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The map label for this gene is tilS/hprT [H]

Identifier: 23097533

GI number: 23097533

Start: 78879

End: 79433

Strand: Direct

Name: tilS/hprT [H]

Synonym: OB0078

Alternate gene names: 23097533

Gene position: 78879-79433 (Clockwise)

Preceding gene: 23097532

Following gene: 23097534

Centisome position: 2.17

GC content: 33.87

Gene sequence:

>555_bases
ATGCTTCAAGACAACATACACGGCGATATTCAAGACATTTTAATAACAGAGGAACAAATTCAGGAGAAATGTAAAGAACT
CGGATCAATAATTTCGAAAGAATATGATGGTAAGTTTCCGCTTGCTATCGGAGTACTAAAAGGTGCTATGCCTTTTATGT
CAGATATTCTTCGATACTCAGAGATTCATTTAGAAATGGATTTTATGGACGTTTCTAGTTATGATGGCGGGACAACTTCA
ACTGGTGAAGTGAAAATCTTAAAAGATTTAAATACAAAAGTTGAAGGTCGAGATTTAATAATAGTTGAAGACATAATAGA
TAGCGGTTTAACGTTAAGCTACTTAGTAGACTTGTTTAAATACCGTAAAGCAAATTCCATAAAGATAGTGACGTTATTAG
ATAAACCATCTGGAAGAAATGCAGCGATAAAAGCAGATGTGGTAGGATTTGAAGTTCCAGATGAATTTGTAGTTGGTTAT
GGTCTAGATTACGCTGAAAAATATCGGAATTTACCATATATCGGTATACTTAAACCGGAAGTATATGGTGGATAA

Upstream 100 bases:

>100_bases
TGGTAGGGCTCAAAAAAGCGTTTGGTTCGAATCAATTTTGTCAAAGTGGAGAAAAGATAAAACTTAGCTATCATAAAGGG
AATATTTAGGAGGATGAATC

Downstream 100 bases:

>100_bases
TAACTTTGATTATATAAATCAGGGTTGGATAAAATAAATAATCTAATAAATAATTGGAAAAATTTAATCAAAAAATGGTT
ATATCATTTGTATTAAAACT

Product: hypoxanthine phosphoribosyltransferase

Products: NA

Alternate protein names: tRNA(Ile)-lysidine synthase; tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase; Hypoxanthine-guanine phosphoribosyltransferase; HGPRT; HGPRTase [H]

Number of amino acids: Translated: 184; Mature: 184

Protein sequence:

>184_residues
MLQDNIHGDIQDILITEEQIQEKCKELGSIISKEYDGKFPLAIGVLKGAMPFMSDILRYSEIHLEMDFMDVSSYDGGTTS
TGEVKILKDLNTKVEGRDLIIVEDIIDSGLTLSYLVDLFKYRKANSIKIVTLLDKPSGRNAAIKADVVGFEVPDEFVVGY
GLDYAEKYRNLPYIGILKPEVYGG

Sequences:

>Translated_184_residues
MLQDNIHGDIQDILITEEQIQEKCKELGSIISKEYDGKFPLAIGVLKGAMPFMSDILRYSEIHLEMDFMDVSSYDGGTTS
TGEVKILKDLNTKVEGRDLIIVEDIIDSGLTLSYLVDLFKYRKANSIKIVTLLDKPSGRNAAIKADVVGFEVPDEFVVGY
GLDYAEKYRNLPYIGILKPEVYGG
>Mature_184_residues
MLQDNIHGDIQDILITEEQIQEKCKELGSIISKEYDGKFPLAIGVLKGAMPFMSDILRYSEIHLEMDFMDVSSYDGGTTS
TGEVKILKDLNTKVEGRDLIIVEDIIDSGLTLSYLVDLFKYRKANSIKIVTLLDKPSGRNAAIKADVVGFEVPDEFVVGY
GLDYAEKYRNLPYIGILKPEVYGG

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0634

COG function: function code F; Hypoxanthine-guanine phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI4504483, Length=180, Percent_Identity=35.5555555555556, Blast_Score=109, Evalue=1e-24,
Organism=Homo sapiens, GI9910262, Length=183, Percent_Identity=31.6939890710383, Blast_Score=92, Evalue=2e-19,
Organism=Escherichia coli, GI87081689, Length=166, Percent_Identity=46.9879518072289, Blast_Score=162, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI17510817, Length=180, Percent_Identity=31.6666666666667, Blast_Score=89, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005904
- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR020825
- InterPro:   IPR000836
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3; PF00156 Pribosyltran; PF11734 TilS_C [H]

EC number: =2.4.2.8 [H]

Molecular weight: Translated: 20535; Mature: 20535

Theoretical pI: Translated: 4.39; Mature: 4.39

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQDNIHGDIQDILITEEQIQEKCKELGSIISKEYDGKFPLAIGVLKGAMPFMSDILRYS
CCCCCCCCCHHHHEECHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCHHHHHHHHHHH
EIHLEMDFMDVSSYDGGTTSTGEVKILKDLNTKVEGRDLIIVEDIIDSGLTLSYLVDLFK
HHEEEEEEEEECCCCCCCCCCCCEEEEECCCCCCCCCEEEEEHHHHCCCCHHHHHHHHHH
YRKANSIKIVTLLDKPSGRNAAIKADVVGFEVPDEFVVGYGLDYAEKYRNLPYIGILKPE
HCCCCCEEEEEEEECCCCCCCEEEEEEEEEECCCHHEEECCCCHHHHHCCCCEEEEECCC
VYGG
CCCC
>Mature Secondary Structure
MLQDNIHGDIQDILITEEQIQEKCKELGSIISKEYDGKFPLAIGVLKGAMPFMSDILRYS
CCCCCCCCCHHHHEECHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCHHHHHHHHHHH
EIHLEMDFMDVSSYDGGTTSTGEVKILKDLNTKVEGRDLIIVEDIIDSGLTLSYLVDLFK
HHEEEEEEEEECCCCCCCCCCCCEEEEECCCCCCCCCEEEEEHHHHCCCCHHHHHHHHHH
YRKANSIKIVTLLDKPSGRNAAIKADVVGFEVPDEFVVGYGLDYAEKYRNLPYIGILKPE
HCCCCCEEEEEEEECCCCCCCEEEEEEEEEECCCHHEEECCCCHHHHHCCCCEEEEECCC
VYGG
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]