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The map label for this gene is priA [H]

Identifier: 229918579

GI number: 229918579

Start: 2830553

End: 2832877

Strand: Direct

Name: priA [H]

Synonym: EAT1b_2866

Alternate gene names: 229918579

Gene position: 2830553-2832877 (Clockwise)

Preceding gene: 229918578

Following gene: 229918580

Centisome position: 94.36

GC content: 51.44

Gene sequence:

>2325_bases
ATGATTGCACATGTACATGTCGATGCACCGGTGATCACGATTGATCGTCCGTTTGATTACGAAGTACCTGAACAATTTGA
GGCTTTGATTGAACCAGGCATGCGCGTCTCTGTCCCATTCGGGTCCCGTCGTCTGCTCGGGATCGTCGTCGGTACGTCAG
ATGGGACGAGAGATGGATTAAAACCGTTAGAAGCGCTGTTAGATGAGGAATCTTCACTCACCGATGAACTGCTCGCTTTA
TCCATTCATGTGAAAGAGACGACACTTTGCTTTCGTTCATCCGCCTTGCTCGCGATGCTTCCGGCCGCGCTCAAGGTGAG
TTATGACAAAGAAATCAAAGAAGGTCATGTGCCTGATGGGGTCCGCCTCGGAACGCATTTATCGGAGTATCCAAAAGAAG
TCCAATCGGTCATTCTTGGACTCGCAAAAAAAGGGGAGCTGATCTTAGCGCCCGTCTTAAAAGAAAAGAAGACTGTAAAG
ACCGACGTCGTCGTCGAGCTCCTCGATGCAACGGTCGTGCCAAAACGTGCGAAAAAGCAACTCGAAGCGGTCGACCTACT
AAAGGAGACCCCACGCATGTATTGGTCGGCACTACGTCAAATTGGTCTGACTCGTCCACAACTGCAAAAACTTGCCGACC
TCGGAGCGGTTCGATTGACCGAGGTCGAGTTGAACCGTGACCCGTACGCGACAATCGAGCAAGTCGAGGAATCGGTTCAA
TTGAATGACAGTCAGGCAAGTGCCGTCTTGGCCATACAGGAGGCTGAAGCGGGTGAGACGTTACTCGTCCACGGTGTGAC
GGGGAGCGGGAAGACCGAAGTCTATTTAGAATCAATCGGACAAGTCATCGATGAAGGCAAGCAGGCCATCTTACTCGTTC
CTGAAATCAGTTTGACCCCGATGATGGTCAAACGCTTCAAGCGTCGTTTCGGCGATCGGGTCGCCGTGTTGCACAGTGCT
CTGTCTAAAGGGGAGAAGTACGACGAGTGGCGTAAGATTAAACGAAATGAAGTCGACGTCGTCGTCGGTGCCCGTTCTGC
CATCTTTGCCCCGCTCGACCGACTCGGTCTGATCATCTTAGATGAAGAGCACGAGACGACGTACAAGCAAGAAGAAAATC
CCCGTTATCATGCGCGCGACGTCGCCATCTGGCGGGCTCGTTATCACGGTTGTCCTGTCGTGTTAGGCAGTGCGACCCCG
TCACTTGAATCGTATGCCCGGGCACAAAAAGGCGTCTATCGCTATTTGCATTTGAAAGAACGGTATGGCGGTGAGATGCC
ACCGGTCCATATCATTGACATGCGCCGGGAGTTGGCGAAGGGCAATACGACGATGTTCTCGGAAGAATTGTTCCAAGGCA
TTCTCGACCGGATCGAAAAAAAAGAGCAGTGCGTCATTTTATTGAACCGTCGTGGGTTCACGACGTTCGTCATGTGCCGG
GACTGTGGTGAAGGCATGACATGTCCCCATTGTGCCGTCAATTTGACATATCATCAGTATGGAGACCGATTGAAGTGTCA
CTATTGTGGTTACGAGGTCGGCATGCCGTCGACATGTCCATCCTGCAACAGTAAGAAAATCAAACAGTTCGGGTCGGGCA
CGCAAAAAATCGAAACGGAACTGTTGAACCGGATTCCAGAAGCGCGCATCATCCGAATGGACCAGGATACGACGTCTCGA
AAAGGATCACATGAGCAGTTGTTGCGTCGTTTCGAAGAAGGGGAGGCCGACATCTTACTTGGCACGCAGATGATTGCCAA
GGGGCTCGACTTCCCAAACGTCACCCTCGTCGGGGTACTCGCGGCAGATGCGACACTCGGGATGCCTGACTTTCGGGCGA
CCGAACGCACCTTCCAACTTGTCACGCAAGTGGCAGGAAGAGCCGGGCGCGGAAAACTTCCGGGTGAGGCGTTCGTCCAA
ACGTATAACCCGGACCATTATGTGATCGAGACTGCGAGTGAACATGATTATGAGACATTTTTCGCCAAAGAGATGGGACT
CAGACAAGTCGGGAACCATCCGCCTTACTGGTTTTTAACGCTCGTCACGTTTTCGGCGAAAAATCCTCTTGTCGCCAAGG
CAGAAGCAGAATTGTTCGCATCAGACTTTTTACAAGCGCACGTGGAGAACAGCCGTCTGAATGGACCGATGCCCGCGCCG
TTATCGAAGTTGAAGAACGCCTATCGTTATCAAGTATTCATTAAAACGAAACAACCCGATACACTTTACGCCGAGCTGGC
ACGATTACAGCAAGCGAGAGCCAAAGCAATCAGCAAGAAAGAATATCAAATGAGTATCGATGTGAACCCATACGTATTCA
TGTGA

Upstream 100 bases:

>100_bases
TCGTCTTGTTCCATGCAGATGGGACGACACATCGACTTCCGAAACAGTCGAAGAAACAGTTAGCGTTCGAGTTGCTCACA
CACTTTAAGGAAGCGGTCCG

Downstream 100 bases:

>100_bases
GGTGGAGAGACATGTATAAAGTAGTAGAAGTACCAAATGACATTTTGCGCGTAACGTGCGAACCGGTGACGAAGTTTGAT
AAAAAATTGCGTCAGACTGT

Product: primosomal protein N'

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 774; Mature: 774

Protein sequence:

>774_residues
MIAHVHVDAPVITIDRPFDYEVPEQFEALIEPGMRVSVPFGSRRLLGIVVGTSDGTRDGLKPLEALLDEESSLTDELLAL
SIHVKETTLCFRSSALLAMLPAALKVSYDKEIKEGHVPDGVRLGTHLSEYPKEVQSVILGLAKKGELILAPVLKEKKTVK
TDVVVELLDATVVPKRAKKQLEAVDLLKETPRMYWSALRQIGLTRPQLQKLADLGAVRLTEVELNRDPYATIEQVEESVQ
LNDSQASAVLAIQEAEAGETLLVHGVTGSGKTEVYLESIGQVIDEGKQAILLVPEISLTPMMVKRFKRRFGDRVAVLHSA
LSKGEKYDEWRKIKRNEVDVVVGARSAIFAPLDRLGLIILDEEHETTYKQEENPRYHARDVAIWRARYHGCPVVLGSATP
SLESYARAQKGVYRYLHLKERYGGEMPPVHIIDMRRELAKGNTTMFSEELFQGILDRIEKKEQCVILLNRRGFTTFVMCR
DCGEGMTCPHCAVNLTYHQYGDRLKCHYCGYEVGMPSTCPSCNSKKIKQFGSGTQKIETELLNRIPEARIIRMDQDTTSR
KGSHEQLLRRFEEGEADILLGTQMIAKGLDFPNVTLVGVLAADATLGMPDFRATERTFQLVTQVAGRAGRGKLPGEAFVQ
TYNPDHYVIETASEHDYETFFAKEMGLRQVGNHPPYWFLTLVTFSAKNPLVAKAEAELFASDFLQAHVENSRLNGPMPAP
LSKLKNAYRYQVFIKTKQPDTLYAELARLQQARAKAISKKEYQMSIDVNPYVFM

Sequences:

>Translated_774_residues
MIAHVHVDAPVITIDRPFDYEVPEQFEALIEPGMRVSVPFGSRRLLGIVVGTSDGTRDGLKPLEALLDEESSLTDELLAL
SIHVKETTLCFRSSALLAMLPAALKVSYDKEIKEGHVPDGVRLGTHLSEYPKEVQSVILGLAKKGELILAPVLKEKKTVK
TDVVVELLDATVVPKRAKKQLEAVDLLKETPRMYWSALRQIGLTRPQLQKLADLGAVRLTEVELNRDPYATIEQVEESVQ
LNDSQASAVLAIQEAEAGETLLVHGVTGSGKTEVYLESIGQVIDEGKQAILLVPEISLTPMMVKRFKRRFGDRVAVLHSA
LSKGEKYDEWRKIKRNEVDVVVGARSAIFAPLDRLGLIILDEEHETTYKQEENPRYHARDVAIWRARYHGCPVVLGSATP
SLESYARAQKGVYRYLHLKERYGGEMPPVHIIDMRRELAKGNTTMFSEELFQGILDRIEKKEQCVILLNRRGFTTFVMCR
DCGEGMTCPHCAVNLTYHQYGDRLKCHYCGYEVGMPSTCPSCNSKKIKQFGSGTQKIETELLNRIPEARIIRMDQDTTSR
KGSHEQLLRRFEEGEADILLGTQMIAKGLDFPNVTLVGVLAADATLGMPDFRATERTFQLVTQVAGRAGRGKLPGEAFVQ
TYNPDHYVIETASEHDYETFFAKEMGLRQVGNHPPYWFLTLVTFSAKNPLVAKAEAELFASDFLQAHVENSRLNGPMPAP
LSKLKNAYRYQVFIKTKQPDTLYAELARLQQARAKAISKKEYQMSIDVNPYVFM
>Mature_774_residues
MIAHVHVDAPVITIDRPFDYEVPEQFEALIEPGMRVSVPFGSRRLLGIVVGTSDGTRDGLKPLEALLDEESSLTDELLAL
SIHVKETTLCFRSSALLAMLPAALKVSYDKEIKEGHVPDGVRLGTHLSEYPKEVQSVILGLAKKGELILAPVLKEKKTVK
TDVVVELLDATVVPKRAKKQLEAVDLLKETPRMYWSALRQIGLTRPQLQKLADLGAVRLTEVELNRDPYATIEQVEESVQ
LNDSQASAVLAIQEAEAGETLLVHGVTGSGKTEVYLESIGQVIDEGKQAILLVPEISLTPMMVKRFKRRFGDRVAVLHSA
LSKGEKYDEWRKIKRNEVDVVVGARSAIFAPLDRLGLIILDEEHETTYKQEENPRYHARDVAIWRARYHGCPVVLGSATP
SLESYARAQKGVYRYLHLKERYGGEMPPVHIIDMRRELAKGNTTMFSEELFQGILDRIEKKEQCVILLNRRGFTTFVMCR
DCGEGMTCPHCAVNLTYHQYGDRLKCHYCGYEVGMPSTCPSCNSKKIKQFGSGTQKIETELLNRIPEARIIRMDQDTTSR
KGSHEQLLRRFEEGEADILLGTQMIAKGLDFPNVTLVGVLAADATLGMPDFRATERTFQLVTQVAGRAGRGKLPGEAFVQ
TYNPDHYVIETASEHDYETFFAKEMGLRQVGNHPPYWFLTLVTFSAKNPLVAKAEAELFASDFLQAHVENSRLNGPMPAP
LSKLKNAYRYQVFIKTKQPDTLYAELARLQQARAKAISKKEYQMSIDVNPYVFM

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=754, Percent_Identity=35.4111405835544, Blast_Score=405, Evalue=1e-114,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 86833; Mature: 86833

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAHVHVDAPVITIDRPFDYEVPEQFEALIEPGMRVSVPFGSRRLLGIVVGTSDGTRDGL
CEEEEEECCEEEEECCCCCCCCCHHHHHHHCCCCEEECCCCCCEEEEEEEECCCCCCCHH
KPLEALLDEESSLTDELLALSIHVKETTLCFRSSALLAMLPAALKVSYDKEIKEGHVPDG
HHHHHHHCCCCCHHHHHEEEEEEEEHHHHHHHCCHHHHHHHHHHHCCCCHHHHCCCCCCC
VRLGTHLSEYPKEVQSVILGLAKKGELILAPVLKEKKTVKTDVVVELLDATVVPKRAKKQ
CCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEHHHCCHHHHHHHHHHHHHHHCCCHHHHHH
LEAVDLLKETPRMYWSALRQIGLTRPQLQKLADLGAVRLTEVELNRDPYATIEQVEESVQ
HHHHHHHHHCHHHHHHHHHHHCCCCHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHH
LNDSQASAVLAIQEAEAGETLLVHGVTGSGKTEVYLESIGQVIDEGKQAILLVPEISLTP
CCCCCCEEEEEEEECCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCH
MMVKRFKRRFGDRVAVLHSALSKGEKYDEWRKIKRNEVDVVVGARSAIFAPLDRLGLIIL
HHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHCCCCEEEEEECHHHHHHCHHHCCEEEE
DEEHETTYKQEENPRYHARDVAIWRARYHGCPVVLGSATPSLESYARAQKGVYRYLHLKE
ECCCCCCCCCCCCCCEEHHHHHEEHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHH
RYGGEMPPVHIIDMRRELAKGNTTMFSEELFQGILDRIEKKEQCVILLNRRGFTTFVMCR
HCCCCCCCEEHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCEEEEEEE
DCGEGMTCPHCAVNLTYHQYGDRLKCHYCGYEVGMPSTCPSCNSKKIKQFGSGTQKIETE
CCCCCCCCCCCEEEEEHHHCCCCEEEEECCEECCCCCCCCCCCCHHHHHHCCCHHHHHHH
LLNRIPEARIIRMDQDTTSRKGSHEQLLRRFEEGEADILLGTQMIAKGLDFPNVTLVGVL
HHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHCCCCEEEECHHHHHCCCCCCCEEEEEEE
AADATLGMPDFRATERTFQLVTQVAGRAGRGKLPGEAFVQTYNPDHYVIETASEHDYETF
ECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHEEECCCCEEEEECCCCCCHHHH
FAKEMGLRQVGNHPPYWFLTLVTFSAKNPLVAKAEAELFASDFLQAHVENSRLNGPMPAP
HHHHHHHHHHCCCCCEEEEEEEEECCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCH
LSKLKNAYRYQVFIKTKQPDTLYAELARLQQARAKAISKKEYQMSIDVNPYVFM
HHHHCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEC
>Mature Secondary Structure
MIAHVHVDAPVITIDRPFDYEVPEQFEALIEPGMRVSVPFGSRRLLGIVVGTSDGTRDGL
CEEEEEECCEEEEECCCCCCCCCHHHHHHHCCCCEEECCCCCCEEEEEEEECCCCCCCHH
KPLEALLDEESSLTDELLALSIHVKETTLCFRSSALLAMLPAALKVSYDKEIKEGHVPDG
HHHHHHHCCCCCHHHHHEEEEEEEEHHHHHHHCCHHHHHHHHHHHCCCCHHHHCCCCCCC
VRLGTHLSEYPKEVQSVILGLAKKGELILAPVLKEKKTVKTDVVVELLDATVVPKRAKKQ
CCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEHHHCCHHHHHHHHHHHHHHHCCCHHHHHH
LEAVDLLKETPRMYWSALRQIGLTRPQLQKLADLGAVRLTEVELNRDPYATIEQVEESVQ
HHHHHHHHHCHHHHHHHHHHHCCCCHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHH
LNDSQASAVLAIQEAEAGETLLVHGVTGSGKTEVYLESIGQVIDEGKQAILLVPEISLTP
CCCCCCEEEEEEEECCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCH
MMVKRFKRRFGDRVAVLHSALSKGEKYDEWRKIKRNEVDVVVGARSAIFAPLDRLGLIIL
HHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHCCCCEEEEEECHHHHHHCHHHCCEEEE
DEEHETTYKQEENPRYHARDVAIWRARYHGCPVVLGSATPSLESYARAQKGVYRYLHLKE
ECCCCCCCCCCCCCCEEHHHHHEEHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHH
RYGGEMPPVHIIDMRRELAKGNTTMFSEELFQGILDRIEKKEQCVILLNRRGFTTFVMCR
HCCCCCCCEEHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCEEEEEEE
DCGEGMTCPHCAVNLTYHQYGDRLKCHYCGYEVGMPSTCPSCNSKKIKQFGSGTQKIETE
CCCCCCCCCCCEEEEEHHHCCCCEEEEECCEECCCCCCCCCCCCHHHHHHCCCHHHHHHH
LLNRIPEARIIRMDQDTTSRKGSHEQLLRRFEEGEADILLGTQMIAKGLDFPNVTLVGVL
HHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHCCCCEEEECHHHHHCCCCCCCEEEEEEE
AADATLGMPDFRATERTFQLVTQVAGRAGRGKLPGEAFVQTYNPDHYVIETASEHDYETF
ECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHEEECCCCEEEEECCCCCCHHHH
FAKEMGLRQVGNHPPYWFLTLVTFSAKNPLVAKAEAELFASDFLQAHVENSRLNGPMPAP
HHHHHHHHHHCCCCCEEEEEEEEECCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCH
LSKLKNAYRYQVFIKTKQPDTLYAELARLQQARAKAISKKEYQMSIDVNPYVFM
HHHHCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9534248; 9384377; 9086272 [H]