Click here to switch to the map view.

The map label for this gene is pnp

Identifier: 229918674

GI number: 229918674

Start: 2920843

End: 2923059

Strand: Direct

Name: pnp

Synonym: EAT1b_2961

Alternate gene names: 229918674

Gene position: 2920843-2923059 (Clockwise)

Preceding gene: 229918673

Following gene: 229918675

Centisome position: 97.36

GC content: 51.01

Gene sequence:

>2217_bases
ATGTTAGGTACGAAACAAGTATTCTCTACAGAGTGGGGCGGACGCCCATTGTCTGTAGAGGTCGGCCAACTTGCGAAGCA
AGCAAACGGTTCGGCACTCGTGCGTTATGGAGATACGGTTGTCCTTGCGACAGTTACGGCTTCAAAAGCACCAAAAGATT
TAGACTTCTTCCCGTTGACGGTCAACTATGAGGAGAAACTTTACTCGGTCGGTAAAATTCCAGGTGGTTTCCTTCGCCGT
GAAGGTCGCCCGGGTGAAAACGCGATTTTGACATCACGTCTCATCGACCGCCCGATTCGTCCACTCTTCCCAGACGGATT
CCGTCATGATATTCAAGTCATGATTTACGTCATGTCAAACGACCCAGATTGCTCGGCTGAAATGGCAGGTATGCTCGGAA
CGTCAATCGCGCTCTCGATTTCTGACGTACCGTTTGACGGTCCAATCGCTGGCGTGACAGTCGGCCGTGTGGACGGCGAA
TTCGTCATCAACCCGACGACGGCTCAGTTGGAGAAAACGGACCTTGAACTTCAAGTTGCAGGGACATCGACAGCCATCAA
CATGGTTGAAGCAGGTGCGAACGAAGTACCTGAGGACGTCATGCTCGAGTCGATCTTGTTTGGACATGAAGAAATCAAGC
GTTTGATTGCATTCCAACAAGAAATCGTAGAAGCTGTCGGAAAACCTAAATTCGAATACACGGTCTCGAAATACGATGAG
GCACTTACTGCTGAACTCGAAGCAGCCCGTCCTGAAATCGTAGAAGCGGTTCAAGTGGAAGAGAAACACGCTCGTGACGA
AGCCATCAACGAAGTGATTGCGAAGTATGTCGCGATGTATGAAGCGCGTCTTGCGGATGAGCCTTCAAAACTCGGCGAAG
TTTCAGGCATCTTGAACAAGTTCGTCAAAGATGAAGTCCGTCGCTTGATCACGGTCGAAAAAGTCCGCCCAGACGGACGT
CGTCCAGACGTGATTCGCCCGCTCGCATCAGAAGTCGGATTACTCCCACGTGCACACGGTGTCGGTCTCTTCACACGAGG
TCAAACACAAGTCATGTCTGTTGCGACACTCGGTGTCATCGGAGACGCACAAATCATCGATGGAATCGGTCTTGAAGAGA
ACAAGCGCTTTATGCACCACTATAACTTCCCGCCGTTCTCAGTCGGTGAAGCTCGTCCGGTCCGTGCGCCAGGTCGTCGT
GAGATCGGTCACGGAGCACTCGGTGAGCGTGCGCTTCTTCCAATCATTCCGAAAGAAGCAGACTTCCCATACACGATTCG
TCTCGTTTCGGAAGTACTCGAATCAAACGGTTCAAGTTCACAAGCTTCGATTTGTGGGTCGACACTCGCATTGATGGATG
CAGGTGTGCCAATCAAAGCGCCAGTCGCTGGGATTGCCATGGGTCTTATCATGGAAGGTGAACACTACACGGTCTTGACA
GATATTCAAGGACTTGAAGACCACCTTGGGGATATGGACTTCAAAGTAGCTGGAACGAAAGACGGGATCACCGCCCTTCA
AATGGATATCAAAATTGCCGGGATCACGCGTGAAATTTTAGAAGAAGCGCTTGAACAGGCACGTGTCGGTCGTTTACACA
TCCTTGACCACATGCTTGAGACGTTAGAGACTCCACGTACACAATTGTCGAAATATGCACCGAAGATTGTGACGATGACA
ATCAATCCGGATAAGATCCGCGACGTCATCGGACCAGGCGGTAAGATGATCAACAGCATCATCGACCAAACGGGCGTCAA
AATCGACATCGAACAAGATGGTACGGTCTTCATCGCCTCGACGGACCAAGAAGGAATCGATCTTGCCATGTCAATGATCG
GTGACATCGTCCGTGAAGTCGTCGTCGGTGAAGTATACGATGCTACCGTACGTCGTATCGAAAAATTCGGCGCGTTCGTC
GAGTTGTTCAAAGGTAAAGATGCGCTCGTACACGTGTCCGAATTCAGCCTTGAGCGTGTCGCGAACGTCGAGGATGTTGT
GAAGCTCGGCGATTCGATTCAAGTCAAAGTCACTGAAATCGATGACAAAGGTCGTGTCAACGCATCACGAAAAGCGCTCA
TCTTAGAAGGATTGTCTCCTGAAGAGCGTGAGGCATACGAAGCGAAACGGAAAGCAGCACGTGAAAGCCGCCCACCTCGC
GATAGCCGTCCGCCACGTCGTGACGGGGACCGTCGTCCACCGCGCTCGACAAATTAA

Upstream 100 bases:

>100_bases
CAACGTCCTAGTCCGTTTCTTCTTTTTTAAGGAAAATGATTAGACATTCGTTGGAATGAGTTACATAATAAACTAGATAA
GGTTTGAGAGGAGAATGTAC

Downstream 100 bases:

>100_bases
TTATTCTGGCCATCGTCTTGGTGCGATGGCCTTTTTCATAGGAGTGAGAATATGGAATGGATGACATTAGAGAACGGTGT
CCGAATCATCATCGAACCAA

Product: polyribonucleotide nucleotidyltransferase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase

Number of amino acids: Translated: 738; Mature: 738

Protein sequence:

>738_residues
MLGTKQVFSTEWGGRPLSVEVGQLAKQANGSALVRYGDTVVLATVTASKAPKDLDFFPLTVNYEEKLYSVGKIPGGFLRR
EGRPGENAILTSRLIDRPIRPLFPDGFRHDIQVMIYVMSNDPDCSAEMAGMLGTSIALSISDVPFDGPIAGVTVGRVDGE
FVINPTTAQLEKTDLELQVAGTSTAINMVEAGANEVPEDVMLESILFGHEEIKRLIAFQQEIVEAVGKPKFEYTVSKYDE
ALTAELEAARPEIVEAVQVEEKHARDEAINEVIAKYVAMYEARLADEPSKLGEVSGILNKFVKDEVRRLITVEKVRPDGR
RPDVIRPLASEVGLLPRAHGVGLFTRGQTQVMSVATLGVIGDAQIIDGIGLEENKRFMHHYNFPPFSVGEARPVRAPGRR
EIGHGALGERALLPIIPKEADFPYTIRLVSEVLESNGSSSQASICGSTLALMDAGVPIKAPVAGIAMGLIMEGEHYTVLT
DIQGLEDHLGDMDFKVAGTKDGITALQMDIKIAGITREILEEALEQARVGRLHILDHMLETLETPRTQLSKYAPKIVTMT
INPDKIRDVIGPGGKMINSIIDQTGVKIDIEQDGTVFIASTDQEGIDLAMSMIGDIVREVVVGEVYDATVRRIEKFGAFV
ELFKGKDALVHVSEFSLERVANVEDVVKLGDSIQVKVTEIDDKGRVNASRKALILEGLSPEEREAYEAKRKAARESRPPR
DSRPPRRDGDRRPPRSTN

Sequences:

>Translated_738_residues
MLGTKQVFSTEWGGRPLSVEVGQLAKQANGSALVRYGDTVVLATVTASKAPKDLDFFPLTVNYEEKLYSVGKIPGGFLRR
EGRPGENAILTSRLIDRPIRPLFPDGFRHDIQVMIYVMSNDPDCSAEMAGMLGTSIALSISDVPFDGPIAGVTVGRVDGE
FVINPTTAQLEKTDLELQVAGTSTAINMVEAGANEVPEDVMLESILFGHEEIKRLIAFQQEIVEAVGKPKFEYTVSKYDE
ALTAELEAARPEIVEAVQVEEKHARDEAINEVIAKYVAMYEARLADEPSKLGEVSGILNKFVKDEVRRLITVEKVRPDGR
RPDVIRPLASEVGLLPRAHGVGLFTRGQTQVMSVATLGVIGDAQIIDGIGLEENKRFMHHYNFPPFSVGEARPVRAPGRR
EIGHGALGERALLPIIPKEADFPYTIRLVSEVLESNGSSSQASICGSTLALMDAGVPIKAPVAGIAMGLIMEGEHYTVLT
DIQGLEDHLGDMDFKVAGTKDGITALQMDIKIAGITREILEEALEQARVGRLHILDHMLETLETPRTQLSKYAPKIVTMT
INPDKIRDVIGPGGKMINSIIDQTGVKIDIEQDGTVFIASTDQEGIDLAMSMIGDIVREVVVGEVYDATVRRIEKFGAFV
ELFKGKDALVHVSEFSLERVANVEDVVKLGDSIQVKVTEIDDKGRVNASRKALILEGLSPEEREAYEAKRKAARESRPPR
DSRPPRRDGDRRPPRSTN
>Mature_738_residues
MLGTKQVFSTEWGGRPLSVEVGQLAKQANGSALVRYGDTVVLATVTASKAPKDLDFFPLTVNYEEKLYSVGKIPGGFLRR
EGRPGENAILTSRLIDRPIRPLFPDGFRHDIQVMIYVMSNDPDCSAEMAGMLGTSIALSISDVPFDGPIAGVTVGRVDGE
FVINPTTAQLEKTDLELQVAGTSTAINMVEAGANEVPEDVMLESILFGHEEIKRLIAFQQEIVEAVGKPKFEYTVSKYDE
ALTAELEAARPEIVEAVQVEEKHARDEAINEVIAKYVAMYEARLADEPSKLGEVSGILNKFVKDEVRRLITVEKVRPDGR
RPDVIRPLASEVGLLPRAHGVGLFTRGQTQVMSVATLGVIGDAQIIDGIGLEENKRFMHHYNFPPFSVGEARPVRAPGRR
EIGHGALGERALLPIIPKEADFPYTIRLVSEVLESNGSSSQASICGSTLALMDAGVPIKAPVAGIAMGLIMEGEHYTVLT
DIQGLEDHLGDMDFKVAGTKDGITALQMDIKIAGITREILEEALEQARVGRLHILDHMLETLETPRTQLSKYAPKIVTMT
INPDKIRDVIGPGGKMINSIIDQTGVKIDIEQDGTVFIASTDQEGIDLAMSMIGDIVREVVVGEVYDATVRRIEKFGAFV
ELFKGKDALVHVSEFSLERVANVEDVVKLGDSIQVKVTEIDDKGRVNASRKALILEGLSPEEREAYEAKRKAARESRPPR
DSRPPRRDGDRRPPRSTN

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain

Homologues:

Organism=Homo sapiens, GI188528628, Length=717, Percent_Identity=37.5174337517434, Blast_Score=428, Evalue=1e-120,
Organism=Escherichia coli, GI145693187, Length=683, Percent_Identity=48.3162518301611, Blast_Score=644, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115534063, Length=715, Percent_Identity=34.8251748251748, Blast_Score=371, Evalue=1e-103,
Organism=Drosophila melanogaster, GI281362905, Length=709, Percent_Identity=37.37658674189, Blast_Score=420, Evalue=1e-117,
Organism=Drosophila melanogaster, GI24651641, Length=709, Percent_Identity=37.37658674189, Blast_Score=420, Evalue=1e-117,
Organism=Drosophila melanogaster, GI24651643, Length=709, Percent_Identity=37.37658674189, Blast_Score=420, Evalue=1e-117,
Organism=Drosophila melanogaster, GI161079377, Length=660, Percent_Identity=37.1212121212121, Blast_Score=386, Evalue=1e-107,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): PNP_EXISA (C4L6J6)

Other databases:

- EMBL:   CP001615
- RefSeq:   YP_002887320.1
- ProteinModelPortal:   C4L6J6
- GeneID:   7871104
- GenomeReviews:   CP001615_GR
- KEGG:   eat:EAT1b_2961
- OMA:   YGETVVL
- ProtClustDB:   CLSK2489465
- GO:   GO:0005739
- HAMAP:   MF_01595
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:1.10.10.400
- PANTHER:   PTHR11252
- PIRSF:   PIRSF005499
- SMART:   SM00322
- SMART:   SM00316
- TIGRFAMs:   TIGR03591

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1; SSF46915 3_ExoRNase; SSF55666 3_ExoRNase; SSF50249 Nucleic_acid_OB; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.8

Molecular weight: Translated: 80710; Mature: 80710

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS50084 KH_TYPE_1; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLGTKQVFSTEWGGRPLSVEVGQLAKQANGSALVRYGDTVVLATVTASKAPKDLDFFPLT
CCCCCCHHCCCCCCCCEEEHHHHHHHHCCCCEEEEECCEEEEEEEECCCCCCCCCEEEEE
VNYEEKLYSVGKIPGGFLRREGRPGENAILTSRLIDRPIRPLFPDGFRHDIQVMIYVMSN
ECHHHHHHHHCCCCCHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEECC
DPDCSAEMAGMLGTSIALSISDVPFDGPIAGVTVGRVDGEFVINPTTAQLEKTDLELQVA
CCCCHHHHHHHHCCEEEEEEECCCCCCCCCCEEEEECCCEEEECCCHHHEECCCEEEEEE
GTSTAINMVEAGANEVPEDVMLESILFGHEEIKRLIAFQQEIVEAVGKPKFEYTVSKYDE
CCHHHHHHHHCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHH
ALTAELEAARPEIVEAVQVEEKHARDEAINEVIAKYVAMYEARLADEPSKLGEVSGILNK
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
FVKDEVRRLITVEKVRPDGRRPDVIRPLASEVGLLPRAHGVGLFTRGQTQVMSVATLGVI
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCEEEECCCHHHHHHHHHCCC
GDAQIIDGIGLEENKRFMHHYNFPPFSVGEARPVRAPGRREIGHGALGERALLPIIPKEA
CCHHHHCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
DFPYTIRLVSEVLESNGSSSQASICGSTLALMDAGVPIKAPVAGIAMGLIMEGEHYTVLT
CCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHEECCCCEEEEE
DIQGLEDHLGDMDFKVAGTKDGITALQMDIKIAGITREILEEALEQARVGRLHILDHMLE
CCCCHHHHHCCCCEEEECCCCCCEEEEEEEEEEHHHHHHHHHHHHHHHCCHHHHHHHHHH
TLETPRTQLSKYAPKIVTMTINPDKIRDVIGPGGKMINSIIDQTGVKIDIEQDGTVFIAS
HHCCCHHHHHHCCCEEEEEEECHHHHHHHHCCCHHHHHHHHHHCCCEEEEECCCEEEEEE
TDQEGIDLAMSMIGDIVREVVVGEVYDATVRRIEKFGAFVELFKGKDALVHVSEFSLERV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHH
ANVEDVVKLGDSIQVKVTEIDDKGRVNASRKALILEGLSPEEREAYEAKRKAARESRPPR
CCHHHHHHCCCCEEEEEEEECCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCC
DSRPPRRDGDRRPPRSTN
CCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MLGTKQVFSTEWGGRPLSVEVGQLAKQANGSALVRYGDTVVLATVTASKAPKDLDFFPLT
CCCCCCHHCCCCCCCCEEEHHHHHHHHCCCCEEEEECCEEEEEEEECCCCCCCCCEEEEE
VNYEEKLYSVGKIPGGFLRREGRPGENAILTSRLIDRPIRPLFPDGFRHDIQVMIYVMSN
ECHHHHHHHHCCCCCHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEECC
DPDCSAEMAGMLGTSIALSISDVPFDGPIAGVTVGRVDGEFVINPTTAQLEKTDLELQVA
CCCCHHHHHHHHCCEEEEEEECCCCCCCCCCEEEEECCCEEEECCCHHHEECCCEEEEEE
GTSTAINMVEAGANEVPEDVMLESILFGHEEIKRLIAFQQEIVEAVGKPKFEYTVSKYDE
CCHHHHHHHHCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHH
ALTAELEAARPEIVEAVQVEEKHARDEAINEVIAKYVAMYEARLADEPSKLGEVSGILNK
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
FVKDEVRRLITVEKVRPDGRRPDVIRPLASEVGLLPRAHGVGLFTRGQTQVMSVATLGVI
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCEEEECCCHHHHHHHHHCCC
GDAQIIDGIGLEENKRFMHHYNFPPFSVGEARPVRAPGRREIGHGALGERALLPIIPKEA
CCHHHHCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
DFPYTIRLVSEVLESNGSSSQASICGSTLALMDAGVPIKAPVAGIAMGLIMEGEHYTVLT
CCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHEECCCCEEEEE
DIQGLEDHLGDMDFKVAGTKDGITALQMDIKIAGITREILEEALEQARVGRLHILDHMLE
CCCCHHHHHCCCCEEEECCCCCCEEEEEEEEEEHHHHHHHHHHHHHHHCCHHHHHHHHHH
TLETPRTQLSKYAPKIVTMTINPDKIRDVIGPGGKMINSIIDQTGVKIDIEQDGTVFIAS
HHCCCHHHHHHCCCEEEEEEECHHHHHHHHCCCHHHHHHHHHHCCCEEEEECCCEEEEEE
TDQEGIDLAMSMIGDIVREVVVGEVYDATVRRIEKFGAFVELFKGKDALVHVSEFSLERV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHH
ANVEDVVKLGDSIQVKVTEIDDKGRVNASRKALILEGLSPEEREAYEAKRKAARESRPPR
CCHHHHHHCCCCEEEEEEEECCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCC
DSRPPRRDGDRRPPRSTN
CCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA