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The map label for this gene is cwlM [H]

Identifier: 23097675

GI number: 23097675

Start: 239037

End: 239888

Strand: Direct

Name: cwlM [H]

Synonym: OB0220

Alternate gene names: 23097675

Gene position: 239037-239888 (Clockwise)

Preceding gene: 23097674

Following gene: 23097679

Centisome position: 6.58

GC content: 38.5

Gene sequence:

>852_bases
ATGGTAAATATTTATTTAGATCCAGGACATGGTGGAAATGATCCAGGTGCAGTTGGAAATGGGCTTCAAGAAAAGAATGT
TACGTTAGCCATTGCCTTAAAGGTGAGAAATTACCTCAATACCAATTATAGCGGACACTCCATTCGAATGAGTAGAACGA
CAGATACAGCTGTTTCTTTAGCTCAACGTACAAATGATGCAAATAATTGGGGAGCGGATTATTTTGTATCGATTCATATT
AATGCTGGTGGTGGTACAGGATATGAAGATTATATCCATAGTAATCTTTCGGATAGTTCAACAATAGCGCAATATCGAAA
TGTAATGCATGAAGAAATTATGAAGCAGGTAGATTATAGAGATAGAGGGAAGAAAAAAGCGAATTTCCATGTATTGCGTG
AGTCTGCTATGCCATCTATCTTAACAGAGAACGGCTTTATTGATACGGTAGCAGATGCAAATAAATTAAAATCAGATGCT
TATTTGAATCGCATTGCATTAGGTCATGCCAATGGAATTGCTCGTGTATTTAATCTCCCGTCTGCTGGTGGACAAGATAC
CTATACGGTCCAACCTGGAGATACACTATGGAGTATTTCACAAGCATTCGATATGACTGTACAAGAACTAAAAGACCTAA
ATGGTTTAACCGATGATACAATCTATCCTGGACAAGTTCTTATCGTCAGCGGTAACGGTTTTGAGCAAGGGCCGGTGTAT
CATATTGTCCAAGCTGGAGAGACTTTATGGGGGATTGCTCAAGAATACGAAACAACTGTAGAAGCAATTAAGTCTTTAAA
TGGTTTAACCAGTGATACGATCCAACCTGGTCAACGTTTACAAGTGAAGTAA

Upstream 100 bases:

>100_bases
GATATCCTCAAGGAACACCACTTAACTGGTTAAAAAAGAAGCGATCAAAAAATGATTGCTTCTTTTTTATATAAAAATTC
ATGAAAAAGGGGAGATTTTA

Downstream 100 bases:

>100_bases
AGATGGATTTACAAAAATTAGACCCTGCGCAGTAAGCCTGCAGGGTCTATTCTATATTATTTAGTTAATGCTTTCTCTAA
TGCCTCGATATTTTTCTCCA

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: Autolysin; Cell wall hydrolase [H]

Number of amino acids: Translated: 283; Mature: 283

Protein sequence:

>283_residues
MVNIYLDPGHGGNDPGAVGNGLQEKNVTLAIALKVRNYLNTNYSGHSIRMSRTTDTAVSLAQRTNDANNWGADYFVSIHI
NAGGGTGYEDYIHSNLSDSSTIAQYRNVMHEEIMKQVDYRDRGKKKANFHVLRESAMPSILTENGFIDTVADANKLKSDA
YLNRIALGHANGIARVFNLPSAGGQDTYTVQPGDTLWSISQAFDMTVQELKDLNGLTDDTIYPGQVLIVSGNGFEQGPVY
HIVQAGETLWGIAQEYETTVEAIKSLNGLTSDTIQPGQRLQVK

Sequences:

>Translated_283_residues
MVNIYLDPGHGGNDPGAVGNGLQEKNVTLAIALKVRNYLNTNYSGHSIRMSRTTDTAVSLAQRTNDANNWGADYFVSIHI
NAGGGTGYEDYIHSNLSDSSTIAQYRNVMHEEIMKQVDYRDRGKKKANFHVLRESAMPSILTENGFIDTVADANKLKSDA
YLNRIALGHANGIARVFNLPSAGGQDTYTVQPGDTLWSISQAFDMTVQELKDLNGLTDDTIYPGQVLIVSGNGFEQGPVY
HIVQAGETLWGIAQEYETTVEAIKSLNGLTSDTIQPGQRLQVK
>Mature_283_residues
MVNIYLDPGHGGNDPGAVGNGLQEKNVTLAIALKVRNYLNTNYSGHSIRMSRTTDTAVSLAQRTNDANNWGADYFVSIHI
NAGGGTGYEDYIHSNLSDSSTIAQYRNVMHEEIMKQVDYRDRGKKKANFHVLRESAMPSILTENGFIDTVADANKLKSDA
YLNRIALGHANGIARVFNLPSAGGQDTYTVQPGDTLWSISQAFDMTVQELKDLNGLTDDTIYPGQVLIVSGNGFEQGPVY
HIVQAGETLWGIAQEYETTVEAIKSLNGLTSDTIQPGQRLQVK

Specific function: Hydrolyzes the cell wall of M.luteus more efficiently than that of B.licheniformis and B.subtilis. The C-terminal region, including the repeats, determines substrate specificity [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Secreted (Probable). Note=Accumulates in cells as inclusion bodies. May be secreted by a mechanism different from the normal export system [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788776, Length=221, Percent_Identity=28.0542986425339, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1790611, Length=224, Percent_Identity=27.2321428571429, Blast_Score=74, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002508
- InterPro:   IPR007730 [H]

Pfam domain/function: PF01520 Amidase_3; PF05036 SPOR [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 30914; Mature: 30914

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVNIYLDPGHGGNDPGAVGNGLQEKNVTLAIALKVRNYLNTNYSGHSIRMSRTTDTAVSL
CEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEEEEHHHHCCCCCCCEEEEECCHHHHHHH
AQRTNDANNWGADYFVSIHINAGGGTGYEDYIHSNLSDSSTIAQYRNVMHEEIMKQVDYR
HHHCCCCCCCCCEEEEEEEEECCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHH
DRGKKKANFHVLRESAMPSILTENGFIDTVADANKLKSDAYLNRIALGHANGIARVFNLP
HCCCCCCHHHEEHHHCCCHHHHCCCCEECHHHHHHHHHHHHHHHHHCCCCCCHHEEEECC
SAGGQDTYTVQPGDTLWSISQAFDMTVQELKDLNGLTDDTIYPGQVLIVSGNGFEQGPVY
CCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCEE
HIVQAGETLWGIAQEYETTVEAIKSLNGLTSDTIQPGQRLQVK
EEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECC
>Mature Secondary Structure
MVNIYLDPGHGGNDPGAVGNGLQEKNVTLAIALKVRNYLNTNYSGHSIRMSRTTDTAVSL
CEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEEEEHHHHCCCCCCCEEEEECCHHHHHHH
AQRTNDANNWGADYFVSIHINAGGGTGYEDYIHSNLSDSSTIAQYRNVMHEEIMKQVDYR
HHHCCCCCCCCCEEEEEEEEECCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHH
DRGKKKANFHVLRESAMPSILTENGFIDTVADANKLKSDAYLNRIALGHANGIARVFNLP
HCCCCCCHHHEEHHHCCCHHHHCCCCEECHHHHHHHHHHHHHHHHHCCCCCCHHEEEECC
SAGGQDTYTVQPGDTLWSISQAFDMTVQELKDLNGLTDDTIYPGQVLIVSGNGFEQGPVY
CCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCEE
HIVQAGETLWGIAQEYETTVEAIKSLNGLTSDTIQPGQRLQVK
EEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1495475 [H]