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The map label for this gene is hmp

Identifier: 23097746

GI number: 23097746

Start: 324358

End: 325578

Strand: Reverse

Name: hmp

Synonym: OB0291

Alternate gene names: 23097746

Gene position: 325578-324358 (Counterclockwise)

Preceding gene: 23097748

Following gene: 23097732

Centisome position: 8.97

GC content: 35.79

Gene sequence:

>1221_bases
ATGTCAAACACAACCGTACTTTTAGACAAAAAAACGACAGAAATTATTAAAGCTACCGTACCAGTATTAAAGGAGCACGG
AGAAGCCATAACTAAGCATTTTTACAAGATACTTTTAGAAAACAATCCAGAACTAAAAAATGTCTTTAATCAAACAAATC
AACGGAAAGGAGCCCAATCGAAAGCACTAGCAAATACGGTATACGCTGCTGCAGCAAACATTGAGAAATTAGAAGAAATC
TTACCACATGTTAAACAAATTGCACATAAACATGTCAGTTTAAATATTAAACCAGAACAATATCCTATTGTTGGTAAATA
CCTGTTAATAGCGATCAAGGAAGTATTAGGAGATGCAGCTACTGACGAAATTATAGAGGCATGGGAAAAAGCATACTTTG
TTATCGCGGACATCTTTATATCCGTAGAAAAAGAGATGTACAACGAGAAAAAGAACCAGATCGGTGGATGGACCGGTTTT
CGTGATTTTAAAGTTATTAAAAAAGTAAAAGAAAGTAAGGAGATTACTTCTTTTTATCTCAAGCCGGATGATAATTTACC
AATCACCACTTTCATACCTGGGCAATATATTACGATAAAAGCACAGATAGAAAGTGAAGCTTATGTTCATTTACGACAAT
ATAGCTTATCAACGGCACCAGGGAAAGATTATTATCGTATTAGTGTAAAACGAGAAGCTTCAAATCAACCGATTGGAGTT
GTATCTAATTATCTTCACACTTCTGTGGAAGTGGGATCTGTTTTACCAATCAGTGCACCTGCTGGTGATTTTATTTTAGA
CGAACGTGACCACCGTCCTCTAGTTTTAATTAGTGGTGGAGTTGGATTAACTCCCATCATGAGTATGTTAGAATCCGTTG
TAGAACATCAGCCAAATCGGAATGTAGTATTCATTCATGCTGCCAAATCTATCGACCATCAAGCGATGAGAAAAAGAGTT
TCTGAAATAGCAAAATCTAAAGAACAAGTGAAGCAGTATGTTGTATATAGTAACCCAACGAATCGTACAGATGGAGATAA
ACAAGGCTATATCGATTATGAATGGTTAAAAGAAGTTATCCCTACAAAAGATGCTGCCTTTTACTTGTGCGGGCCGAAGC
CTTTTATGAGTGCAATAAACAATGATCTACAAAATATGAACATTGCTCAAAACGATATTCATATGGAATTATTTGGACCT
CTCGAACCTATTGCGAAGTAA

Upstream 100 bases:

>100_bases
TATTGTAACACATGGTTGCGTTAGACTTATTGTATTTAGAATGTAATCGTATAAAATAAAAGATGTATAGAAAATACATC
TTTTATTTAGGAGTGATTTC

Downstream 100 bases:

>100_bases
CAATGGAAAAGGATTGACAGTCGAGTGTCAATCCTTTTTGCCCCTTCCTGCGCAGTTTAAACACAAAAGTGTTCTATCAT
CTTGTAAAACTCCTTCTACA

Product: nitric oxide dioxygenase

Products: NA

Alternate protein names: Flavohemoglobin; Hemoglobin-like protein; Nitric oxide dioxygenase; NO oxygenase; NOD

Number of amino acids: Translated: 406; Mature: 405

Protein sequence:

>406_residues
MSNTTVLLDKKTTEIIKATVPVLKEHGEAITKHFYKILLENNPELKNVFNQTNQRKGAQSKALANTVYAAAANIEKLEEI
LPHVKQIAHKHVSLNIKPEQYPIVGKYLLIAIKEVLGDAATDEIIEAWEKAYFVIADIFISVEKEMYNEKKNQIGGWTGF
RDFKVIKKVKESKEITSFYLKPDDNLPITTFIPGQYITIKAQIESEAYVHLRQYSLSTAPGKDYYRISVKREASNQPIGV
VSNYLHTSVEVGSVLPISAPAGDFILDERDHRPLVLISGGVGLTPIMSMLESVVEHQPNRNVVFIHAAKSIDHQAMRKRV
SEIAKSKEQVKQYVVYSNPTNRTDGDKQGYIDYEWLKEVIPTKDAAFYLCGPKPFMSAINNDLQNMNIAQNDIHMELFGP
LEPIAK

Sequences:

>Translated_406_residues
MSNTTVLLDKKTTEIIKATVPVLKEHGEAITKHFYKILLENNPELKNVFNQTNQRKGAQSKALANTVYAAAANIEKLEEI
LPHVKQIAHKHVSLNIKPEQYPIVGKYLLIAIKEVLGDAATDEIIEAWEKAYFVIADIFISVEKEMYNEKKNQIGGWTGF
RDFKVIKKVKESKEITSFYLKPDDNLPITTFIPGQYITIKAQIESEAYVHLRQYSLSTAPGKDYYRISVKREASNQPIGV
VSNYLHTSVEVGSVLPISAPAGDFILDERDHRPLVLISGGVGLTPIMSMLESVVEHQPNRNVVFIHAAKSIDHQAMRKRV
SEIAKSKEQVKQYVVYSNPTNRTDGDKQGYIDYEWLKEVIPTKDAAFYLCGPKPFMSAINNDLQNMNIAQNDIHMELFGP
LEPIAK
>Mature_405_residues
SNTTVLLDKKTTEIIKATVPVLKEHGEAITKHFYKILLENNPELKNVFNQTNQRKGAQSKALANTVYAAAANIEKLEEIL
PHVKQIAHKHVSLNIKPEQYPIVGKYLLIAIKEVLGDAATDEIIEAWEKAYFVIADIFISVEKEMYNEKKNQIGGWTGFR
DFKVIKKVKESKEITSFYLKPDDNLPITTFIPGQYITIKAQIESEAYVHLRQYSLSTAPGKDYYRISVKREASNQPIGVV
SNYLHTSVEVGSVLPISAPAGDFILDERDHRPLVLISGGVGLTPIMSMLESVVEHQPNRNVVFIHAAKSIDHQAMRKRVS
EIAKSKEQVKQYVVYSNPTNRTDGDKQGYIDYEWLKEVIPTKDAAFYLCGPKPFMSAINNDLQNMNIAQNDIHMELFGPL
EPIAK

Specific function: Is involved in NO detoxification in an aerobic process, termed nitric oxide dioxygenase (NOD) reaction that utilizes O(2) and NAD(P)H to convert NO to nitrate, which protects the bacterium from various noxious nitrogen compounds. Therefore, plays a centra

COG id: COG1018

COG function: function code C; Flavodoxin reductases (ferredoxin-NADPH reductases) family 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain

Homologues:

Organism=Escherichia coli, GI1788903, Length=398, Percent_Identity=41.2060301507538, Blast_Score=318, Evalue=4e-88,
Organism=Escherichia coli, GI1787098, Length=222, Percent_Identity=24.3243243243243, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1787658, Length=253, Percent_Identity=24.901185770751, Blast_Score=69, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6321673, Length=409, Percent_Identity=45.721271393643, Blast_Score=298, Evalue=1e-81,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): HMP_OCEIH (Q8ETH0)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_691212.1
- ProteinModelPortal:   Q8ETH0
- SMR:   Q8ETH0
- GeneID:   1015481
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB0291
- NMPDR:   fig|221109.1.peg.293
- HOGENOM:   HBG623097
- OMA:   QIGHKHR
- ProtClustDB:   PRK13289
- BioCyc:   OIHE221109:OB0291-MONOMER
- BRENDA:   1.14.12.17
- HAMAP:   MF_01252
- InterPro:   IPR017927
- InterPro:   IPR001709
- InterPro:   IPR012292
- InterPro:   IPR009050
- InterPro:   IPR000971
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR001221
- InterPro:   IPR017938
- Gene3D:   G3DSA:1.10.490.10
- PRINTS:   PR00371
- PRINTS:   PR00410

Pfam domain/function: PF00970 FAD_binding_6; PF00042 Globin; PF00175 NAD_binding_1; SSF46458 Globin_like; SSF63380 Riboflavin_synthase_like_b-brl

EC number: =1.14.12.17

Molecular weight: Translated: 45811; Mature: 45680

Theoretical pI: Translated: 7.87; Mature: 7.87

Prosite motif: PS51384 FAD_FR; PS01033 GLOBIN

Important sites: ACT_SITE 101-101 ACT_SITE 143-143 BINDING 196-196

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNTTVLLDKKTTEIIKATVPVLKEHGEAITKHFYKILLENNPELKNVFNQTNQRKGAQS
CCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHH
KALANTVYAAAANIEKLEEILPHVKQIAHKHVSLNIKPEQYPIVGKYLLIAIKEVLGDAA
HHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHHHCCCH
TDEIIEAWEKAYFVIADIFISVEKEMYNEKKNQIGGWTGFRDFKVIKKVKESKEITSFYL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCEEEEE
KPDDNLPITTFIPGQYITIKAQIESEAYVHLRQYSLSTAPGKDYYRISVKREASNQPIGV
CCCCCCCEEEECCCEEEEEEEEECCCCEEEEEEHCCCCCCCCCEEEEEEEECCCCCCHHH
VSNYLHTSVEVGSVLPISAPAGDFILDERDHRPLVLISGGVGLTPIMSMLESVVEHQPNR
HHHHHHHHEECCCEEECCCCCCCCEECCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCC
NVVFIHAAKSIDHQAMRKRVSEIAKSKEQVKQYVVYSNPTNRTDGDKQGYIDYEWLKEVI
CEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCCCCHHHHHHHH
PTKDAAFYLCGPKPFMSAINNDLQNMNIAQNDIHMELFGPLEPIAK
CCCCCEEEEECCHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHCCC
>Mature Secondary Structure 
SNTTVLLDKKTTEIIKATVPVLKEHGEAITKHFYKILLENNPELKNVFNQTNQRKGAQS
CCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHH
KALANTVYAAAANIEKLEEILPHVKQIAHKHVSLNIKPEQYPIVGKYLLIAIKEVLGDAA
HHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHHHCCCH
TDEIIEAWEKAYFVIADIFISVEKEMYNEKKNQIGGWTGFRDFKVIKKVKESKEITSFYL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCEEEEE
KPDDNLPITTFIPGQYITIKAQIESEAYVHLRQYSLSTAPGKDYYRISVKREASNQPIGV
CCCCCCCEEEECCCEEEEEEEEECCCCEEEEEEHCCCCCCCCCEEEEEEEECCCCCCHHH
VSNYLHTSVEVGSVLPISAPAGDFILDERDHRPLVLISGGVGLTPIMSMLESVVEHQPNR
HHHHHHHHEECCCEEECCCCCCCCEECCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCC
NVVFIHAAKSIDHQAMRKRVSEIAKSKEQVKQYVVYSNPTNRTDGDKQGYIDYEWLKEVI
CEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCCCCHHHHHHHH
PTKDAAFYLCGPKPFMSAINNDLQNMNIAQNDIHMELFGPLEPIAK
CCCCCEEEEECCHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12235376