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The map label for this gene is yaaO [H]

Identifier: 23097490

GI number: 23097490

Start: 37465

End: 38883

Strand: Direct

Name: yaaO [H]

Synonym: OB0035

Alternate gene names: 23097490

Gene position: 37465-38883 (Clockwise)

Preceding gene: 23097489

Following gene: 23097491

Centisome position: 1.03

GC content: 37.56

Gene sequence:

>1419_bases
ATGAAGTTGGATCAATCTTATATGCCATTCGTAGATCATTTAAGAAATTTCAGTTCAAAGAGACCGGTATCATTCCATGT
TCCAGGGCATAAGCATGGGGCGGTTTTTCCTGAAGCAGCTAGAGAATTATTTGGCTCTATTTTATCTGTAGATGTAACTG
AAATAGCAGGAATGGATGACTTGCATGCACCAAGTGAGATCATCGCAGAAGCAGAAAAACTAGCAACAGATTATTTCAAT
TCTGACCATACATTTTTTTTAGTTGGAGGATCTACATCAGGTAATCTAGCAATGATTATGGCAGCATGTAATCCGGGAGA
TGAGGTAATCGTCCAACGGAATTGTCATAAATCAATTATGAATGGCTTAGAACTTGCAGGTGCAAAGCCAATATTTATAG
CTCCAGAGTGGAGAGATGATTTACAACGATATACCGCACCAACATATGATAGCATAAAACAAGCAATGTTGAGGTTTCCG
AATGCAAAGGCTCTTATTCTCACCTATCCAGATTACTTTGGGGCGGTTTTTGATATTCAGTCCATGATTGAATTAGCACA
CACCTATCATATTCCAGTATTTGTTGATGAAGCACATGGCGTCCATTTTTCATCATCTGAAATGTTTCCACAATCGGCTT
TACAGTTAGGAGCAGATGTTGTAGTACAATCCGCACATAAAATGGCTCCAGCTATGACAATGGCTGCATATTTACATATT
GGCACTTCACGTGTAGAAAAACATCGACTTGCTTATTATTTGCAAATGTTACAGTCGAGTAGTCCATCTTATCCGATAAT
GGCTTCTTTGGATGTAGCAAGATTTTATTTAGCGCAATTATCAAAAGAAAAAATCCGAGGTATCTTAAATAGTGTAGAAA
ACGTAAGAAACATATTCTCCGGGGCATCATTTTGGGAATTGGTACCTCATGATGATCCGTTAAAATTAACGTTCCACGTG
AAACATGGCTATAAAATAAGTGAGATAGAATCGATGCTCGAATATCATGGAATTTATCCAGAGTTAACGACAGAAAATCA
ATTCTTATTCATACATGGTTTAGCGCCTTTTGTAGAACAAGATTATTTGAAAAAAACAATACAATTGATAGAGGAAGAAT
TAAAAAAATCAGTGAAACATGCTACAATAGAAGGGGCAAAGCTTTTTACCAATCCTATAGAGGAACTAGCATTGTCATAC
CAAGAAATGAAACACATTACATCACTTCTTGTCCCATTTGCTCAAAGTGAAGGTGAAATTGCAGCAGAGGCAGTGATCCC
ATATCCCCCTGGTATTCCAGTCATACTCAAAGGCGAGAGAATTACGAAGGCGCATATTACAAAGATTCAGCTTTTGATTC
AACAAGGAGTAACCATTCAGCAACGGGAAGAAGGAATACGTATATATAGGAAGGCATGA

Upstream 100 bases:

>100_bases
GCAAAGGCTTTTTTTCTGCTGTAAAAAACACTAATCTATCCTTTTTTGACAGGACTTTGTGTTTTTTTTATTGTATATAA
TAGAGGAAATAAGGGAGATG

Downstream 100 bases:

>100_bases
GACACAATATATGGCTGGTTTGTTTATTACATTTGAAGGTGGAGAAGGTGCTGGAAAGACAACAGTTATCCAACATATCT
TCCAAAAATTAATTGCTCAA

Product: lysine decarboxylase

Products: cadaverine; CO2

Alternate protein names: NA

Number of amino acids: Translated: 472; Mature: 472

Protein sequence:

>472_residues
MKLDQSYMPFVDHLRNFSSKRPVSFHVPGHKHGAVFPEAARELFGSILSVDVTEIAGMDDLHAPSEIIAEAEKLATDYFN
SDHTFFLVGGSTSGNLAMIMAACNPGDEVIVQRNCHKSIMNGLELAGAKPIFIAPEWRDDLQRYTAPTYDSIKQAMLRFP
NAKALILTYPDYFGAVFDIQSMIELAHTYHIPVFVDEAHGVHFSSSEMFPQSALQLGADVVVQSAHKMAPAMTMAAYLHI
GTSRVEKHRLAYYLQMLQSSSPSYPIMASLDVARFYLAQLSKEKIRGILNSVENVRNIFSGASFWELVPHDDPLKLTFHV
KHGYKISEIESMLEYHGIYPELTTENQFLFIHGLAPFVEQDYLKKTIQLIEEELKKSVKHATIEGAKLFTNPIEELALSY
QEMKHITSLLVPFAQSEGEIAAEAVIPYPPGIPVILKGERITKAHITKIQLLIQQGVTIQQREEGIRIYRKA

Sequences:

>Translated_472_residues
MKLDQSYMPFVDHLRNFSSKRPVSFHVPGHKHGAVFPEAARELFGSILSVDVTEIAGMDDLHAPSEIIAEAEKLATDYFN
SDHTFFLVGGSTSGNLAMIMAACNPGDEVIVQRNCHKSIMNGLELAGAKPIFIAPEWRDDLQRYTAPTYDSIKQAMLRFP
NAKALILTYPDYFGAVFDIQSMIELAHTYHIPVFVDEAHGVHFSSSEMFPQSALQLGADVVVQSAHKMAPAMTMAAYLHI
GTSRVEKHRLAYYLQMLQSSSPSYPIMASLDVARFYLAQLSKEKIRGILNSVENVRNIFSGASFWELVPHDDPLKLTFHV
KHGYKISEIESMLEYHGIYPELTTENQFLFIHGLAPFVEQDYLKKTIQLIEEELKKSVKHATIEGAKLFTNPIEELALSY
QEMKHITSLLVPFAQSEGEIAAEAVIPYPPGIPVILKGERITKAHITKIQLLIQQGVTIQQREEGIRIYRKA
>Mature_472_residues
MKLDQSYMPFVDHLRNFSSKRPVSFHVPGHKHGAVFPEAARELFGSILSVDVTEIAGMDDLHAPSEIIAEAEKLATDYFN
SDHTFFLVGGSTSGNLAMIMAACNPGDEVIVQRNCHKSIMNGLELAGAKPIFIAPEWRDDLQRYTAPTYDSIKQAMLRFP
NAKALILTYPDYFGAVFDIQSMIELAHTYHIPVFVDEAHGVHFSSSEMFPQSALQLGADVVVQSAHKMAPAMTMAAYLHI
GTSRVEKHRLAYYLQMLQSSSPSYPIMASLDVARFYLAQLSKEKIRGILNSVENVRNIFSGASFWELVPHDDPLKLTFHV
KHGYKISEIESMLEYHGIYPELTTENQFLFIHGLAPFVEQDYLKKTIQLIEEELKKSVKHATIEGAKLFTNPIEELALSY
QEMKHITSLLVPFAQSEGEIAAEAVIPYPPGIPVILKGERITKAHITKIQLLIQQGVTIQQREEGIRIYRKA

Specific function: Ldc Is Constitutively But Weakly Expressed Under Various Conditions. [C]

COG id: COG1982

COG function: function code E; Arginine/lysine/ornithine decarboxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Orn/Lys/Arg decarboxylase class-I family [H]

Homologues:

Organism=Escherichia coli, GI1786384, Length=564, Percent_Identity=21.8085106382979, Blast_Score=92, Evalue=9e-20,
Organism=Escherichia coli, GI221142684, Length=301, Percent_Identity=25.5813953488372, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI87082193, Length=299, Percent_Identity=26.4214046822742, Blast_Score=73, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000310
- InterPro:   IPR008286
- InterPro:   IPR015424
- InterPro:   IPR015421 [H]

Pfam domain/function: PF01276 OKR_DC_1; PF03711 OKR_DC_1_C [H]

EC number: 4.1.1.18

Molecular weight: Translated: 52989; Mature: 52989

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLDQSYMPFVDHLRNFSSKRPVSFHVPGHKHGAVFPEAARELFGSILSVDVTEIAGMDD
CCCCCCCCHHHHHHHCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCC
LHAPSEIIAEAEKLATDYFNSDHTFFLVGGSTSGNLAMIMAACNPGDEVIVQRNCHKSIM
CCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCEEEEEEECCCCCCEEEECHHHHHHH
NGLELAGAKPIFIAPEWRDDLQRYTAPTYDSIKQAMLRFPNAKALILTYPDYFGAVFDIQ
HHHHHCCCCEEEECCCHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHH
SMIELAHTYHIPVFVDEAHGVHFSSSEMFPQSALQLGADVVVQSAHKMAPAMTMAAYLHI
HHHHHHHHCCCEEEEECCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GTSRVEKHRLAYYLQMLQSSSPSYPIMASLDVARFYLAQLSKEKIRGILNSVENVRNIFS
CHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GASFWELVPHDDPLKLTFHVKHGYKISEIESMLEYHGIYPELTTENQFLFIHGLAPFVEQ
CCCEEEECCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEECCCHHHHH
DYLKKTIQLIEEELKKSVKHATIEGAKLFTNPIEELALSYQEMKHITSLLVPFAQSEGEI
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
AAEAVIPYPPGIPVILKGERITKAHITKIQLLIQQGVTIQQREEGIRIYRKA
EEEEECCCCCCCCEEEECCEECHHHHHHHHHHHHHCCCHHHHHCCCEEEECC
>Mature Secondary Structure
MKLDQSYMPFVDHLRNFSSKRPVSFHVPGHKHGAVFPEAARELFGSILSVDVTEIAGMDD
CCCCCCCCHHHHHHHCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCC
LHAPSEIIAEAEKLATDYFNSDHTFFLVGGSTSGNLAMIMAACNPGDEVIVQRNCHKSIM
CCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCEEEEEEECCCCCCEEEECHHHHHHH
NGLELAGAKPIFIAPEWRDDLQRYTAPTYDSIKQAMLRFPNAKALILTYPDYFGAVFDIQ
HHHHHCCCCEEEECCCHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHH
SMIELAHTYHIPVFVDEAHGVHFSSSEMFPQSALQLGADVVVQSAHKMAPAMTMAAYLHI
HHHHHHHHCCCEEEEECCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GTSRVEKHRLAYYLQMLQSSSPSYPIMASLDVARFYLAQLSKEKIRGILNSVENVRNIFS
CHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GASFWELVPHDDPLKLTFHVKHGYKISEIESMLEYHGIYPELTTENQFLFIHGLAPFVEQ
CCCEEEECCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEECCCHHHHH
DYLKKTIQLIEEELKKSVKHATIEGAKLFTNPIEELALSYQEMKHITSLLVPFAQSEGEI
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
AAEAVIPYPPGIPVILKGERITKAHITKIQLLIQQGVTIQQREEGIRIYRKA
EEEEECCCCCCCCEEEECCEECHHHHHHHHHHHHHCCCHHHHHCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Pyridoxal 5'-phosphate; Sulfhydryl groups [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 3.4 {S-aminoethyl-L-Cys}} 2 {L-Lys}} 1.5 {L-Lys}} 7 {delta-hydroxylysine}} [C]

Substrates: L-lysine

Specific reaction: L-lysine = cadaverine + CO2

General reaction: Decarboxylation [C]

Inhibitor: NaBH4; NaCl [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]