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The map label for this gene is nusA [H]

Identifier: 229918666

GI number: 229918666

Start: 2914096

End: 2915259

Strand: Direct

Name: nusA [H]

Synonym: EAT1b_2953

Alternate gene names: 229918666

Gene position: 2914096-2915259 (Clockwise)

Preceding gene: 229918665

Following gene: 229918667

Centisome position: 97.14

GC content: 48.37

Gene sequence:

>1164_bases
ATGGGTCCACAATTACTCACTACAATCGATCAGATTGCCAAAGAAAAAGGAATCGACAAAAATATCATTATCGATGCACT
CGAACAAGCGCTTATTTCTGCGTATCGTCGCAACGTGGCGAACCCGAACGAGCACGTGAAAGTCGAGTTTGATCAGGTGA
CGGGGGATATCCGTGTCTATGCACTTTTAGAAGTCGTAGAGCGCCTCACGCAACCGGACCAACAGCTCTCACTCGAAGAA
GCACATGAAATCGATCCGAACTATGAGCTCGGTGATTTCCATAAAGAAGAAGTCACTCCGAGTGATTTCGGACGCGTCGC
GGCGATGACAGCTAAACAAGTCGTCACACAGAAGATGCGTGAAGCGGAGCGCGAGCGCATCTACAACCACTTCGCTGACA
AAGAAGACGAAATCATGACAGGAATCGTGGAGCGTTTCGACCCACGCAACCTGTATATCGGTCTCGAGAACAACATCGAA
GCCGTATTGACACCGAATGAACAGATGCCGAACGAGTCATACCAAATCCATGACCGCATCAAAGTATACGTGACAAAAGT
AGATTCAACGACAAAAGGTTCAGGTGCTGCGATTCAAGTTTCGCGTACGCATCCGGGACTTTTGCGTCGTTTGTTCGAAC
TCGAAGTTCCTGAGATTTCAAACGGGACAGTCGACGTGATGTCAGTATCGCGTGAAGCGGGTGACCGTTCGAAAATCGCT
GTACACTCGGATATCGTCGACCCGGTCGGCGCATGTGTCGGACCGAAAGGACAACGTGTTCAAACAATCGTCGACGAATT
GAACGGCGAGAAAATTGATATTGTCCGCTGGTCTGAAGACCCGAAAGAGTTCGTTCGTAACGCACTCAGCCCGGCTCAGG
TCGTTGCGGTATATGTCAACGAGCCAGCAAAATCGACGACAGTCGTCGTACCGGACTATCAATTGTCACTCGCAATCGGG
AAACGTGGTCAAAACGCCCGTCTCGCTGCGAAATTGACAGGATGGAAAATCGACATCAAGAGTGAAACGGATGCCGAAGC
ACTTGATTTGTTCGATACGTTCGCTGAAAAAGTTGAATCAGAACCAAAAATTGTTCAAAATGAAGTAGAACAAGACTTAT
CAGATGCTACAGTTGAAACGACTGCGGTGAAAGAAGAGGAATGA

Upstream 100 bases:

>100_bases
AGACACGCATCAAGACAAGAAAAAAAGCGGTATCGCTACCGGTAGACAAAATTGCACAAGCCCGCTTAGCGGTCACGTTT
AGTTAAGGAGAGGGAAGAAG

Downstream 100 bases:

>100_bases
CCATGAAGCAAAAGAAACTTCCTTTGCGTAAGTGTGTGGTCACACAGGAAATGCTCCCGAAAAAAGAATTGATTCGTGTC
GTTCGGACGCCTGAAGGTGA

Product: NusA antitermination factor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 387; Mature: 386

Protein sequence:

>387_residues
MGPQLLTTIDQIAKEKGIDKNIIIDALEQALISAYRRNVANPNEHVKVEFDQVTGDIRVYALLEVVERLTQPDQQLSLEE
AHEIDPNYELGDFHKEEVTPSDFGRVAAMTAKQVVTQKMREAERERIYNHFADKEDEIMTGIVERFDPRNLYIGLENNIE
AVLTPNEQMPNESYQIHDRIKVYVTKVDSTTKGSGAAIQVSRTHPGLLRRLFELEVPEISNGTVDVMSVSREAGDRSKIA
VHSDIVDPVGACVGPKGQRVQTIVDELNGEKIDIVRWSEDPKEFVRNALSPAQVVAVYVNEPAKSTTVVVPDYQLSLAIG
KRGQNARLAAKLTGWKIDIKSETDAEALDLFDTFAEKVESEPKIVQNEVEQDLSDATVETTAVKEEE

Sequences:

>Translated_387_residues
MGPQLLTTIDQIAKEKGIDKNIIIDALEQALISAYRRNVANPNEHVKVEFDQVTGDIRVYALLEVVERLTQPDQQLSLEE
AHEIDPNYELGDFHKEEVTPSDFGRVAAMTAKQVVTQKMREAERERIYNHFADKEDEIMTGIVERFDPRNLYIGLENNIE
AVLTPNEQMPNESYQIHDRIKVYVTKVDSTTKGSGAAIQVSRTHPGLLRRLFELEVPEISNGTVDVMSVSREAGDRSKIA
VHSDIVDPVGACVGPKGQRVQTIVDELNGEKIDIVRWSEDPKEFVRNALSPAQVVAVYVNEPAKSTTVVVPDYQLSLAIG
KRGQNARLAAKLTGWKIDIKSETDAEALDLFDTFAEKVESEPKIVQNEVEQDLSDATVETTAVKEEE
>Mature_386_residues
GPQLLTTIDQIAKEKGIDKNIIIDALEQALISAYRRNVANPNEHVKVEFDQVTGDIRVYALLEVVERLTQPDQQLSLEEA
HEIDPNYELGDFHKEEVTPSDFGRVAAMTAKQVVTQKMREAERERIYNHFADKEDEIMTGIVERFDPRNLYIGLENNIEA
VLTPNEQMPNESYQIHDRIKVYVTKVDSTTKGSGAAIQVSRTHPGLLRRLFELEVPEISNGTVDVMSVSREAGDRSKIAV
HSDIVDPVGACVGPKGQRVQTIVDELNGEKIDIVRWSEDPKEFVRNALSPAQVVAVYVNEPAKSTTVVVPDYQLSLAIGK
RGQNARLAAKLTGWKIDIKSETDAEALDLFDTFAEKVESEPKIVQNEVEQDLSDATVETTAVKEEE

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=382, Percent_Identity=35.6020942408377, Blast_Score=252, Evalue=2e-68,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 43262; Mature: 43131

Theoretical pI: Translated: 4.46; Mature: 4.46

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGPQLLTTIDQIAKEKGIDKNIIIDALEQALISAYRRNVANPNEHVKVEFDQVTGDIRVY
CCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHH
ALLEVVERLTQPDQQLSLEEAHEIDPNYELGDFHKEEVTPSDFGRVAAMTAKQVVTQKMR
HHHHHHHHHCCCCHHCCHHHHHCCCCCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHH
EAERERIYNHFADKEDEIMTGIVERFDPRNLYIGLENNIEAVLTPNEQMPNESYQIHDRI
HHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEEECCCEEEEECCCCCCCCCCEEEECEE
KVYVTKVDSTTKGSGAAIQVSRTHPGLLRRLFELEVPEISNGTVDVMSVSREAGDRSKIA
EEEEEEECCCCCCCCCEEEEECCCHHHHHHHHHCCCCCCCCCEEEEEEECHHCCCCCEEE
VHSDIVDPVGACVGPKGQRVQTIVDELNGEKIDIVRWSEDPKEFVRNALSPAQVVAVYVN
EEHHHHHHHHHHCCCCCCHHHHHHHHCCCCEEEEEECCCCHHHHHHHCCCHHHEEEEEEC
EPAKSTTVVVPDYQLSLAIGKRGQNARLAAKLTGWKIDIKSETDAEALDLFDTFAEKVES
CCCCCCEEEECCCEEEEEECCCCCCCEEEEEEECEEEEECCCCCHHHHHHHHHHHHHHCC
EPKIVQNEVEQDLSDATVETTAVKEEE
CCHHHHHHHHHHHHHCEEEEEECCCCC
>Mature Secondary Structure 
GPQLLTTIDQIAKEKGIDKNIIIDALEQALISAYRRNVANPNEHVKVEFDQVTGDIRVY
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHH
ALLEVVERLTQPDQQLSLEEAHEIDPNYELGDFHKEEVTPSDFGRVAAMTAKQVVTQKMR
HHHHHHHHHCCCCHHCCHHHHHCCCCCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHH
EAERERIYNHFADKEDEIMTGIVERFDPRNLYIGLENNIEAVLTPNEQMPNESYQIHDRI
HHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEEECCCEEEEECCCCCCCCCCEEEECEE
KVYVTKVDSTTKGSGAAIQVSRTHPGLLRRLFELEVPEISNGTVDVMSVSREAGDRSKIA
EEEEEEECCCCCCCCCEEEEECCCHHHHHHHHHCCCCCCCCCEEEEEEECHHCCCCCEEE
VHSDIVDPVGACVGPKGQRVQTIVDELNGEKIDIVRWSEDPKEFVRNALSPAQVVAVYVN
EEHHHHHHHHHHCCCCCCHHHHHHHHCCCCEEEEEECCCCHHHHHHHCCCHHHEEEEEEC
EPAKSTTVVVPDYQLSLAIGKRGQNARLAAKLTGWKIDIKSETDAEALDLFDTFAEKVES
CCCCCCEEEECCCEEEEEECCCCCCCEEEEEEECEEEEECCCCCHHHHHHHHHHHHHHCC
EPKIVQNEVEQDLSDATVETTAVKEEE
CCHHHHHHHHHHHHHCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]