWKDAVHTSKPLELTFTPVGFNDPLWVLYSSGTTGKPKAIVHSQGGMLLEHLKATHLHLDLDQNSRFFWFTTTGW
MMWNFLVGGLLNGSCIILYDGSPSYPDERFLWKFAEDTKMTVFGTSASFITSSMKKDIHPEEEFDLSALKNISSTGSPLP
PEGFQWCYTHVKKDIWVSSVSGGTDVCTAFLLGSPTLPVYAGELQCSGLGAKVESFDSNGNSLVNEIGELVLTKPFPCMP
IFFWNDDDGMRMYHSYFDTYRGVWCHGDYIKITEKGTSIIYGRSDATINRGGIRIGTSEIYRAVDQIEEVEDSLMIDLPQ
TNGDSITPLFVVMKNSKPFTDEVKNSIKNNLKTHCSPRHVPTHMFEVKELPKTLNGKKIEVPIKKILMGESFEQVMNKGS
LINESAIEYFINLAREWNSSV

Sequences:

>Translated_661_residues
MKVEEGTLLWEPSDQQIKQSNMYAYQQWLANNKQLHLNDYASLWKWSVDDISSFWETIWEYFNVISHHPYQHVISSTSMP
GSRWFKEAKVNYAEHVFKHKDNSNPAIIHASETRGKQEISWQQLYQDTTALQLTLKNIGVTKGDRVVSYAPNIYETVVAF
LATSSLGAIWSSASPDFGKQSVIERFQQIEPKVMITIDGYQYGGKSFDRMAIIEEIQSTIPSLEATIAIPYLHADTDFSN
LKQPITWKDAVHTSKPLELTFTPVGFNDPLWVLYSSGTTGKPKAIVHSQGGMLLEHLKATHLHLDLDQNSRFFWFTTTGW
MMWNFLVGGLLNGSCIILYDGSPSYPDERFLWKFAEDTKMTVFGTSASFITSSMKKDIHPEEEFDLSALKNISSTGSPLP
PEGFQWCYTHVKKDIWVSSVSGGTDVCTAFLLGSPTLPVYAGELQCSGLGAKVESFDSNGNSLVNEIGELVLTKPFPCMP
IFFWNDDDGMRMYHSYFDTYRGVWCHGDYIKITEKGTSIIYGRSDATINRGGIRIGTSEIYRAVDQIEEVEDSLMIDLPQ
TNGDSITPLFVVMKNSKPFTDEVKNSIKNNLKTHCSPRHVPTHMFEVKELPKTLNGKKIEVPIKKILMGESFEQVMNKGS
LINESAIEYFINLAREWNSSV
>Mature_661_residues
MKVEEGTLLWEPSDQQIKQSNMYAYQQWLANNKQLHLNDYASLWKWSVDDISSFWETIWEYFNVISHHPYQHVISSTSMP
GSRWFKEAKVNYAEHVFKHKDNSNPAIIHASETRGKQEISWQQLYQDTTALQLTLKNIGVTKGDRVVSYAPNIYETVVAF
LATSSLGAIWSSASPDFGKQSVIERFQQIEPKVMITIDGYQYGGKSFDRMAIIEEIQSTIPSLEATIAIPYLHADTDFSN
LKQPITWKDAVHTSKPLELTFTPVGFNDPLWVLYSSGTTGKPKAIVHSQGGMLLEHLKATHLHLDLDQNSRFFWFTTTGW
MMWNFLVGGLLNGSCIILYDGSPSYPDERFLWKFAEDTKMTVFGTSASFITSSMKKDIHPEEEFDLSALKNISSTGSPLP
PEGFQWCYTHVKKDIWVSSVSGGTDVCTAFLLGSPTLPVYAGELQCSGLGAKVESFDSNGNSLVNEIGELVLTKPFPCMP
IFFWNDDDGMRMYHSYFDTYRGVWCHGDYIKITEKGTSIIYGRSDATINRGGIRIGTSEIYRAVDQIEEVEDSLMIDLPQ
TNGDSITPLFVVMKNSKPFTDEVKNSIKNNLKTHCSPRHVPTHMFEVKELPKTLNGKKIEVPIKKILMGESFEQVMNKGS
LINESAIEYFINLAREWNSSV

Specific function: Involved in poly-3-hydroxybutyrate degradation. Activates acetoacetate to acetoacetyl-CoA [H]

COG id: COG0365

COG function: function code I; Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI31982927, Length=646, Percent_Identity=37.7708978328173, Blast_Score=479, Evalue=1e-135,
Organism=Homo sapiens, GI28416953, Length=567, Percent_Identity=27.8659611992945, Blast_Score=192, Evalue=8e-49,
Organism=Homo sapiens, GI8923896, Length=646, Percent_Identity=23.8390092879257, Blast_Score=162, Evalue=1e-39,
Organism=Homo sapiens, GI253970489, Length=651, Percent_Identity=23.963133640553, Blast_Score=157, Evalue=5e-38,
Organism=Homo sapiens, GI13375727, Length=632, Percent_Identity=23.7341772151899, Blast_Score=139, Evalue=1e-32,
Organism=Homo sapiens, GI42544132, Length=541, Percent_Identity=21.0720887245841, Blast_Score=87, Evalue=5e-17,
Organism=Homo sapiens, GI38505220, Length=526, Percent_Identity=21.6730038022814, Blast_Score=84, Evalue=4e-16,
Organism=Homo sapiens, GI157311624, Length=567, Percent_Identity=22.5749559082892, Blast_Score=76, Evalue=1e-13,
Organism=Homo sapiens, GI157311622, Length=567, Percent_Identity=22.5749559082892, Blast_Score=76, Evalue=1e-13,
Organism=Escherichia coli, GI1790505, Length=626, Percent_Identity=23.482428115016, Blast_Score=193, Evalue=3e-50,
Organism=Escherichia coli, GI1786529, Length=631, Percent_Identity=23.7717908082409, Blast_Score=163, Evalue=4e-41,
Organism=Caenorhabditis elegans, GI17569507, Length=655, Percent_Identity=35.1145038167939, Blast_Score=410, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI71983001, Length=656, Percent_Identity=24.6951219512195, Blast_Score=162, Evalue=6e-40,
Organism=Caenorhabditis elegans, GI71982997, Length=656, Percent_Identity=24.6951219512195, Blast_Score=161, Evalue=8e-40,
Organism=Saccharomyces cerevisiae, GI6319264, Length=648, Percent_Identity=26.3888888888889, Blast_Score=187, Evalue=3e-48,
Organism=Saccharomyces cerevisiae, GI6323182, Length=686, Percent_Identity=24.198250728863, Blast_Score=184, Evalue=3e-47,
Organism=Drosophila melanogaster, GI24667955, Length=552, Percent_Identity=26.4492753623188, Blast_Score=187, Evalue=2e-47,
Organism=Drosophila melanogaster, GI62472339, Length=553, Percent_Identity=26.2206148282098, Blast_Score=186, Evalue=4e-47,
Organism=Drosophila melanogaster, GI21355225, Length=646, Percent_Identity=24.4582043343653, Blast_Score=167, Evalue=2e-41,
Organism=Drosophila melanogaster, GI24667959, Length=521, Percent_Identity=25.9117082533589, Blast_Score=165, Evalue=1e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005914
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.16 [H]

Molecular weight: Translated: 74810; Mature: 74810

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVEEGTLLWEPSDQQIKQSNMYAYQQWLANNKQLHLNDYASLWKWSVDDISSFWETIWE
CCCCCCCEEECCCHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHCCCHHHHHHHHHHHHH
YFNVISHHPYQHVISSTSMPGSRWFKEAKVNYAEHVFKHKDNSNPAIIHASETRGKQEIS
HHHHHHCCCHHHHHHCCCCCHHHHHHHHHCCHHHHHHHCCCCCCCEEEEECCCCCCHHCC
WQQLYQDTTALQLTLKNIGVTKGDRVVSYAPNIYETVVAFLATSSLGAIWSSASPDFGKQ
HHHHHCCCHHEEEEHHHCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
SVIERFQQIEPKVMITIDGYQYGGKSFDRMAIIEEIQSTIPSLEATIAIPYLHADTDFSN
HHHHHHHHCCCEEEEEECCEECCCCCHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCHHH
LKQPITWKDAVHTSKPLELTFTPVGFNDPLWVLYSSGTTGKPKAIVHSQGGMLLEHLKAT
CCCCCCHHHHHCCCCCCEEEEEECCCCCCEEEEECCCCCCCCCEEEECCCCHHHHHHHHE
HLHLDLDQNSRFFWFTTTGWMMWNFLVGGLLNGSCIILYDGSPSYPDERFLWKFAEDTKM
EEEEEECCCCEEEEEECHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHEEECCCCEE
TVFGTSASFITSSMKKDIHPEEEFDLSALKNISSTGSPLPPEGFQWCYTHVKKDIWVSSV
EEEECCHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHEEEEC
SGGTDVCTAFLLGSPTLPVYAGELQCSGLGAKVESFDSNGNSLVNEIGELVLTKPFPCMP
CCCHHHHHHHHHCCCCCCEEECCEEECCCCCCCCCCCCCCHHHHHHHHHHEECCCCCCEE
IFFWNDDDGMRMYHSYFDTYRGVWCHGDYIKITEKGTSIIYGRSDATINRGGIRIGTSEI
EEEECCCCCHHHHHHHHHHHCCEEEECCEEEEEECCCEEEEECCCCEEECCCEEECHHHH
YRAVDQIEEVEDSLMIDLPQTNGDSITPLFVVMKNSKPFTDEVKNSIKNNLKTHCSPRHV
HHHHHHHHHHCHHEEEEECCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCC
PTHMFEVKELPKTLNGKKIEVPIKKILMGESFEQVMNKGSLINESAIEYFINLAREWNSS
CHHHHHHHHHHHHCCCCEEEECHHHHHCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHCCC
V
C
>Mature Secondary Structure
MKVEEGTLLWEPSDQQIKQSNMYAYQQWLANNKQLHLNDYASLWKWSVDDISSFWETIWE
CCCCCCCEEECCCHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHCCCHHHHHHHHHHHHH
YFNVISHHPYQHVISSTSMPGSRWFKEAKVNYAEHVFKHKDNSNPAIIHASETRGKQEIS
HHHHHHCCCHHHHHHCCCCCHHHHHHHHHCCHHHHHHHCCCCCCCEEEEECCCCCCHHCC
WQQLYQDTTALQLTLKNIGVTKGDRVVSYAPNIYETVVAFLATSSLGAIWSSASPDFGKQ
HHHHHCCCHHEEEEHHHCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
SVIERFQQIEPKVMITIDGYQYGGKSFDRMAIIEEIQSTIPSLEATIAIPYLHADTDFSN
HHHHHHHHCCCEEEEEECCEECCCCCHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCHHH
LKQPITWKDAVHTSKPLELTFTPVGFNDPLWVLYSSGTTGKPKAIVHSQGGMLLEHLKAT
CCCCCCHHHHHCCCCCCEEEEEECCCCCCEEEEECCCCCCCCCEEEECCCCHHHHHHHHE
HLHLDLDQNSRFFWFTTTGWMMWNFLVGGLLNGSCIILYDGSPSYPDERFLWKFAEDTKM
EEEEEECCCCEEEEEECHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHEEECCCCEE
TVFGTSASFITSSMKKDIHPEEEFDLSALKNISSTGSPLPPEGFQWCYTHVKKDIWVSSV
EEEECCHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHEEEEC
SGGTDVCTAFLLGSPTLPVYAGELQCSGLGAKVESFDSNGNSLVNEIGELVLTKPFPCMP
CCCHHHHHHHHHCCCCCCEEECCEEECCCCCCCCCCCCCCHHHHHHHHHHEECCCCCCEE
IFFWNDDDGMRMYHSYFDTYRGVWCHGDYIKITEKGTSIIYGRSDATINRGGIRIGTSEI
EEEECCCCCHHHHHHHHHHHCCEEEECCEEEEEECCCEEEEECCCCEEECCCEEECHHHH
YRAVDQIEEVEDSLMIDLPQTNGDSITPLFVVMKNSKPFTDEVKNSIKNNLKTHCSPRHV
HHHHHHHHHHCHHEEEEECCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCC
PTHMFEVKELPKTLNGKKIEVPIKKILMGESFEQVMNKGSLINESAIEYFINLAREWNSS
CHHHHHHHHHHHHCCCCEEEECHHHHHCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHCCC
V
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10735852; 11481430 [H]