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The map label for this gene is truB [H]

Identifier: 229918671

GI number: 229918671

Start: 2918469

End: 2919377

Strand: Direct

Name: truB [H]

Synonym: EAT1b_2958

Alternate gene names: 229918671

Gene position: 2918469-2919377 (Clockwise)

Preceding gene: 229918670

Following gene: 229918672

Centisome position: 97.29

GC content: 50.94

Gene sequence:

>909_bases
TTGGAACCGAACGGAGTGCTCGTACTCGATAAAGATGCTGGCATGACGAGTCATGACTGCGTATTTAAATTACGAAAATT
GTTCCAGACGAAAAAGGTCGGACACACAGGTACACTTGACCCGGAAGTGACAGGCGTCCTACCCATCTGTTTAGGGCGTG
CCACGAAGCTGTCGCGTTTTATCACGGATGAAGGGAAACGTTACGTGGCCGAGGTGACGATTGGTACCGCCACGATGACC
GAGGATGCGCATGGCGAGGTCGTCGCACAGAAATCAGTCACACCGAACGATTTGGACGAATCGCAGATTGACGAGGCGAT
GCGTCAATTGACGGGGATGATTGAACAAATCCCGCCGTACTACTCAGCGGTCAAAGTAAACGGGAAGAAGTTGTACGAGT
ATGCACGGAAAGGACAGACCGTCGAACGGCCTCGACGCACGGTCGAAATTCATCGTCTGGAGCGGACGAGTGAATTGACA
TTTGAAGCGGATGTCTGTCGCTTCCGAATGGAAATCGACTGTGGGAAAGGGACGTATATTCGAACGCTCGCCGTTCAGAT
CGGGGAGAAGCTCGGTTACCCTGCCCATATGAGCGAACTCAGACGGACGAGCTCAGGAACGTTTGAGGAAGAGAACGCGG
TATCGCTCGAAACGTTACAGTCTCTTGAAACGGTCGAAGAACGATTGGCGCATGTGATCCCACTTGAGGATGTCATCAAA
CGCTGGCCGGCAATCACGATTCCAAAGAATCGGGAGCATTATATTCGGAATGGCGGAAAGCTGAACGGCGTTTCGCTCGA
ATTTGAGTTATTTACTGTCTATAATGAAGAAGGAATCCCTCTTGCTTTATATCGAAGACAAGAAGGAACGACAGACGCTA
CAGTCGAGGTCATGCTGGCCATCGACTAA

Upstream 100 bases:

>100_bases
TATGGTAGCCATATCGATTCGTTGATTCGTGATTTGAACAAAGATCAATAATTTGAAAGGGGAGACGTCGTTCTCCCTTT
TTTTATATGGAGGGATGAAA

Downstream 100 bases:

>100_bases
GGGGGAATTTGACAGTATGGAAACAAGACATCTCACCTATCCAGAAACACCGGACGTGTATCCGTCGGTCATGGTACTCG
GTTTCTTCGACGGTGTTCAT

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase [H]

Number of amino acids: Translated: 302; Mature: 302

Protein sequence:

>302_residues
MEPNGVLVLDKDAGMTSHDCVFKLRKLFQTKKVGHTGTLDPEVTGVLPICLGRATKLSRFITDEGKRYVAEVTIGTATMT
EDAHGEVVAQKSVTPNDLDESQIDEAMRQLTGMIEQIPPYYSAVKVNGKKLYEYARKGQTVERPRRTVEIHRLERTSELT
FEADVCRFRMEIDCGKGTYIRTLAVQIGEKLGYPAHMSELRRTSSGTFEEENAVSLETLQSLETVEERLAHVIPLEDVIK
RWPAITIPKNREHYIRNGGKLNGVSLEFELFTVYNEEGIPLALYRRQEGTTDATVEVMLAID

Sequences:

>Translated_302_residues
MEPNGVLVLDKDAGMTSHDCVFKLRKLFQTKKVGHTGTLDPEVTGVLPICLGRATKLSRFITDEGKRYVAEVTIGTATMT
EDAHGEVVAQKSVTPNDLDESQIDEAMRQLTGMIEQIPPYYSAVKVNGKKLYEYARKGQTVERPRRTVEIHRLERTSELT
FEADVCRFRMEIDCGKGTYIRTLAVQIGEKLGYPAHMSELRRTSSGTFEEENAVSLETLQSLETVEERLAHVIPLEDVIK
RWPAITIPKNREHYIRNGGKLNGVSLEFELFTVYNEEGIPLALYRRQEGTTDATVEVMLAID
>Mature_302_residues
MEPNGVLVLDKDAGMTSHDCVFKLRKLFQTKKVGHTGTLDPEVTGVLPICLGRATKLSRFITDEGKRYVAEVTIGTATMT
EDAHGEVVAQKSVTPNDLDESQIDEAMRQLTGMIEQIPPYYSAVKVNGKKLYEYARKGQTVERPRRTVEIHRLERTSELT
FEADVCRFRMEIDCGKGTYIRTLAVQIGEKLGYPAHMSELRRTSSGTFEEENAVSLETLQSLETVEERLAHVIPLEDVIK
RWPAITIPKNREHYIRNGGKLNGVSLEFELFTVYNEEGIPLALYRRQEGTTDATVEVMLAID

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs [H]

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI21040257, Length=259, Percent_Identity=32.4324324324324, Blast_Score=115, Evalue=3e-26,
Organism=Homo sapiens, GI4503337, Length=209, Percent_Identity=30.1435406698565, Blast_Score=90, Evalue=3e-18,
Organism=Homo sapiens, GI215599015, Length=209, Percent_Identity=30.1435406698565, Blast_Score=90, Evalue=3e-18,
Organism=Escherichia coli, GI2367200, Length=275, Percent_Identity=38.9090909090909, Blast_Score=189, Evalue=2e-49,
Organism=Caenorhabditis elegans, GI17553978, Length=266, Percent_Identity=27.0676691729323, Blast_Score=97, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6323204, Length=266, Percent_Identity=29.6992481203008, Blast_Score=107, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6324037, Length=140, Percent_Identity=39.2857142857143, Blast_Score=89, Evalue=8e-19,
Organism=Drosophila melanogaster, GI281364189, Length=210, Percent_Identity=30.4761904761905, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI281364187, Length=210, Percent_Identity=30.4761904761905, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI281364185, Length=210, Percent_Identity=30.4761904761905, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI281364183, Length=210, Percent_Identity=30.4761904761905, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI62471759, Length=210, Percent_Identity=30.4761904761905, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI17975520, Length=210, Percent_Identity=30.4761904761905, Blast_Score=94, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR014780 [H]

Pfam domain/function: PF01509 TruB_N [H]

EC number: 4.2.1.70

Molecular weight: Translated: 34022; Mature: 34022

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEPNGVLVLDKDAGMTSHDCVFKLRKLFQTKKVGHTGTLDPEVTGVLPICLGRATKLSRF
CCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCHHHHHHH
ITDEGKRYVAEVTIGTATMTEDAHGEVVAQKSVTPNDLDESQIDEAMRQLTGMIEQIPPY
HHCCCCEEEEEEEECCEEECCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
YSAVKVNGKKLYEYARKGQTVERPRRTVEIHRLERTSELTFEADVCRFRMEIDCGKGTYI
HHEEEECHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEEEECCCCCHH
RTLAVQIGEKLGYPAHMSELRRTSSGTFEEENAVSLETLQSLETVEERLAHVIPLEDVIK
HHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHH
RWPAITIPKNREHYIRNGGKLNGVSLEFELFTVYNEEGIPLALYRRQEGTTDATVEVMLA
HCCEEECCCCCHHHHHCCCEECCEEEEEEEEEEECCCCCEEEEEECCCCCCCCEEEEEEE
ID
EC
>Mature Secondary Structure
MEPNGVLVLDKDAGMTSHDCVFKLRKLFQTKKVGHTGTLDPEVTGVLPICLGRATKLSRF
CCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCHHHHHHH
ITDEGKRYVAEVTIGTATMTEDAHGEVVAQKSVTPNDLDESQIDEAMRQLTGMIEQIPPY
HHCCCCEEEEEEEECCEEECCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
YSAVKVNGKKLYEYARKGQTVERPRRTVEIHRLERTSELTFEADVCRFRMEIDCGKGTYI
HHEEEECHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEEEECCCCCHH
RTLAVQIGEKLGYPAHMSELRRTSSGTFEEENAVSLETLQSLETVEERLAHVIPLEDVIK
HHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHH
RWPAITIPKNREHYIRNGGKLNGVSLEFELFTVYNEEGIPLALYRRQEGTTDATVEVMLA
HCCEEECCCCCHHHHHCCCEECCEEEEEEEEEEECCCCCEEEEEECCCCCCCCEEEEEEE
ID
EC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA