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The map label for this gene is thiD [H]

Identifier: 23097929

GI number: 23097929

Start: 502878

End: 503672

Strand: Reverse

Name: thiD [H]

Synonym: OB0474

Alternate gene names: 23097929

Gene position: 503672-502878 (Counterclockwise)

Preceding gene: 23097930

Following gene: 23097928

Centisome position: 13.87

GC content: 40.0

Gene sequence:

>795_bases
ATGAAGAATGTACCATGTGCATTAACGATTGCTGGCACAGACCCAAGTGGCGGAGCTGGCATACAAGCAGATTTAAAAAC
ATTTCAAGAGCTACAAACGTATGGCATGTCTGTCATTACTTCCGTAGTCGCTCAAAATACTACTGGTGTAACTTCGGTCC
ATCATATGCCAATCAATATGATCGAAGACCAACTTGAAGCTATCCTGGAAGATATGCCTATCCACGCTTTTAAAACGGGA
ATGATTGCCAATAAACAAATGATGGAAATCATCTCAAAAAAAATAGAGAAACTGAATGCACCTTATGTTATGGATCCGGT
TATGGTTGCTACCAGTGGTGATTCCCTTATAGATAAAGAAGCTCGTTCATTTTTAAGAGAAAGTTTACTCCCTCAAACGG
CACTAGTGACGCCGAATATTCCAGAGGCAGAATTTATTACTGGAAATTCTATTGAAACAGTAGAAGATATGAAAGATGCG
GCAAAACACATTGTTGATCATTTCCACGCAGATGCGGCATTAATAAAAGGTGGTCACATGGAAGGGGAAGCAATAGACTT
TCTATATGACGGATCTAAGATCCATACATTTTCAGCTGAAAGAATTAATACCAAAAACACCCATGGCACAGGGTGCACCT
ATTCTGCTGCTATTACTAGTTATCTTGCTAAGGGGTACTCCATTGTAGAAGCAGTTCGACACTCAAAAGACTTTGTTACT
GTTGCAATATCCAAATCATTTGAGCTCGGTAATGGTAATGGACCAACGAATCATTGGGCGACTCGAGAAAGGTAA

Upstream 100 bases:

>100_bases
TGAAAATTGCTTTCCGAAAAAGTTGTCAATTAGAATACTTATTTTGGGAAATGGCTATATCCACGGAGGAGTGGCCCTCA
ATAAAGGAGGATATTAAAAA

Downstream 100 bases:

>100_bases
TGGAGATATTAAGGAAAGGGAGATAAATATGCAGGTCGATATTATTAAACAGGTACGTGACACTAAACCACTTGTGCATC
ACCTAACTAATCAAGTAGTA

Product: phosphomethylpyrimidine kinase

Products: NA

Alternate protein names: Hydroxymethylpyrimidine kinase; HMP kinase; Hydroxymethylpyrimidine phosphate kinase; HMP-P kinase; HMP-phosphate kinase; HMPP kinase [H]

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MKNVPCALTIAGTDPSGGAGIQADLKTFQELQTYGMSVITSVVAQNTTGVTSVHHMPINMIEDQLEAILEDMPIHAFKTG
MIANKQMMEIISKKIEKLNAPYVMDPVMVATSGDSLIDKEARSFLRESLLPQTALVTPNIPEAEFITGNSIETVEDMKDA
AKHIVDHFHADAALIKGGHMEGEAIDFLYDGSKIHTFSAERINTKNTHGTGCTYSAAITSYLAKGYSIVEAVRHSKDFVT
VAISKSFELGNGNGPTNHWATRER

Sequences:

>Translated_264_residues
MKNVPCALTIAGTDPSGGAGIQADLKTFQELQTYGMSVITSVVAQNTTGVTSVHHMPINMIEDQLEAILEDMPIHAFKTG
MIANKQMMEIISKKIEKLNAPYVMDPVMVATSGDSLIDKEARSFLRESLLPQTALVTPNIPEAEFITGNSIETVEDMKDA
AKHIVDHFHADAALIKGGHMEGEAIDFLYDGSKIHTFSAERINTKNTHGTGCTYSAAITSYLAKGYSIVEAVRHSKDFVT
VAISKSFELGNGNGPTNHWATRER
>Mature_264_residues
MKNVPCALTIAGTDPSGGAGIQADLKTFQELQTYGMSVITSVVAQNTTGVTSVHHMPINMIEDQLEAILEDMPIHAFKTG
MIANKQMMEIISKKIEKLNAPYVMDPVMVATSGDSLIDKEARSFLRESLLPQTALVTPNIPEAEFITGNSIETVEDMKDA
AKHIVDHFHADAALIKGGHMEGEAIDFLYDGSKIHTFSAERINTKNTHGTGCTYSAAITSYLAKGYSIVEAVRHSKDFVT
VAISKSFELGNGNGPTNHWATRER

Specific function: Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P [H]

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiD family [H]

Homologues:

Organism=Escherichia coli, GI1788420, Length=257, Percent_Identity=41.6342412451362, Blast_Score=200, Evalue=9e-53,
Organism=Saccharomyces cerevisiae, GI6325378, Length=273, Percent_Identity=34.4322344322344, Blast_Score=151, Evalue=1e-37,
Organism=Saccharomyces cerevisiae, GI6324997, Length=274, Percent_Identity=38.3211678832117, Blast_Score=148, Evalue=1e-36,
Organism=Saccharomyces cerevisiae, GI6324517, Length=274, Percent_Identity=37.2262773722628, Blast_Score=142, Evalue=6e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013749
- InterPro:   IPR004399 [H]

Pfam domain/function: PF08543 Phos_pyr_kin [H]

EC number: =2.7.1.49; =2.7.4.7 [H]

Molecular weight: Translated: 28669; Mature: 28669

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNVPCALTIAGTDPSGGAGIQADLKTFQELQTYGMSVITSVVAQNTTGVTSVHHMPINM
CCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCHHH
IEDQLEAILEDMPIHAFKTGMIANKQMMEIISKKIEKLNAPYVMDPVMVATSGDSLIDKE
HHHHHHHHHHHCCHHHHHHCHHCCHHHHHHHHHHHHHCCCCEEECCEEEEECCCHHHHHH
ARSFLRESLLPQTALVTPNIPEAEFITGNSIETVEDMKDAAKHIVDHFHADAALIKGGHM
HHHHHHHHHCCCCEEECCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHCCCHHEEECCCC
EGEAIDFLYDGSKIHTFSAERINTKNTHGTGCTYSAAITSYLAKGYSIVEAVRHSKDFVT
CCCEEEEEECCCEEEEEEHHHCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHCCCCEEE
VAISKSFELGNGNGPTNHWATRER
EEEECCEECCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKNVPCALTIAGTDPSGGAGIQADLKTFQELQTYGMSVITSVVAQNTTGVTSVHHMPINM
CCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCHHH
IEDQLEAILEDMPIHAFKTGMIANKQMMEIISKKIEKLNAPYVMDPVMVATSGDSLIDKE
HHHHHHHHHHHCCHHHHHHCHHCCHHHHHHHHHHHHHCCCCEEECCEEEEECCCHHHHHH
ARSFLRESLLPQTALVTPNIPEAEFITGNSIETVEDMKDAAKHIVDHFHADAALIKGGHM
HHHHHHHHHCCCCEEECCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHCCCHHEEECCCC
EGEAIDFLYDGSKIHTFSAERINTKNTHGTGCTYSAAITSYLAKGYSIVEAVRHSKDFVT
CCCEEEEEECCCEEEEEEHHHCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHCCCCEEE
VAISKSFELGNGNGPTNHWATRER
EEEECCEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11418146 [H]