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The map label for this gene is pyrF [H]

Identifier: 229918571

GI number: 229918571

Start: 2823410

End: 2824114

Strand: Direct

Name: pyrF [H]

Synonym: EAT1b_2858

Alternate gene names: 229918571

Gene position: 2823410-2824114 (Clockwise)

Preceding gene: 229918570

Following gene: 229918572

Centisome position: 94.12

GC content: 53.33

Gene sequence:

>705_bases
ATGCGCAACTTGTATCTCGCCCTTGACGTGGAAACGCGGGACGAAGCGTTTCATTTGTTGAAACGATTTTCAAACCGACC
GGCTGTTAAAGTCGGGATGGAACTCTTTTATCGGGAAGGCGCACGCTTTGTCGAAGAATTGGTCGACGGGGGGTATCCGG
TCTTTCTCGATGTGAAAGTACACGATATCCCGGAGACGGCACGCCGTACGATGAGACAAATCGGAAAACTCGGGGTGGCT
GTGACGAATGTCCACACATTAGGTGGCGAAAAGATGATGCGCTATGCGTTAGACGGGTTACGCCAAGAGAATGATACGAC
TCGATTGATTGGGGTCACACAGTTGACGTCGACAGATGAGCGCATGATGCAGATAGAGCTCGGGATTGCCGGACGACTCG
ACCAAGCCGTTCTCAGACAAGCGACACTTGCGAAGCAGGCGGGACTTGACGGAGTGGTCGCATCCGTTCAGGAAGCGAAA
GCGATTCATCAGAAACTCGGGGACACGTTCATGACGGTCACGCCGGGCATCCGACTCGGTGGAACCGAGGATGATCAAGT
CCGTATCGCGACGCCGCATGAAGCGAGACTGGCGGGTGTTCACTCAATCGTCGTCGGTCGACCGATTACACGTGCGACTG
ATCCGATGGCCATGTACGACCAATTTATGAAGGAATGGGGACAAATGAATGAAACAAATCGCTGA

Upstream 100 bases:

>100_bases
TTTGAAAATCCATACGTCTGCCAGGAAATCATCGAAGCGTTACCGGCACGACTCGATTTACTCGGAATCGAACATATTTT
GGATGTGAGGGGGAAGGCCT

Downstream 100 bases:

>100_bases
GGCACTTTTACAGATTGAAGCCGTCACACTATCACCGACTGAGCCGTATACATGGTCGAGCGGATTGAAGAGTCCAATCT
ACTGCGACAATCGACTTACA

Product: orotidine 5'-phosphate decarboxylase

Products: NA

Alternate protein names: OMP decarboxylase; OMPDCase; OMPdecase [H]

Number of amino acids: Translated: 234; Mature: 234

Protein sequence:

>234_residues
MRNLYLALDVETRDEAFHLLKRFSNRPAVKVGMELFYREGARFVEELVDGGYPVFLDVKVHDIPETARRTMRQIGKLGVA
VTNVHTLGGEKMMRYALDGLRQENDTTRLIGVTQLTSTDERMMQIELGIAGRLDQAVLRQATLAKQAGLDGVVASVQEAK
AIHQKLGDTFMTVTPGIRLGGTEDDQVRIATPHEARLAGVHSIVVGRPITRATDPMAMYDQFMKEWGQMNETNR

Sequences:

>Translated_234_residues
MRNLYLALDVETRDEAFHLLKRFSNRPAVKVGMELFYREGARFVEELVDGGYPVFLDVKVHDIPETARRTMRQIGKLGVA
VTNVHTLGGEKMMRYALDGLRQENDTTRLIGVTQLTSTDERMMQIELGIAGRLDQAVLRQATLAKQAGLDGVVASVQEAK
AIHQKLGDTFMTVTPGIRLGGTEDDQVRIATPHEARLAGVHSIVVGRPITRATDPMAMYDQFMKEWGQMNETNR
>Mature_234_residues
MRNLYLALDVETRDEAFHLLKRFSNRPAVKVGMELFYREGARFVEELVDGGYPVFLDVKVHDIPETARRTMRQIGKLGVA
VTNVHTLGGEKMMRYALDGLRQENDTTRLIGVTQLTSTDERMMQIELGIAGRLDQAVLRQATLAKQAGLDGVVASVQEAK
AIHQKLGDTFMTVTPGIRLGGTEDDQVRIATPHEARLAGVHSIVVGRPITRATDPMAMYDQFMKEWGQMNETNR

Specific function: Catalyzes the decarboxylation of orotidine 5'- monophosphate (OMP) to uridine 5'-monophosphate (UMP) [H]

COG id: COG0284

COG function: function code F; Orotidine-5'-phosphate decarboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the OMP decarboxylase family. Type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787537, Length=215, Percent_Identity=42.3255813953488, Blast_Score=144, Evalue=4e-36,

Paralogues:

None

Copy number: 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR014732
- InterPro:   IPR018089
- InterPro:   IPR001754
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00215 OMPdecase [H]

EC number: =4.1.1.23 [H]

Molecular weight: Translated: 26168; Mature: 26168

Theoretical pI: Translated: 7.19; Mature: 7.19

Prosite motif: PS00156 OMPDECASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNLYLALDVETRDEAFHLLKRFSNRPAVKVGMELFYREGARFVEELVDGGYPVFLDVKV
CCCEEEEEEECCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEE
HDIPETARRTMRQIGKLGVAVTNVHTLGGEKMMRYALDGLRQENDTTRLIGVTQLTSTDE
CCCCHHHHHHHHHHHHHCHHHEEEECCCHHHHHHHHHHHHCCCCCCEEEEEEEECCCCHH
RMMQIELGIAGRLDQAVLRQATLAKQAGLDGVVASVQEAKAIHQKLGDTFMTVTPGIRLG
HHEEEECCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCEEEEECCCEEEC
GTEDDQVRIATPHEARLAGVHSIVVGRPITRATDPMAMYDQFMKEWGQMNETNR
CCCCCCEEEECCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MRNLYLALDVETRDEAFHLLKRFSNRPAVKVGMELFYREGARFVEELVDGGYPVFLDVKV
CCCEEEEEEECCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEE
HDIPETARRTMRQIGKLGVAVTNVHTLGGEKMMRYALDGLRQENDTTRLIGVTQLTSTDE
CCCCHHHHHHHHHHHHHCHHHEEEECCCHHHHHHHHHHHHCCCCCCEEEEEEEECCCCHH
RMMQIELGIAGRLDQAVLRQATLAKQAGLDGVVASVQEAKAIHQKLGDTFMTVTPGIRLG
HHEEEECCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCEEEEECCCEEEC
GTEDDQVRIATPHEARLAGVHSIVVGRPITRATDPMAMYDQFMKEWGQMNETNR
CCCCCCEEEECCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]