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The map label for this gene is murF [H]

Identifier: 229918547

GI number: 229918547

Start: 2796910

End: 2798214

Strand: Direct

Name: murF [H]

Synonym: EAT1b_2834

Alternate gene names: 229918547

Gene position: 2796910-2798214 (Clockwise)

Preceding gene: 229918546

Following gene: 229918548

Centisome position: 93.23

GC content: 52.11

Gene sequence:

>1305_bases
ATGGAACAACTGACAGTTCAAAAATTAGCATCATGGTTTGGGATGGATCTACATGACGAGCGTCTCGTCGAACGGGTCGC
CACAGATTCGAGAGATGCAAACGAGGCGGGCCTCTTCGTCCCAATCGTCGGGGCGCGAGTCGACGGCCATGACTACATCG
AGGCGGCCGTTGAAAAAGGTGCGATTGTCACGTTCTGGGAAGAGGGTCGCCCTCAACCAGACAATCTGGTTGTCATCCCT
GTGAAATCAACGGTCGAGGCATTGCAAACAGCAGCGGCCCGATATTTAGAAGAGATCAATCCGCTCGTCGTCGCCATTAC
GGGCTCGAATGGGAAGACGTCGACAAAAGACATGGTCGCTGTCGTGCTCGAGGAGAAGTTCAAGACGCATAAGACAGCCG
GAAATTTCAACTCGGACGTCGGCTTACCACTTACCGTTCTCAGAATGCCGCGGGATACGGAAGTAGCCGTCCTCGAGATG
GGAATGAACGGATTCGGTCAAATCGAGACGCTGTCAAAACTTGCTAAGCCGGATGTGGCGTTTGTGACGAATATCGGGGA
GTCTCACGGTGAACAAGTCGGCGGGCGGACAGGTATCGCCAAGGCGAAACTTGAGATCAAGGCCGGTCTCAAAGCGGACG
GTCGCTTGATCGTCGATGCGGATGAGCCACTTCTTTCTGGTCAAGGCTATCGCGTCGGATACGATGAGACGGCGGATGGG
AAAATTGAAGTGATCGAAACGACGTTTGACGCATCGATCTTTAAATATAACGGTGACTTGTATGAACTGAAGGTGCTCGG
TGCCCATCAGATTCGAAACGCAGCCTATGCGATTGCGTGTGGACTTGCGTTCGGTCTCGATCATGCGATGATTCAACGTG
GCTTAGACCGAATCAAGTTGACGTCGATGCGAATGGAAAAACAGATGTTCGGGGAAGCACACCTCATTAACGATGCCTAC
AACGCGAGTCCGACGTCGATGATTGCAGCGATTGAGACGATCAAGTCGCTTGAAGGGTTCTCGAAGCGTGTGCTCGTCCT
TGGAGATATGTACGAGTTAGGTGCCGACGAACAAATGCAACACGAACAAGTCGGTGGCGCCGTGACGTCTCCCGTGACCG
ATTGTATTCTCGTCGGCGACAAAGCAAAATGGATTGCCGATGGAATTCAAGATTCACGAGTGAACGTTAAAATGGTCCCG
ACGGTTGAGGAAGCGAAGGCGCTCCTTCATCATTATCAACGACCGGATACTGTGGTATTGTTAAAGGCATCTCGAGGACT
GGCGCTTGAGCGTCTTGTTCAATAA

Upstream 100 bases:

>100_bases
AGGTGCCTCCTTGAAAGTGAAGTTGGAGACACCGTCGAATTAACGCACGAGATGAAGAGGGCTGCGGCTCTCTTTTCCTA
TGTAAGAGGAGGAAGTTTGT

Downstream 100 bases:

>100_bases
ATAGAGTAGTGAGGTAGCAACATGTTAATTAGTTTAATCGTATTAATCAGTTCATTATTAAGTGTCTTGATCGTGATGCC
GCGAGCCATCCCGGCATTGC

Product: UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diaminopimelate/D-alanyl-D-alanyl ligase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 434; Mature: 434

Protein sequence:

>434_residues
MEQLTVQKLASWFGMDLHDERLVERVATDSRDANEAGLFVPIVGARVDGHDYIEAAVEKGAIVTFWEEGRPQPDNLVVIP
VKSTVEALQTAAARYLEEINPLVVAITGSNGKTSTKDMVAVVLEEKFKTHKTAGNFNSDVGLPLTVLRMPRDTEVAVLEM
GMNGFGQIETLSKLAKPDVAFVTNIGESHGEQVGGRTGIAKAKLEIKAGLKADGRLIVDADEPLLSGQGYRVGYDETADG
KIEVIETTFDASIFKYNGDLYELKVLGAHQIRNAAYAIACGLAFGLDHAMIQRGLDRIKLTSMRMEKQMFGEAHLINDAY
NASPTSMIAAIETIKSLEGFSKRVLVLGDMYELGADEQMQHEQVGGAVTSPVTDCILVGDKAKWIADGIQDSRVNVKMVP
TVEEAKALLHHYQRPDTVVLLKASRGLALERLVQ

Sequences:

>Translated_434_residues
MEQLTVQKLASWFGMDLHDERLVERVATDSRDANEAGLFVPIVGARVDGHDYIEAAVEKGAIVTFWEEGRPQPDNLVVIP
VKSTVEALQTAAARYLEEINPLVVAITGSNGKTSTKDMVAVVLEEKFKTHKTAGNFNSDVGLPLTVLRMPRDTEVAVLEM
GMNGFGQIETLSKLAKPDVAFVTNIGESHGEQVGGRTGIAKAKLEIKAGLKADGRLIVDADEPLLSGQGYRVGYDETADG
KIEVIETTFDASIFKYNGDLYELKVLGAHQIRNAAYAIACGLAFGLDHAMIQRGLDRIKLTSMRMEKQMFGEAHLINDAY
NASPTSMIAAIETIKSLEGFSKRVLVLGDMYELGADEQMQHEQVGGAVTSPVTDCILVGDKAKWIADGIQDSRVNVKMVP
TVEEAKALLHHYQRPDTVVLLKASRGLALERLVQ
>Mature_434_residues
MEQLTVQKLASWFGMDLHDERLVERVATDSRDANEAGLFVPIVGARVDGHDYIEAAVEKGAIVTFWEEGRPQPDNLVVIP
VKSTVEALQTAAARYLEEINPLVVAITGSNGKTSTKDMVAVVLEEKFKTHKTAGNFNSDVGLPLTVLRMPRDTEVAVLEM
GMNGFGQIETLSKLAKPDVAFVTNIGESHGEQVGGRTGIAKAKLEIKAGLKADGRLIVDADEPLLSGQGYRVGYDETADG
KIEVIETTFDASIFKYNGDLYELKVLGAHQIRNAAYAIACGLAFGLDHAMIQRGLDRIKLTSMRMEKQMFGEAHLINDAY
NASPTSMIAAIETIKSLEGFSKRVLVLGDMYELGADEQMQHEQVGGAVTSPVTDCILVGDKAKWIADGIQDSRVNVKMVP
TVEEAKALLHHYQRPDTVVLLKASRGLALERLVQ

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=429, Percent_Identity=33.3333333333333, Blast_Score=164, Evalue=9e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 47249; Mature: 47249

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQLTVQKLASWFGMDLHDERLVERVATDSRDANEAGLFVPIVGARVDGHDYIEAAVEKG
CCCHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCEEEEEEEEEECCHHHHHHHHHCC
AIVTFWEEGRPQPDNLVVIPVKSTVEALQTAAARYLEEINPLVVAITGSNGKTSTKDMVA
CEEEEECCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHH
VVLEEKFKTHKTAGNFNSDVGLPLTVLRMPRDTEVAVLEMGMNGFGQIETLSKLAKPDVA
HHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCEEEEEECCCCCCHHHHHHHHCCCCEE
FVTNIGESHGEQVGGRTGIAKAKLEIKAGLKADGRLIVDADEPLLSGQGYRVGYDETADG
EEECCCHHHHHHCCCCCCCEEEEEEEEECCCCCCEEEEECCCCEECCCCEEECCCCCCCC
KIEVIETTFDASIFKYNGDLYELKVLGAHQIRNAAYAIACGLAFGLDHAMIQRGLDRIKL
EEEEEEEEECCEEEEECCCEEEEEEECHHHHHHHHHHHHHHHHHCCCHHHHHCCHHHHHH
TSMRMEKQMFGEAHLINDAYNASPTSMIAAIETIKSLEGFSKRVLVLGDMYELGADEQMQ
HHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECHHHCCCCHHHH
HEQVGGAVTSPVTDCILVGDKAKWIADGIQDSRVNVKMVPTVEEAKALLHHYQRPDTVVL
HHHHCCCHHCCCCCEEEECCCHHHHHCCCCCCCEEEEECCCHHHHHHHHHHCCCCCEEEE
LKASRGLALERLVQ
EECCCCCHHHHHCC
>Mature Secondary Structure
MEQLTVQKLASWFGMDLHDERLVERVATDSRDANEAGLFVPIVGARVDGHDYIEAAVEKG
CCCHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCEEEEEEEEEECCHHHHHHHHHCC
AIVTFWEEGRPQPDNLVVIPVKSTVEALQTAAARYLEEINPLVVAITGSNGKTSTKDMVA
CEEEEECCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHH
VVLEEKFKTHKTAGNFNSDVGLPLTVLRMPRDTEVAVLEMGMNGFGQIETLSKLAKPDVA
HHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCEEEEEECCCCCCHHHHHHHHCCCCEE
FVTNIGESHGEQVGGRTGIAKAKLEIKAGLKADGRLIVDADEPLLSGQGYRVGYDETADG
EEECCCHHHHHHCCCCCCCEEEEEEEEECCCCCCEEEEECCCCEECCCCEEECCCCCCCC
KIEVIETTFDASIFKYNGDLYELKVLGAHQIRNAAYAIACGLAFGLDHAMIQRGLDRIKL
EEEEEEEEECCEEEEECCCEEEEEEECHHHHHHHHHHHHHHHHHCCCHHHHHCCHHHHHH
TSMRMEKQMFGEAHLINDAYNASPTSMIAAIETIKSLEGFSKRVLVLGDMYELGADEQMQ
HHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECHHHCCCCHHHH
HEQVGGAVTSPVTDCILVGDKAKWIADGIQDSRVNVKMVPTVEEAKALLHHYQRPDTVVL
HHHHCCCHHCCCCCEEEECCCHHHHHCCCCCCCEEEEECCCHHHHHHHHHHCCCCCEEEE
LKASRGLALERLVQ
EECCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]