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The map label for this gene is tilS

Identifier: 23097532

GI number: 23097532

Start: 77467

End: 78867

Strand: Direct

Name: tilS

Synonym: OB0077

Alternate gene names: 23097532

Gene position: 77467-78867 (Clockwise)

Preceding gene: 23097531

Following gene: 23097533

Centisome position: 2.13

GC content: 35.69

Gene sequence:

>1401_bases
ATGAAACAATCTATTCTTTCCTTTATTCAAAAACATCAATTACTAAAAGAAGGTGCAAAAATTATCGTTGGTGTATCTGG
TGGATCGGATTCAATGGCGTTACTGCATTTTCTTAAAAATTTGCAAGAGCAATGGGAAATAGAGGTTATTGCGTTAACCA
TTAATCATCAGCTACGTGGTGAAGATTCAACAGCCGATCAGAAGTTTGTAGAACAATGGTGCTTTCATTCGGATATAAGA
TGTATTGCTCATCAAGTTGATGTTGGTGAATATCAGCGCCAATACCGGGTAAGTGAAGAGCTTGCTGCCCGTAGATTGAG
ATATGAAATTTATGAAGCAGAGATGAGGAAACAAGGTGCTGACTATCTTGCATTAGGCCATCATGGTGATGACCAAGTAG
AGACCTTGTTTATGCGGTTAACACGTGTAGCTACTTCTAATGCTTTTGAAGGTATTCCGGTAAAGCGTTCCTTTGCAAGT
GGTCAACTTATTCGTCCATTTCTCTGTGTAAACAAACAGCTAATTCTAAACTATGTAAAAGAAAATGAAGTTCCTTTTCG
GGAAGATCAAACGAATAAAGATAATAAATATACAAGGAATTATTATCGTAATGAAATTATTCCACTGCTCACCAAAAATA
ACGAGCGGTTATTTATAACAGCGCAACGTTTAAGTGAAACGTTAAGGGAAGATGAAAACTATTTAGCCAAAGAAGCAAAT
AGAATGGTTGAAGAGGTTATCATTTGGGATGAAAATTTCAGTAAAATAAGTTTCTCCAACCAAGCATTTATCGAACGCCC
CCACGCTTTACAAAGACGTGCCTATCATCTAATATTAAACTATCTGTATGATACATTACCTAAGGATTTATCTTATATTC
ATGAAGAGAAATTTTTTGCATTAATTGAACGACAAGAGGGTAATACTTATATAGATTTCCCTTTATCATTACGTGTAGAA
AATTCGTATGGCAAAATTCAACTATATTTTCCAAATCGACATCCTCGTCATTCTGTATTTCACTTACCGTTGCAAGTCCC
TGATCAAGTGGAATTGCCTGATGGTGCTCGGATTACTTCTGAGTGGATTGATGCTACACTAGATAAAAATAGCCGCAATA
ATATTATTATTCCAATTGATTCTGTAGCGTTGCCATTACATATACGAACGCGAAAACCAGGTGATCGAATGACGTGGGAC
GGCTTAAAAGGAACGAAAAAACTAAAAGATATTTGGATTGACGCAAAAATCCCAACAAATGAAAGGGATACATGGCCTAT
TGTTACAGATGATAATAATACGATTATTTGGTTGGTAGGGCTCAAAAAAGCGTTTGGTTCGAATCAATTTTGTCAAAGTG
GAGAAAAGATAAAACTTAGCTATCATAAAGGGAATATTTAG

Upstream 100 bases:

>100_bases
GTTGTATCGCAACAGTGTATTATCTGCTGTCTCTACTTTTATAGTAAATAGAGGTATGATAGGATAAAGACGACATTTTA
GTGAAGCGGTGGGTAAATCG

Downstream 100 bases:

>100_bases
GAGGATGAATCATGCTTCAAGACAACATACACGGCGATATTCAAGACATTTTAATAACAGAGGAACAAATTCAGGAGAAA
TGTAAAGAACTCGGATCAAT

Product: cell-cycle protein

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase

Number of amino acids: Translated: 466; Mature: 466

Protein sequence:

>466_residues
MKQSILSFIQKHQLLKEGAKIIVGVSGGSDSMALLHFLKNLQEQWEIEVIALTINHQLRGEDSTADQKFVEQWCFHSDIR
CIAHQVDVGEYQRQYRVSEELAARRLRYEIYEAEMRKQGADYLALGHHGDDQVETLFMRLTRVATSNAFEGIPVKRSFAS
GQLIRPFLCVNKQLILNYVKENEVPFREDQTNKDNKYTRNYYRNEIIPLLTKNNERLFITAQRLSETLREDENYLAKEAN
RMVEEVIIWDENFSKISFSNQAFIERPHALQRRAYHLILNYLYDTLPKDLSYIHEEKFFALIERQEGNTYIDFPLSLRVE
NSYGKIQLYFPNRHPRHSVFHLPLQVPDQVELPDGARITSEWIDATLDKNSRNNIIIPIDSVALPLHIRTRKPGDRMTWD
GLKGTKKLKDIWIDAKIPTNERDTWPIVTDDNNTIIWLVGLKKAFGSNQFCQSGEKIKLSYHKGNI

Sequences:

>Translated_466_residues
MKQSILSFIQKHQLLKEGAKIIVGVSGGSDSMALLHFLKNLQEQWEIEVIALTINHQLRGEDSTADQKFVEQWCFHSDIR
CIAHQVDVGEYQRQYRVSEELAARRLRYEIYEAEMRKQGADYLALGHHGDDQVETLFMRLTRVATSNAFEGIPVKRSFAS
GQLIRPFLCVNKQLILNYVKENEVPFREDQTNKDNKYTRNYYRNEIIPLLTKNNERLFITAQRLSETLREDENYLAKEAN
RMVEEVIIWDENFSKISFSNQAFIERPHALQRRAYHLILNYLYDTLPKDLSYIHEEKFFALIERQEGNTYIDFPLSLRVE
NSYGKIQLYFPNRHPRHSVFHLPLQVPDQVELPDGARITSEWIDATLDKNSRNNIIIPIDSVALPLHIRTRKPGDRMTWD
GLKGTKKLKDIWIDAKIPTNERDTWPIVTDDNNTIIWLVGLKKAFGSNQFCQSGEKIKLSYHKGNI
>Mature_466_residues
MKQSILSFIQKHQLLKEGAKIIVGVSGGSDSMALLHFLKNLQEQWEIEVIALTINHQLRGEDSTADQKFVEQWCFHSDIR
CIAHQVDVGEYQRQYRVSEELAARRLRYEIYEAEMRKQGADYLALGHHGDDQVETLFMRLTRVATSNAFEGIPVKRSFAS
GQLIRPFLCVNKQLILNYVKENEVPFREDQTNKDNKYTRNYYRNEIIPLLTKNNERLFITAQRLSETLREDENYLAKEAN
RMVEEVIIWDENFSKISFSNQAFIERPHALQRRAYHLILNYLYDTLPKDLSYIHEEKFFALIERQEGNTYIDFPLSLRVE
NSYGKIQLYFPNRHPRHSVFHLPLQVPDQVELPDGARITSEWIDATLDKNSRNNIIIPIDSVALPLHIRTRKPGDRMTWD
GLKGTKKLKDIWIDAKIPTNERDTWPIVTDDNNTIIWLVGLKKAFGSNQFCQSGEKIKLSYHKGNI

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family

Homologues:

Organism=Escherichia coli, GI1786386, Length=198, Percent_Identity=30.3030303030303, Blast_Score=82, Evalue=7e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TILS_OCEIH (Q8EU18)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_690998.1
- ProteinModelPortal:   Q8EU18
- SMR:   Q8EU18
- GeneID:   1015142
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB0077
- NMPDR:   fig|221109.1.peg.77
- HOGENOM:   HBG668702
- OMA:   NDISYPL
- BioCyc:   OIHE221109:OB0077-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_01161
- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR020825
- InterPro:   IPR014729
- InterPro:   IPR011063
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11807:SF2
- SMART:   SM00977
- TIGRFAMs:   TIGR02433
- TIGRFAMs:   TIGR02432

Pfam domain/function: PF01171 ATP_bind_3; PF11734 TilS_C; SSF56037 B3_4

EC number: NA

Molecular weight: Translated: 54482; Mature: 54482

Theoretical pI: Translated: 7.47; Mature: 7.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQSILSFIQKHQLLKEGAKIIVGVSGGSDSMALLHFLKNLQEQWEIEVIALTINHQLRG
CCHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHCCEEEEEEEECCEECC
EDSTADQKFVEQWCFHSDIRCIAHQVDVGEYQRQYRVSEELAARRLRYEIYEAEMRKQGA
CCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DYLALGHHGDDQVETLFMRLTRVATSNAFEGIPVKRSFASGQLIRPFLCVNKQLILNYVK
CEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHCHHHHHHHHH
ENEVPFREDQTNKDNKYTRNYYRNEIIPLLTKNNERLFITAQRLSETLREDENYLAKEAN
CCCCCCCCCCCCCCCHHHHHHHHCCEEEEEECCCCEEEEEHHHHHHHHHHHHHHHHHHHH
RMVEEVIIWDENFSKISFSNQAFIERPHALQRRAYHLILNYLYDTLPKDLSYIHEEKFFA
HHHHHHEEECCCCCEEECCCCHHHCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHEE
LIERQEGNTYIDFPLSLRVENSYGKIQLYFPNRHPRHSVFHLPLQVPDQVELPDGARITS
EEEECCCCEEEEEEEEEEEECCCCEEEEECCCCCCCCCEEEECCCCCCCCCCCCCCCHHH
EWIDATLDKNSRNNIIIPIDSVALPLHIRTRKPGDRMTWDGLKGTKKLKDIWIDAKIPTN
HHHHHHCCCCCCCCEEEEECCEEEEEEEEECCCCCCCCCCCCCCCCHHHHEEEEEECCCC
ERDTWPIVTDDNNTIIWLVGLKKAFGSNQFCQSGEKIKLSYHKGNI
CCCCCCEEECCCCEEEEEEEEHHHHCCCHHHCCCCEEEEEEECCCC
>Mature Secondary Structure
MKQSILSFIQKHQLLKEGAKIIVGVSGGSDSMALLHFLKNLQEQWEIEVIALTINHQLRG
CCHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHCCEEEEEEEECCEECC
EDSTADQKFVEQWCFHSDIRCIAHQVDVGEYQRQYRVSEELAARRLRYEIYEAEMRKQGA
CCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DYLALGHHGDDQVETLFMRLTRVATSNAFEGIPVKRSFASGQLIRPFLCVNKQLILNYVK
CEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHCHHHHHHHHH
ENEVPFREDQTNKDNKYTRNYYRNEIIPLLTKNNERLFITAQRLSETLREDENYLAKEAN
CCCCCCCCCCCCCCCHHHHHHHHCCEEEEEECCCCEEEEEHHHHHHHHHHHHHHHHHHHH
RMVEEVIIWDENFSKISFSNQAFIERPHALQRRAYHLILNYLYDTLPKDLSYIHEEKFFA
HHHHHHEEECCCCCEEECCCCHHHCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHEE
LIERQEGNTYIDFPLSLRVENSYGKIQLYFPNRHPRHSVFHLPLQVPDQVELPDGARITS
EEEECCCCEEEEEEEEEEEECCCCEEEEECCCCCCCCCEEEECCCCCCCCCCCCCCCHHH
EWIDATLDKNSRNNIIIPIDSVALPLHIRTRKPGDRMTWDGLKGTKKLKDIWIDAKIPTN
HHHHHHCCCCCCCCEEEEECCEEEEEEEEECCCCCCCCCCCCCCCCHHHHEEEEEECCCC
ERDTWPIVTDDNNTIIWLVGLKKAFGSNQFCQSGEKIKLSYHKGNI
CCCCCCEEECCCCEEEEEEEEHHHHCCCHHHCCCCEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12235376