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The map label for this gene is gyrB [H]

Identifier: 23097461

GI number: 23097461

Start: 5109

End: 7034

Strand: Direct

Name: gyrB [H]

Synonym: OB0006

Alternate gene names: 23097461

Gene position: 5109-7034 (Clockwise)

Preceding gene: 23097460

Following gene: 23097462

Centisome position: 0.14

GC content: 38.01

Gene sequence:

>1926_bases
ATGTCTATGGAAGATAAAATTACGGAAAACCAAGAATATGGTGCAGATCAGATACAAGTTCTTGAAGGATTAGAAGCAGT
TAGAAAAAGACCTGGTATGTATATTGGCTCTACTAGTGAAAAAGGGCTTCATCACTTAGTCTGGGAAATTGTCGATAATA
GTATCGATGAAGCATTAGCAGGTTATTGTGACCATATTCAAGTAGTGGTTGAAGAAGATAATAGTATTACAGTAAAGGAT
AATGGACGTGGTATTCCAGTTGATATCCAACAAAAAACTGGAAGACCCGCACTTGAAGTAATTATGACTGTACTACATGC
TGGCGGAAAATTCGGTGGAGGCGGTTATAAGGTTTCTGGCGGATTACACGGTGTAGGGGCATCGGTTGTAAATGCACTTT
CTAGTGAATTAGAAGTGTATGTACATCGTGATGGGAAAGTTCATTTCCTGTCGTTCAAAAAAGGTGTTCCAGATGGAGAA
ATTAAGGTGATTGGAGATACCGATATTACTGGTACTGTAACTCACTTTAGACCAGATACAGAAATCTTCACAGAAACAAC
AGAATATAACTTTGATACATTGGAACAACGTCTTCGTGAGCTTGCATTTTTAAATAAAGGATTAAAAATTTCTATCGAAG
ATAAACGTACGGATAGAGAACAAGTGACGTACCACTATGAAGGCGGTATAAGTTCTTATGTTGAATTTATTAATAAAAAT
AAAGAAGTTCTTCATGAGCCCTTCTTTGCAGAAGGAGAAGACCAAGGGATCTCTGTAGAAGTAGCTATTCAATACAATGA
TGGATTTGCAAGCAATTTATACTCATTCGCGAATAATATCCATACGTATGAAGGTGGATCACATGAAGTCGGATTCCGTA
GCGGTTTGACACGTATAATAAATGATTATGCAAAGAAAAACGGTTTAATTAAGGATGGAGATTCTAATCTTTCCGGTGAC
GATGTTCGTGAAGGAATGACAACAATCGTATCTATCAAACATCCAGATCCACAATTTGAAGGACAAACAAAGACGAAACT
CGGTAATAGTGAAGTTCGTGCAATAACAGATGGAGTCTTTTCTGAAGCATTTTCTAAGTTTTTATATGAAAATCCTTCCA
CAGCAAAAATTATTGTCGAAAAAGGATTGATGGCGTCACGAGCACGGCTTGCAGCTAAAAAAGCAAGAGAACTTACACGT
CGTAAAAGTAACTTAGAGATTTCTAATTTACCTGGTAAGTTAGCTGACTGTTCTTCACGAGATGCCGCTATAAGTGAACT
ATATATTGTAGAGGGGGACTCAGCTGGAGGTTCTGCAAAATCCGGTAGGGATCGCCATTTCCAAGCAATCCTCCCACTAA
GAGGTAAGATTTTAAACGTGGAAAAAGCACGCTTGGACCGTATTTTATCTAATAATGAAGTACGAGCTATGATAACAGCG
CTAGGAAGCGGTGTTGGCGAAGAATTCGATATTAGTAAAGCACGATATCACAAAATCGTTATCATGACGGATGCTGACGT
AGATGGTGCGCATATTCGAACATTGCTATTAACGTTCTTCTACCGATATATGCGTCCACTGATTGAACAAGGCTATATTT
ACATCGCGCAACCACCACTTTATCAAGTGAAGCAAGGAAAAACGGTGAATTATGCGTATAACGATAAAGAATTGGATCGG
ATATTAAATGAAATTCCTAAAGCACCAAAACCTAATATTCAGCGATACAAAGGTTTAGGAGAAATGAACGCAGATCAATT
ATGGGATACAACAATGGACCCTGATACTCGTACACTCCTGCAAGTAGAATTAAGCGATGCAATCGATGCTGACCAAGTAT
TCGATATGTTAATGGGAGATAAAGTAGAGCCACGTCGAATCTTTATCGAAGAAAATGCACAATATGTGAAGAACTTAGAC
ATTTAA

Upstream 100 bases:

>100_bases
GCTAAATCAAGTTATTTATTGCAAAAGCTTCCGAATAGAAGGACATCGATAAAATCGGTCTCCTATTTAAGTGTATTTCA
ACAGAAATGAGTGTGAGAAA

Downstream 100 bases:

>100_bases
CCATGTCCAAATATGAATGATTAGATAGAAGATCGATTGAAGGAAATCACTAAGTTAGTGTGCTTAGTGATTGGGAGGTA
ATGAAAGAATGGCAGATATA

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 641; Mature: 640

Protein sequence:

>641_residues
MSMEDKITENQEYGADQIQVLEGLEAVRKRPGMYIGSTSEKGLHHLVWEIVDNSIDEALAGYCDHIQVVVEEDNSITVKD
NGRGIPVDIQQKTGRPALEVIMTVLHAGGKFGGGGYKVSGGLHGVGASVVNALSSELEVYVHRDGKVHFLSFKKGVPDGE
IKVIGDTDITGTVTHFRPDTEIFTETTEYNFDTLEQRLRELAFLNKGLKISIEDKRTDREQVTYHYEGGISSYVEFINKN
KEVLHEPFFAEGEDQGISVEVAIQYNDGFASNLYSFANNIHTYEGGSHEVGFRSGLTRIINDYAKKNGLIKDGDSNLSGD
DVREGMTTIVSIKHPDPQFEGQTKTKLGNSEVRAITDGVFSEAFSKFLYENPSTAKIIVEKGLMASRARLAAKKARELTR
RKSNLEISNLPGKLADCSSRDAAISELYIVEGDSAGGSAKSGRDRHFQAILPLRGKILNVEKARLDRILSNNEVRAMITA
LGSGVGEEFDISKARYHKIVIMTDADVDGAHIRTLLLTFFYRYMRPLIEQGYIYIAQPPLYQVKQGKTVNYAYNDKELDR
ILNEIPKAPKPNIQRYKGLGEMNADQLWDTTMDPDTRTLLQVELSDAIDADQVFDMLMGDKVEPRRIFIEENAQYVKNLD
I

Sequences:

>Translated_641_residues
MSMEDKITENQEYGADQIQVLEGLEAVRKRPGMYIGSTSEKGLHHLVWEIVDNSIDEALAGYCDHIQVVVEEDNSITVKD
NGRGIPVDIQQKTGRPALEVIMTVLHAGGKFGGGGYKVSGGLHGVGASVVNALSSELEVYVHRDGKVHFLSFKKGVPDGE
IKVIGDTDITGTVTHFRPDTEIFTETTEYNFDTLEQRLRELAFLNKGLKISIEDKRTDREQVTYHYEGGISSYVEFINKN
KEVLHEPFFAEGEDQGISVEVAIQYNDGFASNLYSFANNIHTYEGGSHEVGFRSGLTRIINDYAKKNGLIKDGDSNLSGD
DVREGMTTIVSIKHPDPQFEGQTKTKLGNSEVRAITDGVFSEAFSKFLYENPSTAKIIVEKGLMASRARLAAKKARELTR
RKSNLEISNLPGKLADCSSRDAAISELYIVEGDSAGGSAKSGRDRHFQAILPLRGKILNVEKARLDRILSNNEVRAMITA
LGSGVGEEFDISKARYHKIVIMTDADVDGAHIRTLLLTFFYRYMRPLIEQGYIYIAQPPLYQVKQGKTVNYAYNDKELDR
ILNEIPKAPKPNIQRYKGLGEMNADQLWDTTMDPDTRTLLQVELSDAIDADQVFDMLMGDKVEPRRIFIEENAQYVKNLD
I
>Mature_640_residues
SMEDKITENQEYGADQIQVLEGLEAVRKRPGMYIGSTSEKGLHHLVWEIVDNSIDEALAGYCDHIQVVVEEDNSITVKDN
GRGIPVDIQQKTGRPALEVIMTVLHAGGKFGGGGYKVSGGLHGVGASVVNALSSELEVYVHRDGKVHFLSFKKGVPDGEI
KVIGDTDITGTVTHFRPDTEIFTETTEYNFDTLEQRLRELAFLNKGLKISIEDKRTDREQVTYHYEGGISSYVEFINKNK
EVLHEPFFAEGEDQGISVEVAIQYNDGFASNLYSFANNIHTYEGGSHEVGFRSGLTRIINDYAKKNGLIKDGDSNLSGDD
VREGMTTIVSIKHPDPQFEGQTKTKLGNSEVRAITDGVFSEAFSKFLYENPSTAKIIVEKGLMASRARLAAKKARELTRR
KSNLEISNLPGKLADCSSRDAAISELYIVEGDSAGGSAKSGRDRHFQAILPLRGKILNVEKARLDRILSNNEVRAMITAL
GSGVGEEFDISKARYHKIVIMTDADVDGAHIRTLLLTFFYRYMRPLIEQGYIYIAQPPLYQVKQGKTVNYAYNDKELDRI
LNEIPKAPKPNIQRYKGLGEMNADQLWDTTMDPDTRTLLQVELSDAIDADQVFDMLMGDKVEPRRIFIEENAQYVKNLDI

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=625, Percent_Identity=27.2, Blast_Score=129, Evalue=1e-29,
Organism=Homo sapiens, GI19913406, Length=619, Percent_Identity=26.4943457189015, Blast_Score=126, Evalue=7e-29,
Organism=Escherichia coli, GI48994957, Length=551, Percent_Identity=56.2613430127042, Blast_Score=619, Evalue=1e-178,
Organism=Escherichia coli, GI1789408, Length=629, Percent_Identity=41.0174880763116, Blast_Score=463, Evalue=1e-131,
Organism=Caenorhabditis elegans, GI17535065, Length=656, Percent_Identity=26.5243902439024, Blast_Score=144, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI212645845, Length=599, Percent_Identity=23.7061769616027, Blast_Score=124, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI212645657, Length=209, Percent_Identity=27.7511961722488, Blast_Score=75, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6324241, Length=632, Percent_Identity=25.1582278481013, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI17136538, Length=672, Percent_Identity=24.702380952381, Blast_Score=129, Evalue=6e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR006171 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 71498; Mature: 71366

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMEDKITENQEYGADQIQVLEGLEAVRKRPGMYIGSTSEKGLHHLVWEIVDNSIDEALA
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHH
GYCDHIQVVVEEDNSITVKDNGRGIPVDIQQKTGRPALEVIMTVLHAGGKFGGGGYKVSG
HHCCEEEEEEECCCEEEEEECCCCCCEEECCCCCCHHHHHHHHHHHCCCCCCCCCEEECC
GLHGVGASVVNALSSELEVYVHRDGKVHFLSFKKGVPDGEIKVIGDTDITGTVTHFRPDT
CCCCHHHHHHHHHHHCEEEEEECCCCEEEEEECCCCCCCCEEEEECCCCCEEEEEECCCC
EIFTETTEYNFDTLEQRLRELAFLNKGLKISIEDKRTDREQVTYHYEGGISSYVEFINKN
HHEECCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHEEEEECCCHHHHHHHHHCC
KEVLHEPFFAEGEDQGISVEVAIQYNDGFASNLYSFANNIHTYEGGSHEVGFRSGLTRII
HHHHHCCCCCCCCCCCEEEEEEEEECCCHHHHHHHHHHCCEECCCCCCCCHHHHHHHHHH
NDYAKKNGLIKDGDSNLSGDDVREGMTTIVSIKHPDPQFEGQTKTKLGNSEVRAITDGVF
HHHHHHCCCEECCCCCCCCHHHHCCCEEEEEEECCCCCCCCCHHHCCCCCHHHHHHHHHH
SEAFSKFLYENPSTAKIIVEKGLMASRARLAAKKARELTRRKSNLEISNLPGKLADCSSR
HHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCC
DAAISELYIVEGDSAGGSAKSGRDRHFQAILPLRGKILNVEKARLDRILSNNEVRAMITA
CCEEEEEEEEECCCCCCCCCCCCCCCEEEEECCCCEEECHHHHHHHHHHCCCHHHHHHHH
LGSGVGEEFDISKARYHKIVIMTDADVDGAHIRTLLLTFFYRYMRPLIEQGYIYIAQPPL
HHCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCC
YQVKQGKTVNYAYNDKELDRILNEIPKAPKPNIQRYKGLGEMNADQLWDTTMDPDTRTLL
HHHCCCCEEEEEECHHHHHHHHHHCCCCCCCCHHHHCCCCCCCHHHHCCCCCCCCCCEEE
QVELSDAIDADQVFDMLMGDKVEPRRIFIEENAQYVKNLDI
EEEHHHCCCHHHHHHHHHCCCCCCCEEEEECCHHHHHCCCC
>Mature Secondary Structure 
SMEDKITENQEYGADQIQVLEGLEAVRKRPGMYIGSTSEKGLHHLVWEIVDNSIDEALA
CCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHH
GYCDHIQVVVEEDNSITVKDNGRGIPVDIQQKTGRPALEVIMTVLHAGGKFGGGGYKVSG
HHCCEEEEEEECCCEEEEEECCCCCCEEECCCCCCHHHHHHHHHHHCCCCCCCCCEEECC
GLHGVGASVVNALSSELEVYVHRDGKVHFLSFKKGVPDGEIKVIGDTDITGTVTHFRPDT
CCCCHHHHHHHHHHHCEEEEEECCCCEEEEEECCCCCCCCEEEEECCCCCEEEEEECCCC
EIFTETTEYNFDTLEQRLRELAFLNKGLKISIEDKRTDREQVTYHYEGGISSYVEFINKN
HHEECCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHEEEEECCCHHHHHHHHHCC
KEVLHEPFFAEGEDQGISVEVAIQYNDGFASNLYSFANNIHTYEGGSHEVGFRSGLTRII
HHHHHCCCCCCCCCCCEEEEEEEEECCCHHHHHHHHHHCCEECCCCCCCCHHHHHHHHHH
NDYAKKNGLIKDGDSNLSGDDVREGMTTIVSIKHPDPQFEGQTKTKLGNSEVRAITDGVF
HHHHHHCCCEECCCCCCCCHHHHCCCEEEEEEECCCCCCCCCHHHCCCCCHHHHHHHHHH
SEAFSKFLYENPSTAKIIVEKGLMASRARLAAKKARELTRRKSNLEISNLPGKLADCSSR
HHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCC
DAAISELYIVEGDSAGGSAKSGRDRHFQAILPLRGKILNVEKARLDRILSNNEVRAMITA
CCEEEEEEEEECCCCCCCCCCCCCCCEEEEECCCCEEECHHHHHHHHHHCCCHHHHHHHH
LGSGVGEEFDISKARYHKIVIMTDADVDGAHIRTLLLTFFYRYMRPLIEQGYIYIAQPPL
HHCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCC
YQVKQGKTVNYAYNDKELDRILNEIPKAPKPNIQRYKGLGEMNADQLWDTTMDPDTRTLL
HHHCCCCEEEEEECHHHHHHHHHHCCCCCCCCHHHHCCCCCCCHHHHCCCCCCCCCCEEE
QVELSDAIDADQVFDMLMGDKVEPRRIFIEENAQYVKNLDI
EEEHHHCCCHHHHHHHHHCCCCCCCEEEEECCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2987847; 7584024; 9384377 [H]