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The map label for this gene is pyrE [H]

Identifier: 229918572

GI number: 229918572

Start: 2824098

End: 2824724

Strand: Direct

Name: pyrE [H]

Synonym: EAT1b_2859

Alternate gene names: 229918572

Gene position: 2824098-2824724 (Clockwise)

Preceding gene: 229918571

Following gene: 229918573

Centisome position: 94.14

GC content: 51.99

Gene sequence:

>627_bases
ATGAAACAAATCGCTGAGGCACTTTTACAGATTGAAGCCGTCACACTATCACCGACTGAGCCGTATACATGGTCGAGCGG
ATTGAAGAGTCCAATCTACTGCGACAATCGACTTACACTCAGTTTTCCGAATGTGCGTCAAGTCATTGTCGATGGATTGG
TGAAGTCAGTGAATCCGACTGAAGTCGATGTGATTGCAGGGACGGCCACGGCAGGGATCCCTCACGGGGCATTGCTCGCT
GACCGACTCGGTCTCCCGTTCATTTATGTCCGGTCGAGTGCAAAAGGGCATGGGAAAGGTAATCAAATTGAAGGGAAACT
CGAGGCAGGCGCTCGAGTGCTCGTCATCGAGGATTTATTGTCGACAGGGATGTCGAGTATCGATGCTGCCCAAGCGATAC
GTGAGGCGGGCGGTGAAGTCACTCGAATTCAAGCCATCTTCTCGTACGGATTACCAGTTCTCGAGAAGAATCTAGCCGAT
GCAAATCTGTCGGCGACGTCGTTGACGACGTTCTCAGAATTAATTGAAGTGGCCGAAAATACGGGCGTCCTCAACCGTTC
GGAGCTCGATTCGCTTCGTGACTGGCAAGAAGACCCTCGTGCCTGGAGCGACCTCGTCACCTCTTAA

Upstream 100 bases:

>100_bases
CTGGCGGGTGTTCACTCAATCGTCGTCGGTCGACCGATTACACGTGCGACTGATCCGATGGCCATGTACGACCAATTTAT
GAAGGAATGGGGACAAATGA

Downstream 100 bases:

>100_bases
GAATCCACCTTCGGGCGGATTCTTTTTCGATTCGGCATATGTCATACTTCATGGTAAAATAACGGAAGTGATTCGAAAAG
GAGAGGCATATGGCGTATTC

Product: orotate phosphoribosyltransferase

Products: NA

Alternate protein names: OPRT; OPRTase [H]

Number of amino acids: Translated: 208; Mature: 208

Protein sequence:

>208_residues
MKQIAEALLQIEAVTLSPTEPYTWSSGLKSPIYCDNRLTLSFPNVRQVIVDGLVKSVNPTEVDVIAGTATAGIPHGALLA
DRLGLPFIYVRSSAKGHGKGNQIEGKLEAGARVLVIEDLLSTGMSSIDAAQAIREAGGEVTRIQAIFSYGLPVLEKNLAD
ANLSATSLTTFSELIEVAENTGVLNRSELDSLRDWQEDPRAWSDLVTS

Sequences:

>Translated_208_residues
MKQIAEALLQIEAVTLSPTEPYTWSSGLKSPIYCDNRLTLSFPNVRQVIVDGLVKSVNPTEVDVIAGTATAGIPHGALLA
DRLGLPFIYVRSSAKGHGKGNQIEGKLEAGARVLVIEDLLSTGMSSIDAAQAIREAGGEVTRIQAIFSYGLPVLEKNLAD
ANLSATSLTTFSELIEVAENTGVLNRSELDSLRDWQEDPRAWSDLVTS
>Mature_208_residues
MKQIAEALLQIEAVTLSPTEPYTWSSGLKSPIYCDNRLTLSFPNVRQVIVDGLVKSVNPTEVDVIAGTATAGIPHGALLA
DRLGLPFIYVRSSAKGHGKGNQIEGKLEAGARVLVIEDLLSTGMSSIDAAQAIREAGGEVTRIQAIFSYGLPVLEKNLAD
ANLSATSLTTFSELIEVAENTGVLNRSELDSLRDWQEDPRAWSDLVTS

Specific function: Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP) [H]

COG id: COG0461

COG function: function code F; Orotate phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily [H]

Homologues:

Organism=Caenorhabditis elegans, GI17508631, Length=199, Percent_Identity=27.6381909547739, Blast_Score=76, Evalue=1e-14,
Organism=Drosophila melanogaster, GI17933654, Length=201, Percent_Identity=24.8756218905473, Blast_Score=71, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004467
- InterPro:   IPR023031
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00156 Pribosyltran [H]

EC number: =2.4.2.10 [H]

Molecular weight: Translated: 22278; Mature: 22278

Theoretical pI: Translated: 4.46; Mature: 4.46

Prosite motif: PS00012 PHOSPHOPANTETHEINE ; PS00626 RCC1_2 ; PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQIAEALLQIEAVTLSPTEPYTWSSGLKSPIYCDNRLTLSFPNVRQVIVDGLVKSVNPT
CHHHHHHHHHHHHEEECCCCCCCCCCCCCCCEEECCEEEEECCHHHHHHHHHHHHCCCCC
EVDVIAGTATAGIPHGALLADRLGLPFIYVRSSAKGHGKGNQIEGKLEAGARVLVIEDLL
EEEEEECCCCCCCCCHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCEEEEHHHHH
STGMSSIDAAQAIREAGGEVTRIQAIFSYGLPVLEKNLADANLSATSLTTFSELIEVAEN
HCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
TGVLNRSELDSLRDWQEDPRAWSDLVTS
CCCCCHHHHHHHHHHHHCHHHHHHHHCC
>Mature Secondary Structure
MKQIAEALLQIEAVTLSPTEPYTWSSGLKSPIYCDNRLTLSFPNVRQVIVDGLVKSVNPT
CHHHHHHHHHHHHEEECCCCCCCCCCCCCCCEEECCEEEEECCHHHHHHHHHHHHCCCCC
EVDVIAGTATAGIPHGALLADRLGLPFIYVRSSAKGHGKGNQIEGKLEAGARVLVIEDLL
EEEEEECCCCCCCCCHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCEEEEHHHHH
STGMSSIDAAQAIREAGGEVTRIQAIFSYGLPVLEKNLADANLSATSLTTFSELIEVAEN
HCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
TGVLNRSELDSLRDWQEDPRAWSDLVTS
CCCCCHHHHHHHHHHHHCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]