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The map label for this gene is ansA [H]

Identifier: 229918695

GI number: 229918695

Start: 2946110

End: 2947081

Strand: Reverse

Name: ansA [H]

Synonym: EAT1b_2982

Alternate gene names: 229918695

Gene position: 2947081-2946110 (Counterclockwise)

Preceding gene: 229918723

Following gene: 229918694

Centisome position: 98.24

GC content: 49.38

Gene sequence:

>972_bases
ATGAATCATTTATTACTGATCCACACCGGCGGTACCATCGCCATGTCACAATCCGGCACAGGCCACGTCGAACCGAGCGA
CGTCAATCCAATCGACGCCACCCTTCCGAAAGCGACGGATATTGCTCACATTACGACAAAACACTTTTCAAATATCCCGT
CACCTCACATGACCCCAGAGAAAATGCTTGAATTGGCACAGTTCATCCAACGTGAACTGACACAAGACACGTATGACGGA
GTCGTCATCACGCACGGTACCGATACATTAGAAGAGACGGCTTACTTCTTACAAATCACGCTCGGCGCTCCTGTCCCAAT
CGTGTTGACAGGTGCGATGCGCTCCTCGAACGAGGTCGGTTCGGACGGGGAATTTAATTTGATCACAGCGCTTCGCGTCG
CCAAGAGCGAAGCGGCTCGTGGAAAAGGTGTGCTCGTCGTCTTCAACGGTGAGATCCATTCGGCATTCAACGTGACTAAA
ACGCACACATCTTCAGTCGACACATTCAAGTCTGTACACTTTGGTAACGTGGGTATGGTCACAAAGGACCACGTACTCAT
CTATAGCCAACCGGCGACGAGACAGCCGAAGATGATTTCGTCCATTTCAAAACGTGTAGCCGTATTGAAGGTCGTCGCGG
GCATGGAACCGGATTTACTCGAATCTGTAATCACGCTTGGATACGACGGACTCGTCCTCGAGGTGTTAGGACAAGGGAAT
GTTCCGCCGTCCCTCTTATCCGCACTCGAACATTTAGTCAAAGAGATTCCCGTCGTAATCGTCAGCCGTTGCTTCAACGG
AATCGTTCAAGATGTTTACGGTTACGATGGGGGTGGCAAACAACTGAAAGAGATGGGTGTCATTTTTTCAAACGGATTGA
ATTCACAAAAAGCTCGTTTACGACTTCTCGTTGAACTCGAGTCCGGCTCATCCCAGTCAGTCATGGAACATAGTTTTAGT
TTCCAGCAATAA

Upstream 100 bases:

>100_bases
CTCTCCTTTCTCTCCCTAGCATACACTGAATTGTCTGAAAGAAAAAGTAGAGAACGCCTAGAAAATGGAAATCTTCATAA
AAATAATTAGGAGGACACTT

Downstream 100 bases:

>100_bases
CCTTGCGCAACTGCGCCCCCTTCCCTACAATGAGCAAAGAGGTGATATTCAGTGAACAAACAAGCGATTATTGTAGGTGC
CGGGCCATGTGGCCTCTCGG

Product: Asparaginase/glutaminase

Products: NA

Alternate protein names: L-ASNase; L-asparagine amidohydrolase [H]

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MNHLLLIHTGGTIAMSQSGTGHVEPSDVNPIDATLPKATDIAHITTKHFSNIPSPHMTPEKMLELAQFIQRELTQDTYDG
VVITHGTDTLEETAYFLQITLGAPVPIVLTGAMRSSNEVGSDGEFNLITALRVAKSEAARGKGVLVVFNGEIHSAFNVTK
THTSSVDTFKSVHFGNVGMVTKDHVLIYSQPATRQPKMISSISKRVAVLKVVAGMEPDLLESVITLGYDGLVLEVLGQGN
VPPSLLSALEHLVKEIPVVIVSRCFNGIVQDVYGYDGGGKQLKEMGVIFSNGLNSQKARLRLLVELESGSSQSVMEHSFS
FQQ

Sequences:

>Translated_323_residues
MNHLLLIHTGGTIAMSQSGTGHVEPSDVNPIDATLPKATDIAHITTKHFSNIPSPHMTPEKMLELAQFIQRELTQDTYDG
VVITHGTDTLEETAYFLQITLGAPVPIVLTGAMRSSNEVGSDGEFNLITALRVAKSEAARGKGVLVVFNGEIHSAFNVTK
THTSSVDTFKSVHFGNVGMVTKDHVLIYSQPATRQPKMISSISKRVAVLKVVAGMEPDLLESVITLGYDGLVLEVLGQGN
VPPSLLSALEHLVKEIPVVIVSRCFNGIVQDVYGYDGGGKQLKEMGVIFSNGLNSQKARLRLLVELESGSSQSVMEHSFS
FQQ
>Mature_323_residues
MNHLLLIHTGGTIAMSQSGTGHVEPSDVNPIDATLPKATDIAHITTKHFSNIPSPHMTPEKMLELAQFIQRELTQDTYDG
VVITHGTDTLEETAYFLQITLGAPVPIVLTGAMRSSNEVGSDGEFNLITALRVAKSEAARGKGVLVVFNGEIHSAFNVTK
THTSSVDTFKSVHFGNVGMVTKDHVLIYSQPATRQPKMISSISKRVAVLKVVAGMEPDLLESVITLGYDGLVLEVLGQGN
VPPSLLSALEHLVKEIPVVIVSRCFNGIVQDVYGYDGGGKQLKEMGVIFSNGLNSQKARLRLLVELESGSSQSVMEHSFS
FQQ

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Homo sapiens, GI122937331, Length=305, Percent_Identity=27.5409836065574, Blast_Score=68, Evalue=9e-12,
Organism=Escherichia coli, GI1789327, Length=294, Percent_Identity=34.3537414965986, Blast_Score=146, Evalue=2e-36,
Organism=Escherichia coli, GI1788065, Length=317, Percent_Identity=28.0757097791798, Blast_Score=85, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI17558280, Length=279, Percent_Identity=26.5232974910394, Blast_Score=75, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17558278, Length=279, Percent_Identity=26.5232974910394, Blast_Score=75, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI193207374, Length=279, Percent_Identity=26.5232974910394, Blast_Score=75, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI193207372, Length=360, Percent_Identity=24.4444444444444, Blast_Score=74, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6323189, Length=332, Percent_Identity=31.6265060240964, Blast_Score=140, Evalue=3e-34,
Organism=Saccharomyces cerevisiae, GI6323187, Length=332, Percent_Identity=31.6265060240964, Blast_Score=140, Evalue=3e-34,
Organism=Saccharomyces cerevisiae, GI6323186, Length=332, Percent_Identity=31.6265060240964, Blast_Score=140, Evalue=3e-34,
Organism=Saccharomyces cerevisiae, GI6323184, Length=332, Percent_Identity=31.6265060240964, Blast_Score=140, Evalue=3e-34,
Organism=Saccharomyces cerevisiae, GI6320527, Length=331, Percent_Identity=30.2114803625378, Blast_Score=136, Evalue=5e-33,
Organism=Drosophila melanogaster, GI24645530, Length=330, Percent_Identity=27.5757575757576, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24639609, Length=247, Percent_Identity=27.1255060728745, Blast_Score=68, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 34845; Mature: 34845

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS00144 ASN_GLN_ASE_1 ; PS00917 ASN_GLN_ASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNHLLLIHTGGTIAMSQSGTGHVEPSDVNPIDATLPKATDIAHITTKHFSNIPSPHMTPE
CCCEEEEECCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCHH
KMLELAQFIQRELTQDTYDGVVITHGTDTLEETAYFLQITLGAPVPIVLTGAMRSSNEVG
HHHHHHHHHHHHHCCHHHCCEEEECCCCCHHHCEEEEEEEECCCCCEEEECCCCCCCCCC
SDGEFNLITALRVAKSEAARGKGVLVVFNGEIHSAFNVTKTHTSSVDTFKSVHFGNVGMV
CCCCEEHHHHHHHHHHHHCCCCCEEEEECCCCCCEEEEEECCCCCHHHHHHCCCCCCCEE
TKDHVLIYSQPATRQPKMISSISKRVAVLKVVAGMEPDLLESVITLGYDGLVLEVLGQGN
EECCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCEEEEEECCCC
VPPSLLSALEHLVKEIPVVIVSRCFNGIVQDVYGYDGGGKQLKEMGVIFSNGLNSQKARL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCEEE
RLLVELESGSSQSVMEHSFSFQQ
EEEEEECCCCCHHHHHHHCCCCC
>Mature Secondary Structure
MNHLLLIHTGGTIAMSQSGTGHVEPSDVNPIDATLPKATDIAHITTKHFSNIPSPHMTPE
CCCEEEEECCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCHH
KMLELAQFIQRELTQDTYDGVVITHGTDTLEETAYFLQITLGAPVPIVLTGAMRSSNEVG
HHHHHHHHHHHHHCCHHHCCEEEECCCCCHHHCEEEEEEEECCCCCEEEECCCCCCCCCC
SDGEFNLITALRVAKSEAARGKGVLVVFNGEIHSAFNVTKTHTSSVDTFKSVHFGNVGMV
CCCCEEHHHHHHHHHHHHCCCCCEEEEECCCCCCEEEEEECCCCCHHHHHHCCCCCCCEE
TKDHVLIYSQPATRQPKMISSISKRVAVLKVVAGMEPDLLESVITLGYDGLVLEVLGQGN
EECCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCEEEEEECCCC
VPPSLLSALEHLVKEIPVVIVSRCFNGIVQDVYGYDGGGKQLKEMGVIFSNGLNSQKARL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCEEE
RLLVELESGSSQSVMEHSFSFQQ
EEEEEECCCCCHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10567266 [H]