BacMap

Definition	Oceanobacillus iheyensis HTE831, complete genome.
Accession	NC_004193
Length	3,630,528

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The map label for this gene is dkgB [H]

Identifier: 23097844

GI number: 23097844

Start: 413306

End: 414148

Strand: Reverse

Name: dkgB [H]

Synonym: OB0389

Alternate gene names: 23097844

Gene position: 414148-413306 (Counterclockwise)

Preceding gene: 23097848

Following gene: 23097836

Centisome position: 11.41

GC content: 37.72

Gene sequence:

>843_bases
ATGGTAAATCAAATCCCAAACGTTACACTACATGATGGACTAACCATTCCGAAAGTAGGTTTTGGAACCTATCGTTTAAA
TGGTAACGATGGTGTACAAGCCATTAATAATGCCATTGATCATGGGTATCGTTTAATCGACACTGCCTATAACTATGAGA
ATGAAGGAACTGTTGGTGAGGCGGTTAGAAGAAGTTCCACACCTCGAGAACAGCTGCTTATTACTTCTAAACTACCAGGT
CGATATCATCACTATGATAAAGCAGTTACTACGATCCAAGAATCTTTATATCGAGCAAACCTTGATTATTATGACTTATA
TCTTATTCACTGGCCAAATCCAATCCAAGATATATATGTAGAAGCATGGCAAGCTTTAATTGAGGCCAAACGCAGGGGAT
ATATCCGTTCTATCGGAGTATGTAATTTCTTACCAGAACACTTAGAGCGACTTAAACAGGAAACTGGGGAATTACCAACG
ATAAACCAGATTGAATTACATCCTTTCTTTAACCAAGAAGACCAGCGTAAATGGCATCAAGAACATCAAATCGCTACCGA
ATCTTGGAGCCCATTGGCACGTACAAATGATGTATTAGACCATCCTGTTTTACAAAAGCTCGCTTCGAACCATAACCGAA
CGGTATCTCAAATAATTTTACGTTGGCATTATCAACTTGATGCGATTGCAATACCTAAATCTGCAACGCCTTCAAGACAG
CTCGAAAACATTTCTATTTTTGATTTTGAATTATCTGATGAAGAAATGTCTATTATTAATCAGCTATCAAAAGTGGATGG
TCGTATGAAAGATCAAGACCCAGCAGTTTATGAAGAGTTTTAA

Upstream 100 bases:

>100_bases
GCTTCACCCTTTTATAATCAAATTCACTTTTATGCAAATGGTTTGAATTTTTAATAATGCAAGCGTAATCTATAGTTTAT
ATATGGAAGGAGAGGATTAC

Downstream 100 bases:

>100_bases
GATATAACCATTTTTGGTTAAAATGCCTTTTATCTAGAAAGGCATGAATTCGTAAATTAGACTATTTCTCTTACATCATA
GGCGTAGTCACAAAACCCCC

Product: 2,5-diketo-D-gluconate reductase

Products: NA

Alternate protein names: 2,5-DKG reductase B; 2,5-DKGR B; 25DKGR-B; AKR5D [H]

Number of amino acids: Translated: 280; Mature: 280

Protein sequence:

>280_residues
MVNQIPNVTLHDGLTIPKVGFGTYRLNGNDGVQAINNAIDHGYRLIDTAYNYENEGTVGEAVRRSSTPREQLLITSKLPG
RYHHYDKAVTTIQESLYRANLDYYDLYLIHWPNPIQDIYVEAWQALIEAKRRGYIRSIGVCNFLPEHLERLKQETGELPT
INQIELHPFFNQEDQRKWHQEHQIATESWSPLARTNDVLDHPVLQKLASNHNRTVSQIILRWHYQLDAIAIPKSATPSRQ
LENISIFDFELSDEEMSIINQLSKVDGRMKDQDPAVYEEF

Sequences:

>Translated_280_residues
MVNQIPNVTLHDGLTIPKVGFGTYRLNGNDGVQAINNAIDHGYRLIDTAYNYENEGTVGEAVRRSSTPREQLLITSKLPG
RYHHYDKAVTTIQESLYRANLDYYDLYLIHWPNPIQDIYVEAWQALIEAKRRGYIRSIGVCNFLPEHLERLKQETGELPT
INQIELHPFFNQEDQRKWHQEHQIATESWSPLARTNDVLDHPVLQKLASNHNRTVSQIILRWHYQLDAIAIPKSATPSRQ
LENISIFDFELSDEEMSIINQLSKVDGRMKDQDPAVYEEF
>Mature_280_residues
MVNQIPNVTLHDGLTIPKVGFGTYRLNGNDGVQAINNAIDHGYRLIDTAYNYENEGTVGEAVRRSSTPREQLLITSKLPG
RYHHYDKAVTTIQESLYRANLDYYDLYLIHWPNPIQDIYVEAWQALIEAKRRGYIRSIGVCNFLPEHLERLKQETGELPT
INQIELHPFFNQEDQRKWHQEHQIATESWSPLARTNDVLDHPVLQKLASNHNRTVSQIILRWHYQLDAIAIPKSATPSRQ
LENISIFDFELSDEEMSIINQLSKVDGRMKDQDPAVYEEF

Specific function: Catalyzes the reduction of 2,5-diketo-D-gluconic acid (25DKG) to 2-keto-L-gulonic acid (2KLG). 25DKGR-B has higher catalytic efficiency than 25DKGR-A [H]

COG id: COG0656

COG function: function code R; Aldo/keto reductases, related to diketogulonate reductase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldo/keto reductase family [H]

Homologues:

Organism=Homo sapiens, GI223468663, Length=285, Percent_Identity=34.3859649122807, Blast_Score=170, Evalue=2e-42,
Organism=Homo sapiens, GI24497577, Length=308, Percent_Identity=32.7922077922078, Blast_Score=167, Evalue=1e-41,
Organism=Homo sapiens, GI5174391, Length=308, Percent_Identity=32.7922077922078, Blast_Score=167, Evalue=1e-41,
Organism=Homo sapiens, GI93277124, Length=295, Percent_Identity=32.5423728813559, Blast_Score=158, Evalue=7e-39,
Organism=Homo sapiens, GI5174695, Length=295, Percent_Identity=33.8983050847458, Blast_Score=152, Evalue=3e-37,
Organism=Homo sapiens, GI291291012, Length=260, Percent_Identity=34.6153846153846, Blast_Score=152, Evalue=4e-37,
Organism=Homo sapiens, GI4502049, Length=283, Percent_Identity=34.2756183745583, Blast_Score=147, Evalue=7e-36,
Organism=Homo sapiens, GI24497583, Length=294, Percent_Identity=32.6530612244898, Blast_Score=146, Evalue=2e-35,
Organism=Homo sapiens, GI5453543, Length=294, Percent_Identity=32.6530612244898, Blast_Score=145, Evalue=3e-35,
Organism=Homo sapiens, GI310109922, Length=276, Percent_Identity=34.0579710144928, Blast_Score=145, Evalue=4e-35,
Organism=Homo sapiens, GI310109920, Length=294, Percent_Identity=32.6530612244898, Blast_Score=145, Evalue=5e-35,
Organism=Homo sapiens, GI45446745, Length=294, Percent_Identity=32.312925170068, Blast_Score=144, Evalue=9e-35,
Organism=Homo sapiens, GI4503285, Length=294, Percent_Identity=32.312925170068, Blast_Score=144, Evalue=9e-35,
Organism=Homo sapiens, GI24497585, Length=295, Percent_Identity=31.5254237288136, Blast_Score=139, Evalue=3e-33,
Organism=Homo sapiens, GI300116273, Length=277, Percent_Identity=32.129963898917, Blast_Score=132, Evalue=3e-31,
Organism=Homo sapiens, GI300116271, Length=278, Percent_Identity=32.0143884892086, Blast_Score=120, Evalue=9e-28,
Organism=Homo sapiens, GI310109924, Length=149, Percent_Identity=32.2147651006711, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI310109926, Length=132, Percent_Identity=37.1212121212121, Blast_Score=78, Evalue=8e-15,
Organism=Homo sapiens, GI207028673, Length=125, Percent_Identity=37.6, Blast_Score=77, Evalue=2e-14,
Organism=Escherichia coli, GI87082198, Length=268, Percent_Identity=44.4029850746269, Blast_Score=234, Evalue=4e-63,
Organism=Escherichia coli, GI1786400, Length=252, Percent_Identity=36.9047619047619, Blast_Score=157, Evalue=8e-40,
Organism=Escherichia coli, GI1788081, Length=269, Percent_Identity=30.4832713754647, Blast_Score=109, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17537077, Length=305, Percent_Identity=34.7540983606557, Blast_Score=163, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI17537075, Length=302, Percent_Identity=34.7682119205298, Blast_Score=162, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI17561298, Length=270, Percent_Identity=35.1851851851852, Blast_Score=162, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI71998625, Length=274, Percent_Identity=34.3065693430657, Blast_Score=155, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17564128, Length=316, Percent_Identity=31.0126582278481, Blast_Score=148, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI17538386, Length=270, Percent_Identity=34.4444444444444, Blast_Score=146, Evalue=9e-36,
Organism=Caenorhabditis elegans, GI17537079, Length=299, Percent_Identity=30.7692307692308, Blast_Score=142, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17552492, Length=257, Percent_Identity=31.1284046692607, Blast_Score=139, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17561300, Length=273, Percent_Identity=31.5018315018315, Blast_Score=134, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI17566692, Length=291, Percent_Identity=29.553264604811, Blast_Score=134, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI17550248, Length=309, Percent_Identity=29.126213592233, Blast_Score=125, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17562292, Length=274, Percent_Identity=29.1970802919708, Blast_Score=106, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6324694, Length=292, Percent_Identity=32.5342465753425, Blast_Score=163, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6320576, Length=300, Percent_Identity=31.6666666666667, Blast_Score=156, Evalue=4e-39,
Organism=Saccharomyces cerevisiae, GI6321896, Length=314, Percent_Identity=31.2101910828025, Blast_Score=137, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6322556, Length=260, Percent_Identity=30, Blast_Score=135, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6319625, Length=294, Percent_Identity=32.312925170068, Blast_Score=126, Evalue=4e-30,
Organism=Saccharomyces cerevisiae, GI6320079, Length=280, Percent_Identity=28.5714285714286, Blast_Score=106, Evalue=5e-24,
Organism=Drosophila melanogaster, GI24663317, Length=285, Percent_Identity=32.9824561403509, Blast_Score=163, Evalue=1e-40,
Organism=Drosophila melanogaster, GI24657054, Length=281, Percent_Identity=34.8754448398576, Blast_Score=162, Evalue=2e-40,
Organism=Drosophila melanogaster, GI21356425, Length=290, Percent_Identity=34.1379310344828, Blast_Score=159, Evalue=1e-39,
Organism=Drosophila melanogaster, GI20129731, Length=286, Percent_Identity=32.5174825174825, Blast_Score=157, Evalue=6e-39,
Organism=Drosophila melanogaster, GI24644950, Length=309, Percent_Identity=31.3915857605178, Blast_Score=151, Evalue=5e-37,
Organism=Drosophila melanogaster, GI24662789, Length=304, Percent_Identity=31.25, Blast_Score=145, Evalue=2e-35,
Organism=Drosophila melanogaster, GI221378297, Length=329, Percent_Identity=29.7872340425532, Blast_Score=144, Evalue=6e-35,
Organism=Drosophila melanogaster, GI45553081, Length=280, Percent_Identity=32.1428571428571, Blast_Score=139, Evalue=2e-33,
Organism=Drosophila melanogaster, GI24662781, Length=281, Percent_Identity=33.0960854092527, Blast_Score=134, Evalue=7e-32,
Organism=Drosophila melanogaster, GI24662785, Length=280, Percent_Identity=33.5714285714286, Blast_Score=133, Evalue=1e-31,
Organism=Drosophila melanogaster, GI281366140, Length=244, Percent_Identity=31.5573770491803, Blast_Score=115, Evalue=3e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001395
- InterPro:   IPR018170
- InterPro:   IPR020471
- InterPro:   IPR023210 [H]

Pfam domain/function: PF00248 Aldo_ket_red [H]

EC number: =1.1.1.274 [H]

Molecular weight: Translated: 32479; Mature: 32479

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: PS00798 ALDOKETO_REDUCTASE_1 ; PS00062 ALDOKETO_REDUCTASE_2 ; PS00063 ALDOKETO_REDUCTASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVNQIPNVTLHDGLTIPKVGFGTYRLNGNDGVQAINNAIDHGYRLIDTAYNYENEGTVGE
CCCCCCCEEECCCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCEEHHHHHCCCCCCCHHH
AVRRSSTPREQLLITSKLPGRYHHYDKAVTTIQESLYRANLDYYDLYLIHWPNPIQDIYV
HHHHCCCCHHHEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCHHHHHHH
EAWQALIEAKRRGYIRSIGVCNFLPEHLERLKQETGELPTINQIELHPFFNQEDQRKWHQ
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHH
EHQIATESWSPLARTNDVLDHPVLQKLASNHNRTVSQIILRWHYQLDAIAIPKSATPSRQ
HHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCEEECCCCCCCCHH
LENISIFDFELSDEEMSIINQLSKVDGRMKDQDPAVYEEF
HCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCHHCCC
>Mature Secondary Structure
MVNQIPNVTLHDGLTIPKVGFGTYRLNGNDGVQAINNAIDHGYRLIDTAYNYENEGTVGE
CCCCCCCEEECCCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCEEHHHHHCCCCCCCHHH
AVRRSSTPREQLLITSKLPGRYHHYDKAVTTIQESLYRANLDYYDLYLIHWPNPIQDIYV
HHHHCCCCHHHEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCHHHHHHH
EAWQALIEAKRRGYIRSIGVCNFLPEHLERLKQETGELPTINQIELHPFFNQEDQRKWHQ
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHH
EHQIATESWSPLARTNDVLDHPVLQKLASNHNRTVSQIILRWHYQLDAIAIPKSATPSRQ
HHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCEEECCCCCCCCHH
LENISIFDFELSDEEMSIINQLSKVDGRMKDQDPAVYEEF
HCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA